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Citations for Takara Bio :
4601 - 4650 of 5473 citations for Arg8 Vasopressin AVP ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2020Quote: ... coli MG1655 genome by colony PCR and cloned into pR6K-PAmCherry-lox71-cm-lox66 using the In-Fusion® HD cloning kit (Clontech). Mutations were introduced using the QuikChange II Site-Directed Mutagenesis Kit (Agilent) ...
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bioRxiv - Molecular Biology 2019Quote: ... PCR products were inserted into pLEX-FLAG lentiviral vector between the NotI and XhoI sites using the InFusion HD cloning kit (Takara Bio) to generate constructs with N-terminal FLAG tags ...
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bioRxiv - Cell Biology 2019Quote: ... LW2A and YKLW4A were cloned from plasmid pECerulean (37) to pHH21 with In-Fusion® HD Cloning Kit (Takara Bio, Japan). Recombinant Influenza viruses were produced with the twelve plasmids system (38 ...
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bioRxiv - Cell Biology 2019Quote: ... and PrimeScript RT reagent kit with gDNA Eraser and SYBR Premix Ex Taq II (T1i RNase H Plus) (TaKaRa, Otsu, Japan) were utilized to carry out quantitative RT-PCR (qRT-PCR ...
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bioRxiv - Molecular Biology 2019Quote: ... PCR products were gel purified (Machery Nagel) and used to generate α-32P dCTP-labeled probes using the Random Prime Labeling Kit (Takara/Clontech). Probes were purified over nucleotide purification columns (Zymo Research ...
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bioRxiv - Immunology 2019Quote: ... was added to each sample and first strand full length cDNA was generated with a modified protocol of the SMARTseq v4 Ultra Low Input RNA Kit (Takara Clontech) using poly dT primers and a template switching oligo ...
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bioRxiv - Genomics 2020Quote: ... PCRs were performed on board using 25 ng of DNA from each coral sample with the Advantage 2 kit (Takara Clontech) and a final primer concentration of 0.5 μM in a final reaction volume of 50 μl ...
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bioRxiv - Biochemistry 2020Quote: ... and ligation was used to introduce the synthetic gene in pTHT2031S In-Fusion® HD cloning kit (Takara Bio, Kusatsu, Japan). Sequencing was achieved by Genwiz (Genwiz ...
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bioRxiv - Genomics 2021Quote: ... were used for library construction using the SMARTer Stranded Total RNA-Seq Kit v.2 (634418, Pico Input Mammalian, Takara/Clontech, Japan) according to the manufacturer’s protocol without RNA fragmentation ...
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bioRxiv - Genetics 2020Quote: ... that contained both of the transgenes was subcloned to a piggyBac vector using the In-Fusion® HD Cloning Kit from Takara with the primers pNE3_UAS_to_pBac.F ...
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bioRxiv - Developmental Biology 2021Quote: ... cDNA was amplified using 8 µl of the PCR MasterMix (SMART-Seq v5 Ultra Low Input RNA Kit for Sequencing, Takara Bio) with 25 cycles of amplification ...
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bioRxiv - Developmental Biology 2021Quote: Full-length single-cell RNA-seq libraries were prepared using the SMART-Seq v5 Ultra Low Input RNA (SMARTer) Kit for Sequencing (Takara Bio). All reactions were downscaled to one-quarter of the original protocol and performed following thermal cycling manufacturer’s conditions ...
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bioRxiv - Neuroscience 2020Quote: ... pAAV backbone was cut with restriction enzymes KpnI-HF and AgeI-HF to open the backbone and oG-EGFP fragment was reinserted together with the TVA66T fragment using infusion cloning with an In-Fusion HD cloning kit (Takara bio). The plasmid was packaged into AAV8 by the SALK Viral Vector Core ...
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bioRxiv - Developmental Biology 2021Quote: 1000 FACS-purified cells from TgBAC(tcf21:H2B-Dendra2)ox182 larvae were processed using the SMART-SeqTm v4 UltraTm Low Input RNA Kit for Sequencing (Takara Clontech). Samples were lysed and poly-adenylated RNA reverse transcribed via SMARTScribe Reverse Transcriptase ...
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bioRxiv - Developmental Biology 2021Quote: ... cDNA was synthesized and amplified from 100 pg–300 pg of input RNA using SMART-seqTMv4 Ultra Low input RNA kit (Takara Clontech). Sequencing libraries were prepared using the Nextera XT DNA library preparation kit (Illumina) ...
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bioRxiv - Systems Biology 2021Quote: ... cDNA was generated from 10 ng of RNA using the SMART-Seq v4 UltraLow Input RNA Prep kit (Takara Bio Inc) per manufacturer’s suggestions ...
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bioRxiv - Biochemistry 2020Quote: ... The PfNEDD8 PCR product was cloned into the StuI-HindIII digested pSMO plasmid using in-Fusion® HD Cloning Plus kit (Takara) and transformed into DH5α E ...
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bioRxiv - Developmental Biology 2020Quote: ... All samples were prepared as RNA-seq libraries with the SMARTer Stranded Total RNA-Seq Kit v2 – Pico Input (for 250 pg – 100 ng input) (Takara Bio) with RiboZero depletion ...
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bioRxiv - Neuroscience 2021Quote: ... Reverse transcription and cDNA pre-amplification were performed using the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Clontech/Takara). cDNA was harvested and quantified with the Bioanalyzer DNA High-Sensitivity kit (Agilent Technologies) ...
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bioRxiv - Neuroscience 2021Quote: ... and cDNA was generated using the RNA to cDNA EcoDry™ Premix Oligo dT kit (Takara Bio USA, Moutain View, CA). Rin was PCR amplified using primers listed and described in Table S2 and cloned into the HindIII and EcoRI restriction sites of pAc5.1-MCS-(Gly4Ser)3-mCherry to generate the final pAc5.1:Rin-(Gly4Ser)3-mCherry vector.
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bioRxiv - Immunology 2020Quote: ... RNA quality was assessed using an Agilent Bioanalyzer and total RNA was used as input for cDNA synthesis using the Clontech SMART-Seq v4 Ultra Low Input RNA kit (Takara Bio) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... three types of the GPR151 CDS-related inserts were sub-cloned into FUGW vector using In-Fusion Cloning kit (Takara, 638910). For control experimental sets ...
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bioRxiv - Neuroscience 2021Quote: ... The cloning reaction for pSPE3::H2B-GCaMp6s was performed using linearised PCR products and the In-Fusion HD Cloning Kit (Takara Bio). H2B-GCaMP6s mRNA was produced and precipitated using the mMESSAGE mMACHINE T3 kit (Life Technologies ...
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bioRxiv - Neuroscience 2021Quote: ... First-strand cDNA synthesis was performed using the SMART-Seq v4 Ultra Low Input RNA kit (Takara Bio, Mountain View, CA) with 5 ng input RNA followed by 9 cycles of PCR amplification and library preparation using the Nextera XT DNA Library Prep Kit (Illumina ...
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bioRxiv - Neuroscience 2020Quote: ... single cells were sorted into individual wells of 8-well PCR strips containing lysis buffer from the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Takara 634894) with RNase inhibitor (0.17 U/μl) ...
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bioRxiv - Cell Biology 2021Quote: Plasmids for the pull-down assay were constructed by cloning human Kif7 and Gli2 sequences into pCMV-BICEP™-4 expression vector followed by truncations using InFusion HD Cloning Kit (Takara). Kif7 fragments were cloned at the MCS1 to express FLAG-tagged Kif7 protein and Gli2 fragments were cloned at the MCS2 to express c-myc-tagged Gli2 protein ...
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bioRxiv - Cell Biology 2021Quote: ... RNA-seq library was prepared using the SMARTer Stranded Total RNA Sample Prep kit - HI Mammalian (Takara Bio Inc, Shiga, Japan) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: The fragments containing the promoter regions of YPK1 and LCB1 were amplified by means of PCR and cloned into the XhoI and BamHI sites of the 4×CDRE-LacZ fusion plasmid, pAMS366 (38) (gifted by Dr. Martha Cyert, Stanford University) using the In-Fusion® Cloning Kit (Clontech), resulting in PYPK1-LacZ and PLCB1-LacZ reporter plasmids ...
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bioRxiv - Neuroscience 2020Quote: ... single cells were sorted into individual wells of 8-well PCR strips containing lysis buffer from the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Takara 634894) with RNase inhibitor (0.17 U/μl) ...
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bioRxiv - Neuroscience 2020Quote: ... and inserted into Bgl II/Sal I-linearized pL7 mGFP or pL7 mCherry (Wagner, McCroskery and Hammer, 2011) using an In-Fusion cloning kit (Takara, 638920). The specific fragments generated using the appropriate template and indicated primers were ...
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bioRxiv - Neuroscience 2019Quote: 2000 to 5000 frozen sorted-cells were lysed in approximatively 10 µl of lysis buffer and cDNA synthesis was performed using the SMART-Seq v4 Ultra Low Input RNA Kit (catalog 634888, Takara Bio). First-strand cDNA synthesis was performed using 20 to 50% of the input and was followed by full-length double-strand cDNA amplification using 17 PCR cycles ...
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bioRxiv - Physiology 2021Quote: ... 10 ng of obtained RNA was processed using the SMARTer Stranded RNA-seq kit for Illumina (Takara Bio Inc., Shiga, Japan) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2021Quote: ... Viral RNA from pseudovirus infected cells was also quantified by qRT-PCR using the Lenti-X qRT-PCR titration kit (Clontech; 631235) following manufacturer’s instruction ...
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bioRxiv - Plant Biology 2020Quote: ... The LightCycler 480II PCR system was programmed according to the instructions of the SYBR Premix Ex Taq II (Perfect Real Time) kit (TaKaRa, China) to conduct fluorescence-based quantitative PCR ...
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bioRxiv - Plant Biology 2020Quote: ... Total RNA was extracted using a Plant RNA Kit (R6827, Omega) and then reverse transcribed using PrimeScript™ RT Master Mix (RR036Q, Takara) per the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2021Quote: ... An aliquot of 1 μg of DNAse treated RNA was used for cDNA synthesis using the PrimeScript™ RT-PCR Kit (Takara) in a final volume of 20 μL according to the manufacturer indications ...
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bioRxiv - Plant Biology 2020Quote: ... All of the other genes encoding candidate RBOHD-associated proteins were cloned into epiGreenB5 by the same strategy using an In-Fusion HD Cloning Kit (Clontech, USA). To generate epiGreenB-pPB1CP::PB1CP-3-eGFP for transient expression in N ...
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bioRxiv - Plant Biology 2020Quote: ... and the resulting PCR product was cloned into the epiGreenB5 (3×HA) and epiGreenB(eGFP) vectors between the ClaI and BamHI restriction sites with an In-Fusion HD Cloning Kit (Clontech, USA) to generate epiGreenB5-Cauliflower mosaic virus (CaMV ...
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bioRxiv - Plant Biology 2021Quote: ... Equal quantities of RNA (1µg) were adopted for the synthesis of total cDNA using the PrimeScriptTM RT reagent kit with gDNA Eraser (TaKaRa Dalian, China). The transcript levels of CCR1 ...
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bioRxiv - Microbiology 2021Quote: ... RNAs were treated for 1 h by DNase I at 37°C and reverse-transcribed using a Prime ScriptTM RT reagent kit with gDNA Eraser (Takara, Japan). Quantitative PCR was performed as mentioned above ...
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bioRxiv - Plant Biology 2021Quote: ... followed by reverse transcription using the PrimeScript™ RT Reagent Kit with gDNA Eraser (Perfect Real Time) (Takara Bio, Shiga, Japan). Quantitative PCR was conducted using the KAPA SYBR FAST qPCR Master Mix (2X ...
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bioRxiv - Plant Biology 2021Quote: ... The full-length open reading frames of PcIBMS1 and PcIPMS1 were cloned into pTF486 between SalI and BamHI sites using In-Fusion® HD Cloning Kit (TaKaRa) following the protocol ...
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bioRxiv - Plant Biology 2021Quote: ... First-strand cDNA was synthesized from total RNA (0.5 μg) using a PrimeScript™ II 1st strand cDNA Synthesis Kit (TaKaRa, Japan). PCR was conducted with TaKaRa LA Taq® with GC buffer (TaKaRa ...
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bioRxiv - Plant Biology 2020Quote: ... was used to generate templates to synthesize first chain cDNA using the PrimeScript™ RT Master Mix kit (TAKARA, Dalian, China), with three biological replicates sample ...
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bioRxiv - Plant Biology 2020Quote: ... The recombinant plasmid was used as bait to screen the cDNA library using the Matchmaker™ Gold Yeast Two-Hybrid Library Screening System kit (Cat. no. 630489, Clontech).
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bioRxiv - Plant Biology 2019Quote: ... 2.5 kb of promoter sequences were isolated from genomic DNA and inserted upstream of the ß-glucoronidase gene of pCambia1301 vectors using the In-Fusion Cloning Recombinase kit (Clontech). The constructs were transformed into Agrobacterium tumefaciens strain GV3101 by electroporation ...
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bioRxiv - Microbiology 2019Quote: ... then 100 µL of this supernatant was used to evaluate LDH release into the culture medium as a measure of host cells lysis using a colorimetric cytotoxicity detection kit (Takara Bio) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Ad5 stocks were tittered by detection of the Ad hexon protein using Adeno-X™ Rapid Titer Kit (Cat. No. 632250, Takara, Clontech). The Ad5 CPE was dose-dependent ...
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bioRxiv - Neuroscience 2021Quote: RNA-seq libraries were prepared from 10 ng total RNA using the SMARTer Stranded Total RNA-seq Pico Input Mammalian V2 kit (Takara Bio) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... and ligated into the digested backbone of the modified pJFRC164 using the In-Fusion HD Cloning Kit (TaKaRa Clontech cat#639648) to create the plasmid 13XLexAop2-KDRT>-dSTOP-KDRT>-IVS-Syn21-GCaMP6f-p10 intermediate plasmid (named pMW02) ...