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Citations for Takara Bio :
4251 - 4300 of 5541 citations for Galactose Colorimetric Detection Kit 2 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: ... the gel-purified RNA was reverse transcribed into cDNA using a PrimeScript RT Reagent Kit with random primers (TAKARA, RR037B), followed by PCR with primers that can amplify transcripts across the splice junction ...
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bioRxiv - Cell Biology 2022Quote: ... and inserted between SpeI and NotI restriction sites (pFastBac1-hCAP-D3-[3C]-StrepII) by using restriction enzymes and a DNA ligation kit (TaKaRa). To create the hCAP-H2 construct with an N-terminal StrepII-tag (pFastBac-StrepII-[3C]-hCAP-H2) ...
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bioRxiv - Developmental Biology 2022Quote: ... Single-cell cDNAs were prepared on Fluidigm C1 system by using SMARTer v4 Ultra Input Low RNA kit for the Fluidigm C1 System (Clontech). Only single-cell cDNAs confirmed by imaging data were performed quality check (QC ...
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bioRxiv - Developmental Biology 2022Quote: ... the maximuM amount (10.5 µl) was used for library preparation using the SMART-Seq HT kit (Takara, Cat. No. 634437) followed by Nextera XT DNA Library Preparation Kit (Illumina ...
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bioRxiv - Cell Biology 2022Quote: ... full length double-stranded cDNA was prepared from 1 ng of total RNA according to the manufacturer’s instructions for the SMART-Seq v4 Ultra Low Input RNA Kit® (Clontech). Quantity and quality of the amplified full length double-stranded cDNA were assessed using both Qubit (Invitrogen ...
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bioRxiv - Neuroscience 2021Quote: ... RNA-Seq libraries were generated with the SMARTer Stranded Total RNA-Seq Kit - Pico Input Mammalian (v1 or v2; Takara) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2021Quote: ... DNAs of TOMM20-N-10-CLIPf and SNAPf-Clathrin-15 were directly PCR amplified from mammalian expression constructs and subcloned into lentiviral pHR-SFFV vector (BamHI/NotI) using In-Fusion HD Cloning kit (Clontech).
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bioRxiv - Neuroscience 2020Quote: ... Total RNA was extracted from the isolated tissue and was subjected to reverse transcription (RT) with the use of a NucleoSpin RNA extraction kit (U955C, Takara) and PrimeScript RT reagent kit (RR037 ...
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bioRxiv - Neuroscience 2020Quote: ... oligo(dT)-enriched RNA underwent 14 cycles of amplification using the SMARTer Ultra Low RNA Kit for Illumina Sequencing (Clontech). After cDNA fragmentation ...
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bioRxiv - Neuroscience 2020Quote: ... cDNA libraries were produced using the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Takara 634894, Lot 1709695A) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: 500 ng of the extracted total RNA were subjected to the first-strand cDNA synthesis with the primescript RT reagent kit (Takara). Quantitative real-time PCR was performed with Thunderbird SYBR qPCR mix (Toyobo ...
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bioRxiv - Plant Biology 2021Quote: The Pro197Ser-mutated ALS genes and the native form of MvALS1 gene were inserted into the pCAMBIA1390 vector under the cauliflower mosaic virus 35S promoter using the In-Fusion DH Cloning Kit (TaKaRa), as described previously (Iwakami et al. ...
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bioRxiv - Plant Biology 2021Quote: ... after-ripened Col and ref6c seeds collected at different imbibition time points were subjected for total RNA extraction using Minibest plant RNA extraction kit (Takara). Three independent biological replicates were performed ...
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bioRxiv - Plant Biology 2021Quote: ... The amplicons from SlACS2-prom1k-pPLV-F/R were cloned into pPLV451 using an In-Fusion HD Cloning kit (Clontech), to construct pACS2-GFPx3 and pACS2mut-GFPx3 ...
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bioRxiv - Microbiology 2020Quote: ... gene was then PCR amplified and purified then cloned into plasmid pLC29144 using In-Fusion HD Cloning plus kit (Takara). All PCR products and linearized vector were purified (Macherey and Nagel ...
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bioRxiv - Microbiology 2020Quote: ... overhangs upstream and downstream of the gene of interest were amplified (primers sequences available in Table S1) and purified then cloned into linearized pCM433 using In-Fusion HD Cloning plus kit (Takara), primer design was done using primer design tool (Takara) ...
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bioRxiv - Developmental Biology 2021Quote: ... Libraries were prepared from 50 ng of total RNA/sample as input using Ribogone and a SMARTer Universal Low Input RNA Kit (Clontech). For inDrops ...
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bioRxiv - Molecular Biology 2020Quote: Primary RT-PCR and secondary PCR was performed using the Primescript III 1-step RT-PCR kit and EmeraldAmp GT PCR Mix (Takara; detailed protocol provided in Supplementary File 1) ...
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bioRxiv - Genomics 2019Quote: ... The IsoSeq libraries were created starting from 1µg of total RNA per sample and full-length cDNA was then generated using the SMARTer PCR cDNA synthesis kit (Clontech) following PacBio recommendations set out in the IsoSeq method (pacb.com/wp-content/uploads/Procedure-Checklist-Iso-Seq-Template-Preparation-for-Sequel-Systems.pdf) ...
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bioRxiv - Molecular Biology 2019Quote: ... cerevisiae CLN2 was appended to the C-terminal codon of the HIS3 gene using the In-Fusion Cloning Kit (Clontech) as previously described (2) ...
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bioRxiv - Genomics 2021Quote: ... These nanowells were selected for subsequent targeted deposition of 50□nl/nanowell RT-PCR reaction mix from the SMARTer ICELL8 3’ DE Reagent Kit (Takara) using the MSND ...
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bioRxiv - Neuroscience 2021Quote: ... Ten ng of total RNA were amplified and converted into cDNA using a SMART-Seq v4 Ultra Low Input RNA Kit (Clontech). Next ...
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bioRxiv - Neuroscience 2020Quote: The cDNA libraries for used for sequencing of 4 total RNA samples were synthesized using a SMART-Seq Ultra Low Input RNA kit (Takara) in the OHSU Massively Parallel Sequencing Shared Resource Core Facility ...
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bioRxiv - Microbiology 2021Quote: ... PCR amplifications were conducted in 50-μL total volumes containing 1 μL of template DNA using an ExTaq kit (Takara). The cycling protocol was as follows ...
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bioRxiv - Plant Biology 2021Quote: Tagmented DNA was amplified directly from the beads via 6 cycles of PCR amplification using the PrimeSTAR GXL Polymerase kit (Takara) and dual indexed adapters (Illumina ...
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bioRxiv - Neuroscience 2021Quote: RNA from 30,000 cultured adult NSCs or whole DGs were isolated with the Clontech Nucleospin RNA XS Plus isolation kit (Takara 740990.10) per manufacturer protocol ...
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bioRxiv - Plant Biology 2021Quote: ... which was used to synthesize first-strand cDNA using the SuperScript II first-strand cDNA synthesis Kit™ (Takara, Japan). The Arabidopsis Actin2 gene was used as the internal reference gene.
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bioRxiv - Microbiology 2020Quote: The DNA samples were prepared for Illumina sequencing using the SMARTer® ThruPLEX® DNA-Seq kit (Takara Bio, USA) according to manufacturer protocol ...
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bioRxiv - Immunology 2020Quote: ... Libraries were constructed from total RNA with the ‘SMARTer Stranded Total RNA-Seq Kit v2 – Pico Input Mammalian’ (Takara/Clontech) according to manufacturer’s recommendations ...
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bioRxiv - Immunology 2020Quote: ... Libraries were constructed from total RNA with the ‘SMARTer Stranded Total RNA-Seq Kit v2 – Pico Input Mammalian’ (Takara/Clontech) according to manufacturer’s recommendations ...
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bioRxiv - Neuroscience 2020Quote: ... And then the DNA fragment encoding 4R1N tau were inserted into the linearized pHR-mCh-Cry2Olig backbone using In-Fusion Cloning Kit (Takara). The resulting constructs were fully sequenced to confirm the absence of unwanted substitutions.
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Physiological activation of the nephron central command drives endogenous kidney tissue regenerationbioRxiv - Physiology 2021Quote: ... Libraries were simultaneously prepared using Takara’s SMARTer Stranded Total-RNA Pico v2 library preparation kit following manufacturer’s protocol (Takara cat#634412). Prepared libraries were sequenced on Illumina Nextseq500 at 2×75cycles.
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bioRxiv - Microbiology 2021Quote: ... 72 °C 20 seconds for 35 cycles followed by subcloning into the eukaryotic expression vector pCAGGs using the In-Fusion HD cloning kit (Takara). All plasmids were midiprepped using the Nucleobond Xtra midi kit (Macherey-Nagel) ...
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Q-SNARE Syntaxin 7 confers actin-dependent rapidly replenishing synaptic vesicles upon high activitybioRxiv - Neuroscience 2020Quote: ... they were cloned into a SmaI site located downstream of the TRE sequence in pLenti6PW–TRE using an In–Fusion Cloning Kit (Clontech)(Egashira et al ...
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bioRxiv - Biophysics 2021Quote: ... The resulting DNA fragments were gel-extracted and cloned individually into the pBADC3 plasmid using InFusion EcoDry cloning kit (TaKaRa). All genes were fused with a C-terminal StrepII-tag for affinity purification.
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bioRxiv - Neuroscience 2022Quote: ... Ribo-depleted sequencing libraries were prepared with the SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian (TaKaRa Biotech) following manufacturer instructions and sequenced with Genewiz/Azenta on an Illumina NovaSeq S4 machine using a 2 ξ 150 bp pair-end read configuration to a minimum depth of 40 million reads per sample ...
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bioRxiv - Molecular Biology 2022Quote: Rps29 5’UTR and coding region cDNAs were synthesised using SMART™ RACE cDNA Amplification Kit (Clontech Laboratories Inc. USA). PCR was performed using gene-specific primers (40S 5’ RACE R ...
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bioRxiv - Developmental Biology 2022Quote: ... The picked endogenous IHCs and iOHCs were immediately subjected to reverse-transcription and cDNA amplification using a Smart-Seq HT kit (Cat# 634437, Takara). The post-amplified cDNA (1 ng ...
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bioRxiv - Genetics 2022Quote: ... The sequence of amplified MYSM1 cDNA was verified by Sanger sequencing before subcloning into the pLVX overexpression vector using the In-Fusion cloning kit (Takara). Q5 site directed mutagenesis kit was used to make H656R mutant.
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bioRxiv - Developmental Biology 2022Quote: ... Immunoprecipitated DNA and input control (pooled DNA that did not go through the IP step) were finally processed into sequencing libraries using PrepX DNA Library Kit (400075, Takara) and sequenced using the Illumina platform (NextSeq 500 ...
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bioRxiv - Microbiology 2022Quote: ... These two amplified fragments were inserted into the pBh-EPS vector [46] at the PstI site using the In-FusionHD Cloning Kit (Takara). The resultant plasmid and Bsu36I-digested BmNPV-abb genome DNA [47] were cotransfected into BmN-4 cells using FuGeneHD (Promega) ...
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bioRxiv - Neuroscience 2022Quote: The genomic location of the insertion in the enhancer-trap line Et(gata2:EGFP)bi105 was mapped using a linker-mediated approach with the Universal Genome Walker™ 2.0 Kit (Clontech). A detailed user manual is available online ...
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bioRxiv - Neuroscience 2022Quote: Total RNA from mouse cochleae was extracted using an RNA Extraction Kit according to the manufacturer’s instructions (#9767, TaKaRa, Japan). The extracted RNA concentration was measured using a Nanodrop 2000 spectrophotometer (#ND-LITE ...
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bioRxiv - Developmental Biology 2022Quote: ... was used to purify RNA and 500 ng RNA was used for reverse transcription with a Prime Script RT Reagent Kit (RR037A; Takara). Quantitative PCR was performed using a TB Green Premix DimerEraser Kit (RR091A ...
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bioRxiv - Genetics 2022Quote: ... Reverse transcription was performed using the oligo(dT) primer and avian myeloblastosis virus reverse transcriptase contained in the TaKaRa RNA PCR kit (TaKaRa). RT-qPCR was performed using the Kapa SYBR FAST qPCR kit (Kapa Biosystems ...
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bioRxiv - Cell Biology 2022Quote: ... reverse transcription and cDNA preamplification was performed using the SMARTer Ultra Low RNA Kit for the Fluidigm C1 System (Clontech) according to Fluidigm’s manual for mRNA sequencing on the C1 system ...
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bioRxiv - Neuroscience 2022Quote: Total RNA was prepared from cortical samples from control and cKO animals (3 animals/group) by using RNAiso-plus kit (Takara) and according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... with primers containing 15 bp tails homologous to cloning sites within the pGL4.10[luc2] vector which is necessary for cloning using the In-Fusion® HD cloning kit (Takara). Primer sequences are indicated in Table S1 ...
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bioRxiv - Microbiology 2022Quote: ... A total of 500 ng RNA was reverse transcribed into complementary DNA (cDNA) using a PrimeScript II 1st Strand cDNA Synthesis kit (TaKaRa) or a QuantiNova Reverse Transcript Kit (Qiagen ...
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bioRxiv - Molecular Biology 2022Quote: ... the GST gene from the pEGKG plasmid (29) was amplified by PCR and inserted immediately upstream of GFPS65T using the In-Fusion HD cloning kit (Takara).