Labshake search
Citations for Takara Bio :
4551 - 4600 of 5356 citations for Pig Glucagon Like Peptide 2 GLP 2 CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... zroraa LBD deletion DN -: 5′- ctgattatgatctagagtccaggccggattgatcagg-3 and inserted into a Tol2-lyzC-mcherry-2A backbone by using infusion cloning kit (Takara #638920). The construction method for neutrophil-specific Cas9 expression and the guide RNA expression fish lines has been described in our previous study [40] ...
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bioRxiv - Cell Biology 2020Quote: ... putative miR-31-5p binding sequences were deleted in the plasmid DNA using PrimeSTAR Mutagenesis Basal Kit (TaKaRa Bio, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... Tcrb amplicons were prepared using a 5’RACE-based protocol with the SMARTer Mouse TCR α/β Profiling Kit (Takara #634402) following the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Cells cultured in either 12 or 24-well plates were washed twice with cold PBS and harvested using TaKaRa MiniBEST Universal RNA extraction kit (Takara, Japan). RNA was purified using the same kit according to manufacturer’s protocol ...
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bioRxiv - Neuroscience 2020Quote: 800pg total RNA from each sample pool was subjected to library construction according to manufacturer’s instructions (SMARTer® Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian, Takara Bio). Libraries were barcoded ...
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bioRxiv - Microbiology 2021Quote: ... was used as the template for real-time RT-PCR performed according to the manufacturer’s protocol using the One Step TB Green PrimeScript PLUS RT-PCR kit (Takara, cat# RR096A) and the following primers ...
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bioRxiv - Immunology 2021Quote: ... and the reverse transcription products were amplified using the Agilent-Strata gene MxReal-Time qPCR system with a PrimeScript™ RT Reagent Kit (Takara). Reverse transcription products were amplified using an Agilent-Strata gene MxReal-Time qPCR system with TB Green® Premix Ex Taq™ II (Tli RNaseH Plus ...
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bioRxiv - Microbiology 2021Quote: ... The amplified fragments were cloned into the SmaI site of pPTR I using an In-Fusion HD cloning kit (Takara Bio). Transformants were selected on minimal agar medium with pyrithiamine.
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bioRxiv - Microbiology 2021Quote: ... The amplified fragment was cloned into the SmaI site of pPTR I using an In-Fusion HD cloning kit (Takara Bio). The pPTR I-GFP-nrdA plasmid was used to transform the A ...
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bioRxiv - Microbiology 2021Quote: ... The amplified cDNAs were cloned into the indicated plasmids using an In-Fusion HD cloning kit (Clontech, Mountain View, CA, USA). The sequences of all plasmids were confirmed by Eurofins Genomics (Tokyo ...
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bioRxiv - Bioengineering 2020Quote: ... 50 ng of total RNA from each sample was amplified and synthesised to cDNA with SMART-seq (SMART-Seq v4 Ultra Low Input RNA Kit, Takara Bio). Then ...
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bioRxiv - Bioengineering 2020Quote: ... Viral vectors were purified using an AAVpro® Purification Kit (All Serotypes) (catalog no. 6666; Clontech Laboratories, Mountain View, CA, USA) 48 hours after viral transduction ...
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bioRxiv - Molecular Biology 2022Quote: ... Equal amounts of RNA samples (200 ng) were used to generate complementary DNA using a PrimeScript RT reagent kit (TaKaRa Bio) with oligo-dT and random primers ...
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bioRxiv - Microbiology 2022Quote: ... NIH) and then cloned into pLVX-IRES-zsGreen1 vector using an In-Fusion HD cloning kit (Clontech, Mountain View, CA, USA). The GAD mutant of ORF1 inactivating the polymerase was generated by QuikChange (Stratagene ...
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bioRxiv - Microbiology 2022Quote: ... All DNA fragments were cloned into the respective vectors using an In-Fusion HD cloning kit (Clontech, Mountain View, CA, USA). All constructs or primers used to construct the HEV reporter genomes in Supplementary Table 1 have been validated through Sanger sequencing and are available upon request.
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bioRxiv - Genomics 2022Quote: ... 1.51 million bacterial colonies were collected and the plasmids were extracted with NucleoBond Xtra Maxi EF kit (Takara Bio USA #740424). To insert a TmU DNA fragment containing the tracRNA (E/F ...
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bioRxiv - Genomics 2022Quote: ... Total RNA from SARS-CoV-2 infected lung or trachea was subjected to one-step real-time reverse transcription PCR using One-step PrimeScript RT-PCR kit (Takara, RR064B). Multiplex PCR was performed to detect SARS-CoV-2 nucleocapsid gene and mouse Actb gene ...
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bioRxiv - Molecular Biology 2022Quote: ... genes were cloned into a His-SUMO fusion protein expression vector (Kim et al, 2018) using an In-Fusion Cloning Kit (639648, Takara Bio). The glycine-arginine-proline (GRP ...
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bioRxiv - Neuroscience 2019Quote: ... 1 μg total RNA was reverse transcribed into cDNA using the PrimeScriptTM RT reagent kit with gDNA Eraser (Perfect Real Time) (TaKaRa, Japan) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... The bacterial RNA was reverse transcribed into complementary DNA (cDNA) using the PrimeScript RT reagent Kit (Perfect Real Time) (Takara: RR037A) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... TUNEL assay was performed to detect apoptotic cell death using the in situ Apoptosis Detection Kit (Takara Bio Inc., Shiga, Japan) according to the manufacturer’s instructions ...
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Biosynthesis of Circular RNA ciRS-7/CDR1as Is Mediated by Mammalian-Wide Interspersed Repeats (MIRs)bioRxiv - Molecular Biology 2019Quote: ... The expression plasmids for circSPNS1 was constructed by subcloning the PCR-amplified fragments into FLAG-pcDNA3 plasmid using an In-Fusion HD cloning kit (Takara Bio) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2019Quote: ... The final PCR fragments were cloned into the XhoI/KpnI restriction sites of the pARL-STEVORfull vector backbone60 using the In-Fusion HD cloning kit (Clontech # 639649). The resulting plasmids were verified by Sanger sequencing ...
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bioRxiv - Molecular Biology 2019Quote: ... and cloned into the BtgZ1 restriction site of the pL6-egfp plasmid as described elsewhere61 using the In-Fusion HD cloning kit (Clontech # 639649). For the gControl gRNA ...
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bioRxiv - Genetics 2019Quote: ... About 1 μL of viral RNAs were used as template to synthesize cDNAs with PrimeScript™ RT reagent Kit with gDNA Eraser (TaKaRa). Absolute quantitative real-time PCR assay was performed with SYBR green I (TOYOBO ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... The GFP coding sequence was ligated to the 3’ end of the SLC22A24 ortholog cDNA in the expression vector pcDNA5/FRT using In-Fusion HD cloning kit (ClonTech #639642). The human SLC22A24 GFP fusion construct was purchased from OriGene (Catalog number ...
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bioRxiv - Plant Biology 2019Quote: ... and the Gal4 AD fused with SV40 large T-antigen (pGADT7-T) from the Matchmaker Gold Yeast Two-Hybrid System kit (Takara Bio) were used as positive control ...
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bioRxiv - Plant Biology 2019Quote: ... The yeast containing Gal4 DNA-BD fused with lamin (pGBKT7-Lam) and the pGADT7-T from the Matchmaker Gold Yeast Two-Hybrid System kit (Takara Bio) were used as a negative control.
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bioRxiv - Neuroscience 2019Quote: ... HeLa cells were transfected with 3 μg of the designated construct or empty plasmid (see below) using the CalPhos transfection kit (Clontech, 631312). HeLa cells were used 48 h after transfection.
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bioRxiv - Animal Behavior and Cognition 2019Quote: ... were designed to amplify the internal region of 11β-h and 11β-hsd2 gene respectively by using Takara PCR Amplification Kit (Takara, Japan). To obtain the full-length cDNA sequences ...
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bioRxiv - Developmental Biology 2020Quote: ... 700pg of total RNA were used for cDNA amplification with the SMART-Seq v4 Ultra Low Input RNA Kit (Clontech-Takara). 1 ng of amplified cDNA was used to generate barcoded libraries using the Nextera XT DNA library preparation kit (Illumina) ...
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bioRxiv - Physiology 2020Quote: ... Double-stranded cDNA libraries (RNA-seq libraries) were prepared using SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Cat. No. 634888, Takara Bio) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... from each ample was used to synthesize first-strand cDNA by reverse transcription using the PrimeScript™ RT reagent Kit with gDNA Eraser (Takara), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: The 5’ and 3’ RACE analyses were performed according to the protocol for the SMARTer® RACE 5’/3’ Kit (TaKaRa Bio USA ...
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bioRxiv - Cell Biology 2020Quote: ... Double-stranded cDNA libraries (RNA-seq libraries) were prepared using SMARTer® Stranded Total RNA-Seq Kit v2 – Pico Input Mammalian (Clontech) according to the manufacturer’s instruction ...
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Combinations of maternal-specific repressive epigenetic marks in the endosperm control seed dormancybioRxiv - Molecular Biology 2020Quote: ... and the REF6 coding sequence was inserted into the AatII and Eco32I sites using the In-Fusion® HD Cloning Kit (TaKaRa).
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bioRxiv - Plant Biology 2020Quote: cDNA was produced in triplicates from three μg of RNA by prime script 1st strand cDNA synthesis kit (Takara-cat # 6110A). Gene expression was scrutinized in reverse transcription-quantitative polymerase chain reaction (RT-qPCR ...
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bioRxiv - Cell Biology 2020Quote: ... Full-length Nrg cDNA obtained from DGRC (clone GH03573) was inserted into the linearized backbone (EcoRI, SfiI) using an In-Fusion HD Cloning Kit (Takara Bio). Site-specific attp40 insertion into the fly genome was performed by Bestgene ...
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bioRxiv - Developmental Biology 2020Quote: ... or into a 200 μl tube containing 5 μl of lysis buffer and 5% RNase inhibitor (for scRNA-seq) (lysis buffer and RNase inhibitor from SMART-Seq v4 Ultra Low Input RNA Kit, Takara, # 634892). Once the dissociation process began ...
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bioRxiv - Developmental Biology 2021Quote: ... and limited cDNA amplification was performed using the SMARTer Ultra Low Input RNA Kit for Sequencing – v4 (Takara Bio USA, Inc.). The resulting full-length cDNA was fragmented and tagged ...
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bioRxiv - Synthetic Biology 2021Quote: ... Aliquots of 1 μg of the treated RNA were used for cDNA synthesis with oligo dT using PrimeScript™ RT-PCR kit (Takara). cDNAs (0.4 μl ...
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bioRxiv - Molecular Biology 2020Quote: ... Illumina data from human HEK293T cells were processed with the SMARTer® smRNA-Seq Kit for Illumina (Takara, Cat. Nos. 635029) following guidelines ...
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bioRxiv - Molecular Biology 2020Quote: ... A full-length cDNA library was constructed for each sample using the TeloPrime Full-Length cDNA Amplification Kit V1 (Lexogen) and amplified using PrimeSTAR GXL DNA Polymerase (Takara Bio) with 22 PCR cycles of 98 °C denaturation for 10 seconds ...
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bioRxiv - Neuroscience 2021Quote: ... RNA samples were reverse transcribed into cDNA and amplified using Smartseq v4 Ultra Low Input RNA Kit for Sequencing (Takara #634889), per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... RNA quality was assessed using an Agilent Bioanalyzer and ten nanograms of total RNA used as input for library preparation using the SMARTer Stranded Total RNA-Seq V2 Pico Input Mammalian kit (Takara Bio) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2020Quote: ... The full-length cDNA of the NIC1-locus ERF genes were amplified using PrimeScript First-Strand cDNA Synthesis Super Mix Kit (Takara, Japan) from the first-strand cDNA with gene-specific primers with suitable restriction enzyme sites (Additional file 3 ...
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bioRxiv - Molecular Biology 2021Quote: ... cDNA was reverse-transcribed from 800 ng of total RNA using the PrimeScript™ RT reagent Kit with gDNA Eraser (Takara). Gene-specific primers with appropriate amplification efficiency (0.95–1.05 ...
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bioRxiv - Plant Biology 2021Quote: ... 1 μg of the total RNA from the leaves was reverse-transcribed for 30 min at 42°C in a 20-μl reaction volume using a High Fidelity PrimeScriptTM RT-PCR kit (Takara, Japan) according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2021Quote: ... which contains a six-amino acid His-tag for the creation of a N-terminal fusion using the In-Fusion HD cloning kit (Clontech, USA).
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bioRxiv - Developmental Biology 2019Quote: ... and subjected to direct cDNA synthesis using the SMART-Seq v4 ultra Low Input RNA Kit for Sequencing (Clontech Laboratories #634888). Approximately 250pg of cDNA was used for preparation of dual-indexed libraries using the Nextera XT DNA Library Preparation Kit (Illumina # FC-131-1002 ...