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Citations for Takara Bio :
4401 - 4450 of 5356 citations for Pig Glucagon Like Peptide 2 GLP 2 CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2024Quote: ... esrp1 and esrp2 were each cloned into a pCS2+8 plasmid backbone using the In-Fusion HD Cloning Kit (Clontech). The resulting pCS2+8-esrp2 plasmid was mutagenized with synonymous mutations surrounding the translational start-site using the GeneArt site-directed mutagenesis (SDM ...
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bioRxiv - Synthetic Biology 2024Quote: ... Ribo-seq RNA libraries were prepared for sequencing using the SMARTer® smRNA-Seq Kit for Illumina (#635030) from Takara Bio USA ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Libraries were harvested by scraping colonies from transformation plates and directly purifying plasmids (with no outgrowth that could introduce amplification bias) using Nucleobond Xtra midiprep kits (Takara).
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bioRxiv - Genomics 2024Quote: ... Alu6B and Aluh1.1 were synthesized as gene blocks (IDT) and cloned into BamHI-AatII sites in the pYa5-neo vector using Infusion cloning kit (Takara) following the manufacturer’s protocol ...
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bioRxiv - Genetics 2024Quote: ... ∼0.37 pg) per tube—was subjected to amplification using a PicoPLEX® Gold Single Cell DNA-Seq Kit (Takara Bio) and indexing primers (DNA HT Dual Index kit ...
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bioRxiv - Plant Biology 2024Quote: ... respectively) were inserted into the KpnI and XbaI sites of the vector using the In-Fusion HD Cloning Kit (TaKaRa).
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bioRxiv - Immunology 2024Quote: ... 100ng of total RNA was used for cDNA synthesis by RNA to cDNA EcoDry™ Premix (Double Primed) kit (Takara), and qPCR was performed by Taqman primers with TaqMan™ Fast Advanced Master Mix (Applied Biosystems™ ...
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bioRxiv - Bioengineering 2024Quote: ... The rituximab and cetuximab antibody variable sequences were generated by IDT and cloned into the variable regions of the VRC01 antibody plasmid vector (a generous gift from the Kim lab at Stanford) by using the In-Fusion cloning kit (Takara) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... cDNA synthesis and library preparations were performed by the Genomics Core using the SMARTv4 for ultra-low RNA input and Nextera XT kits (Takara). Sequencing was also performed on an Illumina NextSeq 2000 sequencing system with paired-end ...
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bioRxiv - Cell Biology 2024Quote: ... was inserted into pcDNA5-FRT/TO to generate pcDNA/TO/Dyn1-K44A-GFP using the In-Fusion cloning kit (Takara), and the following primers ...
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bioRxiv - Cell Biology 2024Quote: cfDNA in the conditioned cell culture medium was extracted using NucleoSpin Gel and PCR Clean-up kit (Takara, Duren, Germany), and quantified with Nanodrop (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... The cells were grown for 72 hours post-transfection and the virus was concentrated ∼40-fold from the supernatant using the Lenti-X concentrator kit (Takara) per the manufacturer’s protocol ...
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bioRxiv - Biophysics 2024Quote: ... The concentration of purified proteins was determined by the Bradford method using TaKaRa Bradford Protein Assay Kit (Takara Bio, T9310A).
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bioRxiv - Bioengineering 2024Quote: ... The purified PCR product was cloned with a C-terminus 6-His tag into pET22b vector via NdeI/XhoI using the In-Fusion HD Cloning kit following the manufacturer’s protocol (Takara Bio). The construct was transformed into chemically competent E ...
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bioRxiv - Microbiology 2024Quote: ... The quantified 400 ng of RNA used for cDNA preparation using the PrimeScript 1st strand cDNA Synthesis Kit (Takara Bio). The forward and reverse primers used for amplification are used as previously described (32) ...
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bioRxiv - Microbiology 2024Quote: ... Then the total RNA was extracted and reversed transcribed into cDNA for quantitative PCR by using the PrimeScript RT Reagent Kit (TaKaRa). Quantitative real-time reverse transcriptase PCR (qRT-PCR ...
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bioRxiv - Developmental Biology 2024Quote: ... Sample tubes were reverse transcribed and amplified using the SMART-Seq V4 Ultra Low Input RNA Kit (Takara Bio 634891) in 10x reduced volume reactions using a Mantis liquid handling system (Lee et al ...
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bioRxiv - Molecular Biology 2024Quote: ... and the insert fragments fused to the linearized plasmids using the In-Fusion Cloning Kit (Takara Bio Inc., Shiga, Japan), according to the protocol provided by the manufacturer with the Escherichia coli JM109 strain ...
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bioRxiv - Developmental Biology 2024Quote: ... quantitative real-time PCR (RT-qPCR) was performed using the cDNA libraries as the template and a TB Green Premix Ex Taq kit (Takara) in a CFX96 real-time PCR machine (BioRad) ...
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bioRxiv - Cell Biology 2024Quote: ... 4F2hc and mZIP13 or mZIP14 were fused by PCR and In-Fusion® HD Cloning Kit (Takara Bio, Shiga, Japan), according to the manufacturer’s instructions ...
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CTCF regulates global chromatin accessibility and transcription during rod photoreceptor developmentbioRxiv - Genomics 2024Quote: ... and 1 ng total RNA was used to generate RNA-seq libraries with SMARTer Stranded Total RNA-Seq Kit v3 (Takara). All samples were sequenced with NovaSeq 6000 (Illumina ...
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bioRxiv - Developmental Biology 2024Quote: ... amounts ranging from 10 picograms to 10 nanograms of total RNA were employed using the SMART-seq v4 Ultra Low Input RNA Kit from Takara Bio ...
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bioRxiv - Genetics 2024Quote: ... Esrp1 cDNA was cloned into the pcDNA3.1 backbone containing a CMV promoter and SV40 polyA tailing sequence for expression in mammalian cells using the In-Fusion HD Cloning Kit (Clontech) to generate the pcDNA3.1-esrp1-mCherry plasmid ...
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bioRxiv - Synthetic Biology 2024Quote: ... cells were collected and AAV6 capsids were isolated using the AAVPro Purification Kit (All Serotypes, Takara Bio, San Jose, USA), as per the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... total RNA (0.5 ng) was added to reaction buffer from the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Takara), and reverse transcription was performed followed by PCR amplification to generate full length amplified cDNA ...
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bioRxiv - Developmental Biology 2024Quote: ... then we reversed transcription of these RNA to create cDNA by a PrimeScript 1st strand cDNA synthesis kit (Takara, Japan). FMNL2-EGFP vector was generated by Wuhan GeneCreate Biological Engineering Co ...
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bioRxiv - Cell Biology 2024Quote: ... the RNA was reverse transcribed into complementary DNA by PrimeScript RT (reverse transcriptase) using a primeScript RT-PCR kit (Takara), and qPCR was performed with SYBR Green-Master Mix (ThermoFisher Scientific ...
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bioRxiv - Systems Biology 2024Quote: ... in conjunction with the Mir-X™ miRNA qRT-PCR TB Green® Kit from TAKARA (San Jose, CA, USA). After confirming miRNA inhibition in tick tissues ...
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bioRxiv - Genomics 2024Quote: ... 10 ng of total RNA was amplified and converted into cDNA using SMART-Seq v4 Ultra Low Input RNA kit (Clontech). About 17 fmol of cDNA was used for library preparation using the PCR Barcoding kit (SQK-PBK004 kit ...
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bioRxiv - Plant Biology 2020Quote: Total RNA was extracted from plant samples with Purelink (Nacalai Tesque, Japan) and reverse transcribed with PrimeScript Reagent Kit Perfect Real Time (Takara, Japan) according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2020Quote: ... The yeast one-hybrid (Y1H) assay was conducted using the Matchmaker™ Gold Yeast One-Hybrid Library Screening System kit (Cat. no. 630491, Clontech).
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bioRxiv - Plant Biology 2019Quote: ... One microgram of DNA-free RNA was used to synthesize cDNA by using PrimeScriptTM RT Reagent Kit (TAKARA, Kusatsu, Shiga, Japan).
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bioRxiv - Plant Biology 2019Quote: ... 1 μg DNase I-treated RNA was used for synthesizing the first strand cDNA according to the manufacturer’s instructions (PrimeScript II 1st Strand cDNA Synthesis Kit; Takara, Dalian, China). The primers of EcaICE1 and EcaSIZ1 (listed as table 1 ...
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bioRxiv - Biophysics 2021Quote: ... RNA was synthetized using the T7-Scribe transcription kit (Cellscript) and purified with Chroma Spin DEPC-H2O columns (Clontech, CA, USA). The ncAA naphthalene was ligated to the tRNA with T4 DNA ligase (New England Biolabs ...
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bioRxiv - Microbiology 2020Quote: ... HSP70-F2/R2 primers (Table S1) and ligated into pBS-SK+ using the In-fusion HD Cloning kit (Clontech, Beijing, China), respectively ...
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bioRxiv - Cancer Biology 2021Quote: ... and quantitative real-time PCR was performed using the SYBR® Premix Ex Taq II Reagent Kit (Takara, Cat. Cat. #RR820A) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... PCR amplified fragments and linearized vector by restriction enzyme digestion were assembled using In-Fusion HD Cloning Kit (Takara Bio Inc.). pEGFPN3-ARL4C was constructed as previously described(Matsumoto et al ...
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bioRxiv - Cell Biology 2020Quote: ... 10ng of each RNA isolate was then prepared using the Takara SMARTer Stranded Total RNA-Seq Kit v2 – Pico Input Mammalian (Takara, 634412). Transcripts were fragmented for 3.5 minutes and amplified for 12 cycles ...
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bioRxiv - Immunology 2021Quote: ... DNase-treated total RNA was used to prepare cDNA libraries with the SMARTer smRNA-Seq Kit (Takara Bio, Mountain View, CA). Libraries were sequenced as 50 bp single-end reads on an Illumina HiSeq sequencer ...
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bioRxiv - Genetics 2021Quote: ... The cDNA samples were prepared from 1 μL total RNA per reaction using a reverse transcription kit (Takara Biotechnology, Tokyo, Japan) and stored at - 20°C for qRT-PCR assays ...
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bioRxiv - Genetics 2021Quote: Adult ovary total RNA was used to amplify the 5’ and 3’ UTR of Pxmeiw68 and PxnanosP using the SMARTer RACE 5’/3’ Kit (Takara, Japan). 5’ and 3’ regulatory sequences were then cloned from 4th instar larval gDNA ...
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bioRxiv - Developmental Biology 2021Quote: ... three gene-specific reverse primers were designed and a nested PCR program was used according to the protocol of a genome walking kit (Takara, Japan). The sequences of the primers used for amplification are shown in Table S1 ...
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bioRxiv - Developmental Biology 2021Quote: ... PCR donor amplicons were purified using the Nucleospin Gel and PCR Clean-up kit (Machery-Nagel, distributed by Takara Bio USA). PCR products were not gel purified ...
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bioRxiv - Developmental Biology 2021Quote: ... and 1 ng of RNA was used for cDNA synthesis (SMART-Seq v4 Ultra Low Input RNA kit – Takara Bio: #634898). Verification of library quality and sequencing were done as for the embryonic dataset (see above) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and was converted into cDNA prior to sequencing using the SMARTer technology (SMARTer Pico PCR cDNA Synthesis Kit, Takara, cat. # 634928). Sequencing libraries were prepared using the Illumina TruSeq protocol and sequenced on an Illumina MiSeq using 250 bp paired end reads ...
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Three Distinct Transcriptional Profiles of Monocytes Associate with Disease Activity in SSc PatientsbioRxiv - Genomics 2022Quote: ... Full-length RNA-seq libraries from CM and skin macrophages were prepared from ∼3 ng of total RNA using SMART-Seq v4 Ultra Low Input RNA Kit (Clontech Laboratories) followed by Nextera XT protocol (Illumina) ...
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bioRxiv - Molecular Biology 2022Quote: ... The amplified fragments were purified using a gel extraction kit (Vazyme, China) and cloned into a pMD-18T vector (Takara, Japan).
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bioRxiv - Cell Biology 2022Quote: ... The gene encoding TERT and mVenus was inserted into pSBbi vector using the In-Fusion cloning kit (Takara Bio, Shiga, Japan). The R3E/R6E mutation was introduced using the KOD mutagenesis kit (Toyobo ...
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bioRxiv - Molecular Biology 2020Quote: ... the genome of Pv11 cells and the clonal cell lines were extracted with a NucleoSpin Tissue kit (Takara Bio, Shiga, Japan) and subjected to PCR using the following primer set ...
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bioRxiv - Molecular Biology 2020Quote: ... Single cell polyA[+] RNA-seq on the C1 was performed using the SMART-Seq v4 Ultra Low Input RNA Kit for the Fluidigm C1 System (Takara, 635026) according to manufacturer’s instructions ...