Labshake search
Citations for Takara Bio :
4751 - 4800 of 5473 citations for Arg8 Vasopressin AVP ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... Total RNA was reverse transcribed using PrimeScriptTM RT reagent Kit with gDNA Eraser (Perfect Real Time) according to the manufacturer’s protocol (Takara, Kusatsu, Japan). DNA and cDNA samples were subjected to 16S rRNA gene amplicon sequencing ...
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bioRxiv - Plant Biology 2021Quote: ... was used for the first strand cDNA synthesis following the manufacturer’s instructions (SMARTer RACE cDNA Amplification Kit (Clontech, Cat. No.634923)) ...
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bioRxiv - Plant Biology 2021Quote: ... First-strand cDNA was synthesised from 1 μg of total RNA using PrimeScript 1st-Strand cDNA Synthesis Kit (Takara Bio, Japan) as per manufacturer’s protocol ...
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bioRxiv - Physiology 2020Quote: ... 1μg RNA was reverse transcribed into single-stranded cDNA using the SuperScript First-strand Synthesis Kit (Takara Bio Inc., Tokyo, Japan). Gene-specific primers were designed by Beacon Designer 7.9 software (Premier Biosoft International ...
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Altered TGFB1 regulated pathways promote accelerated tendon healing in the superhealer MRL/MpJ mousebioRxiv - Cell Biology 2021Quote: Samples of 1ng of total RNA were pre-amplified with the SMARTer Ultra Low Input kit v4 (Clontech, Mountain View, CA) per manufacturer’s recommendations ...
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bioRxiv - Microbiology 2020Quote: ... The cloned fragment was inserted into the plasmid pPICZαA containing the AOX1 promoter by replacing the fragment between the EcoR I site and Xba I site using an In-Fusion HD cloning kit (Clontech, Japan). To express recombinant proteins with a fluorescent tag ...
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bioRxiv - Microbiology 2020Quote: ... The PCR products were purified and fused into a ClaI-digested plasmid with an In-Fusion HD cloning kit (TAKARA, Japan). Mutants for overexpressing HFB2 (TgOPB38530 ...
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bioRxiv - Neuroscience 2021Quote: ... 1 μg total RNA was used for cDNA synthesis using RNA to cDNA EcoDry™ Premix (double-primed) kits (Clontech, UK). qRT-PCR reactions were performed in triplicate using a Corbett Rotorgene 6000 Real-Time PCR machine with Sensimix SYBR No-Rox (Bioline ...
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bioRxiv - Genomics 2021Quote: ... The IVT RNA is transferred into cDNA with random primers with SMART MMLV kit by following the manufactory protocol (TaKaRa, 639524). 100ng RNA was used for each library preparation ...
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bioRxiv - Microbiology 2020Quote: ... was amplified using primers C.9 and C.10 and cloned into pOPINF using the In-Fusion HD EcoDry cloning kit (Takara Bio).
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bioRxiv - Physiology 2021Quote: ... First-strand cDNA was synthesized from total RNA using a PrimeScript RT Reagent Kit with gDNA Eraser (Takara Bio, Shiga, Japan).
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bioRxiv - Biochemistry 2021Quote: ... Hsf1 CS mutant (C35S/C103S/C153S/C373S/C378S) was then constructed through site-directed mutagenesis using PrimeSTAR Mutagenesis Basal Kit (Takara Bio). All the protein samples were expressed in BL21(DE3 ...
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bioRxiv - Cell Biology 2021Quote: ... where mApple cDNA was inserted between Nhel/EcoRI sites and the GLI1 gene or its variants inserted into EcoRI downstream of mApple using the InFusion cloning kit (Takara Bio).
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bioRxiv - Microbiology 2021Quote: ... The copy number of viral RNA was standardized with a SARS-CoV-2 direct detection RT-qPCR kit (Takara, Cat# RC300A). The fluorescent signal was acquired using a QuantStudio 3 Real-Time PCR system (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... was used as the template for real-time RT-PCR performed according to the manufacturer’s protocol using the One Step TB Green PrimeScript PLUS RT-PCR kit (Takara, Cat# RR096A) and the following primers ...
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Stem cell-derived macrophages as a new platform for studying host-pathogen interactions in livestockbioRxiv - Immunology 2021Quote: ... Filtered lentivirus was either stored in aliquots at −80°C or further purified and concentrated using the Lenti-X Maxi Purification kit (Takara #631234) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... reverse transcription and cDNA amplification were performed on the Fluidigm C1 machine according to the manufacturer’s mRNA-seq protocol using the SMARTer Ultra Low RNA Kit for the Fluidigm C1 System (Takara Bio #634833). cDNA amplicons (∼3 µL ...
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bioRxiv - Developmental Biology 2021Quote: ... 350 ng RNA samples in 6 µL nuclease-free water) were generated using the Clontech SMARTer smRNA-Seq kit using 8 PCR cycles (Takara Bio). 30 µL of the PCR reaction was purified with AMPure XP beads (Beckman Coulter A63880 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... qRT-PCR was performed by using One Step SYBR® PrimeScript® PLUS RT-RNA PCR Kit (TaKaRa Biotechnology, Dalian, China). Relative expression levels were analyzed by using the 2−ΔΔCT method.
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bioRxiv - Biochemistry 2021Quote: ... falciparum cDNA followed by Ligation Independent Cloning into HindIII/KpnI-cleaved plasmid pOPIN F (82) using the In-Fusion HD EcoDry Cloning Kit (Takara Clontech) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... Pooled samples were processed using SMARTer chemistry (SMART Seq® v4 Ultra® Low Input RNA Kit for Sequencing, Takara Clontech), according to manufacturer’s instructions to generate cDNA libraries for mRNA sequencing ...
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ER exit sites in Drosophila display abundant ER-Golgi vesicles and pearled tubes but no megacarriersbioRxiv - Cell Biology 2021Quote: ... the coding sequence of each gene was amplified from whole larva cDNA using PrimeScript RT-PCR Kit (Takara, cat # RR014-A). The resulting products were recombined into pDONR221 and transferred to the pTWGA vector to finally produced the desired plasmids ...
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bioRxiv - Genetics 2020Quote: ... of which 16 reactions (200 ng in each) were amplified for 29 cycles and purified using Macherey Nagel Nucleospin Gel and PCR Purification kit (Takara Bio). Sequencing libraries were made of the 4C PCR products using Thruplex DNA-seq kit (Takara Bio) ...
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bioRxiv - Genomics 2021Quote: ... reverse transcription and cDNA preamplification were performed in the C1 Fluidigm system using the SMARTer PCR cDNA Synthesis Kit (Clontech 634926) according to manufacturer’s recommendations ...
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bioRxiv - Genetics 2020Quote: The total RNA of the two groups (control (LV) and albino mutant (HUA)) was extracted using a Plant RNA Kit (TaKaRa, Japan) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... aculeatus ovary and liver cDNA was prepared from total RNA using the SMARTer cDNA synthesis kit (Clontech, Palo Alto, CA, USA).
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bioRxiv - Microbiology 2020Quote: ... then subjected to quantitative real-time RT-PCR using a One-Step SYBR PrimeScript RT-PCR kit II (Takara Bio, Japan). The PCR mixture was mixed with 2 μl of extracted RNA and DENV-specific primer (38 ...
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bioRxiv - Microbiology 2020Quote: ... The cohesive ends of the linearized plasmid were blunt-ended and self-ligated using a DNA Blunting Kit (Code No. 6025, Takara Bio). The DNA Blunting Kit can smooth DNA with 3ʹ- or 5ʹ- protruding ends ...
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bioRxiv - Microbiology 2020Quote: ... The obtained linearized plasmid was excised from the agarose gel and purified using a MiniBEST Agarose Gel DNA Extraction Kit (Takara Bio). The cohesive ends of the linearized plasmid were blunt-ended and self-ligated using a DNA Blunting Kit (Code No ...
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bioRxiv - Microbiology 2020Quote: ... were used to transcribe poly (A)-tailed miRNAs to generate a cDNA library according to the PrimeScript™ miRNA RT-PCR Kit (Takara) instructions ...
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bioRxiv - Microbiology 2021Quote: ... PCR products to be used as DNA templates were cleaned using a NucleoSpin Gel and PCR Clean-up kit (Takara Bio). In vitro transcription was performed using MAXIscript T7 Transcription Kit (Thermo Fischer Scientific ...
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bioRxiv - Microbiology 2021Quote: ... The cDNAs were synthesized from 1 μg total RNA using the Prime Script™ RT reagent kit (Perfect Real Time; Takara). All qRT–PCR (quantitative reverse transcription–PCR ...
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bioRxiv - Immunology 2021Quote: ... RNA quality was assessed using an Agilent Bioanalyzer and 500pg of total RNA was used as input for cDNA synthesis using the Clontech SMART-Seq v4 Ultra Low Input RNA kit (Takara Bio) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... approximately 500 sorted cells were processed directly to generate cDNA using the Clontech SMART-Seq v4 Ultra Low Input Kit (Clontech, Inc.) on three biologically-independent replicates ...
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bioRxiv - Immunology 2020Quote: ... RNA was extracted and reverse transcription and cDNA amplification were performed using 10 ng of RNA using the SMARTer Ultra Low RNA kit (Clontech Laboratories) like previously reported 13 ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were cotransfected with hSyn-DsRed and the indicated construct on DIV 7 or DIV 19 (for older neurons) with 1 μg total purified plasmid DNA via CalPhos Mammalian Transfection Kit (Takara Bio) according to the manufacturer’s protocol.
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bioRxiv - Microbiology 2021Quote: ... First the coding region of Candid #1 RdRp was amplified using RNA extracted from P0 or P11 viruses and the PrimeScript II High Fidelity One Step RT-PCR Kit (Takara Bio). Kozak sequence ...
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bioRxiv - Developmental Biology 2021Quote: ... and cloned into into modified pJet:attB:mCherry vector (Roberts et al., 2014) containing phiC31 attB site and mCherry reporter using In-Fusion HD Cloning Kit (TaKaRa/Clontech) according to the manufacture instructions ...
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bioRxiv - Genomics 2021Quote: ... and reversely transcribed into cDNA according to the PrimeScript™ RT reagent Kit with gDNA Eraser (Perfect Real Time) (TaKaRa, Japan) manufacturer’s instructions ...
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bioRxiv - Genetics 2020Quote: ... Double-strand cDNA was amplified by long-distance PCR (LD-PCR) using an Advantage 2 PCR kit (Clontech, cat. no. 639206).
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bioRxiv - Immunology 2021Quote: ... The coding sequences of 14-3-3 and βTRCP were amplified by using cDNAs derived from HeLa cell as templates and inserted into vectors above using In-Fusion HD Cloning Kit (Takara Bio). For Myc-IRAK1 expression vector ...
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bioRxiv - Neuroscience 2021Quote: ... Library preparation was performed by SFGF using the SMART-Seq® v4 Ultra® Low Input RNA Kit (Takara Bio Inc). The libraries were sequenced on the Illumina HiSeq 4000 platform (2 × 75 bp paired-end reads) ...
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bioRxiv - Neuroscience 2021Quote: mRNA was purified and the cDNA was synthesized with the mRNA fragments as templates using SMART-Seq V4 RNA kit (Clontech # 634889). The sequencing libraries were generated using Nextera XT DNA Library Preparation Kit (Illumina #FC-131-1024) ...
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bioRxiv - Cancer Biology 2021Quote: ... The beads were then resuspended in a total volume of 10 µL for library preparation using the SMARTer ThruPLEX DNA-seq library preparation kit (Takara Biosciences) per manufacturer’s protocol with an adjustment on the last step ...
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bioRxiv - Microbiology 2020Quote: ... The cDNA was synthesized according to the manufacturer’s instruction of the PrimeScriptTM RT reagent Kit with the genomic DNA Eraser (Takara, Kyoto, Japan). Then ...
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bioRxiv - Neuroscience 2020Quote: ... One microgram of total RNA for each sample was reversely transcribed into cDNA by a commercial kit (RR047A, Takara Bio, Japan) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2022Quote: ... was digested using HinDIII and XhoI to remove the PSMD2 CDS and the amplified Zic3 gene was cloned using Infusion kit (Clontech, USA) as per manufacturer’s instruction. ...
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Deletion or inhibition of PTPRO mitigates diet-induced hepatic steatosis and inflammation in obesitybioRxiv - Immunology 2022Quote: ... according to the manufacturer’s instructions and reverse transcribed using the PrimeScript RT Reagent Kit with the gDNA Eraser (Takara, Shiga, Japan). To amplify cDNA ...
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bioRxiv - Cell Biology 2022Quote: All plasmid fusions assembled in-house were generated through standard ligation-based methods using the In-Fusion cloning kit according to manufacturer protocols (Takara, 638920). MBNL1 RNA-interaction and chelation mutant sequences were designed based on plasmids generated by Andrew Berglund (Purcell et al. ...
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bioRxiv - Cell Biology 2022Quote: ... Donor vectors containing the GFP or mKate2 coding sequence flanked by 1kb homology arms adopted from both 3′ and 5′ sides of the insertion site were generated by cloning of PCR-amplified tags and arms into linearized pGEM-3z vector by In-Fusion HD Cloning Kit (Takara Bio). Obtained gRNA expression plasmids and donor plasmids were injected into the y w embryos with a final concentration of 120 ng/ul for each ...