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Citations for Takara Bio :
101 - 150 of 402 citations for SDS PAGE Single gel since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... PCR products were cut from agarose gels and purified using the Nucleospin gel purification kit (Takara Bio, 240609). Final plasmids were constructed using In-Fusion HD (Takara Bio ...
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bioRxiv - Immunology 2023Quote: ... linearized BF520 sequence was gel purified using NucleoSpin Gel and PCR Clean-up kit (Takara, Cat. No. 740609.5) and then purified using Ampure XP beads (Beckman Coulter ...
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bioRxiv - Microbiology 2024Quote: ... linearized GPC sequence was gel purified using NucleoSpin Gel and PCR Clean-up kit (Takara, Cat. No. 740609.5) and then purified using Ampure XP beads (Beckman Coulter ...
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bioRxiv - Immunology 2020Quote: ... and purified by gel extraction (Clontech Libraries).
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bioRxiv - Molecular Biology 2020Quote: ... The resulting band was gel-purified (Clontech) and sequenced by Sanger Sequencing ...
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bioRxiv - Developmental Biology 2021Quote: ... Single embryos were collected in 2 µl RNase free water with Recombinant RNase inhibitor (TAKARA) and ruptured with an RNase free needle ...
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bioRxiv - Microbiology 2020Quote: ... single-stranded (ss) cDNA (sscDNA) was synthesized using the SMARTer RACE 5′/3′ Kit (Takara) with a U2-complementary primer ...
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bioRxiv - Neuroscience 2020Quote: ... Single labeling involved exposure of sections to 1:25,000 or 1:100,000 anti-DSRed (Takara), 1:500 anti-rabbit IgG ...
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bioRxiv - Cancer Biology 2021Quote: ... Single nuclei candidates were selected using the ICELL8 imaging system with CellSelect Software (Takara Bio) selecting for DAPI positive nuclei and reverse transcription and sequencing library preparation was performed according to manufacturer instructions ...
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bioRxiv - Molecular Biology 2022Quote: Single-cell K562 libraries were prepared using the SMART-seq Stranded (SSS) Kit (Takara Bio), following the manufacturer instructions with modifications ...
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bioRxiv - Immunology 2023Quote: ... Full-length cDNA amplification of single cells was performed per the kit protocol (Takara Bio). The analysis of the surface markers of the positive cells was performed on FlowJo.
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bioRxiv - Microbiology 2021Quote: ... PCR products of EgGLUT1-ss gene were recovered from Agarose Gel with Agarose Gel DNA Extraction Kit (Takara, Japan), and the amplified fragments were cloned into pMD19-T vector with Mighty ta-cloning Reagent Set for Prime STAR (Takara ...
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bioRxiv - Biophysics 2019Quote: ... and the products were further purified in 3% agarose-TBE gels and subsequently reisolated using gel-purification kits (Clontech).
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bioRxiv - Cell Biology 2020Quote: ... was used to PCR amplify the PLIN2 cDNA and gel purified via NucleoSpin Gel and PCR Clean Up (Takara, Mountain View ...
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bioRxiv - Microbiology 2022Quote: Amplified spike sequence was first gel-purified using NucleoSpin Gel and PCR Clean-up kit (Takara, Cat. No. 740609.5) and then further purified using Ampure XP beads (Beckman Coulter ...
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bioRxiv - Cancer Biology 2024Quote: ... The 13 kb band was purified with gel purification kit (Takara Nucleospin® Gel and PCR Clean-up Midi). Eighty-two base pair single stranded oligos were designed according to the following structure:
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Plant pathogens convergently evolved to counteract redundant nodes of an NLR immune receptor networkbioRxiv - Plant Biology 2021Quote: ... and on a SD-Leu-Trp-Ade-His plate (ST0054, Takara Bio, USA) containing X-α-gal and supplemented with 0.2% Adenine ...
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bioRxiv - Microbiology 2020Quote: ... Diploid cells were selected with SD supplemented with -Leu/-Trp DO supplement (Clontech) (SD-Leu-Trp) ...
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bioRxiv - Systems Biology 2021Quote: ... selecting on SD -HIS agar plates (Takara Bio, 630411; Diagonal GmbH&CoKG, Y1751). The correct genomic context insertion of GFP of single colonies was confirmed by PCR using a combination of primers which also allowed the confirmation of the intron deletion strain ...
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bioRxiv - Plant Biology 2022Quote: ... Transformants were selected on Synthetic Defined (SD) media lacking Leucine and Tryptophane (Clontech). Three individual clones were grown ON in liquid cultures (lacking Leucine and Tryptophane ...
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bioRxiv - Molecular Biology 2020Quote: Primers (PAGE grade, fasmac, Appendix Table S4) were labelled with 32P-γ-ATP by T4PNK (Takara), followed by filtering through MicroSpin G-25 Columns (GE healthcare) ...
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bioRxiv - Microbiology 2020Quote: ... The 1.5 kb PCR product was isolated and purified from agarose gel using the NucleoSpin Gel and PCR Clean-up kit (Takara). The product was subjected to Sanger dideoxy sequencing at GENEWIZ (San Diego ...
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bioRxiv - Microbiology 2019Quote: ... The resulting product was purified from a 1% agarose gel using the NucleoSpin Gel and PCR Clean-up Kit (Takara) and Sanger sequenced.
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bioRxiv - Bioengineering 2020Quote: ... Ligation products with the correct size were purified by DNA agarose gel extraction using NucleoSpin Gel and PCR Clean-Up Kit (Takara, 740609.250 ...
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bioRxiv - Molecular Biology 2021Quote: ... 2 μl of each common amplicon were pooled and gel purified from a 1.5% agarose gel (Nucleospin clean-up, Takara Bio), eluting in 35 μl 10 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2019Quote: ... The specific PCR products were gel purified by using the DNA Gel Extraction Kit (Axygen, USA) and cloned to the pMD-18 vector system (Takara), and then sequenced by the Shanghai Sangon Company.
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bioRxiv - Cancer Biology 2023Quote: ... the agarose gel pieces were cut out and digested using NucleoSpin® Gel and PCR Clean-Up kit (Takara, 740609), according to the manufacturer’s protocols ...
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bioRxiv - Cell Biology 2023Quote: ... plus any amplicon smaller than the 9246 bp WT band for the large indel screen were gel purified with the NucleoSpin Gel clean up kit (Takara Bio ...
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bioRxiv - Immunology 2024Quote: ... we ran our linearized plasmid backbone and 83 bp PCR products on a 1% agarose gel and recovered each using the Gel and PCR Clean-up Kit (Takara) per the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... Digests were run on a 0.8% agarose-TAE gel and full-length plasmids were extracted with the Nucleospin PCR and Gel Cleanup Kit (Takara, 740609). A LR reaction was performed with 150 ng entry library and 150 ng pDEST_HC_Rec_Bxb_v2 ...
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bioRxiv - Molecular Biology 2020Quote: ... and Nucleospin Gel and PCR Clean-up (Takara). The sequencing library was sequenced with the Illumina Next-Seq 500 instrument (Illumina ...
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bioRxiv - Cancer Biology 2023Quote: ... followed by gel purification (Takara Bio Cat. #740609.50). Samples were then pooled and sequenced on a lane of HiSeq 4000 SE50 at the UCSF Center for Advanced Technology (CAT).
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bioRxiv - Cancer Biology 2023Quote: ... followed by gel purification (Takara Bio Cat. #740609.50). Samples were then pooled and sequenced on a lane of HiSeq 4000 SE50 at the UCSF Center for Advanced Technology (CAT).
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bioRxiv - Biochemistry 2020Quote: ... Lentivirus particles were generated using the Lenti-X Single Shot system (ClonTech, Mountain View, CA, US) in 293T-Lenti-X cells ...
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bioRxiv - Microbiology 2020Quote: ... The PCR-amplified elements were ligated in a single reaction step by In-Fusion cloning (Clontech) upstream of the HA3 tag in XmaI and AfeI sites of pLN-HA3-loxP ...
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bioRxiv - Cell Biology 2021Quote: ... Transfection was carried out using Lenti-X Packaging Single Shot (VSV-G) system (Takara Bio, 631275) according to the following protocol ...
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Werner syndrome helicase is a selective vulnerability of microsatellite instability high tumor cellsbioRxiv - Cancer Biology 2019Quote: ... Lentiviral particles were produced using the Lenti-X Single Shot system (Clontech, Mountain View, CA, US). Following lentiviral infection ...
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Satb2 acts as a gatekeeper for major developmental transitions during early vertebrate embryogenesisbioRxiv - Developmental Biology 2020Quote: Single embryos were harvested in 100ul RNA iso-plus total RNA extraction reagent (DSS Takara, India) at 80% epiboly ...
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bioRxiv - Cell Biology 2020Quote: ... The cloned plasmid was packaged into lentivirus using lenti-X-packaging-single-shots (VSVG) (Takara, #631275) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: We produced the lentivirus for expressing TIR1-9myc using Lenti-X Packaging Single Shots system (Clontech) and infected the CTCF-AID knock-in mESCs following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... Single-indexed libraries were generated using ThruPLEX® DNA-seq Kit (Takara Bio Inc., Shiga, Japan), pooled and checked again using a Bioanalyzer (Agilent High Sensitivity DNA Kit ...
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bioRxiv - Cancer Biology 2023Quote: ... and genomic DNA amplification was carried out using the PicoPLEX Single Cell WGA kit v3 (Takara) and following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... Wells containing single viable cells were automatically selected using the ICELL8 cx CellSelect v2.5 Software (Takara) with the green and not red logic ...
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bioRxiv - Plant Biology 2021Quote: ... SD growth medium w/o Ade−His−Leu−Trp− (Himedia) and supplements media (Clontech) were used for the Y2H experiment.
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bioRxiv - Plant Biology 2021Quote: ... Transformants were selected on Synthetic Defined (SD) medium lacking Leu and Trp (−2) (Clontech). Three individual transformants were grown overnight in liquid SD (−2 ...
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bioRxiv - Plant Biology 2021Quote: ... and cells with both constructs were selected on SD/-Leu/-Trp medium (Clontech, 630417). Ten colonies picked up with a tooth pick were diluted in 1ml 0.9%NaCl and spotted onto SD/-Ade/-His/-Leu/-Trp medium (Clontech ...
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bioRxiv - Molecular Biology 2020Quote: ... Amplicons were isolated from the agarose gel using the PCR cleanup and gel extraction kit (ClonTech, Takara USA, Mountain View, CA) and the nucleotide sequence of amplicons determined by Sanger direct sequencing (GENEWIZ ...
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bioRxiv - Molecular Biology 2020Quote: ... Amplicons were isolated from the agarose gel using the PCR cleanup and gel extraction kit (ClonTech, Takara USA, Mountain View, CA) and the nucleotide sequence of amplicons determined by Sanger direct sequencing (GENEWIZ ...
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bioRxiv - Microbiology 2020Quote: ... The obtained linearized plasmid was excised from the agarose gel and purified using a MiniBEST Agarose Gel DNA Extraction Kit (Takara Bio). The cohesive ends of the linearized plasmid were blunt-ended and self-ligated using a DNA Blunting Kit (Code No ...
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bioRxiv - Genomics 2023Quote: ... purified via gel extraction from a 1% agarose gel followed by cleanup using the NucleoSpin PCR clean up and gel extraction kit (Takara Bio). Linearized CROP-seq-optiI and amplified oligonucleotides were assembled using the NEBuilder HiFi DNA assembly cloning kit (NEB ...