Labshake search
Citations for Takara Bio :
1 - 50 of 402 citations for SDS PAGE Single gel since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2019Quote: ... Same volume of samples were loaded on 4-16% gradient SDS-PAGE gels and analyzed by western blot analysis using EGFP (JL-8; Clontech), penta-HIS (QIAGEN ...
-
bioRxiv - Molecular Biology 2021Quote: ... Cell lysate was mixed with SDS-PAGE loading buffer (Takara) containing 200 mM DTT ...
-
bioRxiv - Molecular Biology 2023Quote: ... SDS-PAGE and immunoblotting were as described using a GFP monoclonal antibody (Clontech).
-
bioRxiv - Cell Biology 2022Quote: Protein extracts were analyzed by SDS-PAGE and immunoblotting using α-GFP (monoclonal, Takara), α-mCherry (polyclonal ...
-
bioRxiv - Biochemistry 2019Quote: ... Protein purity and concentration were determined by SDS-PAGE and BCA protein assay kit (Takara) respectively ...
-
bioRxiv - Physiology 2022Quote: ... Cleared lysates were loaded into wells of the SDS-agarose gels (1% SeaKem Gold Agarose [Lonza, Basel, Switzerland], 30% glycerol, and 1 × Tris/glycine/SDS [TG-SDS] buffer [Takara Bio Inc.]) and then electrophoresed in a running buffer (1 × TG-SDS buffer and 10 mM 2-mercaptoethanol ...
-
bioRxiv - Cell Biology 2019Quote: ... 10 µg of the lysates were separated by SDS-PAGE and blots were probed with a 1:2,000 dilution of mouse anti-GFP antibody (Clontech), followed by 1:5000 anti-mouse Starbright Blue 700 (Bio-Rad ...
-
bioRxiv - Molecular Biology 2021Quote: ... Beads were washed twice with Pulldown Buffer and ran on SDS-PAGE followed by western blotting (anti-His Clontech 631212).
-
bioRxiv - Physiology 2022Quote: ... the beads were well-washed by column binding buffer 5 times and then incubated in 2× Protein SDS PAGE Loading (Takara) 100°C for 5 min to completely elute the proteins ...
-
bioRxiv - Cell Biology 2021Quote: ... Co-immunoprecipitation and SDS-PAGE followed by western blotting was carried out as mentioned above except TALON metal affinity resin (Takara) was used instead of α-GFP beads for 6His-Skb1 enrichment.
-
bioRxiv - Cell Biology 2022Quote: ... The samples were subjected to 4-20% SDS-PAGE and analyzed by immunoblotting with the following primary antibodies: anti-GFP mouse monoclonal antibody (JL-8, Takara), GFP rabbit polyclonal antibody (PABG1 ...
-
bioRxiv - Plant Biology 2024Quote: ... S1 and P1 fractions were loaded in denaturing buffer for SDS-PAGE separation and immunoblotted with the indicated antibodies (anti-DDB2: Molinier et al., 2008; anti-GFP: Takara-632593 ...
-
bioRxiv - Microbiology 2020Quote: ... followed by a brief centrifugation step before being resolved by SDS-PAGE and subjected to western blotting using anti-6xhis antibodies (TaKaRa: 631212), or anti-BamA POTRA antiserum (36) ...
-
bioRxiv - Microbiology 2019Quote: ... Samples were boiled for 10 min before being resolved by SDS-PAGE and subjected to western blotting using anti-6xhis antibodies (TaKaRa: 631212) as primary antibody and HRP conjugated anti-rabbit (Sigma Aldrich ...
-
bioRxiv - Biophysics 2021Quote: ... Samples were run on SDS-PAGE and analyzed by Western blot using mouse anti-mCherry primary antibody (1:2000, Takara Bio #632543) and Licor secondary antibody (Licor #926032212 ...
-
bioRxiv - Bioengineering 2019Quote: ... SD-His or SD-Trp-Ura (Clontech). Mycoplasma pneumoniae strain M129 (ATCC 29342) ...
-
bioRxiv - Synthetic Biology 2023Quote: ... SD-Trp-Ura or SD-His-Leu (Clontech Takara).
-
bioRxiv - Synthetic Biology 2023Quote: ... SD-Trp-Ura or SD-His-Leu (Clontech Takara).
-
bioRxiv - Cell Biology 2022Quote: ... were run onto 8% SDS- polyacrylamide gels that were transferred to nitrocellulose membranes which were reacted with α-His tag antibody (#631212, Clontech). Quantitation of band intensities was done with ImageLab software (BioRad) ...
-
bioRxiv - Developmental Biology 2023Quote: Single blastomeres or single blastocysts were lysed in 10X Lysis Buffer from Takara (cat.no. 635013), deposited in two Mosquito® plates ...
-
bioRxiv - Cell Biology 2020Quote: ... Minimal SD base (Clontech) and the indicated Amino Acid Dropout mix (Clontech) ...
-
bioRxiv - Plant Biology 2021Quote: ... Culture mediums SD/-Leu/-Trp and SD/-Ade/-His/-Leu/-Trp (Clontech, USA) with or without X-α-gal were used to select the positive transformants.
-
bioRxiv - Cancer Biology 2020Quote: Single-cell RNA-Sequencing (scRNA-Seq) was performed using the ICELL8® Single-Cell System (Takara Bio) using the SMARTer™ ICELL8 3’ DE Kit (Takara Bio) ...
-
A Phytochrome B-PIF4-MYC2 Module Tunes Secondary Cell Wall Thickening in Response to Shaded LightingbioRxiv - Plant Biology 2021Quote: ... and then grown on SD-Trp-Leu and SD-Trp-Leu-His plates (Clontech).
-
bioRxiv - Plant Biology 2021Quote: ... The transformants were cultivated on SD/-Leu or SD/-Leu/AbA (Aureobasidin A, Clontech, USA) medium for three days ...
-
bioRxiv - Plant Biology 2023Quote: ... The transformed yeast strains were grown on SD/-Leu and SD/-Leu/AureobasidinA media (Takara) and Y1H analysis was performed using Matchmaker® Gold Yeast One-hybrid screening system (Takara ...
-
bioRxiv - Genomics 2021Quote: ... SMART-Seq Single Cell Kit (Takara Bio) libraries were prepared following the manufacturer instructions ...
-
bioRxiv - Cancer Biology 2020Quote: Whole Genome Amplification (WGA) was performed on 300 single cells for each Time by means of SMARTer PicoPLEX Single Cell WGA kit (Takara) following manufacturer’s protocol as previously described24 ...
-
bioRxiv - Neuroscience 2022Quote: Single-cell RNA-seq libraries were constructed according to the SMART-Seq Single PLUS protocol (Takara Bio, Mountain View, CA). Frozen plates were thawed on ice ...
-
bioRxiv - Cell Biology 2023Quote: Each single-cell RNA-seq library was generated from a single FGO or a single MII oocyte using SMART-Seq HT Kit (Takara) according to the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2021Quote: ... with Lenti-X packaging single shots (Takara Bio), and concentrated using Lenti-X concentrator (Takara Bio ...
-
bioRxiv - Genetics 2019Quote: ... A single primer (primer IIA from the Clontech SMARTer kit 5’ AAG CAG TGG TAT CAA CGC AGA GTA C 3’ ...
-
bioRxiv - Genomics 2024Quote: SMART-Seq Single Cell PLUS kit (Takara Bio) was used for cDNA library construction ...
-
bioRxiv - Neuroscience 2020Quote: ... supplemented with 0.04% Bovine Serum Albumin and prepared for single-cell separation and labeling using the iCELL8 Single-Cell System (Wafergen, Takara Bio) according to the manufacturer’s recommendations ...
-
bioRxiv - Genetics 2021Quote: ... minimal SD base media (Takara Bio, 630411), and a composition of every essential amino acid except tryptophan (SD/-Trp ...
-
bioRxiv - Genetics 2022Quote: ... galactose/raffinose (Takara Bio Minimal SD Bases), or galactose/raffinose with 10μg/ml doxycycline (Fisher Scientific BP26531) ...
-
bioRxiv - Molecular Biology 2023Quote: ... coated with SD-Leu/-trp (Clontech, USA) plate ...
-
bioRxiv - Molecular Biology 2022Quote: ... and DNA Single Index Kit - 12S Set A (Takara) according to manufacturer’s protocol ...
-
bioRxiv - Cell Biology 2023Quote: ... cells with Lenti-X Packaging Single Shot (Takara Bio) with one of the following plasmids pLVX-Tet3G ...
-
bioRxiv - Plant Biology 2019Quote: ... and yeast cells were grown on SD/-LW medium (minimal media double dropouts, SD medium with-Leu/-Trp) according to the manufacturer’s protocol (Clontech) for 3 days ...
-
bioRxiv - Microbiology 2022Quote: ... Transformed yeast cells were cultured in a synthetic-defined (SD) medium without tryptophan (SD/-Trp broth, Takara Bio USA Inc.) and induced in SD/-Trp broth with 2% raffinose as an alternative carbon source by the addition of 1 to 3% galactose ...
-
bioRxiv - Synthetic Biology 2023Quote: ... or SD-Trp Broth (Takara, 630411 and 630413) at 30°C with shaking at 225 rpm.
-
bioRxiv - Plant Biology 2024Quote: ... 0,77g/L SD-URA drop out mix (Clontech), 0.67 g/L Yeast Nitrogen Base (Sigma-Aldrich) ...
-
bioRxiv - Cell Biology 2023Quote: ... gel-purified (NucleoSpin Gel and PCR Clean-up kit from Takara) and the purified fragment was stored at -20°C ...
-
bioRxiv - Cancer Biology 2021Quote: ... using Takara’s Lenti-X (VSV-G) Packing Single Shots (Takara Bio/ Clontech Laboratories ...
-
bioRxiv - Cancer Biology 2021Quote: ... Samples processed on the ICELL8 Single Cell System (Takara Bio) were prepared using the SMARTer ICELL8 3 ‘DE Reagent Kit V2 (Takara Bio ...
-
bioRxiv - Physiology 2021Quote: ... The SMART-Seq Single Cell Kit (SSsc; Cat. # 634472, Takara) was used and reaction volumes were miniaturized 6 times with the aid of the Mosquito HV robot as per the kit provider User Guide (cDNA synthesis using the mosquito HV genomics with the SMART-Seq® Single Cell Kit ...
-
bioRxiv - Cancer Biology 2022Quote: ... Lentivirus was produced with LentiX Packaging Single Shots (Clontech, 631278) according to the manufacturer’s protocol and was used to transduce MG63.3-GFP-Cas9i cells at an MOI of ∼0.3 ...
-
bioRxiv - Bioengineering 2023Quote: Single colonies were grown in 10 ml YPDA media (Takara) in 50 ml tubes for 24h ...
-
bioRxiv - Plant Biology 2020Quote: ... 3-μL aliquots of each dilution were used to inoculate SD/−Trp/−His/−Ade medium and SD/−Trp/−His medium with X-α-Gal (Clontech). The inoculated media were incubated for 4 days at 30 °C ...