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Citations for Takara Bio :
451 - 500 of 773 citations for Vertical Electrophoresis system since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: Yeast two-hybrid assays were performed according to the manual of Matchmaker Gold Yeast two-hybrid system (Clontech). The relevant combinations of constructs were co-transformed into yeast strain AH109 using the polyethylene glycol/lithium acetate method as described in the manual ...
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bioRxiv - Plant Biology 2020Quote: ... was prepared from rice roots under drought stress using Make Your Own “Mate and Plate” Library System (Clontech). The constructs were co-transformed into Y2H Gold yeast strain and screened on DDO (SD/-Leu/-Trp ...
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bioRxiv - Neuroscience 2020Quote: The screen of a rat brain cDNA library was carried out with the GAL4-based MATCHMAKER system (Clontech) and all procedures followed the system protocols ...
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bioRxiv - Biochemistry 2021Quote: The R3C ligase construct10 was cloned into the pRZ plasmid71 using the InFusion cloning system (Takara Bio, Japan). To ensure correct length of the transcript ...
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bioRxiv - Biochemistry 2021Quote: ... All yeast two-hybrid screens and assays were performed using the BD Matchmaker system (BD Biosciences, Clontech Laboratories) utilizing the Saccharomyces cerevisiae strain AH109 essentially as described earlier (Stephan et al. ...
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bioRxiv - Developmental Biology 2020Quote: ... musculus Dyrk2 cDNA was cloned into pcDNA4/TO-C-3xFLAG using the In-fusion HD cloning system (Clontech). Dyrk2 cDNA clone #6808145 was purchased (ThermoFisher Scientific) ...
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bioRxiv - Biophysics 2021Quote: ... A total of 20 μl reaction system contains 10 μl 2X Premix Ex Taq II (TaKaRa, Dalian, China), 1 μl of 3D gene specific primer (forward ...
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bioRxiv - Cell Biology 2021Quote: ... The PCR products were cloned into the HaloTag vector based on PB533 using an in-fusion system (TaKaRa). The expression vectors of H2B-Halo and Halo-PCNA were constructed by inserting their coding sequence (Kanda et al. ...
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bioRxiv - Cell Biology 2022Quote: ... inducible cell lines were generated using the Lenti-X Tet-On-Advanced Inducible Expression System (Clontech Laboratories, Inc.) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... 2016) was inserted into the NotI site of pENTR Mito using the In-Fusion HD Cloning System (Clontech).
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bioRxiv - Microbiology 2021Quote: ... Real-time reverse transcription-PCR was performed using a Thermal Cycler Dice real-time system MRQ (Takara Bio) with TB Green Premix Ex Taq II (Tli RNaseH Plus ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Yeast 2-hybrid library screening was conducted using the Matchmaker Gold Yeast Two-Hybrid System (Takara Bio USA) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: The N-terminal domain of dTBCE (amino acid 1 to 207) was cloned using the Infusion system (Takara) into pET23B (Clontech ...
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bioRxiv - Microbiology 2020Quote: ... The qPCR reaction system consisted of 12.5 μL of 2 × SYBR Premix Ex TaqTM II (Takara, Dalian, China), 0.5 μL of upstream and downstream primers (10 mM) ...
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bioRxiv - Plant Biology 2021Quote: ... URA3: GAL1UAS–Gal1TATA–LacZ MEL1) via the Yeastmaker™ Yeast Transformation System 2 kit (Clontech, Mountain View, USA). An agarose gel image of the cDNA library is shown in Fig ...
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bioRxiv - Molecular Biology 2021Quote: ... Real-time RT-qPCR was performed on the cDNA (Thermal Cycler Dice Real Time System IIMRQ, Takara Bio) using Takara SYBR Premix Ex Taq II (Takara Bio ...
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bioRxiv - Neuroscience 2022Quote: All viruses were made using the 2nd generation lentiviral packaging systems in Lenti-X HEK293 FTT cells (Takara). Lenti-X cells were passaged maximum 3 times before being used for virus production in HEK media (High glucose DMEM with 4 mM GlutaMAX ...
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bioRxiv - Microbiology 2023Quote: ... Fluorescent signals from resulting PCR products were acquired using a Thermal Cycler Dice Real Time System III (Takara). Finally ...
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bioRxiv - Microbiology 2022Quote: ... and the resultant fragments were cloned into pCM-GALK using the In-Fusion cloning system (Takara Bio, Japan). Resulting plasmid was introduced into C ...
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bioRxiv - Molecular Biology 2022Quote: ... with PowerUp™ SYBR™ using the Thermal Cycler Dice® Real Time System III (TAKARA Bio, Japan). These PCRs were run in triplicates ...
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bioRxiv - Developmental Biology 2023Quote: ... followed by insertion of a flexible linker72 at the C-terminus of EGFP using the InFusion system (Takara). α-tubulin CDS was amplified from a cDNA clone ...
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bioRxiv - Plant Biology 2023Quote: The interaction between PIF4 and MPK4 was examined using the Matchmaker gold yeast two-hybrid system (Takara Bio). PIF4 and MPK4 cDNA were cloned in the pGADT7 and pGBKT7 vectors ...
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bioRxiv - Microbiology 2023Quote: ... Fluorescent signals from resulting PCR products were acquired using a Thermal Cycler Dice Real Time System III (Takara).
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bioRxiv - Cancer Biology 2023Quote: ... TP63 cDNA constructs were cloned into a lentiviral IRES-Neo vector using the In-Fusion cloning system (Takara). A 3×FLAG tag was added to TP63 at the N- or C-terminus ...
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bioRxiv - Cell Biology 2020Quote: ... we took advantage of the In-Fusion® HD Cloning system (Takara Bio Europe, Saint-Germain-en-Laye, FR) to generate a PiggyBac™ transposon system (PB ...
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bioRxiv - Cell Biology 2020Quote: ... The RNA was reverse-transcribed to single-stranded cDNA using the AMV Reverse Transcription System (Takara, Dalian, Liaoning, China). Then ...
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bioRxiv - Developmental Biology 2021Quote: ... RT-qPCR was performed on three biological replicates using an ABI StepOne PCR detection system with SYBR Green (Takara). GmUbiquitin (SUBI-2.2 ...
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Targeted degradation of PCNA outperforms stoichiometric inhibition to result in programed cell deathbioRxiv - Cell Biology 2022Quote: Human embryonic kidney T-REx 293 cells which express the tetracycline repressor protein were purchased from Thermo Fisher Scientific and cultured in Eagle’s Minimum Essential Medium (MEM) GlutaMAX™ supplemented with 10% Tet system approved FBS (Clontech) and 5 μg/mL blasticidin ...
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bioRxiv - Genomics 2020Quote: ... using a Multi Sample Nano Dispenser (MSND, SMARTer™ ICELL8® Single-Cell System, Takara Bio USA, CA, USA). Chip wells were sealed using SmartChip Optical Imaging Film (Takara Bio USA ...
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bioRxiv - Genomics 2020Quote: RPE1 cells co-expressing 53BP1-eGFP and Lifeact-RFP were generated using two individual pIRES-Neo3 vector systems (Clontech) harboring either the coding sequence of 53BP1 (representing amino acid 1220-1711 ...
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bioRxiv - Molecular Biology 2021Quote: ... First strand cDNA was synthesized with 3.6 μg of total RNA using Make your own ‘mate & plate’ library system (Takara, Clontech) according to the manufacturer’s instruction ...
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bioRxiv - Neuroscience 2019Quote: ... The gene was cloned into pGEM-HE vector through the In-Fusion HD Cloning system (Clontech, Mountain View, CA). Amino acid sequence was identical to that in VectorBase.
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bioRxiv - Cancer Biology 2020Quote: ... phospho-specific mutant constitutively active NFATC4 17 was also cloned into the doxycycline-inducible Tet-One expression system (Clontech) to create an inducible and constitutive NFATC4 (IcNFATC4 ...
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bioRxiv - Plant Biology 2020Quote: Y2H screening was performed using Matchmaker Gold Yeast Two-Hybrid System (Takara Bio USA, Inc., Mountain View, US-CA). For bait AP_SAP11-like_PM19 or AP_SAP11-like_PM19Δ40-56 was cloned into pGBKT7 (TRP1 nutritional marker ...
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bioRxiv - Neuroscience 2020Quote: ... MEM (supplemented with non-essential amino acids) from HiMedia (India) and Tet system approved FBS was obtained from Takara Bio USA ...
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bioRxiv - Molecular Biology 2021Quote: DIE cells were cultured in growth medium consisting of IMDM basal medium with 10% Tet system-approved FBS (Clontech) and 55μM 2-mercaptoethanol ...
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bioRxiv - Plant Biology 2021Quote: ... which were inserted at the HindIII site of pMpGWB101 or pMpGWB301 using the In-fusion HD Cloning System (Clontech). To construct proMpDUO1:ST-Venus ...
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bioRxiv - Cancer Biology 2020Quote: ... or ALDH2 were cloned into the LentiV_Neo vector using the In-Fusion cloning system (Takara Bio; Cat. No. 121416). The CRISPR-resistant synonymous mutant of UBE2T and FANCL ...
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bioRxiv - Cancer Biology 2020Quote: The in vitro targeting efficiency of sgRNAs was tested using a Guide-it Complete sgRNA Screening System (Takara, 632636) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: The recombinant pGBKT7-TaPHB-1 was transformed into Y2HGold yeast strain following the Yeastmaker Yeast Transformation System 2 (TaKaRa). The transformants were screened on SD/-Trp agar plate ...
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Core Microbiota Drive the Prevalence of Extracellular Antibiotic Resistome in the Water CompartmentsbioRxiv - Microbiology 2022Quote: ... High throughput qPCR (HT-qPCR) was implemented on SmartChip PCR system including a Multisample Nanodispenser and a Cycler (Takara SmartChip™ ...
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bioRxiv - Cell Biology 2022Quote: ... The yeast was transformed with the indicated plasmids using the Matchmaker™ Yeast Transformation System 2 (Clontech, Cat#: 630439). Two plasmids containing simian virus (SV ...
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bioRxiv - Cell Biology 2022Quote: ... All lentiviral particles were produced in Lenti-X 293T cells using the Lenti-X HTS Packaging System (Takara, Japan) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... The sgRNAs were prepared following the instruction (Guide-it™ sgRNA In Vitro Transcription and Screening Systems, TaKaRa, Japan). Briefly ...
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bioRxiv - Cell Biology 2022Quote: ... The yeast two-hybrid assays were performed according to the Matchmarker Gold Yeast two-hybrid system manual from Clontech. Different variations of the constructs were co-transformed by the polyethylene glycol/ lithium acetate method into the AH109 yeast strain and selected on SD/-Leu-Trp plates ...
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bioRxiv - Plant Biology 2019Quote: ... SmCOP1 and SmHY5 respectively into the yeast strain AH109 as instructions for Matchmaker GAL4 Two-Hybrid Systems 3 (Clontech). Yeast Two-Hybrid analysis were performed following Yeast Protocols Handbook (Clontech) ...
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bioRxiv - Pathology 2019Quote: Y2H library was constructed using the Make your own “Mate & Plate” library system according to manufacturer’s manual with minor modifications (Clontech). In brief ...
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bioRxiv - Cell Biology 2019Quote: ... Using the online In-Fusion™ HD (IF) cloning system primer design website (Takara Bio USA, Mountain View, CA), we made primer sets for TMEM163 SNP ...
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bioRxiv - Developmental Biology 2020Quote: ... The insertion site of the Col2a1-tTA transgene was determined based on the Genome Walker Universal system (Takara Bio). The Col2a1-tTA transgene was inserted 16kbp downstream of Pellino2 on Chromosome 14 ...
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bioRxiv - Developmental Biology 2019Quote: ... Stable transgenic cell lines were established according to the manual of the Tet-Express inducible expression systems (Clontech, 631169). Briefly ...