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Citations for Takara Bio :
601 - 650 of 6565 citations for Mouse Direct PCR Kit For Genotyping since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2020Quote: ... The products obtained were subsequently subjected to semi-nested PCR with a LA Taq Hot Start polymerase kit (TaKaRa Biotechnology). The first round of amplification was performed with primers ...
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bioRxiv - Developmental Biology 2022Quote: ... first-strand cDNA synthesis and subsequent cDNA amplification by 22 cycles of polymerase chain reaction (PCR) were performed using the SMARTer stranded RNA-seq kit (Takara). The synthesized first-strand cDNA and amplified cDNA were purified using Ampure XP Beads (Beckman Coulter) ...
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bioRxiv - Biochemistry 2022Quote: A DNA fragment coding HA-tagged Dcp2 was amplified by PCR and cloned into pIExZ vector (Izumi et al, 2020) by In-fusion cloning kit (Takara).
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bioRxiv - Biochemistry 2022Quote: cDNA fragment of Gtsf1 was amplified by RT-PCR from BmN4 total RNAs and cloned into pIExZ vector (Izumi et al, 2020) by In-fusion cloning kit (Takara).
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bioRxiv - Biochemistry 2022Quote: A cDNA fragment of Gtsf1L was amplified by RT-PCR from BmN4 total RNAs and cloned into pIZ vector by In-fusion cloning kit (Takara).
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bioRxiv - Evolutionary Biology 2022Quote: ... The cDNA samples were cloned into a pUC19 vector (PCR-amplified with primers 11 and 12) using In-Fusion HD Cloning Kit (Takara). After transformation into Escherichia coli ...
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bioRxiv - Molecular Biology 2023Quote: ... The ORF of zebrafish znf598 (ENSDARG00000014945) was amplified by RT-PCR and cloned into pCS2+ via XhoI/XbaI restriction sites using DNA Ligation Kit (TAKARA). To generate znf598 point mutation in ORF (znf598 C13/16A) ...
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bioRxiv - Molecular Biology 2023Quote: ... The ORF of zebrafish rpl36 (ENSDARG00000100588) was amplified by RT-PCR and cloned into pCS2+ via EcoRI/XhoI restriction sites using DNA Ligation Kit (TAKARA). The ORF of zebrafish znf598 (ENSDARG00000014945 ...
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bioRxiv - Neuroscience 2022Quote: ... the BamHI/BsrGI digested PCR product was ligated to the linearized backbone using the DNA Ligation Kit - Mighty Mix (TaKaRa) resulting pPB-ef1a-NLS-EGFP-T2A-puro.
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bioRxiv - Molecular Biology 2022Quote: ... Probes for FLuc (500 bp) were generated using gel-purified PCR amplicons containing GFP sequence and a BcaBEST Labeling kit (Takara) and [α-32P]-dCTP (PerkinElmer) ...
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bioRxiv - Microbiology 2023Quote: ... The resultant reaction mixture was then 10-fold diluted and subjected to quantitative PCR using TB Green Premix Ex Taq II kit (TaKaRa) in combination with a tag primer (no ...
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bioRxiv - Cancer Biology 2023Quote: ... the agarose gel pieces were cut out and digested using NucleoSpin® Gel and PCR Clean-Up kit (Takara, 740609), according to the manufacturer’s protocols ...
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bioRxiv - Cell Biology 2023Quote: ... the RNA was reverse transcribed into complementary DNA by PrimeScript RT (reverse transcriptase) using a primeScript RT-PCR kit (Takara), and qPCR was performed with SYBR Green-Master Mix (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... Titration of the concentrated viruses was performed by AAVpro Titration Kit (for Real Time PCR) Ver.2 (Takara Bio Inc) following the manufacture’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... generated cDNA was subjected to real-time PCR analysis using SYBR® Premix Ex Taq™ II kit (TAKARA BIO) with the sets of specific primers (Table 1) ...
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bioRxiv - Cancer Biology 2023Quote: ... The number of virus copies/ml was determined using the Lenti-X™ qRT-PCR Titration Kit (Takara Bioscience, 631235) according to the manual ...
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bioRxiv - Genetics 2023Quote: ... Reverse transcription was performed using the oligo (dT) primer and AMV reverse transcriptase contained in the TaKaRa RNA PCR kit (TaKaRa). The RT-PCR was performed using KOD One PCR Master Mix (TOYOBO ...
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bioRxiv - Cell Biology 2023Quote: cfDNA in the conditioned cell culture medium was extracted using NucleoSpin Gel and PCR Clean-up kit (Takara, Duren, Germany), and quantified with Nanodrop (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: A PCR product containing the Human ORF for DDX3X was obtained directly from RNA using the Primescript High Fidelity RT-PCR kit from Takara. The PCR primers introduced BamHI and NotI sites at the 5’ and 3’ ends respectively ...
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bioRxiv - Immunology 2023Quote: ... Each gene fragment was PCR-amplified and cloned into a pcDNA 3.1 vector by using the In-Fusion HD cloning kit (Takara Bio). Lipofectamine 2000 (Invitrogen ...
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bioRxiv - Microbiology 2024Quote: ... The RNA samples were then reverse transcribed employing a One-Step SYBR PrimerScript reverse transcription (RT)-PCR kit (TaKaRa, Japan). Nested PCR conditions were as follows ...
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bioRxiv - Neuroscience 2024Quote: ... and then the tip of the recording electrode was broken into the nested PCR reagent system (Prime ScriptTm RT reagent Kit with QDNA Eraser, Cat: RR0471, Takara), and a cDNA synthesis kit was used to synthesize cDNA following the manufacturer’s protocol (PrimeScriptm II1st Strand cDNA Synthesis Kit) ...
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bioRxiv - Genomics 2023Quote: ... Digests were run on a 0.8% agarose-TAE gel and full-length plasmids were extracted with the Nucleospin PCR and Gel Cleanup Kit (Takara, 740609). A LR reaction was performed with 150 ng entry library and 150 ng pDEST_HC_Rec_Bxb_v2 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... PCR amplification was carried out using EmeraldAmp GT PCR Master Mix (Clontech, Takara) according to the manufacturer’s protocol and the primers shown in Table S1 and Figure 2 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... PCR amplification was carried out using EmeraldAmp GT PCR Master Mix (Clontech, Takara) according to the manufacturer’s protocol and the primers shown in Table S1 and Figure 2 ...
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bioRxiv - Molecular Biology 2021Quote: ... The PCR product was purified with NucleoSpin Gel and PCR Clean-up (Takara) and digested with AsiSI at 37 °C overnight ...
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bioRxiv - Microbiology 2021Quote: ... pneumophila (RefSeq NC_002942.5) genomic DNA by PCR using CloneAmp HiFi PCR premix (Takara) and the relevant primers ...
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bioRxiv - Systems Biology 2021Quote: ... all PCRs utilized a high fidelity polymerase (CloneAmp HiFi PCR Premix, Takara 639298). Plasmids contained components of the synthetic inducible system flanked by homology to the intended genomic integration locus and were restricted prior to transformation ...
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bioRxiv - Neuroscience 2023Quote: ... The PCR mixture contains 5 ul EmeraldAmp GT PCR Master Mix (Takara, #RR310B), 1 ul genomic DNA ...
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bioRxiv - Genomics 2022Quote: We performed PCR with CloneAmp HiFi PCR Mix (Takara Biosciences, Mountain View, CA) and added DMSO to a final concentration of 5-10% (v/v ...
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bioRxiv - Plant Biology 2023Quote: ... RT-PCR was performed using SapphireAmp® Fast PCR Master Mix (Takara; RR350A), while iTaq™ Universal SYBR® Green Supermix (Biorad ...
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bioRxiv - Neuroscience 2023Quote: PCR amplifications for cloning were performed with the CloneAmp HiFi PCR Premix (Clontech) and plasmid fusion/reassembly were performed with the NEBuilder HiFi DNA Assembly Master Mix (NEB) ...
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bioRxiv - Cell Biology 2024Quote: ... semiquantitative PCR was performed using 2X EmeraldAmp GT PCR Master Mix (Takara-#RR310) and gene-specific primers and the amplified PCR products were visualized on 2% agarose gel ...
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bioRxiv - Neuroscience 2021Quote: ... MYC (mouse, Clontech, 631206 ...
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bioRxiv - Bioengineering 2022Quote: ... PCR was performed in the TaKaRa PCR Thermal Cycler Dice Touch (TaKaRa, Shiga, Japan) using the Tks Gflex DNA Polymerase (TaKaRa) ...
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bioRxiv - Molecular Biology 2021Quote: ... PCR was conducted using a Takara PCR thermal cycler Dice mini (Takara Bio Inc.) as follows ...
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bioRxiv - Cancer Biology 2020Quote: ... PCR products were purified with NucleoSpin Gel and PCR Clean-Up (Takara Bio, #740609). 400 ng of PCR products mixed with NEB buffer 2 were denatured at 95°C for 5 minutes and ramped down to 25°C ...
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bioRxiv - Plant Biology 2021Quote: ... Quantitative PCR experiments were performed using SYBR Green PCR Master Mix (Takara, Otsu, Japan) with Bio-Rad CFX96 real-time PCR machine ...
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bioRxiv - Molecular Biology 2020Quote: ... Primary PCR was performed using EmeraldAmp® GT PCR Master Mix (Takara cat# RR310A) 10 ul ...
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bioRxiv - Microbiology 2020Quote: ... Each PCR reaction contained 2.5 µl of PCR buffer (TaKaRa, 10x concentration, 1x final), 1 µl of the Golay barcode tagged forward primer (10 µM concentration ...
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bioRxiv - Molecular Biology 2022Quote: ... PCR products were purified using NucleoSpin Gel and PCR Clean-up (TaKara Bio Inc.), and then analyzed and quantified by Nanodrop spectrometer (Thermo Fisher Scientific ...
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bioRxiv - Zoology 2023Quote: ... The PCR products were purified using Nucleospin Gel and PCR Clean-up (Takara Bio). Nucleotide sequence analysis was outsourced from a commercial sequencing service (Eurofins ...
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bioRxiv - Biochemistry 2023Quote: ... qRT-PCR was carried out using SYBR Premi qRT-PCR ExTaq™ II (Takara) and analyzed on a Bio-Rad CFX96 real time PCR cycler (BioRad ...
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bioRxiv - Cell Biology 2023Quote: ... Clonal lines were genotyped by PCR using EmeraldAmp GT PCR Master Mix (Takara, RR310A) and target specific primers flanking the Myrlin transcription start site ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Each PCR reaction consisted of 25μL PrimeSTAR Max PCR mix (Takara Kusatsu, Shiga, Japan), 2 μM of each primer ...
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bioRxiv - Molecular Biology 2024Quote: The quantitative RT-PCR (qRT-PCR) was performed with SYBRGreen II (Takara, Dalian, China). The 7500 system (Applied Biosystems ...
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bioRxiv - Cell Biology 2024Quote: ... PCR products were purified using NucleoSpin Gel and PCR Clean-up (TaKara Bio Inc.), and then analyzed and quantified by Nanodrop spectrometer (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... and quantitative real-time PCR was performed using the SYBR® Premix Ex Taq II Reagent Kit (Takara, Cat. Cat. #RR820A) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... PCR amplified fragments and linearized vector by restriction enzyme digestion were assembled using In-Fusion HD Cloning Kit (Takara Bio Inc.). pEGFPN3-ARL4C was constructed as previously described(Matsumoto et al ...
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bioRxiv - Developmental Biology 2021Quote: ... three gene-specific reverse primers were designed and a nested PCR program was used according to the protocol of a genome walking kit (Takara, Japan). The sequences of the primers used for amplification are shown in Table S1 ...