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Citations for Takara Bio :
151 - 200 of 6460 citations for Mouse Starch Binding Domain Containing Protein 1 STBD1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... His-tagged NTD domain constructs were purified from clarified supernatants using 2 ml of cobalt resin (Takara Bio TALON), washing with 50 column volumes of 20 mM HEPES-HCl pH 8.0 and 150 mM NaCl and eluted with 600 mM imidazole ...
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bioRxiv - Microbiology 2020Quote: ... ramulus DSM 15684 were cloned without signal peptide or transmembrane domain from genomic DNA using In-Fusion cloning (Takara) and the primers in Table S8 into the EcoRI and Ncol restriction sites of the corresponding plasmids ...
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bioRxiv - Plant Biology 2023Quote: ... fused with GAL4 activation domain was amplified and subcloned into the prey vector pGADT7 (Clontech Laboratories, Inc., Kusatsu, Japan). Competent yeast cells were prepared according to the Clontech Yeast protocols handbook using the Y1H Gold yeast strain ...
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bioRxiv - Physiology 2024Quote: ... (Cx43: 1:3000 custom-produced rabbit polyclonal; eGFP: 1:1000 mouse monoclonal (Clontech Laboratories Inc ...
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bioRxiv - Immunology 2021Quote: ... containing 2 μl lysis buffer per well (1:20 RNase inhibitor (Clontech) in 0.2% (v/v ...
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bioRxiv - Cell Biology 2023Quote: ... pH 7.4) and the protein contents of the cell lysate were quantified using a BCA Protein Assay Kit (TAKARA, T9300A) following the manufacturer’s protocol (Song et al. ...
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bioRxiv - Cell Biology 2023Quote: ... Supernatants were collected and mixed with Laemmli buffer after measuring the protein concentration using a TaKaRa BCA Protein Assay Kit (TaKaRa). Samples were subjected to SDS-polyacrylamide gel electrophoresis and electrotransferred onto polyvinylidene difluoride membranes ...
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bioRxiv - Neuroscience 2021Quote: ... Three technical replicates of 300 cells each were sorted from each individual mouse into 1.5mL microcentrifuge tubes containing cell lysis buffer buffer from the Clontech SMART-Seq HT (Takara) kit for direct cDNA synthesis and RNAseq library generation.
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bioRxiv - Molecular Biology 2024Quote: ... 4.9 μg DNA was transfected per dish containing a 1:1 molar ratio of pmCherry-N1 (Clontech) and of the pUC18-GFP or linear GFP substrate ...
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bioRxiv - Neuroscience 2023Quote: ... Green Fluorescent Protein (GFP) antibody (JL-8, 1:500, Clontech), Red Fluorescent Protein Antibody (DsRed ...
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bioRxiv - Molecular Biology 2019Quote: A DNA fragment containing CDS of each Hero protein and C-terminal FLAG and His tags was inserted into pCold I (Takara) by NEBuilder HiFi DNA Assembly Master Mix (NEB) ...
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bioRxiv - Immunology 2022Quote: ... The SMARTer Mouse TCR a/b Profiling Kit (Takara, Cat. No. 634403) was used to amplify both TCRα and TCRβ sequences ...
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bioRxiv - Biophysics 2020Quote: Orai1-CAD-CAD-GFP constructs were designed by cloning Orai1 and STIM1-CAD domains (aa344-449) into peYFP-N1 (Clontech). Linker regions between Orai1 and CAD (Orai1-linker-1-CAD-linker-2-CAD-YFP ...
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bioRxiv - Cell Biology 2022Quote: ... the PCDH15-CD2 isoform cytoplasmic domain was cloned using the HindIII and BamHI restriction sites of the pmCherry-C2 vector (Clontech), resulting in soluble protein tagged with cherry at its N-terminus.
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bioRxiv - Microbiology 2020Quote: ... cDNA library derived from soybean hypocotyl and roots RNA were constructed to pGADT7 plasmid fused with GAL4 activation domain (AD) by Clontech company ...
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bioRxiv - Plant Biology 2022Quote: ... GID1b and GID1c cDNAs inserted into pDONR221 were fused to the activation domain GAL4 (AD) after recombination into pGADT7 (Clontech).
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bioRxiv - Microbiology 2021Quote: ... Forward and reverse primers carrying the above restriction sites were designed to amplify a 3kb fragment from Diaph2 MGC human cDNA clone (Dharmacon) and the amplicon encompassing the entire cDNA sequence minus the DAD domain was cloned into pLVX-mCherry-N1 (Clontech) to generate lentiviral expression vector pLVXdeltaDADmcherry ...
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bioRxiv - Plant Biology 2020Quote: The transformation of each plasmid for the respective domain configurations was performed as described by Gietz and Schiestl (2007) using Saccharomyces cerevisiae strain HF7C (Clontech).
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bioRxiv - Plant Biology 2021Quote: ... fragments containing a stop codon were recombined into Gateway-compatible versions of the GAL4 DNA BD vector pGBT-9 and the activation domain vector pGAD424 (Clontech) using L/R-clonase (ThermoFisher) ...
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bioRxiv - Genetics 2019Quote: ... The entire open reading frame for the protein Reg1 and its derivative Reg1-P231L was expressed as a fusion to the Gal4 activation domain in the plasmid pACT2 (Clontech).
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bioRxiv - Microbiology 2023Quote: ... Fragments encoding the CD45 extracellular domain (ECD) and transmembrane region were amplified by PCR and cloned into pEGFP-N1 (Clontech) using the EcoRI and BamHI restriction sites ...
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bioRxiv - Immunology 2022Quote: ... Mate & Plate™ cDNA Library in a pGADT7 vector with the GAL4 DNA-activation domain (AD) and pre-transformed into yeast strain Y187 was acquired from Takara Bio ...
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bioRxiv - Plant Biology 2023Quote: ... The LexA fusion constructs (bait) and the activation domain fusion constructs (prey) were co-transformed into yeast strain EGY48[p8op-lacZ] (CLONTECH). Clones containing both constructs were selected on medium lacking His ...
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bioRxiv - Plant Biology 2023Quote: ... pGADT7 plasmids encoding the effector domain of different ZiF were co-transformed into chemically competent Y2HGold cells (Takara Bio, USA) with a pGBKT7 plasmid encoding OsExo70F3 in using a Frozen-EZ Yeast Transformation Kit (Zymo research).
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bioRxiv - Plant Biology 2024Quote: ... the full-length ORF of PpGL2 was fused with the GAL4 transcriptional activation domain in the pGADT7 vector (Clontech, Japan), and then co-transformed with the pHIS2 cis reporter vector (containing triple tandem CAAATTG repeat sequence ...
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bioRxiv - Molecular Biology 2024Quote: The RBD-DsRed plasmid was created by PCR cloning the HB domain of RNAse H1 into the pDsRed-Express-C1 vector (Clontech), following the previously described method [32].
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bioRxiv - Genetics 2019Quote: 3000 quiescent satellite cells were lysed after FACS by sorting directly into a 0.2ml tube containing 1 μl SMART-Seq Reaction Buffer (95% SMART-Seq 10x lysis buffer containing 5% SMART-Seq RNAse Inhibitor, Takara Bioscience, Cat. 634890) in 8μl ddH20 ...
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bioRxiv - Cell Biology 2019Quote: Expression plasmid for mouse FLAG tagged BAG3 (pFLAG-BAG3) was constructed by cloning partial mouse BAG3 cDNA containing the whole CDS into pEGFP-N1 (Clontech). Forward primer sequence used to clone BAG3 plasmid is 5′-AAAGGATCCAGCGCCGCCACCC-3′ and the reverse primer sequence is 5′-GACTCTAGATCACTAGGGAGCCACCAGGTTGC-3′ ...
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bioRxiv - Neuroscience 2020Quote: ... Primary antibodies used were mouse anti-STEM121 (1:500; Takara), chicken anti-GFAP (1:2,000 ...
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bioRxiv - Cell Biology 2021Quote: ... mouse anti-GFP (Clontech, JL-8, 1:5000 for western), rabbit anti-GFP (Chromotek ...
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bioRxiv - Cell Biology 2022Quote: ... mouse GFP antibody (Clontech, clone JL8, used at 1:4,000) was obtained from Takara (Saint-Germain-en-Laye ...
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bioRxiv - Neuroscience 2022Quote: ... a mouse anti-mCherry primary antibody (Takara Bio, 1:5000) was used instead of the substance P primary antibody to visualize hM3Dq-mCherry or mCherry-expressing cells.
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bioRxiv - Developmental Biology 2022Quote: ... mouse anti-mCherry (1:200, Takara Bio/Clontech Laboratories, 632543), rat anti-BrdU (1:250 ...
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bioRxiv - Cell Biology 2020Quote: Mouse GFP antibody (Clontech, clone JL8, used at 1:4,000) was obtained from Takara (Saint-Germain-en-Laye ...
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bioRxiv - Cell Biology 2021Quote: ... mouse anti-GFP (Clontech, JL-8, 1:5000 for western), mouse anti-Dhc (Developmental Studies Hybridoma Bank ...
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bioRxiv - Developmental Biology 2022Quote: ... mouse anti-mCherry (1:200, Takara Bio/Clontech Laboratories, 632543), rat anti-BrdU (1:250 ...
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bioRxiv - Microbiology 2023Quote: ... Immunoblotting was performed using mouse anti-eGFP (Takara, 1:2000), rabbit anti-Actin (Bethyl ...
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bioRxiv - Biochemistry 2021Quote: ... The recombinant proteins were purified with His60 Ni Gravity Column Purification Kit (Clontech) following a manufacturer’s protocol ...
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bioRxiv - Plant Biology 2020Quote: ... GFP-TSN-interacting proteins and native TSN were detected by mouse α -GFP (monoclonal antibody JL-8; Clontech) and rabbit α-TSN antibodies at final dilutions of 1:1,000 and 1:5,000 ...
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bioRxiv - Genetics 2022Quote: ... Total protein was harvested after 3 days of culture and analyzed by Western blot (mouse anti-DsRed, Clontech; rabbit anti-POU6F2 ...
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bioRxiv - Cell Biology 2020Quote: The cDNA coding sequences of the first and second cytoplasmic loop domain of human TMEM39A were cloned into the pGBKT7 vector and screened against a normalized universal human cDNA library (Clontech, 630481), following instruction of the Matchmaker® Gold Yeast Two-Hybrid System (Clontech ...
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bioRxiv - Plant Biology 2020Quote: ... and pGBKT7 (DNA-binding domain vector) by either restriction enzyme-based cloning or In-Fusion HD Cloning method (638910In-Fusion® HD Cloning Plus Takara). Primer sequences used to generate Y2H and Y3H constructs are listed in Supplemental Table 4 ...
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bioRxiv - Genetics 2020Quote: The cDNA coding sequence of the C-terminal domain of human TMEM132D was cloned into the pGBKT7 vector and screened with a normalized universal human cDNA library (Clontech, 630481) in pGADT7 Vector ...
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bioRxiv - Biochemistry 2022Quote: ... in the bait vector pGBKT7 or were fused to the GAL4 activation domain (AD) in the prey vector pGADT7 (Clontech Laboratories). The yeast strain Y2H Gold (Clontech Laboratories ...
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bioRxiv - Cell Biology 2020Quote: ... mouse anti-GFP (for immunohistochemistry 1:1000, Molecular Probes; for Western blotting: 1:10000, Clontech), mouse anti-Tubulin (1:80 ...
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bioRxiv - Systems Biology 2022Quote: ... rabbit dsRed (1:500, Clonetech Takara 632496 to detect Tomato protein); mouse βTubulin (1:600 ...
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bioRxiv - Molecular Biology 2020Quote: ... containing the beta-globin (HBB) 5’-UTR using the In-Fusion® HD Cloning Kit (Takara). The subsequent mutations in the TCRA and TCRB 3’-UTRs were generated using these initial constructs ...
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bioRxiv - Immunology 2019Quote: ... The cDNA was generated and amplified by SMARTer Mouse TCRαβ Profiling Kit (Clontech). Libraries were sequenced by illumina sequencer MiSeq (2×250 cycles) ...
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bioRxiv - Neuroscience 2023Quote: ... 48-52 mCherry-positive cells from each mouse were sorted into 8-well strips containing SMART-Seq lysis buffer with RNase inhibitor (0.17 U/µL; Takara Cat#ST0764). For retrograde transsynaptic tracing ...
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bioRxiv - Developmental Biology 2023Quote: ... Only 0.5 µL of media containing cumulus cells from each mouse was added to 10 µL lysis buffer (Takara Bio Inc.). The oocytes were then treated with 1 mg/mL collagenase to remove the ZP ...