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Citations for Takara Bio :
451 - 500 of 893 citations for M CHERRY MRNA mCherry Fluorescent Protein coding mRNA since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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ER exit sites in Drosophila display abundant ER-Golgi vesicles and pearled tubes but no megacarriersbioRxiv - Cell Biology 2021Quote: ... the coding sequence of each gene was amplified from whole larva cDNA using PrimeScript RT-PCR Kit (Takara, cat # RR014-A). The resulting products were recombined into pDONR221 and transferred to the pTWGA vector to finally produced the desired plasmids ...
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bioRxiv - Immunology 2022Quote: ... The pINDUCER20-EYFP-mCITED1 lentiviral construct was produced by amplifying the full-length murine CITED1 coding sequence from pCDNA3-Flag-mCITED1 and subcloning it into pEYFP-C1 (Takara/Clontech) in-frame with the EYFP coding sequence ...
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bioRxiv - Cell Biology 2023Quote: ... The coding sequence of EPHX2 was amplified by PCR from liver cDNA (Human Multiple Tissue cDNA panels; Takara Bio, Shiga, Japan) using primers (5’-GTCGACATGACGCTGCGCGCGGCCGTCTTCG-3’ ...
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bioRxiv - Cancer Biology 2023Quote: ... MYCN coding region was PCR amplified from HA-MYCN construct and cloned into the doxycycline inducible pLVX-pTetOne-puro vector (Takara Bio) using In-Fusion HD kit (Takara Bio ...
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bioRxiv - Microbiology 2023Quote: ... A parallel virus production was obtained by substitution of the ISG20 coding viral genome with pRetroX-Tet-On (Clontech, cat. 632104) that codes for the TetOn protein and allows for dox ...
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bioRxiv - Cancer Biology 2022Quote: ... the coding region of RAS cDNA was first PCR-amplified from the plasmids above by using CloneAmp HiFi PCR Premix (Takara #639298) and the primers listed in Supplementary Table 3 using manufacturer’s recommended PCR conditions ...
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bioRxiv - Immunology 2023Quote: ... ORF9c or ORF10 accessory proteins coding sequences (codon-optimized for mammalian expression) were cloned into pLVX-EF1α-IRES-Puro Cloning and Expression Lentivector (Clontech, Takara, #631253) to generate pseudotyped lentiviral particles encoding each accessory protein of SARS-CoV-2 (Wuhan-Hu-1 isolate ...
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bioRxiv - Cell Biology 2024Quote: ... the coding sequence of each gene was amplified from whole larva cDNA using PrimeScript RT-PCR Kit (Takara, cat # RR014-A). The amplified sequence was then purified through gel extraction (Magen HiPure Gel Pure DNA Mini kit ...
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bioRxiv - Immunology 2024Quote: ... cDNA was subsequently synthesized using Reverse Transcriptase M-MLV (TakaRa) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: Fluorescent constructs were introduced into cells using the pLVX lentiviral system (Clontech) and selected using antibiotic resistance to either puromycin or neomycin ...
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bioRxiv - Plant Biology 2022Quote: The coding region of each P450 gene was cloned into pCAMBIA1390 vector with the In-Fusion DH Cloning Kit (TaKaRa, Kusatsu, Japan) or SLiCE reaction (Motohashi ...
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DRT111/SFPS splicing factor controls ABA sensitivity in Arabidopsis seed development and germinationbioRxiv - Plant Biology 2020Quote: ... the coding sequence of DRT111 was cloned into the BamH1 and XhoI restriction sites of pGADT7 vector (Clontech, Mountain View, CA, USA) and the cDNA fragments of SF1 were cloned into the SmaI and SalI sites of pGBKT7 (Clontech ...
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bioRxiv - Plant Biology 2023Quote: ... The coding region of PIF4 was amplified and cloned into the yeast GAL4 activation domain (GAL4 AD) of pGADT7-Rec2 (Clontech, CA, USA) prey vector ...
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bioRxiv - Cancer Biology 2023Quote: The MDM4 coding sequence (NM_002393.5) was cloned into Lenti-X™ Tet-One™ Inducible Expression System (Takara Bio, cat. no. 631847). The resulting plasmid was then subject to whole-plasmid sequencing for verification ...
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bioRxiv - Plant Biology 2023Quote: For Yeast-two-Hybrid (Y2H) experiments coding DNA sequences were cloned into pDONR207 and recombined into pGDAT7® or pGBKT7® vectors (Clontech). Plasmids were transformed into the AH109 yeast strain by the lithium acetate (LiAc ...
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bioRxiv - Plant Biology 2023Quote: The full-length coding sequences (CDS) of NPR1 and TGA were fused to the bait vector pGBKT7 (Clontech, Palo Alto, CA, USA) to generate BD-NPR1 and BD-TGA ...
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bioRxiv - Cancer Biology 2022Quote: KPC cells were transduced with either an empty vector (EV) pLVX-IRES-mCherry (Clontech, Mountain View, CA) or the same backbone vector expressing the human K17 open reading frame cDNA ...
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bioRxiv - Neuroscience 2020Quote: ... The APP-mCherry plasmid was generated by introducing the APP751 sequence in the pmCherry-N1 vector (Clontech) at the XmaI/AgeI restriction site ...
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bioRxiv - Cell Biology 2020Quote: ... mCherry was introduced into the PIN2 construct by inverse PCR followed by an In-Fusion reaction (Clontech). The SNAP-tag with an N-terminal secretion signal peptide sequence (MKTNLFLFLIFSLLLSLSSAEF ...
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bioRxiv - Cell Biology 2020Quote: ... RRID:Addgene_17662) with an N-terminal mCherry fusion behind an EF1α promoter in a pLVX backbone (Takara Bio). The promoter plus gene fusion was then cloned into the pEGFP-BAF backbone by PCR and ligation ...
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bioRxiv - Molecular Biology 2022Quote: ... and digested with SacI and XbaI enzymes in M buffer (TaKaRa). The gene sequence included a SacI restriction site in the second intron and ...
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bioRxiv - Cancer Biology 2022Quote: ... Reverse transcription was performed using the M-MLV reverse system (Takara) to obtain cDNA ...
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bioRxiv - Synthetic Biology 2021Quote: ... The SYBR® fluorescent dye (TB Green® Premix Ex Taq™, Takara) was employed for reporting the amplification of cDNA in the Applied biosystem 7500 Fast Real-Time PCR system with specific primer pairs for DFR gene (Supplementary Table 2) ...
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bioRxiv - Cell Biology 2021Quote: Each fluorescent DNM2 construct utilized was cloned into the pEGFP-N1 vector (Clontech) as described in Tassin et al.18 to generate C-terminal EGFP fusion constructs.
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bioRxiv - Biophysics 2019Quote: ... The pCD86-EGFP had been created by inserting CD86 with a 21 amino acid linker coding region on its C-terminus immediately before EGFP in pEGFP-N1 (Clontech/Takara, Mountain View, CA). The CD86-21aa was amplified from pCD86-mEos2 (Mike Heilemann via Addgene ...
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bioRxiv - Systems Biology 2019Quote: ... PCR amplification allowed to obtain the coding sequence for human HSF1 that was cloned into peGFP N3 vector (Clontech Laboratories Mountain View, CA); the plasmid was then verified by sequencing (GATC Biotech ...
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bioRxiv - Physiology 2022Quote: ... The rat IGF1 coding sequence was then inserted into the linearized scAAV-CMV plasmid using In-Fusion cloning (Takara Bio; Cat. No. 639650). The resulting plasmids for scAAV-CMV-GFP and scAAV-CMV-IGF1 were packaged using AAV2/9 serotype by Vector Biolabs (Malvern ...
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bioRxiv - Plant Biology 2023Quote: ... The DNA segments coding MIR319A and MIR319B loci (with genomic DNA as the template) were amplified using PrimeSTAR HS DNA polymerase (TaKaRa Bio, Kusatsu, Japan) with miR319aF-miR319R or miR319bF-miR319R primer pairs and were sequenced according to Sanger method ...
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bioRxiv - Cancer Biology 2020Quote: ... The mCherry-NTF2 fragment was excised from pDL23 at AgeI/BclII and cloned into pTetOne (#634303 Clontech, Takara) at AgeI/BamHI to generate pTetOne mCherry-NTF2 (pDL65) ...
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bioRxiv - Cancer Biology 2020Quote: ... The mCherry-NTF2 fragment was excised from pDL23 at AgeI/BclII and cloned into pTetOne (#634303 Clontech, Takara) at AgeI/BamHI to generate pTetOne mCherry-NTF2 (pDL65) ...
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bioRxiv - Molecular Biology 2019Quote: ... Then the mCherry cassette was replaced by Puro or EGFP by In-Fusion® HD Cloning Kit (Clontech). To generate the enCRISPRi-LK sgRNA vector ...
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bioRxiv - Molecular Biology 2021Quote: ... a plasmid expressing mCherry under the CMV promoter (pmCherry) was cloned by removing EGFP from pEGFP-C1 (Clontech) with AgeI and BglII and assembling the vector with a synthesized mCherry gene fragment (IDT ...
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bioRxiv - Developmental Biology 2020Quote: ... 1×106 E14 ESCs were transfected with the two appropriate pSPCAs9(Guide)-2A-mCherry vectors using Xfect (Clontech). 48 hours after transfection cells were sorted for high levels of mCherry expression and plated onto 10 cm gelatinized dishes ...
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bioRxiv - Plant Biology 2022Quote: ... For Y2H assays, cDNAs of mCherry and ZmSIPs (ZmSIP1, ZmSIP2, ZmSIP3) were cloned into the pGADT7 vector (Clontech) for expression via the GAL4 AD under constitutive ADH1 promoter ...
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bioRxiv - Cancer Biology 2024Quote: ... we sub-cloned TVA-2A-mCherry-2A-oGlycoprotein into a lentiviral vector (‘pFU-‘) using In-Fusion cloning (Takara). TVA-2A-mCherry-2A-oGlycoprotein was amplified from p306 (Zurich virus core) ...
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bioRxiv - Cell Biology 2023Quote: ... EGFP and mCherry tagged cDNA constructs were cloned into pBMN and modified pLXIN (Clontech, Mountain View, CA, USA) retroviral expression vectors(Peden et al. ...
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bioRxiv - Bioengineering 2023Quote: ... a PGK promoter-driven mCherry expression cassette and the WPRE element were inserted into pMSCV (Clontech, Takara Bio) at the XhoI and ClaI sites to generate an empty vector (pMSCV-mCherry-WPRE) ...
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bioRxiv - Bioengineering 2023Quote: ... a PGK promoter-driven mCherry expression cassette and the WPRE element were inserted into pMSCV (Clontech, Takara Bio) at the XhoI and ClaI sites to generate an empty vector (pMSCV-mCherry-WPRE) ...
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bioRxiv - Physiology 2021Quote: Plasmid DNA encoding enhanced green fluorescent (EGFP)-actin (Takara Bio USA, Mountain View, CA) facilitated exogenous gene expression in rodent kidneys ...
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bioRxiv - Cell Biology 2020Quote: ... The cDNA was amplified using a fluorescent quantitative polymerase chain reaction (PCR) kit (TaKaRa) and an instrument (Applied Biosystems 7500 ...
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bioRxiv - Microbiology 2021Quote: ... cDNA was synthesized using M-MLV reverse transcriptase (TaKaRa Biotechnology, Dalian, China). In brief ...
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bioRxiv - Immunology 2022Quote: ... and first strand cDNA was synthesized with Reverse Transcriptase M-MLV (TaKaRa). Real-time PCR was performed using Hieff® qPCR SYBR Green Master Mix (No Rox ...
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bioRxiv - Developmental Biology 2020Quote: ... Reverse transcription was performed by using reverse transcriptase M-MLV (TaKaRa, Japan) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... was subcloned into the lentiviral expression vector pLVX-M-puro (Takara 632164). For CRISPR-KO experiments ...
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bioRxiv - Biophysics 2019Quote: ... The pCD86-EGFP had been created by inserting CD86 with a 21 amino acid linker coding region on its C-terminus immediately before EGFP in pEGFP-N1 (Clontech/Takara, Mountain View, CA). The CD86-21aa was amplified from pCD86-mEos2 (Mike Heilemann via Addgene ...
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bioRxiv - Neuroscience 2019Quote: ... A SARM1-GFP expression vector consisting of the entire coding region of murine SARM1 cloned (by PCR) into pEFGP-N1 (Clontech, C-terminal eGFP tag) was used for re-expression of SARM1 in Nmnat2gtE/gtE;Sarm1−/− neurons ...
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bioRxiv - Cell Biology 2019Quote: Human PDE4D5 wild type and PDE4D5-dN mutant were PCR amplified and cloned into pLVX-mCherry-N1 vector (Clontech) using Gibson assembly after vector digestion with XhoI and EcoRI ...
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bioRxiv - Microbiology 2019Quote: ... Sequences encoding primate PBMs were added to the resulting pDONR221-mCherry-human GBP1DC_BglII vector following BglII digestion using Ligation-Independent cloning (In-Fusion, Clontech) with annealed oligomers that also restored human GBP1 Q580-L581 and the human GBP1 CaaX box (Table S9) ...
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bioRxiv - Neuroscience 2023Quote: ... mCherry was introduced into the pcDNA™3.1-BspEI-LRP10 plasmid via PCR amplification from the pmCherryN1 plasmid (Clontech) via the addition of BspEI restriction sites in the primer sequences ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... Brain sections were transferred to positively charged glass histology slides (Fisherbrand) and immunostained with mCherry (rabbit-anti-DsRed; Takara Bio ...