Labshake search
Citations for Takara Bio :
4801 - 4850 of 5356 citations for Pig Glucagon Like Peptide 2 GLP 2 CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... The RNA was then reverse-transcribed into complementary DNA (cDNA) using the RR036A PrimeScript™ RT Reagent Kit (Perfect Real Time) supplied by TaKaRa. The amplification products were quantified using SYBR Green (Vazyme) ...
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bioRxiv - Immunology 2023Quote: ... reverse transcription and cDNA pre-amplification were performed using the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Clontech/Takara). cDNA was harvested and quantified with the Bioanalyzer DNA High-Sensitivity kit (Agilent Technologies) ...
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bioRxiv - Physiology 2023Quote: ... Up to 30 ng of post-Immunoprecipitation dsDNA as measured by Invitrogen™ Qubit™ highsensitivity spectrofluorometric measurement was made into Illumina-compatible NGS libraries using the ThruPLEX® DNA-Seq kit from Takara Bio Inc ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... cDNA was synthesized from 100 µg of total RNA by Prime ScriptTM RT reagent kit with gDNA Eraser (Takara, Tokyo, Japan). Each cDNA sample was amplified for the gene of interest and β-actin in a 15 µL reaction volume TB GreenTM Premix Ex Taq™ II (Takara ...
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bioRxiv - Evolutionary Biology 2023Quote: Complete cDNA sequences of taste receptors were obtained using a SMARTer 5’/3’ kit for RACE PCR to obtain 5’ sequence information missing from reference transcriptome (Takara, #634858). Briefly ...
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bioRxiv - Immunology 2023Quote: ... This RNA was used as input to the Mir-X™ miRNA qRT-PCR TB Green® Kit (Takara cat# 638316) to generate miRNA cDNA ...
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bioRxiv - Immunology 2023Quote: ... The expression vectors for the S variants with multiple amino acid changes or deletions were generated using the In-Fusion HD cloning kit (Takara Bio). The pcDNA3.1-hACE2 used to express human ACE2 (hACE2 ...
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bioRxiv - Synthetic Biology 2024Quote: ... 23S rRNA mutations were introduced to the pLK35 plasmid using the corresponding primer set (Supplementary Table 5) and the In-Fusion Cloning kit (Takara Bio). All sequences were confirmed with full plasmid nanopore sequencing (Plasmidsaurus and Elim Bio).
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bioRxiv - Immunology 2024Quote: ... A total of 1 μg of total RNA was reverse-transcribed with random primers using the PrimeScript™ 1st strand cDNA Synthesis Kit (TaKaRa) according to the manufacturer’s protocols ...
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bioRxiv - Microbiology 2023Quote: ... the amplified fragment was incorporated into the Bam HI site of pHSG396 using the In-Fusion HD cloning kit (TAKARA, Japan), resulting in the generation of pHSG396-ptrA.
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bioRxiv - Cell Biology 2024Quote: ... GS linker human IgG1 FC region from pcDNA3-sACE2v2-Fc (IgG1) and PGK promoter puromycin resistance region cloned into lenti CAG-FLAG-dCas9-VPR using infusion cloning kit (Takara, 638947). After packaging the lentivirus ...
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bioRxiv - Developmental Biology 2024Quote: ... The fragment was then inserted in the pCAGEN-IRES-tomato with the In-Fusion® HD Cloning Kit (639649, Takara bio) according to manufacturer recommendations to generate pCAGEN-ATP1A3-WT-IRES-Tomato (named ATP1A3-WT) ...
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bioRxiv - Neuroscience 2024Quote: ... Titers were obtained on freeze-thawed aliquots using the qPCR-based TaKaRa lentivirus titration kit (#631235, Takara Bio, Kusatsu, Shiga, Japan). Minimum titers for all in vivo experiments was 1×1011 genome copies/mL ...
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bioRxiv - Immunology 2024Quote: ... The damaged lung areas of the infected mice and the corresponding lung areas of the uninfected control mice were excised to isolate total RNAs using TaKaRa MiniBEST Universal RNA Extraction Kit (TaKaRa, 9767), which were reverse transcribed into cDNAs using the RevertAid First Strand cDNA Synthesis Kit (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2024Quote: ... The extracted RNA was quantified by Nanodrop and 500 ng of it was converted into cDNA using a PrimeScript™ RT reagent Kit (Takara). Quantitative RT-PCR (qRT-PCR ...
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bioRxiv - Synthetic Biology 2024Quote: ... the rRNA-depleted and fragmented total RNA fraction was converted to sequencing libraries using the SMARTer® smRNA-Seq Kit for Illumina (Catalog number #635030) from Takara Bio USA ...
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bioRxiv - Cancer Biology 2024Quote: ... MCF-7 and ErbB2-overexpressing MCF-7 cells were routinely tested using the CycleavePCR Mycoplasma Detection Kit (Takara bio, CY-232). None of the cell lines were contaminated with mycoplasma.
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bioRxiv - Immunology 2024Quote: ... Samples were then processed for the preparation of libraries using the SMARTer Stranded Total RNA Sample Prep Kit (Takara Bio USA) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... and the amplicons were then ligated into a pUAST-attB vector digested by EcoRI/NotI using In-Fusion HD Cloning Kit (Takara, 639649). The landing site for each construct was {3xP3-RFP.attP}ZH-86Fb ...
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bioRxiv - Plant Biology 2024Quote: ... Total RNA was used to synthesize cDNA using the SMART-Seq v4 full-length transcriptome analysis kit from Takara (product # 634888) using protocol B specified in the manual on page 12 ...
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6-Phosphogluconolactonase is critical for the efficient functioning of the pentose phosphate pathwaybioRxiv - Biochemistry 2024Quote: ... coli BW25113 WT strain by PCR and inserted in pZA23 plasmid using the In-Fusion® HD Cloning Plus CE Kit (Takara).
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bioRxiv - Cancer Biology 2024Quote: ... and was reverse transcribed to make amplified cDNA by SMART seq v4 ultra-low input RNA kit (Takara Bio Inc, USA). After shearing of cDNA by Covaris ultrasound water bath sonicator and purification by Ampure beads (Beckman Coulter ...
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bioRxiv - Cell Biology 2024Quote: ... templates were constructed by cloning the 1.5 kb upstream and downstream homology arms into the pPD95.77 plasmid using the In-Fusion Advantage PCR cloning kit (Clontech, Cat. #639621). Fluorescence tags were inserted into the constructs with a flexible linker before the stop codons ...
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bioRxiv - Molecular Biology 2024Quote: ... Residual genomic and plasmidic DNA was removed using DNA-free kit (InvitrogenTM) and 500 ng of RNA were retro-transcribed with PrimeScript RT Master Mix (Takara Bio) following manufacturer instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... was PCR-amplified from SK-N-BE cells cDNA and cloned between the ICSs using In-fusion Cloning Kit (Takara Bio). 4xPepper array sequence (196 bp long ...
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bioRxiv - Molecular Biology 2024Quote: ... plasmid and cloned within exon 4 (between base 43-44) and exon 5 (between base 71-72) of the circ-HDGFRP3 sequence by In-Fusion Cloning Kit (Takara Bio) to obtain the final constructs p-circ-Ex4 and p-circ-Ex5.
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bioRxiv - Molecular Biology 2024Quote: ... Resulting amplicons were mixed with the digested backbone and joined in a single reaction by In-Fusion® HD Cloning Kit (Takara).
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bioRxiv - Cell Biology 2024Quote: ... of MMP9 or HSF2 were inserted into the linear vector upstream of the luciferase reporter gene using the In-Fusion HD cloning kit (Takara Bio) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... and cloning reactions were performed at 50 °C for 1 h using the In-Fusion® HD Cloning Kit (Takara Bio). The reaction mixtures were further transformed into TG1 electrocompetent cells (Lucigen) ...
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bioRxiv - Molecular Biology 2024Quote: ... Apoptotic cells were quantified via the terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay using the In situ Apoptosis Detection kit (Takara Bio). Visualization of apoptotic cells was performed using the Evos FL Cell Imaging System (Life Technologies).
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bioRxiv - Cancer Biology 2024Quote: ... The RNA was then reverse-transcribed into complementary DNA (cDNA) following the instructions of PrimeScript RT reagent kit (Takara, Beijing, China). qRT-PCR was performed using the AceQ Universal SYBR qPCR Master Mix (Vazyme) ...
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bioRxiv - Biochemistry 2024Quote: ... We introduced a T44C mutation and 108C mutation into the GB1-loop by site-directed mutagenesis using a PrimeSTAR mutagenesis basal kit (Takara Bio).
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bioRxiv - Pathology 2024Quote: Expression for IFN-related genes was conducted by reverse transcription using the PrimeScript RT Reagent Kit (Perfect RealTime, Takara Bio Inc.). qPCR reaction was performed in duplicate using Takyon ROX SYBR MasterMix blue dTTP (Eurogentec ...
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bioRxiv - Immunology 2024Quote: ... Library preparation of RNA samples was performed using the SMARTer Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian (Takara 634485). Two independent pooled libraries (samples from mice with spontaneous disease and samples from mice with pristane-induced disease ...
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bioRxiv - Microbiology 2024Quote: ... 500 ng supercoiled plasmid pBR322 obtained from the FastPure Plasmid Mini Kit (Vazyme, DC201-01) or relaxed plasmid pBR322 (derived from supercoiled pBR322 digested by Topoisomerase I, Takara, 2240A), along with 6 μl 5× buffer (170 mM Tris-HCl ...
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bioRxiv - Immunology 2024Quote: ... 2000 cell equivalent of RNA was used as input to SMART-seq v4 Ultra Low Input cDNA Synthesis kit (Takara, 634888) and 200 pg of cDNA was used to generate sequencing libraries with the NexteraXT kit (Illumina ...
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bioRxiv - Immunology 2024Quote: ... and then ten nanograms of total RNA was used as input for cDNA synthesis using the Clontech SMART-Seq v4 Ultra Low Input RNA kit (Takara Bio) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... Then the RNA-seq library was prepared using SMART-Seq® v4 Ultra® Low Input RNA Kit (TaKaRa Bio, USA). This was performed in Shpokayte et ...
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bioRxiv - Genomics 2024Quote: ... 152 ∗106 GPRTG cells were seeded into a 2-STACK culture chamber (Cornig) and transfected 24 h later with 238 µg transfer plasmid using the CalPhos Mammalian Transfection Kit (Takara Bio) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2020Quote: ... After 1 μg of total RNA from leaves was reverse-transcribed using a High Fidelity PrimeScript™ RT-PCR Kit (Takara, Japan), and Real-time qRT-PCR was performed using a Thermal Cycler Dice® Real Time System III T950 (Takara ...
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bioRxiv - Plant Biology 2020Quote: One µg of total RNA was used to generate cDNA with the SMARTer PCR cDNA Synthesis kit (Clontech, Mountain View, CA, USA). Fifty µl of cDNA were amplified by 13 PCR cycles with the Kapa HiFi PCR kit (Kapa Biosystems ...
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bioRxiv - Plant Biology 2019Quote: ... 1 μg DNA-free total RNA was used the template for synthesizing the first strand cDNA (PrimeScript II 1st Strand cDNA Synthesis Kit; Takara, Dalian, China). The primers of EcaICE1 and EcaHOS1 (listed as table 1 ...
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bioRxiv - Neuroscience 2021Quote: ... Library generation was performed using the SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian (cat no. 634412, Takara Bio Inc), then submitted to the MIT BioMicro Center for quality control (fragment analysis and qPCR) ...
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bioRxiv - Cancer Biology 2021Quote: ... and the oligonucleotides were inserted into C terminal of ARL4C-EGFP or ARL4C-FLAG-HA using In-Fusion HD Cloning Kit (Takara Bio Inc.).
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bioRxiv - Cell Biology 2020Quote: ... Point mutants were generated by site-directed mutagenesis using the In-Fusion HD cloning kit (TakaraBio/Clontech, Saint-Germain-en-Laye, France). Sequence correctness was verified by sequencing (LGC Genomics ...
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bioRxiv - Immunology 2021Quote: ... cDNA was synthesized and amplified directly from intact cells using SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Takara Bio USA) according to the manufacturer protocol ...
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bioRxiv - Microbiology 2020Quote: ... The above four fragments plus the fragment of the gentamycin resistance cassette flanked by loxp sites were connected and inserted into the linearized plasmid pUC19 using the In-Fusion Cloning Kit (TaKaRa, Daliang, China), generating the plasmid pUC-GSP ...
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bioRxiv - Microbiology 2020Quote: ... The PCR product was combined with fragment 2 and the gentamycin resistance cassette flanked by loxp sites and then inserted into the linearized plasmid pUC19 by an In-Fusion Cloning Kit (TaKaRa, Daliang, China) to generate the plasmid pUC19-Gent ...
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bioRxiv - Molecular Biology 2020Quote: ... An amount of 400 ng of the extracted RNA was subjected to reverse transcription by using a PrimeScript RT Reagent Kit (Takara, Shiga, Japan). With complementary DNA (cDNA ...
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bioRxiv - Microbiology 2020Quote: ... 250 ng of RNA isolated from each condition was converted into cDNA and processed through the SMARTer® 5’/3’ RACE Kit (Takara Bio) for 5’-RACE following manufacturer protocols ...