Labshake search
Citations for Takara Bio :
351 - 400 of 893 citations for M CHERRY MRNA mCherry Fluorescent Protein coding mRNA since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2021Quote: ... the coding sequences of human Pcnt B and Pcnt S were subcloned into peGFP-N1 (Takara Clontech) using the NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs ...
-
bioRxiv - Cell Biology 2021Quote: ... the coding sequences of human Pcnt B and Pcnt S were subcloned into peGFP-N1 (Takara Clontech) using the NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs ...
-
bioRxiv - Biophysics 2019Quote: ... and the mCherry gene was obtained from the pmCherry-N1 plasmid (Takara Bio). Reaction products were transformed ...
-
bioRxiv - Immunology 2021Quote: ... Blots were stained with the mouse α-GFP or α-mCherry (Clontech, EU) antibodies.
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... was cloned into a bicistronic lentiviral expression vector pLVX-EF1α-IRES-mCherry (Clontech) creating pLVX-EF1α-IRES-mCherry-NLuc ...
-
bioRxiv - Cell Biology 2019Quote: ... cDNAs of mCherry and puromycin resistance were amplified from pmCherry-N1 (632523, Clontech) and pICE vector (Addgene #46960)33 ...
-
bioRxiv - Microbiology 2021Quote: ... The membranes were then incubated with either primary monoclonal antibodies anti-mCherry (Clontech #632543 ...
-
bioRxiv - Biophysics 2020Quote: ... The mCherry gene (AgeI / NheI) was obtained by PCR on pmCherry-N1 (Clontech). A four-fragment-ligation was then done to obtain the final construct (HindIII-HA-Nlg1-AgeI-mCherry-NheI-Nlg1Cter-NotI) ...
-
bioRxiv - Cell Biology 2022Quote: ... Cytosolic GFP (pEGFP-N1) and mCherry (pmCherry-N1) vectors were purchased from Clontech. H2B-mCherry was a gift from Robert Benezra (Addgene plasmid #20972 ...
-
bioRxiv - Cell Biology 2023Quote: ... delCMV- mCherry was generated by ligating the larger fragment from pmCherry-N1 (Clontech) with the smaller fragment from delCMV-mCherry-actin (Schulze et al. ...
-
bioRxiv - Neuroscience 2023Quote: ... was similarly constructed with the leptin receptor promoter controlling expression of mCherry (Clontech) without ChR2 ...
-
bioRxiv - Developmental Biology 2024Quote: Primary antibodies: Living Colors Polyclonal anti-mCherry/dsRed (rabbit, 1:500, Clontech 632496), anti-GFP (chicken ...
-
Mck1 defines a key S-phase checkpoint effector in response to various degrees of replication threatsbioRxiv - Molecular Biology 2019Quote: ... DNA was transferred to HyBond N+ in transfer buffer (0.4 M NaOH, 1 M NaCl) and UV cross-linked before hybridization with a random primed probe (Takara) overnight at 42°C and washed twice for 20 min with 0.5× SSC 0.1% SDS at 65°C.
-
bioRxiv - Cell Biology 2020Quote: ... fused with the SYFP2 coding region using recombinant PCR and cloned into pMSCV-Thy-IRES 1.1 vector (Clontech) using EcoRI/XhoI and ClaI restriction sites.
-
bioRxiv - Microbiology 2019Quote: The coding sequence of DsRed was isolated from the pDsRed-monomer vector (Clontech/TaKaRa Bio, Ohtsu, Shiga, Japan) by digestion with the appropriate restriction enzymes ...
-
bioRxiv - Microbiology 2019Quote: The coding sequence of DsRed was isolated from the pDsRed-monomer vector (Clontech/TaKaRa Bio, Ohtsu, Shiga, Japan) by digestion with the appropriate restriction enzymes ...
-
bioRxiv - Molecular Biology 2020Quote: ... we inserted the coding sequence between the SalI and BamHI restriction sites of the pAcGFP1-N1 plasmid (Takara). The total RNA was extracted from R ...
-
bioRxiv - Evolutionary Biology 2021Quote: The gene fragments in the coding region of lg in zebrafish and tilapia were amplified using PrimerSTAR (Takara) (Table S7 ...
-
bioRxiv - Genomics 2021Quote: The coding sequences for 23 tomato HSFs and 21 Arabidopsis HSFs were cloned into the pGADT7 vector (Clontech) as a prey (Supplementary table S8) ...
-
bioRxiv - Physiology 2022Quote: ... The insulin-like growth factor 1 (IGF1) coding sequence was PCR amplified (CloneAmp, Takara Bio; Cat. No. 639298) from cDNA generated from a rat gastrocnemius muscle ...
-
bioRxiv - Physiology 2022Quote: The coding sequence of PIK3CA was then cloned PIK3CA into the pcDNA3.4 vector through in-fusion cloning (Clontech) as an untagged protein or with an N-terminal polyhistidine tag ...
-
bioRxiv - Molecular Biology 2023Quote: A DNA fragment coding DDX43 or DDX6 was amplified by PCR and cloned into pCold-I vector (Takara) by In-fusion cloning kit (Takara).
-
bioRxiv - Cell Biology 2023Quote: ... Full-length human CLUH and mouse Cluh coding sequences cDNA were cloned in pcDNA3 and pmCherry-N1 (Clontech), respectively ...
-
bioRxiv - Developmental Biology 2024Quote: ... Human full-coding KATNA1 cDNA was isolated from a human fetal brain Marathon-ready cDNA collection (Clontech, Invitrogen) with the following forward and reverse primers ...
-
bioRxiv - Cell Biology 2020Quote: ... mCherry-labeled PACSIN2 was prepared by subcloning PACSIN2 cDNA into pmCherry-C1 vector (Clontech), in which the GFP in pEGFP-C1 was replaced with mCherry ...
-
bioRxiv - Cell Biology 2019Quote: ... β2AR-mCherry was made by transferring β2AR from β2AR-mCFP to pmCherry-N1 (Clontech) using NheI/XhoI ...
-
bioRxiv - Cell Biology 2019Quote: ... LAMP1-GFP was made by transferring LAMP1 from LAMP1-mCherry into pEGFP-N1 (Clontech) using EcoRI/BamHI ...
-
bioRxiv - Biochemistry 2022Quote: ... was added at the N-termini of EGFP and mCherry (Clontech, Mountain View, CA). For intramolecular FRET experiments ...
-
bioRxiv - Neuroscience 2022Quote: ... with the mCherry sequence by the In-Fusion HD Cloning Plus kit (Takara, 638910). For the transposon-based p53 reporter assay performed on KOLF2.1J ...
-
bioRxiv - Neuroscience 2021Quote: ... rabbit Living Colors anti-DsRed (used for mCherry, tdTomato; 1:1000; Takara Bio, 632496); goat anti-RFP (used for mCherry ...
-
bioRxiv - Molecular Biology 2023Quote: ... 14-3-3γ CDS was amplified by PCR and subcloned into pCMV-mCherry (Clontech). Deletion and point mutations of SMAUG1 were created using Q5 site-directed mutagenesis kit (NEB) ...
-
bioRxiv - Neuroscience 2023Quote: ... or control mCherry were subcloned using the InFusion HD cloning kit (Takara Bio USA). Furthermore ...
-
bioRxiv - Cell Biology 2024Quote: ... the annealed LifeAct was fused to N-terminal of mCherry-N1 vectors (Takara, #632523). Generation of pFN21A-HaloTag-actin was described previously21.
-
bioRxiv - Molecular Biology 2019Quote: ... and fluorescent double-labeled probes were synthesized by Takara (Japan). All of the DNA and RNA sequences are provided in Table S1 ...
-
bioRxiv - Molecular Biology 2019Quote: ... We inserted a green fluorescent C-terminal tag (EGFP; Clontech) analogous to a procedure used in a previous study [34] (for details ...
-
bioRxiv - Cell Biology 2020Quote: ... The emerin and LEM2 coding regions were amplified from HeLa cells derived cDNA and cloned into pEGFP-N3 (Clontech). pEGFP-N3-derived plasmids encoding for LAP2β (Ungricht et al. ...
-
bioRxiv - Molecular Biology 2021Quote: ... were used to amplify the full-length coding sequences CDS amplified with Tks Gflex™ DNA Polymerase (TaKaRa, Japan) according to manufacturer’s directions with an annealing temperature of 56.4°C ...
-
bioRxiv - Molecular Biology 2021Quote: ... Coding regions of the heavy-chain variable domains were amplified using LA Taq polymerase (TAKARA Bio Inc., Shiga, Japan) with two PAGE-purified primers (CALL001 ...
-
bioRxiv - Cell Biology 2019Quote: The rescued mEFs (expressing vimentin) were created by PCR amplification of the vimentin coding sequence using CloneAmp polymerase (Clontech) from pcDNA4- vimentin (provided by J ...
-
bioRxiv - Biochemistry 2020Quote: Mutations were introduced to plasmids coding for PALI1 and its truncations using Takara PrimeSTAR HS DNA Polymerase (Clontech #R045A), with primers indicated in Supplementary Table 2 ...
-
bioRxiv - Molecular Biology 2021Quote: ... Human eRF1 coding sequence was cloned into the XhoI and HindIII sites of the pλN-HA-C1 vector (Clontech). The pλN-HA-C1-eRF1 plasmid was then used to generate the pλN-HA-C1-eRF1AAQ (G183A G184A ...
-
bioRxiv - Plant Biology 2022Quote: ... The coding sequences of PWO2 and the fragment of PWO3 were cloned in the pGBKT7 and pGADT7 vectors (Clontech), passing through pDONR221 (Invitrogen ...
-
bioRxiv - Molecular Biology 2021Quote: Plasmid expressing mCherry-myc-TRIM1 was created by cloning myc-TRIM1 into pmCherry-C1 (Clontech). Plasmid expressing EBFP2-14-3-3 theta was created using HiFi Cloning (NEB ...
-
bioRxiv - Synthetic Biology 2021Quote: ... The vector for the mAID-mCherry expression was generated using the pEGFP-C1 backbone (Clontech), replacing the GFP gene with the mAID-mCherry cassette derived from pMK292 mAID-mCherry2-NeoR (Addgene #7283033) ...
-
bioRxiv - Neuroscience 2023Quote: ... followed by blocking solution containing primary antibodies against mCherry (mouse monoclonal 1:500, Takara Bio) and c-Fos (rabbit polyclonal 1:500 ...
-
bioRxiv - Cell Biology 2023Quote: ... mCherry and Venus ORFs were inserted into the MCSs of pBI-CMV1 (631630, Clontech, TakaraBio). WT ...
-
bioRxiv - Bioengineering 2023Quote: ... GFP BrightComp eBeads™ Compensation Bead Kit (ThermoScientific) and mCherry Flow Cytometer Calibration Beads (Takara). Flow cytometry of stained T lymphocytes was then performed using a BD LSRFortessa Cell Analyzer (BD Biosciences ...
-
bioRxiv - Cell Biology 2020Quote: ... M-MLV reverse transcriptase and RNase inhibitor (TaKaRa, D2639A). Q-PCR was performed in triplicate with Power SYBR® Green (Thermo Fisher Scientific ...
-
bioRxiv - Developmental Biology 2019Quote: ... and retrotranscription was performed with M-MLV retrotranscriptase (Clontech). Subsequently ...
-
bioRxiv - Plant Biology 2019Quote: For Yeast-two-Hybrid (Y2H) assays coding sequences were cloned into pDONR207 and recombined into the pGDAT7 and pGBKT7 (Clontech). Using the co-transformation techniques41 these constructs were transformed into the AH109 strain (Clontech).