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Citations for Takara Bio :
4201 - 4250 of 5541 citations for Galactose Colorimetric Detection Kit 2 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... Total RNAs of 0.5 to 1 μg were used as templates for the reverse transcription using PrimeScriptTM RT Reagent Kit with gDNA Eraser (Takara). Quantitative PCR (qPCR ...
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PPARγ is a tumor suppressor in basal bladder tumors offering new potential therapeutic opportunitiesbioRxiv - Cancer Biology 2019Quote: ... The GAL4 DNA-binding domain cloning vector pM and the activation-domain cloning vector pVP16 are part of the Mammalian Matchmaker Two-Hybrid Assay kit (BD Biosciences Clontech). The construct pM-MED1 (510-787 ...
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bioRxiv - Cell Biology 2020Quote: ... The plasmid pJYB240 was generated from pJYB212 by exchanging the mEos2 open reading frame by a PCR-amplified mTurquoise2 DNA (Goedhart et al. 2012) using the InFusion kit (Clontech). The parBF-mTurquoise2 gene from pJYB240 was introduced into pJYB243 ...
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bioRxiv - Plant Biology 2021Quote: ... and were used for cDNA synthesis and library preparation using SMARTer universal low input RNA kit (Clontech, Mountain View, CA). Library fragment size distribution was checked on the Agilent 2100 Tapestation System with the High Sensitivity D1000 Kit (Agilent Technologies) ...
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bioRxiv - Biochemistry 2021Quote: ... DNA was gel purified and inserted into pCDNA 3.1 vectors (CMV promoter) by making use of In-Fusion HD Cloning Kits (Takara Bio) as described in previous section ...
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bioRxiv - Biochemistry 2021Quote: ... DNA was gel purified and inserted into pCDNA 3.1 vectors (CMV promoter) by making use of In-Fusion HD Cloning Kits (Takara Bio). EcoR1 and HindIII digested vector was incubated with overlapping PCR fragments (of various different recombinant DNAs ...
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bioRxiv - Plant Biology 2020Quote: ... and reverse-transcribed with T7-oligo(dT)24 as a primer using the PrimeScript II 1st strand cDNA Synthesis kit (TaKaRa). Then the poly(A ...
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bioRxiv - Plant Biology 2020Quote: ... mRNA and miRNA from three independent biological samples were reverse transcribed into cDNA using the PrimeScript II 1st Strand cDNA Synthesis Kit (TaKaRa) and the miRcute Plus miRNA First-Stand cDNA Synthesis Kit (Tiangen) ...
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bioRxiv - Microbiology 2020Quote: ... The viral load was measured by RT-qPCR using One-Step SYBR® Primescript™ RT-PCR kit II (Takara). CT values of serum samples were used to calculate serum viral titre according to regression equation built by RNA extracted from 10 µL of 102-106 pfu/mL of ZIKV (PRVABC59 or MP1751) ...
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bioRxiv - Plant Biology 2020Quote: For β-galactosidase assay constructs were processed according to the instructions of the β-galactosidase assay kit provided by Clontech. For each construct ...
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bioRxiv - Microbiology 2020Quote: ... Double stranded cDNA was prepared from 1 μg of each mRNA sample using the PrimeScript Double Strand cDNA Synthesis Kit (Takara) and a cDNA library was established with Ligation Sequencing Kit (SQK-LSK109 ...
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bioRxiv - Microbiology 2020Quote: ... Japan) and then mRNA was purified from total RNA by Oligotex −dT30 Super mRNA Purification Kit (Takara Bio, Kusatsu, Japan). Double stranded cDNA was prepared from 1 μg of each mRNA sample using the PrimeScript Double Strand cDNA Synthesis Kit (Takara ...
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bioRxiv - Neuroscience 2020Quote: ... We constructed the T2A-QF2-9xQUAS-GCaMP6f-3XP3-dsRed donor plasmid (Fig. 2a) using the InFusion HD Kit (Clontech, 638910). To preserve the orco coding sequence ...
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bioRxiv - Microbiology 2021Quote: Viral RNA was extracted from 200 μL of pseudovirus using the MiniBEST Viral RNA/DNA Extraction Kit Ver.5.0 (TaKaRa, Japan), and the extracted mRNA was used as a template for reverse transcription using M-MLV reverse transcriptase (Promega ...
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bioRxiv - Neuroscience 2020Quote: ... The virus titer of purified AAV stocks was determined by AAVpro® Titration kit (for Real Time PCR) ver2 (TaKaRa).
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bioRxiv - Neuroscience 2020Quote: Four samples with 400 zfPGCs each were collected for each group (1 and 10 DIV) and RNA was extracted using the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (TAKARA) following manufactures instructions ...
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bioRxiv - Neuroscience 2020Quote: RNA-seq was carried out at New York University using Illumina HiSeq 4000 Paired-End 150 Cycle Lane from purified RNA (PicoPurePicoPure® RNA Isolation Kit KIT0204 Arcturus (ThermoScientific) and SMARTer Stranded Total RNA-Seq Kit - Pico Input Mammalian (250 pg–10 ng RNA) (635005, Clontech). The low-quality base (quality score lower than 20) ...
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bioRxiv - Neuroscience 2020Quote: ... which was performed according to the manufacturer’s guidelines using the iCell8 Chip and Reagent Kit using in-chip RT-PCR amplification chemistry (Wafergen, Takara Bio) and Nextera XT DNA Library Preparation Kit and Nextera XT Index Kit (Illumina) ...
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bioRxiv - Physiology 2021Quote: ... The RNA (1 ug/sample) was reverse transcribed into cDNA and Q-PCR was performed using a SYBR Green PCR kit (Takara) in a Light Cycler (Eppendorf) ...
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bioRxiv - Systems Biology 2021Quote: ... cDNA was synthesized from 1 µg of total RNA using the PrimeScript RT reagent kit (Perfect Real Time) (cat. no. RR037A, TaKaRa) following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... It was subsequently cloned into BamHI site in the pLenti CMV-IRES-GFP by In-Fusion cloning kit (Clontech, 639649) to generate lentiviral packaging plasmids(pLentiCMV-Y437H-IRES-GFP) ...
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bioRxiv - Microbiology 2020Quote: ... The 1.5 kb PCR product was isolated and purified from agarose gel using the NucleoSpin Gel and PCR Clean-up kit (Takara). The product was subjected to Sanger dideoxy sequencing at GENEWIZ (San Diego ...
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bioRxiv - Microbiology 2019Quote: ... The resulting product was purified from a 1% agarose gel using the NucleoSpin Gel and PCR Clean-up Kit (Takara) and Sanger sequenced.
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bioRxiv - Microbiology 2020Quote: ... Total protein was extracted from 2,000 midguts using the lysis buffer supplied in the Capturem IP & Co-IP kit (Takara, 635721). The extracted proteins were divided into two equal parts and used for immunoprecipitation (IP) ...
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bioRxiv - Biochemistry 2021Quote: ... Lep system including the sequences of interest in the P2 region were subcloned into KpnI linearized pCAGGS in-house version using In-Fusion HD cloning Kit (Takara) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: Whole Genome Amplification (WGA) was performed on 300 single cells for each Time by means of SMARTer PicoPLEX Single Cell WGA kit (Takara) following manufacturer’s protocol as previously described24 ...
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bioRxiv - Cancer Biology 2020Quote: ... RNAseq libraries were generated using total RNA as input with the SMART-Seq V4 Ultra Low Input RNA kit (Clontech). The cDNA libraries were confirmed on the Bioanalyzer using the High Sensitivity DNA kit (Agilent) ...
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bioRxiv - Cancer Biology 2020Quote: Transcriptome sequencing on low input total RNA samples (up to 10 ng) was carried out using SMART-Seq v4 Ultra Low Input RNA Kit (Clontech), according to manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... Lentiviral vectors expressing KDELR2 or a control empty vector were titered using the Lenti-X p24 rapid titer kit (Takara). 8 × 105 SH-SY5Y in 2 mL growth media were plated in a 6-well plate ...
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bioRxiv - Cell Biology 2021Quote: ... RT-PCR was performed using the One Step TB Green PrimeScript PLUS RT-PCR Kit (Perfect Real Time; Takara, Japan) and Thermal Cycler Dice® Real Time System Lite (TP700 ...
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bioRxiv - Cell Biology 2020Quote: ... The purified fragment was cloned into BamHI-SalI site of pLenti-PGK-GFP(34) with In-Fusion cloning HD kit (TAKARA). HEK293T cells were transfected with the pLenti-PGK-Tacc3 or pLKO.1 TRC (control ...
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bioRxiv - Biophysics 2020Quote: Target DNA containing the 5′-TTTA-3′ PAM was ordered from IDT and cloned into a pET28-MHL vector using the In-Fusion Cloning Kit (ClonTech). Plasmids were linearized before usage ...
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bioRxiv - Cell Biology 2020Quote: ... 1 μg of total RNA from each condition was converted into cDNA using PrimeScript RT reagent with gDNA Eraser kit (Takara Clontech) and MJ Research PTC-220 Dyad PCR System (Conquer Scientific) ...
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bioRxiv - Cell Biology 2021Quote: ... Libraries for RNA-sequencing were prepared using the SMARTer Stranded Total RNA-Seq Kit v2 Pico Input Mammalian library preparation protocol (Takara Bio USA ...
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bioRxiv - Molecular Biology 2022Quote: ... RNA was isolated using the TRIzol method and the expression of miRNAs analyzed by qRT-PCR with the Mir-X miRNA qRT-PCR TB Green kit (Takara Bio Inc ...
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bioRxiv - Physiology 2022Quote: ... cDNA was generated and amplified using the Smart-SEQ HT Kit for low RNA input according to the manufacturer’s recommendations (Takara Bio), which was used for generating sequencing libraries with the Nextera XT kit ...
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bioRxiv - Cell Biology 2022Quote: ... and LSK+ or LSK- RNA-seq libraries were prepared using SMART-Seq v4 Ultra Low Input RNA Kit (Takara, 634888) and KAPA HyperPrep kit (KAPA ...
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bioRxiv - Biochemistry 2022Quote: ... cDNA synthesis was carried out using the Primescript™ RT reagent kit with gDNA eraser (perfect real time) (TaKaRa, JP) following the manufacturer’s instructions.
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bioRxiv - Developmental Biology 2022Quote: Trophectoderm biopsies containing 5-10 cells from blastocyst-stage embryos (n=24) were processed for RNA-seq using a commercial kit (Takara Bio ...
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bioRxiv - Molecular Biology 2022Quote: ... First-strand cDNA was synthesized from the total RNA using a PrimeScript™ RT Reagent Kit with gDNA Eraser (Takara) following the manufacturer’s protocols ...
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bioRxiv - Immunology 2022Quote: ... – PU6-pAzpa was co-transfected with plasmids expressing full-lengths mutant 2B4 TCR and CD3 subunits using Xfect transfection kit (TaKaRa). For incorporating pAzpa into TCR sites ...
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bioRxiv - Plant Biology 2022Quote: ... PCR fragments representing either the OsSOG1 promoter+GUS gene or OsSGL promoter+GUS gene were inserted into the AscI/AvrII site using an In-Fusion HD cloning kit (Takara) between attL1 and T35S in pE(L1-L2)hpt ...
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bioRxiv - Microbiology 2022Quote: ... Then RNA was reverse-transcribed and amplified using One Step PrimeScriptTM III RT-qPCR Mix kit (Takara Bio, Kyoto, Japan). Primers and probes ...
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bioRxiv - Immunology 2022Quote: ... First-strand cDNA was generated from 1 μg of RNA for each sample using the SMARTer RACE 5’/3’ Kit (Takara Bio ...
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bioRxiv - Cell Biology 2022Quote: ... reverse transcription and cDNA synthesis were performed using the SMART-Seq v4 Ultra Low RNA Kit for the Fluidigm C1 System (Takara) reagents ...
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bioRxiv - Cell Biology 2022Quote: ... For each specimen 500 ng of total RNA was reversed transcribed into cDNA using PrimeScript RT reagent kit (TaKaRa RR037a) and oligo dT primers according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2022Quote: ... PtoATPE and PtoPSBB) were co-transformed into the Y1HGold yeast strain using the Matchmaker One-Hybrid Library Construction and Screening Kit (Clontech), respectively ...
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bioRxiv - Microbiology 2022Quote: ... One μg of total RNA was used as input for SMARTer Stranded Total RNA Sample Prep Kit-HI Mammalian (Clontech). Sequencing was performed on the NextSeq500 instrument (Illumina ...
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bioRxiv - Molecular Biology 2022Quote: ... Ribo-depleted sequencing libraries were prepared with the SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian (TaKaRa Biotech) following manufacturer instructions and sequenced with Genewiz/Azenta on an Illumina NovaSeq S4 machine using a 2 x 150 bp pair-end read configuration to a minimum depth of 30 million reads per sample ...
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bioRxiv - Biophysics 2022Quote: ... 5’ and 3’-RACE-Ready cDNA templates were synthesized using a SMARTer® RACE 5’/3’ Kit (Takara Bio, USA) and subsequently used to amplify 5’ and 3’ end sequences of P ...