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Citations for Takara Bio :
151 - 200 of 893 citations for M CHERRY MRNA mCherry Fluorescent Protein coding mRNA since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... pEF1a-mCherry (Clontech), and pCLIPf (NEB) ...
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bioRxiv - Cell Biology 2022Quote: ... mCherry-C1 (Clontech). The 229E Spike cDNA was amplified by PCR with the forward primer ...
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bioRxiv - Molecular Biology 2021Quote: ... Expression of these mRNAs was assayed using TB Green™ Premix Ex Taq™ II (Tli RNase H Plus; Takara) according to manufacturer’s instructions with QuantiTect primer assays (Qiagen) ...
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bioRxiv - Molecular Biology 2019Quote: ... followed by reverse transcription of the enriched mRNA into cDNA using Clontech SMARTer PCR cDNA Synthesis Kit (Takara, Shiga, Japan). PCR cycle optimization was used to determine the optimal amplification cycle number for the downstream large-scale PCR reactions ...
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bioRxiv - Microbiology 2019Quote: ... The IL-1β and components of RISC mRNA expression levels of the NA cells were quantified with the SYBR Green qPCR kit (Takara) by following the manufacturer’s instruction using gene-specific primers (S2 Table) ...
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bioRxiv - Cancer Biology 2019Quote: ... Amplification and detection of mRNA were conducted by using the Thermal Cycler Dice® Real Time System (Takara Bio, TP800), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Double stranded cDNA was prepared from 1 μg of each mRNA sample using the PrimeScript Double Strand cDNA Synthesis Kit (Takara) and a cDNA library was established with Ligation Sequencing Kit (SQK-LSK109 ...
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bioRxiv - Developmental Biology 2023Quote: ... The yeast two-hybrid library was generated from the poly(A)+ mRNA isolated from mixed-stage N2 nematodes using the Matchmaker kit (Clontech) as per the manufacturer’s protocol and transformed into Y1HGold strain (MATα ...
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bioRxiv - Developmental Biology 2023Quote: ... and RT-PCR was performed to detect sorcs2 mRNA in wt embryos using the Titanium One-Step RT-PCR kit (Clontech) with primers 5’-TTTTGCGCACCTGTACCCAGCTG-3’ and 5’-TAACGCGCTCCTGAAGCAGAGTC-3’ for detection of mRNA after injection of different amounts of sMO and 5’-TTTTGCGCACCTGTACCCAGCTGTGTT-3’ and 5’ TAACGCGCTCCTGAAGCAGAGTCCATT-3’ for the different developmental stages ...
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bioRxiv - Systems Biology 2021Quote: ... the inducible fluorescent protein was induced by adding 1 μg/ml doxycycline (Clontech) alone or together with 100 nM rapamycin (Harveybio ...
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bioRxiv - Neuroscience 2021Quote: ... the fluorescent proteins were amplified from pAM FLEX eGFP and pmCherry-C1 (Clontech) respectively ...
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bioRxiv - Neuroscience 2023Quote: ... the fluorescent proteins were amplified from pAM FLEX eGFP and pmCherry-C1 (Clontech) respectively ...
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bioRxiv - Developmental Biology 2020Quote: ... mCherry cDNA was amplified using primers NheI mCherry Fw (5’-acgctagctatggtgagcaagggcgaggag-3’) and XhoI mCherry Rv (5’-gactcgagttacttgtacagctcgtccat-3’) from mCherry Vector (Clontech), and then the product was introduced into NheI-XhoI sites of the pFRT-SV40-FRT vector (Gift from Elizabeth R ...
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bioRxiv - Cell Biology 2019Quote: pEGFP-N1 (EGFP) and mCherry-C1 (mCherry) plasmids were purchased from Clontech Laboratories ...
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bioRxiv - Plant Biology 2022Quote: We amplified the coding sequences of MpSETA and MpICE2 from cDNA derived from mRNA of Tak-1 thalli by PCR using PrimeSTAR Max DNA polymerase or PrimeSTAR GXL polymerase (Takara Bio). Also ...
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bioRxiv - Neuroscience 2021Quote: ... reverse transcription and cDNA amplification were performed on the Fluidigm C1 machine according to the manufacturer’s mRNA-seq protocol using the SMARTer Ultra Low RNA Kit for the Fluidigm C1 System (Takara Bio #634833). cDNA amplicons (∼3 µL ...
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bioRxiv - Neuroscience 2021Quote: mRNA was purified and the cDNA was synthesized with the mRNA fragments as templates using SMART-Seq V4 RNA kit (Clontech # 634889). The sequencing libraries were generated using Nextera XT DNA Library Preparation Kit (Illumina #FC-131-1024) ...
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bioRxiv - Immunology 2022Quote: Illumina Compatible low input mRNA libraries were prepared using the Smart-Seq V4 Ultra Low Input RNA kit (Takara Bio, USA) and KAPA HyperPlus Library Preparation kit (Roche) ...
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bioRxiv - Cancer Biology 2022Quote: ... The expression of mRNA was examined by real-time quantitative-PCR (qRT-PCR) using SYBR Green PCR Master Mix (Takara, Japan) and a LightCycler480 Detection System (Roche ...
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bioRxiv - Molecular Biology 2023Quote: ... The single isoform of DCR1 was amplified by RT-PCR from mRNA isolated from S2 cells and reverse transcribed using the Oligo(dT)-primed RNA to cDNA EcoDry premix (Takara Bio). The CDS for AGO1 and GW182 were PCR amplified from pAFW-Ago1 (Addgene #50553 ...
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bioRxiv - Immunology 2023Quote: ... Reverse transcription was performed on 1 µg total mRNA per sample using PrimeScript™ RT Reagent Kit with gDNA Eraser (Takara) according to the manufacturer’s instruction ...
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bioRxiv - Cell Biology 2019Quote: ... mCherry-N1 (Clontech #632523); EGFP-N1 (Clontech #6085-1) ...
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bioRxiv - Cell Biology 2021Quote: ... pLVX-mCherry-N1 (Clontech), pCDH-EGFP-TRAIP (15) ...
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bioRxiv - Neuroscience 2022Quote: ... mouse anti-mCherry (Takara Bio ...
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bioRxiv - Biophysics 2019Quote: ... 300 ng of the plasmid encoding the green fluorescent protein (pEGFP, Clontech, CA, USA) and 12 μL of Lipofectamine/Plus reagent ...
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bioRxiv - Biochemistry 2021Quote: ... monoclonal antibody for green fluorescent protein (JL-8; Takara Bio, Kusatsu, Japan; recognizes VC), rabbit anti-GFP (D5.1 Cell Signaling Technology 2956S ...
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bioRxiv - Molecular Biology 2019Quote: ... The AT1AR sequence was cloned into pN1-mCherry and pN1-p2A-mCherry (Clontech) using HindIII and AgeI sites ...
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Enterohepatic Transcription Factor CREB3L3 Protects Atherosclerosis via SREBP Competitive InhibitionbioRxiv - Physiology 2020Quote: ... mCherry-tagged human SREBP-1c was inserted into pmCherry (mCherry-SREBP-1c) (Clontech), and HA-tagged hamster SCAP ...
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bioRxiv - Microbiology 2023Quote: ... His-tagged mCherry (mCherry-His) was PCR amplified from pmCherry-C1 vector (Takara) templates with primers encoding a His-tag and NcoI/NotI cut sites (Takara) ...
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bioRxiv - Bioengineering 2024Quote: ... lentivirus targeting mCherry to the cell membrane (Takara, 0026VCT, rLV.EF1.mCherry-Mem-9) was used according to manufactures recommendation ...
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bioRxiv - Animal Behavior and Cognition 2019Quote: ... Tissues expressing mCherry-tagged Yellow protein (ymCherry) were stained with rabbit anti-dsRed (Clontech 632496, 1:1000) and rat anti-DN-Cadherin (DN-Ex #8 ...
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bioRxiv - Cancer Biology 2024Quote: ... Eukaryotic expression vectors encoding green fluorescent protein (GFP)-tagged proteins were generated by inserting PCR-amplified fragments into pd1-EGFP-N1 vector (6073-1, Clontech). Prokaryotic plasmids encoding GST-fusion proteins were constructed using pGEX-4T-1 bacterial expression vector (27-4580-01 ...
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bioRxiv - Neuroscience 2021Quote: Total mRNA was extracted from the hypothalamus using a TRIzol reagent according to the manufacturer’s instructions (Takara Biotechnology Co. Ltd, Dalian, China). RNA was reverse transcribed into cDNA using a cDNA reverse transcription kit (Catalog no ...
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The pan-cancer lncRNA PLANE regulates an alternative splicing program to promote cancer pathogenesisbioRxiv - Cancer Biology 2020Quote: ... antisense PLANE and NCOR2 pre-mRNA were generated by PCR amplification from cDNAs using PrimerSTAR Max DNA Polymerase (TAKARA, #R045A; Dalian, China). Forward primers containing the T7 RNA polymerase promoter sequence and reverse primers without the promoter sequence were used for synthesizing PLANE ...
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bioRxiv - Molecular Biology 2023Quote: ... Accurate quantification of mRNA levels was meticulously conducted by employing the GAPDH reference gene and utilizing the cutting-edge SYBR Green kit (Takara, Kyoto, Japan) on the state-of-the-art Real-time PCR Detection System manufactured by Bio-Rad ...
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bioRxiv - Microbiology 2023Quote: ... The cells were cultured again overnight and subjected to the quantification of mRNA by qRT-PCR using the CellAmp Direct RNA Prep Kit for RT-PCR (Real Time) (3732, Takara Bio Inc.), One Step TB Green PrimeScript PLUS RT-PCR Kit (Perfect Real Time ...
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bioRxiv - Bioengineering 2023Quote: GA-MatryoshCaMP6s was constructed by replacement of the coding sequence of LSSmOrange in GO-MatryoshCaMP6s with the LSSmApple coding sequence by In-Fusion cloning (Takara) following manufacturer recommendations ...
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bioRxiv - Microbiology 2022Quote: ... the coding sequence was cloned by InFusion (Clontech) in the plasmid pMT85 under the control of the promoter PTufA (42) ...
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bioRxiv - Microbiology 2022Quote: ... or the mEos3.2 coding sequence using InFusion (Clontech). Plasmids carrying the cassettes were checked by Sanger sequencing (Genewiz) ...
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bioRxiv - Bioengineering 2023Quote: ... The coding sequences for ZsGreen (pIRES2-ZsGreen1, Takara) and Luciferase (luc2 ...
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bioRxiv - Microbiology 2021Quote: ... These fragments were cloned at the XmnI site of pPsfiAcherry and pPsfiA*cherry respectively using the In-Fusion HD cloning kit (Clontech). All constructs were verified by PCR and sequencing ...
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bioRxiv - Cancer Biology 2021Quote: ... plasmid or the enhanced green fluorescent protein (EGFP) plasmid (pEGFP-C1, Clontech/Takara Bio Europe). Transfections were achieved using Lipofectamine 2000 (Invitrogen ...
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bioRxiv - Cancer Biology 2021Quote: ... plasmid or the enhanced green fluorescent protein (EGFP) plasmid (pEGFP-C1, Clontech/Takara Bio Europe). Transfections were achieved using Lipofectamine 2000 (Invitrogen ...
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bioRxiv - Physiology 2020Quote: ... transfections also contained 0.2 μg of the pEGFP plasmid (Enhanced Green Fluorescent Protein plasmid, Clontech), and EGFP expression served as a marker of transfection ...
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bioRxiv - Cell Biology 2019Quote: ... and anti-mCherry (632543, Clontech)) were diluted in blocking buffer and incubated with the membranes overnight at 4 °C ...
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bioRxiv - Neuroscience 2021Quote: ... and mCherry (1:200, Takara Bio USA Inc./Clontech Laboratories ...
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bioRxiv - Plant Biology 2022Quote: ... or anti-mCherry (632543, Takara) antibodies at dilutions of 1:3000 and 1:2000 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... mouse anti-mCherry (Clontech, 632543) 1:200 ...
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bioRxiv - Neuroscience 2021Quote: ... mCherry (rabbit-anti-dsRed; Takara Bio ...
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Mask, the Drosophila Ankyrin Repeat and KH domain-containing protein, regulates microtubule dynamicsbioRxiv - Neuroscience 2021Quote: ... rabbit anti-mCherry (632496, Clontech) at 1:1000 ...