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Citations for Takara Bio :
401 - 450 of 1209 citations for Human METTL3 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: The human kidney epithelial cell line Lenti-X 293T was purchased from Takara. The human liver cell line Huh7.5.1 was provided by Dr ...
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bioRxiv - Immunology 2020Quote: The human epithelium kidney 293T Lenti-X cells (Clontech Laboratories, Mountain View, CA) were maintained in Dulbecco’s Modified Eagles medium (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... was inducibly expressed in Lenti-x-293T human female kidney cells from Takara Bio (Catalog # ...
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bioRxiv - Cell Biology 2020Quote: ... S5C-E was generated by fusing human Rac1 cDNA into EGFP-C1 (Clontech) and kindly provided by Dr ...
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Enterohepatic Transcription Factor CREB3L3 Protects Atherosclerosis via SREBP Competitive InhibitionbioRxiv - Physiology 2020Quote: ... mCherry-tagged human SREBP-1c was inserted into pmCherry (mCherry-SREBP-1c) (Clontech), and HA-tagged hamster SCAP ...
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bioRxiv - Molecular Biology 2021Quote: ... was inducibly expressed in Lenti-x-293T human female kidney cells from Takara Bio (Catalog # ...
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bioRxiv - Cancer Biology 2019Quote: Human NIC4 was sub-cloned into pEGFP-N3 (BD Clontech, Mountain View, CA) between EcoRI and BamHI restriction sites to obtain NIC4-GFP using the following primers:
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bioRxiv - Cell Biology 2019Quote: ... The sequences encoding human Rap1a(Q63E) and Rap1GAP1 were cloned into p3xFlag7.1(-) (Clontech). Transient transfection was performed using TransIT-LT1 Reagent (Mirus ...
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bioRxiv - Neuroscience 2020Quote: ... the human cytomegalovirus (hCMV) promoter/immediate-early enhancer (IE) of peGFP-N1 (Clontech) and the chimeric intron (chI ...
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bioRxiv - Cell Biology 2021Quote: ... Primer sequences (Table 2) were verified using total human kidney RNA (Takara Bio). PSMB4 was determined as the most stable housekeeping gene using the method described by Xie ...
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bioRxiv - Immunology 2021Quote: 293T Lenti-X human embryonic kidney cell line (Clontech, Mountain View, CA, USA) was used for LV or IDLV production by transient transfection as previously described22,58 ...
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bioRxiv - Genomics 2022Quote: ... Total RNA pools from human brain (cat. no. 636530, Takara Bio USA, Inc), small intestine (cat ...
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bioRxiv - Biochemistry 2023Quote: ... Human embryonic kidney (HEK) 293T Lenti-X were obtained from Takara (cat# 632180). These cells transformed with adenovirus type 5 DNA and expresses the SV40 large T antigen ...
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bioRxiv - Neuroscience 2023Quote: Human neurons were transfected on DIV28 with pEGFP-C1 (0.3 μg/well, Clontech) plus vectors expressing mCherry ...
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bioRxiv - Biochemistry 2023Quote: ... Lenti-X™ 293T cell line (human, female origin) were purchased from TaKaRa and used without further authentication ...
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bioRxiv - Molecular Biology 2023Quote: ... Cloning of the human SIK2 coding sequence to the pEGFP-C3 (Clontech, USA) vector was previously described by Küser-Abali et al. ...
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bioRxiv - Neuroscience 2024Quote: The primary human fibroblasts (HFs) and the corresponding iPSCs were purchased from Takara Bio (Kusatsu ...
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bioRxiv - Microbiology 2020Quote: ... The ligated plasmid was then transformed to Escherichia coli HST08 (Takara Bio) and selected on chloramphenicol plates ...
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bioRxiv - Microbiology 2021Quote: ... and then was cloned in the plasmid pMD19-T simple (TAKARA, Japan). The DNA fragment was digested with BamHI/XhoI (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... pMD2.G and the CAR-expression plasmid by calcium phosphate transfection (Clontech). At 16 hrs post-transfection cells were washed with PBS and incubated in complete DMEM media containing 6 mM sodium butyrate (Sigma Aldrich) ...
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bioRxiv - Synthetic Biology 2022Quote: ... A middle-copy (∼10-30 copies) episomal plasmid pGADT7 (Takara Bio Inc.) was used to increase the concentration of proteins to buffer for the effects of intrinsic molecular noise and selected using different auxotrophic selection markers (Leucine ...
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bioRxiv - Cell Biology 2022Quote: All plasmids used were generated using the In-Fusion cloning system (Takara) with primers containing 15 bp 5’ flanking regions complementary to the free ends of the cloning vector ...
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bioRxiv - Genomics 2020Quote: ... After both ends of the plasmids were blunted using Klenow fragments (Takara), they were ligated by DNA ligase (Takara).
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bioRxiv - Cell Biology 2019Quote: Sources of plasmids encoding the following proteins were: mCherry-C1 (Clontech #632524); mCherry-N1 (Clontech #632523) ...
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bioRxiv - Immunology 2021Quote: ... and NF-kB reporter plasmids using X-fect Transfection reagent (Takara, Japan) following manufacturer’s protocol ...
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bioRxiv - Neuroscience 2020Quote: ... The transgene plasmid was generated using the InFusion HD Kit (Clontech, 638910) and the NucleoBond Xtra Midi EF Kit (Macherey-Nagel ...
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bioRxiv - Biochemistry 2020Quote: ... The resultant plasmids were transformed into Stellar chemically competent cells (Clontech Laboratories), selected with carbenicillin ...
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bioRxiv - Neuroscience 2021Quote: Cloned plasmids were packaged in Lenti-X 293T cells (Takara Biosciences #632180), transfected using Lipofectamine 2000 (Thermo #11668027) ...
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bioRxiv - Plant Biology 2021Quote: ... The resulting plasmid was then used for In-Fusion reaction (Takara Clontech) for inserting PCR-amplified GFP fragment at the C-terminus of AtSMOS1 ...
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bioRxiv - Plant Biology 2021Quote: ... The resulting plasmid was then used for In-Fusion reaction (Takara Clontech) to insert PCR-amplified GFP fragments at the C-terminus of the SCL28 coding sequence ...
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bioRxiv - Plant Biology 2021Quote: ... The resulting plasmid was then used for In-Fusion reaction (Takara Clontech) for inserting PCR-amplified GFP fragment at the C-terminus of AtSMOS1 ...
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bioRxiv - Plant Biology 2021Quote: ... The resulting plasmid was then used for In-Fusion reaction (Takara Clontech) to insert PCR-amplified GFP fragments at the C-terminus of the SCL28 coding sequence ...
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bioRxiv - Neuroscience 2019Quote: ... and subcloned into the Cry2.mCh mammalian expression plasmids (mCherry-N1 (Clontech)) via restriction digest and ligation ...
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bioRxiv - Microbiology 2021Quote: ... were co-transformed with the plasmids pET-28b_atsJ and pG-KJE8 (TAKARA). A 200 ml culture in TB medium (50 μg/ml kanamycin ...
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bioRxiv - Microbiology 2020Quote: ... Transformation with plasmids and cultivation were performed using standard techniques (Clontech manual). In addition to negative and positive controls included in the MatchMaker III system ...
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bioRxiv - Cell Biology 2020Quote: The following plasmids were used in this study: pCDNA (control vector) (Clontech), pCDNA3-EGFP (Addgene Plasmid #13031) ...
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bioRxiv - Microbiology 2022Quote: ... and the PBSU101 plasmid was digested by BamHI and XbaI (Japanese Takara). Then PBSU101-pykF ...
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bioRxiv - Cancer Biology 2022Quote: The retroviral plasmid pRetroX-TetOne puro (634307, Clontech, Mountain View, CA, USA) was amplified using NEB Stable competent Escherichia coli (high efficiency ...
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bioRxiv - Immunology 2022Quote: ... Ligated plasmids were then transformed into Stellar competent cells (Takara Clontech, 636763) and single clones were picked on the next day ...
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bioRxiv - Immunology 2022Quote: ... Ligated plasmids were then transformed into Stellar competent cells (Takara Clontech, 636763) and single clones were picked on the next day ...
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bioRxiv - Neuroscience 2019Quote: ... The cloned plasmids were transformed into Stellar competent cells (Takara Bio, Japan), and 200 clones per rabies virus sample were isolated and purified for sequencing ...
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bioRxiv - Neuroscience 2019Quote: ... Plasmids used in this study were: pEGFP-N1 (Clontech, Mountain View, California); Flag-CNTNAP2 (generated by subcloning human CNTNAP2 cDNA from a plasmid kindly provided by Dr ...
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bioRxiv - Cell Biology 2019Quote: pEGFP-N1 (EGFP) and mCherry-C1 (mCherry) plasmids were purchased from Clontech Laboratories ...
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bioRxiv - Biochemistry 2019Quote: Recombinant DmNobo was expressed with the pCold-III plasmid vector (TaKaRa Bio) in Escherichia coli strain BL21(DE3 ...
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bioRxiv - Cell Biology 2020Quote: ... The sequence was ligated into the pLVX Puro lentiviral plasmid (Clontech, CA) using the same restriction sites and sequenced to confirm correct orientation ...
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bioRxiv - Cell Biology 2021Quote: ... Plasmids were constructed using In-Fusion HD EcoDry Cloning Kits (Takara 121416), NEB HiFi assembly (New England Biolabs E5520S) ...
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bioRxiv - Immunology 2020Quote: The pLVX-IRES-ZsGreen1 plasmid (Takara Bio USA, Inc., Mountain View, CA) was used to generate construct encoding BiTE cDNA ...
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bioRxiv - Microbiology 2021Quote: ... and then was cloned in the plasmid pMD19-T simple (TAKARA, Japan). The DNA fragment was digested with EcoRI/XhoI and BamHI/EcoRI (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... The assembled plasmids were amplified by transformation of Stellar Competent Cells (Takara) and purified from overnight cultures in LB medium containing 100 μg/ml Ampicillin grown at 37°C by using the NucleoSpin Plasmid isolation kit (Macherey-Nagel) ...
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bioRxiv - Systems Biology 2022Quote: ... with pMI-NLS-Cre-ires-Tomato plasmid using the CalPhos kit (Clontech). Retroviral supernatants were harvested after 48hrs ...