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Citations for Takara Bio :
1 - 50 of 893 citations for M CHERRY MRNA mCherry Fluorescent Protein coding mRNA since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... mRNA was prepared using the NucleoTrap mRNA Mini Kit (Clontech) from 500 ng of total RNA ...
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bioRxiv - Cell Biology 2024Quote: ... total mouse liver mRNA (Takara) was used.
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bioRxiv - Cell Biology 2023Quote: ... by replacing EGFP-coding sequences with mCherry-coding sequences using In-Fusion® HD Cloning Kit (Clontech).
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bioRxiv - Developmental Biology 2021Quote: ... The mRNA samples were then processed for cDNA preparation using PrepX mRNA-8 (Takara 640096) protocol ...
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bioRxiv - Neuroscience 2023Quote: ... mRNA amplification and cDNA preparation were performed using the SMARTer mRNA amplification kit (Takara #634940). Labeled samples were sequenced using an Illumina NextSeq 500 sequencer using 75 base pair single read protocols.
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bioRxiv - Cell Biology 2021Quote: ... The mCherry coding region was amplified from the pmCherry-N1 (Clontech) plasmid using mCher-F/mCher-R primers and cloned into the pGT-GFPbsc plasmid at BglII/XhoI sites ...
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bioRxiv - Neuroscience 2022Quote: ... PolyA+ mRNA was extracted from total RNA using Oligotex TM-dT30 mRNA Purification Kit (Takara Bio), according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: 1 μg brain mRNA (Takara Bio) or 10 μg of brain total RNA (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... mRNA was retro-transcribed by Takara kit (Takara Bio) ...
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bioRxiv - Microbiology 2021Quote: CFP/YFP plasmids were constructed by cloning the fluorescent protein coding sequence into pSTV28 (Takara Bio Europe SAS) with EcoRI/SalI restriction sites ...
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bioRxiv - Microbiology 2022Quote: ... The coding sequence of enhanced green fluorescent protein (eGFP) was amplified from plasmid eGFP-C1 (6084-1, Clontech) using primers eGFPN-F/eGFPN-R ...
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bioRxiv - Molecular Biology 2022Quote: ... Distribution of mat mRNAs or mat-exon1 mRNAs in transfected cells was assessed using SBYR-Green (Takara, Japan)-based quantitative PCR analysis on Aligent Mx3005P ...
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bioRxiv - Neuroscience 2023Quote: ... 5) cDNA coding eGFP protein (Clontech). 6 ...
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bioRxiv - Genomics 2022Quote: ... First strand cDNA synthesis was performed on mRNA fragments with SMARTscribe™ M-MLV Reverse Transcriptase (Takara Bio Inc.). Illumina barcode PCR reactions were performed to prepare the cDNA for Illumina 75 bp paired-end sequencing ...
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bioRxiv - Microbiology 2020Quote: ... Japan) and then mRNA was purified from total RNA by Oligotex −dT30 Super mRNA Purification Kit (Takara Bio, Kusatsu, Japan). Double stranded cDNA was prepared from 1 μg of each mRNA sample using the PrimeScript Double Strand cDNA Synthesis Kit (Takara ...
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bioRxiv - Genetics 2022Quote: ... the Oligotex-dt30
mRNA purification kit (TAKARA) was used for poly(A ... -
bioRxiv - Biochemistry 2023Quote: ... Total RNA was further purified using mRNA Dynabeads and the purified mRNA was used as template for synthesis of cDNA using SMARTer PCR cDNA kit (Takara-Bio), with 18 cycles of amplification ...
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bioRxiv - Neuroscience 2019Quote: PLVX-cherry-C1 plasmid was from Clontech and Mtss1L coding region was cloned into the C-terminus of mCherry between BsrG1 and SmaI sites to generate fusion construct of PLVX-cherryC1-Mtss1L (Mtss1L coding region sequence Accession number B) ...
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bioRxiv - Neuroscience 2023Quote: ... Mouse anti-Cherry (1:500; Clontech, 632543), Rabbit anti-Gat (1:4,000 ...
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bioRxiv - Molecular Biology 2022Quote: ... Enrichment of mRNA and library preparation (Nextera XT, Clontech), library quantification (KAPA Library Quantification Kit Illumina ...
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bioRxiv - Cell Biology 2019Quote: The mRNA was extracted using TRIzol (Takara, Shiga, Japan), and concentration was measured using NanoDrop 2000 spectrophotometer (Thermo Scientific) ...
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bioRxiv - Cell Biology 2022Quote: mRNA was captured using poly-T coated dishes (Clontech) and used for library construction using SMARTer technology (SMARTer Stranded RNA-seq Kit ...
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bioRxiv - Cell Biology 2022Quote: ... mRNA was captured using poly-T coated dishes (Clontech) and SMARTer technology was used for library construction (SMARTer Stranded RNA-seq Kit).
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bioRxiv - Molecular Biology 2023Quote: ... mRNA levels were measured using qRT-PCR kit (Takara) in combination with TB Green real-time PCR ...
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bioRxiv - Neuroscience 2023Quote: ... Enrichment of mRNA and library preparation (Nextera XT, Clontech), library quantification (KAPA Library Quantification Kit Illumina ...
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bioRxiv - Developmental Biology 2023Quote: ... the oligo dT-based mRNA isolation kit (Takara Bio) was employed ...
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bioRxiv - Molecular Biology 2023Quote: ... The recombinant AAV2 genome rAAVeCFPrep was constructed by replacing the mCherry coding sequence in the plasmid pAAVtCR (18) with the ECFP coding sequence from pECFP (Clontech, Mountain View, CA, USA). Purified recombinant AAV2 vectors ...
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bioRxiv - Cell Biology 2019Quote: ... The mammalian expression constructs either contained a fluorescent (mCherry; Takara Bio) protein tag or peptide tag (Myc-DDK ...
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bioRxiv - Developmental Biology 2021Quote: ... mRNA was isolated using PrepX PolyA-8 protocol (Takara 640098). The mRNA samples were then processed for cDNA preparation using PrepX mRNA-8 (Takara 640096 ...
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bioRxiv - Developmental Biology 2023Quote: ... cDNA was synthesized by SMART-Seq mRNA kit (Takara, 634772). Library DNAs were prepared according to the Nextera XT DNA Library Preparation Kit (Illumina ...
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bioRxiv - Plant Biology 2024Quote: ... or the SMART-Seq mRNA LP Kit (Takara Bio USA) (for pre-parasitic J2 library generation) ...
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bioRxiv - Biophysics 2019Quote: ... cDNAs encoding cyan fluorescent protein (CFP) and yellow fluorescent protein (YFP) were purchased from Clontech Com ...
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bioRxiv - Developmental Biology 2020Quote: Total mRNA was isolated by using the Nucleospin kit (TaKaRa, 740955) according to manufacturer’s instruction ...
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bioRxiv - Microbiology 2022Quote: ... and the mRNA was then reverse transcribed into cDNA (Takara, Beijing). qRT-PCR was then performed using a Bio-Rad CFX-96 PCR detection system (USA ...
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bioRxiv - Biochemistry 2021Quote: ... Isolated lymphocytes were used for mRNA isolation with RNAsio Plus (TaKara). Reverse transcription was performed using mRNA and a commercial kit (Vazyme Cat ...
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bioRxiv - Bioengineering 2023Quote: ... mRNA of microglia was extracted using RNAiso Plus (#9109, Takara, Japan) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... A cDNA library generated from mouse placenta total mRNA (Takara, 636672) served as template to amplify the domains by PCR ...
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bioRxiv - Neuroscience 2024Quote: ... mRNA library was prepared using the SMART-Seq HT kit (Clontech). Illumina indexes were added to cDNA using the Nextera XT DNA library preparation kit (Illumina) ...
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bioRxiv - Cell Biology 2024Quote: ... Libraries were prepared using the SMART-seq mRNA kit28,38 (Takara Bio) and sequenced on NovaSeq instrument (Illumina ...
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bioRxiv - Bioengineering 2023Quote: ... Plasmids coding EGFP-mutants were generated from EGFP-IRES-mCherry plasmid using TaKaRa MutanBEST Kit (TaKaRa, Kusatsu, Japan). pG5egfp reporter vector was modified from the pG5luc vector (GeneBank Accession Number AF264724 ...
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bioRxiv - Neuroscience 2020Quote: Enhanced green fluorescent protein (EGFP, Clontech), Tag-blue fluorescent protein (Tag-BFP ...
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bioRxiv - Developmental Biology 2022Quote: Total mRNA was isolated using the RNAiso Plus reagent (Takara, Beijing, China) and reverse transcribed using the HiScript II 1st Strand cDNA Synthesis Kit with gDNA wiper (Vazyme ...
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bioRxiv - Molecular Biology 2019Quote: ... Total mRNA was extracted using RNAiso Plus (TaKaRa Bio Inc., Otsu, Japan) and cDNA was synthesized with the PrimeScript RT reagent Kit with the gDNA Eraser (TaKaRa Bio Inc.) ...
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bioRxiv - Molecular Biology 2020Quote: ... g12777 mRNA was selected by PCR using Tks Gflex DNA Polymerase (Takara) with the following primers:
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bioRxiv - Neuroscience 2020Quote: ... We reverse transcribed mRNA and amplified cDNA using Smart-seq V4 (Clontech), prepared sequencing libraries using Nextera XT (Illumina) ...
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bioRxiv - Developmental Biology 2022Quote: ... mRNA was captured and amplified with the Smart-seq2 v4 kit (Clontech). After AMPure XP beads purification ...
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bioRxiv - Molecular Biology 2024Quote: ... mRNA was reverse transcribed to cDNA with the PrimeScript RT kit (TaKaRa). cDNA was analysed by real-time PCR with the Light Cycler 480 SYBR Green I Master Mix (Roche) ...
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bioRxiv - Developmental Biology 2020Quote: ... and mRNA was captured and amplified with the Smart-seq2 v4 kit (Clontech). After AMPure XP beads purification ...
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bioRxiv - Developmental Biology 2022Quote: ... and mRNA was captured and amplified with the Smart-seq2 v4 kit (Clontech). After AMPure XP beads purification ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The mRNAs were reverse transcribed into cDNA by PrimeScript RT reagent Kit (Takara). The cDNA was amplified by a fast two-step amplification program using ChamQ Universal SYBR qPCR Master Mix (Vazyme Biotech Co. ...