Labshake search
Citations for Takara Bio :
4851 - 4900 of 5473 citations for Arg8 Vasopressin AVP ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... the transfer plasmid (pBABEpuro-based or pLZRS-IRES-ΔNGFR/GFP-based) and pCMV-VSV-G were co-transfected into GP2-293 packaging cells using CalPhos mammalian transfection kit (Takara/Clontech) according to manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2023Quote: ... about 50 ng synthesized template DNA was in vitro transcribed using in vitro Transcription T7 Kit (for siRNA Synthesis) (Takara bio), treated with DNase I (Takara Bio) ...
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bioRxiv - Neuroscience 2023Quote: ... expressing UAS-eGFP was subjected to 14 cycles of PCR amplification (SMARTer Seq V4 Ultra Low Input RNA Kit; Takara Bio). 1 ng of amplified RNA was used to prepare cDNA libraries (Nextera XT DNA library preparation kit ...
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bioRxiv - Microbiology 2022Quote: ... The reverse transcription and the first round PCR were performed in a 25μl reaction volume by using PrimeScript™ One-Step RT-PCR Kit (TAKARA, RR055A). Cycling conditions were as follows ...
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bioRxiv - Systems Biology 2023Quote: ... 500 ng of isolated RNA was then used as input for generating cDNA and cDNA synthesis was performed using the PrimeScript RT kit (Takara Bio) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... was used as the template for real-time RT-PCR performed according to the manufacturer’s protocol using One Step TB Green PrimeScript PLUS RT-PCR kit (Takara, Cat# RR096A) and the following primers ...
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bioRxiv - Microbiology 2023Quote: ... a complete cDNA sequence of bovine hnRNPM (NM_001191223) was obtained from genomic DNA of MDBK cells using PrimeScript RT reagent Kit (TaKaRa, Shiga, Japan), followed by a PCR reaction using PrimeSTAR Max DNA Polymerase (TaKaRa ...
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Accumulation of TCR signaling from self-antigens in naive CD8 T cells mitigates early responsivenessbioRxiv - Immunology 2023Quote: ... cDNA was prepared from 1000 cell equivalent of RNA using the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Takara Bio), and next-generation sequencing libraries were generated using the Nextera XT DNA Library Preparation kit (Illumina) ...
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bioRxiv - Neuroscience 2023Quote: ... Total RNA (1–2 µg) was reverse transcribed using random hexamers and the PrimeScript™ 1st strand cDNA Synthesis Kit (TaKaRa). RT-qPCR was performed using a StepOnePlus qPCR system (Applied Biosystems ...
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bioRxiv - Plant Biology 2023Quote: Plasmids for targeted gene deletion by homologous recombination were assembled using the In-Fusion® HD Cloning Kit (Takara Bio, Japan). Briefly ...
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bioRxiv - Cancer Biology 2023Quote: ... Where possible equivalent amounts of total RNA (100pg) from all samples were used for first strand synthesis with the SmartERv3 kit (Takara Clontech) followed by 15-18 cycles of amplification (according to manufacturers’ instruction) ...
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bioRxiv - Microbiology 2022Quote: ... was used as the template for real-time RT-PCR performed according to the manufacturer’s protocol using One Step TB Green PrimeScript PLUS RT-PCR kit (Takara, Cat# RR096A) and the following primers ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... All PCR purifications were performed using the NucleoSpin® Gel and PCR Clean-up kit (TAKARA Bio Inc., San Jose, CA) according to manufacturer protocols ...
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bioRxiv - Biochemistry 2023Quote: ... Total RNA was further purified using mRNA Dynabeads and the purified mRNA was used as template for synthesis of cDNA using SMARTer PCR cDNA kit (Takara-Bio), with 18 cycles of amplification ...
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bioRxiv - Microbiology 2022Quote: ... RNA samples with sufficient material (10 pg–10 ng) were passed to whole-transcriptome library preparation using the SMART-Seq v4 PLUS Kit (Takara Bio) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: Sample preparation for RT-qPCR from primary cultured neurons was performed using the RNeasy Mini Kit and PrimeScript RT Master Mix (Takara Bio). RT-qPCR was performed using KOD SYBR qPCR Mix (TOYOBO ...
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bioRxiv - Molecular Biology 2023Quote: ... the total RNA of three samples in each group was extracted with the TaKaRa MiniBEST Universal RNA Extraction Kit (TaKaRa, Japan), and linear RNA was then removed with Rnase R (GENESEED ...
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bioRxiv - Cell Biology 2023Quote: ... and primers containing approximately 500 base pairs sequence homologous to the target locus and then cloned into the EcoRV site of pBluescript SK+ using an In-Fusion HD Cloning Kit (Takara Bio). TP53-missense mutations and protospacer adjacent motif (PAM ...
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bioRxiv - Biochemistry 2023Quote: ... 1,5 µg of plasmid DNA and 1,5 µg pOG44 (encoding the Flp recombinase) were mixed and transfected using CalPhos Mammalian Transfection Kit (Clontech®/Takara, #631312). The transfection medium was replaced by complete DMEM after 24 h ...
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bioRxiv - Cell Biology 2023Quote: ... An amount of 1 μg RNA was reverse transcribed into cDNA using the PrimeScript RT Reagent Kit (Takara Biomedical Technology, China). For RT-qPCR ...
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bioRxiv - Developmental Biology 2023Quote: ... Reverse transcription and cDNA library preparation were performed with a SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Clontech, 634888). cDNA was fragmented with a Covaris S 220 instrument (Covaris) ...
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bioRxiv - Cell Biology 2023Quote: ... cDNA libraries were prepared using a SMARTer® Stranded Total RNA-Seq Pico Input Mammalian kit v2 (Takara Bio, Shiga, Japan), and sequencing was completed using a HiSeq 2500 System (Illumina ...
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bioRxiv - Developmental Biology 2023Quote: ... ATAC-identified enhancers were inserted into the ZED vector by In-Fusion cloning (Cat. #638910, In-Fusion HD Cloning Plus Kits, Takara Bio) using BspEI and BmgBI cutting sites for linearisation ...
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bioRxiv - Developmental Biology 2023Quote: ... lentivirus was first packaged following protocols we described previously1 and was then concentrated 100x using Lenti-X Concentrator kit (Takara, # 631231). Concentrated lentivirus was aliquoted and stored in -80°C before use ...
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bioRxiv - Plant Biology 2023Quote: ... and inserted into the Aor51HI-linearized pMpGWB123/223/323/423 vectors using In-Fusion HD Cloning Kit (Takara Bio, Shiga, Japan).
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bioRxiv - Plant Biology 2023Quote: ... Each sample of total RNA (1 mg) was reverse transcribed by the PrimeScriptTM RT reagent Kit with gDNA Eraser (cat no. RR047A, Takara, Japan). The resistance genes examined were the same as those investigated in our previous study 9.
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bioRxiv - Cancer Biology 2023Quote: ... iCaspase-T2A-tdTomato sequence was removed and swapped for mNeonGreen-NLS using a combination of Q5® High-Fidelity DNA Polymerase and the In-Fusion HD Cloning kit (Takara). Final targeting vector was generated by insertion of homology arms (∼600bp each ...
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bioRxiv - Zoology 2023Quote: ... First- strand cDNA was synthesized from total RNA using the PrimeScript RT Reagent Kit with gDNA Eraser (Takara Bio, Shiga, Japan). PCR amplifications were performed using the KOD One PCR Master Mix (TOYOBO ...
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bioRxiv - Microbiology 2023Quote: ... was used as the template for real-time RT-PCR performed according to the manufacturer’s protocol using the OneStep TB Green PrimeScript PLUS RT-PCR kit (TAKARA, Cat# RR096A) and the following primers ...
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bioRxiv - Genomics 2023Quote: ... purified via gel extraction from a 1% agarose gel followed by cleanup using the NucleoSpin PCR clean up and gel extraction kit (Takara Bio). Linearized CROP-seq-optiI and amplified oligonucleotides were assembled using the NEBuilder HiFi DNA assembly cloning kit (NEB ...
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bioRxiv - Molecular Biology 2023Quote: ... A total of 1 μg of total RNA was reverse-transcribed with random primers using the PrimeScript™ 1st strand cDNA Synthesis Kit (TaKaRa) according to the manufacturer’s protocols ...
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bioRxiv - Developmental Biology 2023Quote: ... was eliminated by digesting with genomic DNA eraser buffer and cDNA was obtained by reverse transcription of RNA using PrimeScript RT reagent Kit with gDNA Eraser (Takara, RR047A). To measure the values of genes specifc primers ...
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bioRxiv - Microbiology 2023Quote: ... TwistAmp® Basic Kit (TwistDx, TABAS03KIT), Milenia HybriDtect1 (TwistDX, Milenia01), UltraPure DNase/RNase-Free Distilled Water (Thermo, 10977015) and RNase inhibitor (Takara, 2313A). Thermo Fisher Nanodrop 1000 Spectrophotom was used to quantify nucleic acid ...
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bioRxiv - Molecular Biology 2023Quote: ... All the mutant constructs of CE were prepared from pcDNA4/TO-bio-myc-mCE by using In-Fusion Site Directed Mutagenesis kit (Takara Bio). mCherry-H2B ...
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bioRxiv - Immunology 2023Quote: ... total RNA (1 ng) was injected into the SMART-Seq v4 Ultra Low Input RNA Kit (Clontech, Palo Alto, CA, USA) for sequencing ...
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bioRxiv - Plant Biology 2023Quote: ... were converted to cDNA with oligo dT primers according to the instructions of the PrimeScript RT reagent Kit with gDNA Eraser (Takara, Japan). Quantitative PCR was performed in accordance with manufacturer’s instructions for THUNDERBIRD SYBR qPCR Mix (TOYOBO ...
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bioRxiv - Microbiology 2023Quote: ... was used as the template for real-time RT-PCR performed according to the manufacturer’s protocol using a One Step TB Green PrimeScript PLUS RT-PCR kit (Takara, Cat# RR096A) and the following primers ...
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bioRxiv - Biochemistry 2023Quote: TssM193-490 coding region was obtained by gene synthesis (IDT) and cloned into pOPIN-K vector45 using the In-Fusion HD Cloning Kit (Takara Clontech). Point mutations were introduced using the QuikChange Lightning kit (Agilent Technologies).
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bioRxiv - Cancer Biology 2022Quote: Sequencing libraries were prepared from RNA using the SMARTer Stranded Total RNA-Seq Kit v3 for picogram input material (Takara, #634488). Libraries were sequenced as paired-end 101 bp (tumoroid samples ...
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bioRxiv - Microbiology 2023Quote: ... RNA samples with sufficient material (10 pg–10 ng) were passed to whole-transcriptome library preparation using the SMART-Seq v4 PLUS Kit (Takara Bio) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... and the PCR products were subcloned into the pcDNA3.1(+) vector (digested with KpnI/BamHI) using an In-Fusion HD Cloning Kit (#Z9648N; TaKaRa, Kusatsu, Japan), resulting in pcDNA3.1(+ ...
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bioRxiv - Cell Biology 2022Quote: ... and the PCR product was subcloned into the pcDNA3.1(+) Ms Jaw1 PA (digested with XhoI) using an In-Fusion HD Cloning Kit (#Z9648N; TaKaRa, Kusatsu, Japan), resulting in pcDNA3.1(+ ...
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bioRxiv - Cell Biology 2022Quote: ... C-terminal PA vector was first prepared by insertion of a DNA cassette encoding the PA tag (annealed primer set 28) into the pcDNA3.1(+) vector (digested with XhoI/XbaI) using a DNA Ligation Kit (#6023; TaKaRa, Kusatsu, Japan). The DNA fragment coding for Ms Jaw1 was then amplified by PCR with primer set 29 from pTagGFP2-C Ms Jaw1 (previously described by Kozono et al. ...
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bioRxiv - Developmental Biology 2023Quote: ... Full length cDNA was generated using Clontech SMART-Seq v4 Ultra Low Input RNA kit for Sequencing (Takara Bio Europe, 634891) according to manufacturer’s instructions with 15 cycles of PCR for coding DMA (cDNA ...
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bioRxiv - Genetics 2023Quote: ... the two PCR fragments could be assembled and cloned into the SacII-NotI site of pPGKneo-F2F using the In-Fusion Cloning HD Kit (Takara; 639648), resulting in the generation of pPGKneo-F2F-Klf2-5HR ...
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bioRxiv - Immunology 2023Quote: ... and then ten nanograms of total RNA was used as input for cDNA synthesis using the Clontech SMART-Seq v4 Ultra Low Input RNA kit (Takara Bio) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... A total of 100 ng isolated RNA was used for cDNA synthesis using PrimeScript High Fidelity RT-PCR Kit (Takara, R022B). RT-qPCR was performed using TB Green® Premix Ex Taq™ (Tli RNase H Plus ...
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bioRxiv - Genomics 2023Quote: ... Primers were designed with around 15 bp of overlapping sequence to ensure proper circularization with the In-Fusion HD cloning plus kit (Takara #638909). Mach1 competent cells (Thermo Fisher Scientific #C862003 ...
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bioRxiv - Systems Biology 2023Quote: ... Genomic DNA was extracted from cell pellets using the Machery Nagel NucleoSpin Blood Kit (Midi, Clontech Cat. #740954.20; XL Maxi, Clontech Cat. #740950). The detailed protocol is available at http://www.broadinstitute.org/rnai/public/resources/protocols.
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bioRxiv - Plant Biology 2023Quote: ... Each sample (2 μg) of total RNA was used to synthesize first-strand cDNA with a PrimeScript RT Kit with gDNA eraser (Takara, RR0447A). RT-qPCR was performed using a Roche LightCycler 480 system with a SYBR Fast qPCR Kit (KAPA Biosystems ...