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Citations for Takara Bio :
151 - 200 of 1209 citations for Human METTL3 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2021Quote: ... coli BL21 (DE3) Gold cells harboring the corresponding expression plasmid and the TaKaRa chaperone-plasmid pTF16 (TAKARA BIO INC.) were used to inoculate 500 mL of autoinduction medium (464 mL LB medium ...
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bioRxiv - Cell Biology 2020Quote: ... the sgRNA plasmid and a plasmid expressing the Cas9 nuclease were transfected using the Xfect Transfection Reagent (631318, Takara). A Cas9 expression plasmid without a puromycin resistance was generated by removing puromycin from pAc-sgRNA-Cas9 (addgene# 49330) ...
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bioRxiv - Cancer Biology 2022Quote: ... the recombinant pAAV-CRE plasmid was packaged in HEK293T cells using pHelper and pRC2-mi342 plasmids (Takara, Catalog #632608). Cells were harvested three days after transfection and AAV2 was isolated using AAVpro Extraction Solution (Takara ...
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bioRxiv - Neuroscience 2023Quote: ... mCherry was introduced into the pcDNA™3.1-BspEI-LRP10 plasmid via PCR amplification from the pmCherryN1 plasmid (Clontech) via the addition of BspEI restriction sites in the primer sequences ...
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bioRxiv - Genomics 2023Quote: ... The recombinant pAAV-CRE plasmid was packaged in HEK293T cells using pHelper and pRC2-mi342 plasmids (Takara, Catalog #632608). Cells were harvested three days after transfection and AAV2 was isolated using AAVpro Extraction Solution (Takara ...
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bioRxiv - Cell Biology 2020Quote: The cDNA coding sequences of the first and second cytoplasmic loop domain of human TMEM39A were cloned into the pGBKT7 vector and screened against a normalized universal human cDNA library (Clontech, 630481), following instruction of the Matchmaker® Gold Yeast Two-Hybrid System (Clontech ...
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bioRxiv - Genetics 2020Quote: The cDNA coding sequence of the C-terminal domain of human TMEM132D was cloned into the pGBKT7 vector and screened with a normalized universal human cDNA library (Clontech, 630481) in pGADT7 Vector ...
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bioRxiv - Cancer Biology 2023Quote: Lentiviral vectors for expression of LDHA or LDHB were generated by PCR amplification of the respective cDNAs from plasmids obtained from the Eugene McDermott Center for Human Growth and Development Sequencing Core Human ORF Collection and cloning into pLVX-IRES-Puro (Takara 632183) through Gibson assembly (NEB E2621) ...
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bioRxiv - Cell Biology 2023Quote: ... and of human FAM104B isoform 3 (NM_001166700.2) were PCR- amplified from a yeast two-hybrid human testis cDNA library (Clontech, cat. no. 638810) and cloned via appropriate restriction sites into pGAD-C1 (James et al ...
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bioRxiv - Evolutionary Biology 2021Quote: ... then removed from the pGEM shuttle plasmid using EcoRI and NotI enzymes and annealed into the DFRS empty plasmid pre-amplified (DFRS empty plasmid primers) and digested by the same enzymes (In-Fusion Kit; Clontech). The control cassette was thus placed on the 3’UTR of the RFP gene.
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bioRxiv - Microbiology 2021Quote: ... The retroviral reporter plasmids encoding a Gaussia luciferase reporter gene were constructed by cloning the reporter genes into the pQCXIP plasmid (Clontech). DNA fragments encoding C-terminally S-tagged ACE2 orthologs were synthesized in pUC57 backbone plasmid by Sangon Biotech (Shanghai ...
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bioRxiv - Cell Biology 2021Quote: ... GCN4-scFv-sfGFP were amplified from addgene plasmid #60906 and introduced into plasmid#132397 through in-fusion cloning (Takara Bio). All other plasmids in this study are derived from a plasmid that contains a CAG promoter for constitutive expression ...
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bioRxiv - Microbiology 2022Quote: ... Each of these prey plasmids and previously constructed bait plasmids were co-transformed into Y2HGold yeast strain using Yeastmaker Yeast Transformation System 2 (TaKaRa). The transformation mix was spread on DDO/X and QDO/X/A agar plates and incubated at 30°C for 3-5 days ...
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bioRxiv - Cell Biology 2020Quote: ... was co-transfected with packaging plasmid (pCAG-HIVgp) and VSV-G/Rev-expressing plasmid (pCMV-VSVG-RSV-Rev) into HEK293T cells (Clontech). Cells expressing Involucrin-mCherry were selected by 2 µg/ml Puromycin treatment.
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bioRxiv - Genetics 2019Quote: ... To generate the plasmid pEGFP-N2-pB-min we removed the multiple cloning site (MCS) of the pEGFP-N2 plasmid (Clontech) by BamHI-NheI double digestion ...
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bioRxiv - Microbiology 2023Quote: ... The retroviral reporter plasmids encoding a Gaussia luciferase reporter gene were constructed by cloning the reporter genes into the pQCXIP plasmid (Clontech). Plasmids encoding soluble ACE2 variants fused with human IgG1 Fc were described in our previous study20 ...
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bioRxiv - Cancer Biology 2023Quote: ... The generated lentiviral plasmids were cotransfected with viral packaging plasmids PAX2 and pMD2.G into HEK 293T Lenti-X cells (Clontech) using TransIT-LT1 (Mirus Bio LLC ...
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bioRxiv - Cancer Biology 2024Quote: ... approximately 20% confluent HEK293T cells seeded in 12-wells plates were transfected with 300ng ZNRF3 encoding plasmid and 50ng GFP expression plasmid (pEGFP-C1, Clontech) using Lipofectamine 2000 ...
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bioRxiv - Physiology 2024Quote: The pGG2-TNNT2-HA-TTL and the pAAV-TNNT2-Empty plasmids were generated from the pGG2-TNNT2-Mut1 plasmid (VC206) by InFusion Cloning (Takara Clontech). In brief ...
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bioRxiv - Plant Biology 2019Quote: ... were transferred into the pCold ProS2 plasmid (TaKaRa) harboring a gateway reading flame cassette (Invitrogen) ...
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bioRxiv - Molecular Biology 2020Quote: ... pECFP and pEYFP plasmids were purchased from Clontech Laboratories (Palo Alto ...
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bioRxiv - Biochemistry 2019Quote: ... This plasmid was used along with pGro7 (Takara) to transform Shuffle T7 Express cells (NEB) ...
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bioRxiv - Bioengineering 2021Quote: ... The original pAUR101 plasmid was purchased from Takara Bio ...
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bioRxiv - Biochemistry 2020Quote: pEGFP-N1 plasmid (Clontech, Mountain View, California, US) was transformed into XL1-Blue [dut+ ...
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bioRxiv - Biochemistry 2021Quote: ... plasmid backbones derived from EYFP-N1 (Clontech/Takara), Lenti-Cas9-blast (a kind gift of F ...
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bioRxiv - Biochemistry 2021Quote: ... plasmid backbones derived from EYFP-N1 (Clontech/Takara), Lenti-Cas9-blast (a kind gift of F ...
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bioRxiv - Neuroscience 2022Quote: ... were transformed with the plasmid peGFP-C1 (Clontech). Bacteria were grown overnight in lysogeny broth (LB ...
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bioRxiv - Cell Biology 2022Quote: Plasmid DNA (pEGFP-N1, 5kb (Clontech, 6085-1) encoding a cytomegalovirus (CMV ...
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bioRxiv - Cell Biology 2020Quote: ... packaging plasmid using CalPhos Mammalian Transfection Kit (Takara). Next day ...
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bioRxiv - Bioengineering 2021Quote: ... The original pAUR101 plasmid was purchased from Takara Bio ...
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bioRxiv - Molecular Biology 2019Quote: ... Two-hybrid plasmids were based on pGBT9 (Clontech) or the pGAD-C- and pGBD-C-series ...
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bioRxiv - Developmental Biology 2019Quote: ... The plasmids were then digested with DNaseI (Takara) for 30 min at 37°C ...
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bioRxiv - Developmental Biology 2020Quote: ... from pEGFP-C1 and pDsRed2-N1 plasmid (Clontech). The fragment bounded by the AseI and NheI restriction sites was deleted from pEGFP-C1 and pDsRed2-N1 and the upstream sequences of Atoh7 (2220 bp in length ...
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bioRxiv - Biochemistry 2020Quote: ... Five micrograms of reporter plasmid pEGFP-C2 (Clontech), coding the green fluorescent protein ...
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bioRxiv - Neuroscience 2019Quote: ... Additional plasmids were created using InFusion cloning (Takara). The InFusion cloning module (SnapGene ...
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bioRxiv - Neuroscience 2020Quote: ... the CMV-EGFP plasmid (pEGFP; Clontech, CA, USA) was used as a positive control ...
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bioRxiv - Immunology 2021Quote: ... A plasmid encoding tdTomato was obtained from Takara Bio (#632533 ...
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bioRxiv - Cell Biology 2021Quote: Plasmids used for transfections include pmCherry-C1 (Clontech), pLAMP1-mGFP (Addgene #34831 ...
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bioRxiv - Cell Biology 2023Quote: ... plasmid backbones derived from EYFP-N1 (Clontech/Takara), Lenti-Cas9-blast (a kind gift of F ...
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bioRxiv - Cell Biology 2023Quote: ... plasmid backbones derived from EYFP-N1 (Clontech/Takara), Lenti-Cas9-blast (a kind gift of F ...
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bioRxiv - Biochemistry 2023Quote: The reporter plasmids were linearized with XbaI (Takara). Subsequently ...
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bioRxiv - Neuroscience 2023Quote: ... mCherry-C1 plasmid (Clontech, Mountain View, CA, USA) at 1 ng/μl ...
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bioRxiv - Immunology 2023Quote: ... plasmids were transformed into Stellar cells (Takara Bio), isolated by Maxiprep kits (Macherey Nagel) ...
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bioRxiv - Physiology 2024Quote: The plasmids were linearized with NotI (Takara Bio), and capped RNAs (cRNAs ...
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bioRxiv - Cell Biology 2023Quote: ... pEGFP-C1 plasmid construct was purchased from Clontech. Flag-CSA construct subcloned in pcDNA3.1 vector was a kind gift Dr ...
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bioRxiv - Cancer Biology 2020Quote: ... Human total RNA from different normal tissues from Clontech (Human Total RNA Master Panel II,Cat# ...
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bioRxiv - Cell Biology 2019Quote: ... and human BaxS184V was cloned in pEGFP-C1 (Clontech). Bax/Bak DKO MEFs were grown in DMEM supplemented with 10% FBS ...
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bioRxiv - Biochemistry 2019Quote: ... Human colon cDNA purchased from Clontech (Palo Alto, CA) was used as the template ...
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bioRxiv - Neuroscience 2020Quote: ... anti-human nuclei (STEM101; Takara, Y40400, IgG1, 1:100) and anti-human NOGOA (Santa Cruz Biotechnology ...
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bioRxiv - Developmental Biology 2021Quote: ... and human-specific GFAP marker STEM123 (TaKaRa, cat. Y40420). As general astrocyte markers GFAP (DAKO ...