Labshake search
Citations for Takara Bio :
4801 - 4850 of 4900 citations for mu p75 SAP Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: RT-PCR was performed in a single closed tube using a one-step RT-PCR kit (One Step PrimeScript III RT-qPCR Mix, with UNG; Takara Bio Inc., Kusatsu, Japan) in accordance with the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... qPCR expression analysis was conducted on cDNA derived from immunoprecipitated RNA of OT:RiboTag mice following reverse (SMARTer® PCR cDNA synthesis kit; Takara Bio Inc., Shiga, Japan).
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bioRxiv - Plant Biology 2022Quote: ... Real-time PCR was performed on an ABI Prism 7500 Fast Real-Time PCR System using the SYBR Premix Ex Taq kit (Takara Bio Inc., Shiga, Japan) according to the instructions ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The ATP1A1 genes were inserted at the PPH promoter of vectors already containing the corresponding ATP1B1 genes using In-Fusion® HD Cloning Kit (Takara Bio; Cat#638910) and confirmed by sequencing ...
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bioRxiv - Neuroscience 2022Quote: ... Total RNA was reverse transcribed in the presence of PrimeScript™ RT reagent Kit with gDNA Eraser (Perfect Real Time) (Takara, Cat. No. RR047B). For each RT-qPCR reaction ...
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bioRxiv - Neuroscience 2022Quote: ... Total mRNA was reverse transcribed in the presence of PrimeScript™ RT reagent Kit with gDNA Eraser (Perfect Real Time) (Takara, Cat. No. RR047B). Droplet digital PCR was used to measure the levels of transcription factors in isolated MG following a previous method (Fisher et al. ...
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bioRxiv - Microbiology 2022Quote: ... The resulting PCR product was then cloned into pVV16-hsp60 between NdeI and HinDIII using In-Fusion® HD cloning kit (Takara Bio USA, Inc) and Stellar™ competent cells ...
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bioRxiv - Molecular Biology 2022Quote: ... Section B step 4 in the protocol was modified to account for the incorporation of UDIs (Takara SMARTer RNA Unique Dual Index Kit-96A). A total of 2 uL of each UDI was used instead of the recommended 1 uL each of the 5’ and 3’ PCR primers ...
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bioRxiv - Cell Biology 2024Quote: ... Ten nanograms of RNA were used for preparing indexed libraries using SMARTer Stranded Total RNA-Seq Pico-Input Mammalian kit v.3 (Takara Bio. Cat# SKU: 634487) as per manufacturer’s instructions (except that fragmentation was performed for 3 minutes of fragmentation at 94 °C and 13 cycles was used for PCR2) ...
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bioRxiv - Neuroscience 2024Quote: ... The Genomic and RNA Profiling Core prepared libraries with the Takara SMARTer® Stranded Total RNA-Seq v3 - Pico Input Mammalian kit (Takara, p/n 634485). Using the KAPA Library Quantification kit for Illumina platforms (KAPA Biosystems ...
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bioRxiv - Immunology 2024Quote: ... reverse transcription and PCR steps were directly performed after sorting according to the SMART-Seq® v4 Ultra® Low Input RNA Kit protocol (Takara Bio, USA). Amplified complementary DNA were transferred to the Benaroya Research Institute (Seattle ...
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bioRxiv - Neuroscience 2023Quote: Berghia brains (CRG plus rhg) were dissected and put into lysis buffer from the Smart-Seq4 kit (Takara Bio USA Inc., San Jose, CA) and stored at -20°C ...
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bioRxiv - Immunology 2023Quote: Ultra-low input RNA sequencing libraries (from sorted Tgfb1fl/fl;Cx3cr1Cremicroglia) were prepared by using SMART-Seq HT kit for full-length cDNA synthesis and amplification (Takara, San Jose, CA, USA), and Illumina Nextera XT (Illumina ...
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bioRxiv - Molecular Biology 2024Quote: ... From cortical neurons subjected to OGD we generated 1) library to detect circularRNAs and mRNA and lncRNAs using SMARTer® Stranded Total RNA Sample Prep Kit (Takara Bio USA, Inc.) and 2 ...
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bioRxiv - Biochemistry 2023Quote: ... The PCR fragments of spike protein DNA were cloned into linearized pMRNAXP vector using In-Fusion® HD Cloning Kit (Clontech® Laboratories, Inc.). The cloning mixtures were transformed to One Shot™ TOP10 Chemically Competent E ...
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bioRxiv - Bioengineering 2024Quote: ... RNA concentrations were normalized and reverse transcription was performed using the RNA to cDNA EcoDry premix kit (Takara Bio USA; San Jose, CA, USA). Taqman universal master mix and Taqman probes (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... A total of 1 µg of RNA was reverse transcribed to complementary DNA using the PrimeScript™ RT reagent Kit (Takara Bio, Otsu, Japan), according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2023Quote: The cDNA prepared from 10-day-old OsbZIP48OE seedlings was used to perform 5’ and 3’ RACE using SMARTer® RACE 5’/3’ Kit (Clontech, TaKaRa Bio, USA) as per manufacturer’s instructions followed by Sanger based sequencing of the RACE products ...
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IRF1 regulates self-renewal and stress-responsiveness to support hematopoietic stem cell maintenancebioRxiv - Cell Biology 2023Quote: ... cDNA generation and amplification were performed using the SMART-Seq® v4 Ultra® Low Input RNA Kit for sequencing (Takara Bio USA, Inc.). Quality check was performed using High sensitivity D5000 ScreenTape® on TapeStation Analysis Software 3.2 (Agilent Technologies ...
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bioRxiv - Molecular Biology 2022Quote: ... 0.5 µg of quality checked total RNA was converted into first-strand cDNA using a PrimeScript RT Reagent Kit with gDNA Eraser (TaKaRa Bio Inc, Shiga, Japan), according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... 5′-GCATGTCACGATGTTACAA-3′ and 5′-GGATGAAATGAAGGTGCTA-3′ as previously described50 and were inserted using the Infusion HD Cloning kit (Takara Bio USA Inc, NC1470242).
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bioRxiv - Biochemistry 2023Quote: ... and the resulting DNA sequences were synthesized (Twist Bioscience, California, USA) and cloned into the pET-28a(+) plasmid using In-Fusion cloning kit (Takara Bio Inc., Shiga, Japan) or the iVEC3 method (58) ...
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bioRxiv - Bioengineering 2023Quote: ... The synthesized gene fragments were then cloned into the linear pET21a vector using the In-Fusion® HD Cloning Kit (Takara Bio, Kusatsu, Japan). For mammalian expression ...
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bioRxiv - Developmental Biology 2023Quote: ... RNA isolation and cDNA synthesis were carried out using the SMARTer® Ultra® Low Input RNA Kit for Sequencing – v4 (Clontech, Cat.no. 634888). Libraries were generated from the resulting cDNA (0.2ng/ul per sample ...
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bioRxiv - Plant Biology 2023Quote: ... One microgram of total RNA was used to synthesize cDNA using Oligo dT primers and Smart MMLV Reverse Transcriptase kit (Takara Bio Inc, Shiga, Japan). The expression of NIa-Pro was verified in all samples using gene specific primers and RT-PCR (Fig S1a ...
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bioRxiv - Microbiology 2023Quote: ... The PCR reactions at every examined depth were performed in triplicate (n=3) using Takara SpeedSTAR HS DNA polymerase kit (Takara Bio USA, Madison, WI) with the following modifications ...
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bioRxiv - Microbiology 2023Quote: ... RNA was extracted from the injected aphid nymphs 24 h post-injection using the Takara Nucleospin RNA Plus XS RNA extraction kit (Takara Bio Inc., Shiga, JP). For RNA extraction ...
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bioRxiv - Cell Biology 2023Quote: ... The reporter cassette excised from pPExRF3 and the left and right homology arms were assembled and cloned into a linearized pUC19 in a single enzymatic reaction using the In-Fusion Cloning Kit (Clontech Laboratories Inc., California, U.S.A). A gRNA vector was constructed by cloning a 20-bp target sequence surrounding the stop codon of coronin into pU6 (FlyCRISPR) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... RNA input was normalized across samples and RNA libraries were prepared utilized the SMART-Seq® v4 Ultra® Low Input RNA Kit for Sequencing (Takara, 634890, 634888) followed by the Nextera XT DNA library preparation kit (Illumina ...
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bioRxiv - Cell Biology 2024Quote: ... The PCR product was cloned into the KpnI site of the pCRtk2×2NN vector (RDB18670) to generate pCRtk-PDX1 using an In-Fusion HD Cloning Kit (639648; Takara Bio Inc., Shiga, Japan). Subsequently ...
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bioRxiv - Cell Biology 2024Quote: ... cDNA from the same amount of RNA between RNAi and control samples were synthesized in a 20 μl reaction volume by the PrimeScript™ RT reagent Kit with gDNA Eraser (TAKARA, Code No. RR047A). One μl of cDNA was used as the template in a 10 μl reaction volume of the PowerUp SYBR Green Master Mix (Applied Biosystems ...
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bioRxiv - Neuroscience 2024Quote: ... Ultra-low input RNA sequencing library was prepared by using SMART-Seq HT kit for full-length cDNA synthesis and amplification (Takara, San Jose, CA, USA), and Illumina Nextera XT (Illumina ...
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bioRxiv - Microbiology 2024Quote: ... The amplified linear fragment of pKNOCK-Km and the internal fragments of each gene were fused using In-Fusion® HD Cloning Kit (Takara Bio, Shiga, Japan) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The rRNA genes (18S, ITS, 28S) were amplified by polymerase chain reaction (PCR) using the TaKaRa Ex Taq polymerase kit (TaKaRa Bio-medicals, Seoul, Korea). PCR cycling parameters and primer information were followed as in Shazib et al ...
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bioRxiv - Evolutionary Biology 2024Quote: ... WTAs of the individual ciliates were generated according to the Smart-seq2 protocol (Picelli et al. 2014) and SMART-Seq v4 Ultra Low Input RNA Kit (TaKaRa Bio-medicals, Seoul, Korea) using 17 cycles of cDNA amplification for each cell ...
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bioRxiv - Immunology 2022Quote: ... 0.5 µg of the isolated RNA was reverse transcribed to cDNA using an oligo dT primer and 12.5 units of reverse transcriptase mix (PrimeScript RT Reagent Kit, TaKaRa Bio, Saint-Germain-en-Laye, France). cDNA corresponding to 10 ng total RNA served as the template in a real-time PCR ...
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bioRxiv - Molecular Biology 2020Quote: ... was amplified by RT-PCR and cloned into pMSCV-IRES-eGFP using In-Fusion® HD Cloning Kit User Manual (Takara, Cat#638920, Shiga, Japan) following the manufacturer’s instructions (see Table 3 for primer sequences).
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bioRxiv - Immunology 2019Quote: ... Library construction was performed with the “SMARTer® Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian” (TaKaRa Bio USA, Inc., Mountain View, CA, USA) with a final multiplexing of 11 libraries according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2019Quote: ... to perform 5′ and 3′ rapid amplification of cDNA ends (RACE)-PCR utilizing the Clontech SMARTer 5′/3′ RACE Kit (Takara BIO USA Inc, CA, USA) as recently described58 ...
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bioRxiv - Microbiology 2020Quote: ... was reverse-transcribed to cDNA using random hexamers and oligo (dT) by following the instructions provided in the Prime-Script RT Reagent Kit (Perfect Real Time from TaKaRa Bio Inc., Otsu Shiga, Japan). RT-qPCR was carried out in a StepOnePlus Real-Time PCR system (Applied Biosystems ...
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bioRxiv - Systems Biology 2020Quote: ... RNA was eluted in 10 µl water and immediately proceeded to cDNA generation using the SMART-Seq V4 Ultra Low RNA Kit (Takara Bio USA, Mountain View, CA). 1 ng of the resulting cDNA from 14 PCR cycles was “tagmented” and barcoded by using a Nextera XT DNA Sample Preparation Kit (Illumina) ...
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bioRxiv - Microbiology 2021Quote: ... recombinant sites including the 900 base pair upstream/downstream flanking region and the 100 base pair coding sequence of the target genes were cloned into pK18-CmR-pheS** plasmid backbone by using an In-Fusion® HD Cloning Kit (TAKARA Bio; Kusatsu, Shiga, Japan) to generate the plasmid (aedF- ...
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bioRxiv - Zoology 2021Quote: ... The reverse transcription of 1 μg of total RNA was performed using PrimeScript® RT reagent kit with gDNA Eraser (RR047A, Takara Bio Inc., Dalian, China). The gene expression was determined through RT-PCR ...
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bioRxiv - Biochemistry 2022Quote: ... one microgram of total RNA was employed to obtain the corresponding cDNA target sequences using an oligo(dT)18 primer and the PrimeScript RT reagent kit (Perfect Real Time, Takara Bio Inc., Otsu, Shiga, Japan), following the manufacturer’s directions ...
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bioRxiv - Microbiology 2021Quote: Antibody sequencing was performed as previously described (Simonich et al., 2019; Vigdorovich et al., 2016) using the SMARTer RACE 5’/3’ Kit (Takara Bio USA Inc., Mountainview, CA). Briefly ...
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bioRxiv - Evolutionary Biology 2022Quote: ... library was prepared according to the Isoform Sequencing protocol (Iso-Seq) using the Clontech SMARTer® PCR cDNA Synthesis Kit (Clontech Laboratories (now Takara Laboratories), 634926 ...
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bioRxiv - Microbiology 2019Quote: ... 300 ng of each RNA was reverse transcribed to cDNA using random hexamers and oligo(dT) primers and following the instructions provided in the PrimeScript RT Reagent Kit (Perfect Real Time from Takara Bio Inc., Otsu Shiga, Japan). RT-qPCR was carried out in a StepOnePlus Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Neuroscience 2021Quote: ... Flash frozen cell samples were sent to the Cambridge Genomic Services for library preparation using SMART-Seq® v4 Ultra® Low Input RNA Kit (Takara Bio USA, Inc), followed by RNA sequencing (NextSeq 74 cycle high output 15-20 M reads per sample ...
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bioRxiv - Physiology 2020Quote: ... The PCR products were subsequently cloned into an EcoRI-HF® (New England BioLab, MA, USA) linearized pIRESZ2_ZsGreen1 vector using In-Fusion® HD cloning kit (TaKaRa Bio Inc, Kusatsu, JP). After sequence validation (Eurofin ...
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bioRxiv - Genetics 2020Quote: ... nucleotides 610–792 of the map3 ORF were subjected to random mutagenesis by using the Diversify PCR Random Mutagenesis Kit (Clontech Laboratories, Mountain View, CA, USA) in accordance with the manufacturer’s instructions for error-prone PCR ...