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Citations for Takara Bio :
3801 - 3850 of 5321 citations for Human Lysine K Specific Demethylase 1A KDM1A ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: Full-length cDNA was synthesized with 1.0 μg RNA from Diphasiastrum complanatum using the SMARTer PCR cDNA Synthesis kit (Clontech). The first-strand cDNA was amplified with PrimeSTAR GL DNA polymerase (Clontech ...
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bioRxiv - Biochemistry 2023Quote: ... cDNA was prepared from 1 µg of total RNA using the PrimeScript RT Reagent Kit with gDNA Eraser (Takara). Real-time polymerase chain reaction (real-time PCR ...
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bioRxiv - Genomics 2023Quote: ... Genomic DNA of the cell pellets was extracted and purified using a Nucleospin Blood XL kit (Takara Bio, #740950.10). Guides were amplified and barcoded by PCR using NEB Next Ultra ii Q5 MM (M0544L ...
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bioRxiv - Immunology 2023Quote: ... Samples were rRNA depleted and prepared for sequencing using SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian (Takara). In brief ...
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TIPRL1 and its ATM-dependent phosphorylation promote radiotherapy resistance in head and neck cancerbioRxiv - Cancer Biology 2023Quote: ... Wild-type (WT) TIPRL1 cDNA was cloned into 3xFLAG pLenti6.4 using In-Fusion® HD Cloning Kit (Takara, #639650). Phosphomimic and non-phospho mutants (S265D and S265A ...
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bioRxiv - Neuroscience 2023Quote: ... and the target bands at 14,438 bp were cut and purified with NucleoSpin® Gel and PCR Clean-Up kit (740609.250, Takara). The target amplicons were re-concentrated to > 300 ng/ µL with GlycoBlue™ Coprecipitant (AM9515 ...
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bioRxiv - Neuroscience 2023Quote: ... Constructs containing point-mutated ARE-like sequences were prepared using the PrimeSTAR Mutagenesis Basal Kit (Takara Bio, Shiga, Japan). Because an ARE half-site can function alone to confer androgen inducibility (Pihlajamaa et al. ...
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bioRxiv - Genomics 2023Quote: Five µg of each Quartet RNA sample was reverse transcribed using the PrimeScript™ RT reagent Kit (RR037A, TaKaRa) in a 50 µl reaction ...
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bioRxiv - Microbiology 2023Quote: ... Sequencing libraries were prepared by the University of Minnesota Genomics Center using the SMARTer Pico Mammalian V2 kit (Takara). For RNA-Seq from EVTs and STBs ...
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bioRxiv - Genetics 2023Quote: ... Library was prepared following the protocols provided by the manufacturer in SMART-Seq mRNA LP kit (Takara Bio #634768) and Unique Dual Index Kit (U001-U024 ...
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bioRxiv - Cell Biology 2023Quote: ... double-stranded cDNA was synthesized and amplified using the SMARTer Universal Low Input RNA Kit protocol (Clontech, No. 634938), a product optimized for archived biosamples.29 The cDNA was sheared by Covaris ...
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bioRxiv - Biophysics 2023Quote: ... All remaining unsorted cells in addition to the sorted cell populations were then miniprepped (Takara NucleoSpin Plasmid miniprep kit), and DNA was stored at 4 °C short-term or at −20 °C for long-term storage.
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bioRxiv - Biophysics 2023Quote: ... Viral vector titers were determined by the number of DNase I resistant vg using AAV titration kit (TaKaRa/Clontech) by qPCR (StepOne ...
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bioRxiv - Biophysics 2023Quote: ... Viral vector titers were determined by the number of DNase I resistant vg using AAV titration kit (TaKaRa/Clontech) by qPCR (StepOne ...
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bioRxiv - Immunology 2023Quote: ... S-IgA was purified by two-step chromatography using the Capturem His-Taged Purification Kit (Takara Bio, Shiga, Japan) followed by size exclusion chromatography (Cytiva ...
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bioRxiv - Cancer Biology 2023Quote: Real-time PCR was carried out using a TB Green Premix Ex Taq (Tli RNaseH Plus) kit (Takara, Japan) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: The plasmids of TiCGSCy mutants (E1442Q, E1442A and E1356A) were constructed using a PrimeSTAR mutagenesis basal kit (Takara Bio) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... The mutant 2X FLAG TANGO2 Gly154Arg was generated with the In-Fusion HD Cloning Plus Kit (Takara Bio Europe) following the manufacturer’s instructions using the pMK-T plasmid as template ...
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bioRxiv - Microbiology 2023Quote: ... total RNA was collected using a CellAmp Direct RNA Prep Kit for RT-PCR (Real Time) (TaKaRa, Cat# 3732). GAPDH and vRNA levels were measured with a RT-qPCR assay using the One Step TB Green PrimeScript PLUS RT-PCR Kit (Perfect Real Time) ...
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bioRxiv - Immunology 2024Quote: ... Total RNA Sequencing libraries were prepared with SMARTer® Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian (TaKaRa) according to the manufacturer’s protocol ...
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bioRxiv - Systems Biology 2024Quote: The pool of duplets was ligated to each of the barcoded promoter vectors using Takara ligation kit version 2.1 (#6022; Takara). Ligation products were purified using magnetic bead purification and 2 µl of the ligation were electroporated into 20 µl of electrocompetent e ...
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bioRxiv - Plant Biology 2024Quote: ... RT-qPCR analysis was then performed with 1.0 μl of 5-fold diluted cDNA using a SYBR premixed Ex Taq kit (Takara), and a Light Cycler 480 Real-Time PCR System (Roche ...
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bioRxiv - Neuroscience 2024Quote: ... 1µg of total RNA was mixed with a total volume of 10µl of cDNA synthesis kit (TaKaRa 6110A, Tokyo) consisting of dNTP mixture ...
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bioRxiv - Molecular Biology 2024Quote: ... LTR amplicons or gBlocks™ Gene Fragments were mixed with the pLd2G backbone (gLTR_OUT) and genomic (gLTR_IN) amplicons and joined in a single reaction using In-Fusion® HD Cloning Kit (Takara).
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bioRxiv - Biophysics 2024Quote: ... The resulting product was separated on a 0.75 % agarose gel and the 11.6-kb dsRNA was excised and purified using Agarose Gel DNA Extraction Kit (Takara).
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bioRxiv - Bioengineering 2024Quote: ... PCR products were then isolated through gel electrophoresis and purified using the Nucleospin gel purification kit (Takara Bio,740609.250). Linear DNA fragments were then ligated using inFusion assembly and amplified in Stellar competent Escherichia coli (Takara Bio ...
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bioRxiv - Genetics 2024Quote: ... total RNA libraries were prepared by using SMART-Seq v4 Ultra® Low Input RNA Kit for Sequencing (Clontech) according to the manufacture’s instruction and analyzed by Illumina HiSeq 2500 system (Illumina) ...
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bioRxiv - Developmental Biology 2024Quote: ... Sequencing was performed using SMARTer chemistry (SMARTer Ultra Low RNA Kit for the Fluidigm C1 System, Clontech 634835/634935) (three retinae ...
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bioRxiv - Microbiology 2024Quote: Total RNA extracted from homogenized lung tissues was reverse transcribed into cDNA using MiniBEST Universal RNA Extraction Kit (Takara) and PrimeScriptTM RT reagent kit (Takara) ...
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bioRxiv - Microbiology 2024Quote: ... 90 μl of RNase-free water was added and then 2.5 μl of diluted sample was used for real-time RT-PCR according to the manufacturer’s protocol with One step TB green PrimeScript PLUS RT-PCR Kit (Takara) and primers for the nucleocapsid (N ...
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bioRxiv - Immunology 2024Quote: ... 500 ng of total RNA was used for synthesis of cDNA using PrimeScriptTM RT reagent Kit (Takara, Tokyo, Japan). For miRNA expression analysis ...
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bioRxiv - Molecular Biology 2024Quote: ... The ligated RNA was then subjected to TaqMan qPCR using the One Step PrimeScript RT-PCR Kit (Takara Bio), with 200 nM of a TaqMan probe targeting the boundary of the target RNA and the 3′-AD ...
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bioRxiv - Molecular Biology 2024Quote: ... The incubated DNA samples were purified with the NucleoSpin Gel and PCR Clean-up kit (#740609.250; Takara Bio Inc.). The purified DNA samples were used to prepare the library ...
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bioRxiv - Microbiology 2024Quote: ... and all gene cloning operations were performed using an In-Fusion® HD Cloning Kit (Takara Bio, Shiga, Japan). A conjugated pZJD29a vector [65] was employed to clone 500-bp upstream and downstream regions of the target gene ...
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bioRxiv - Molecular Biology 2024Quote: ... and subjected to RT with the use of a TaKaRa PrimeScript II 1st Stand cDNA Synthesis Kit (Takara Bio). The resulting cDNA was subjected to real-time PCR analysis with Fast SYBR Green Master Mix (3485612 ...
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bioRxiv - Genomics 2024Quote: ... the RNA samples were reverse-transcribed to cDNA using the SMART-Seq HT Kit (Takara Bio USA, CA, USA) following the manufacturer’s guidelines ...
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bioRxiv - Zoology 2024Quote: ... Sequencing library fragments in the range of 400–1,000 bp were cut and purified with a NucleoSpin Gel and PCR Clean-up kit (Clontech) and sequenced using a NextSeq500 sequencer with HighOutputMode 75 cycles kit (Illumina) ...
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bioRxiv - Genetics 2024Quote: ... and total RNA was extracted and subsequently used to synthesize cDNA using the TaKaRa RNA PCRTM Kit (AMV) Ver.3.0 (TaKaRa). Quantitative real-time PCR (qPCR ...
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bioRxiv - Genomics 2024Quote: ... High molecular weight gDNA extractions for samples M20 and M29 were generated using the NucleoBond HMW DNA kit (740160.20) from Takara Bio USA ...
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bioRxiv - Genomics 2024Quote: ... The subsequent steps for library preparation were carried out with the DNA Thruplex kit (Takara, Cat. #R400674, Cat. #R400665) following the manufacturer’s protocols.
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bioRxiv - Physiology 2024Quote: ... Total RNA was then extracted from the tissues following the guidelines of the RNAiso Plus kit (Takara, Shiga, Japan). Subsequently ...
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bioRxiv - Developmental Biology 2024Quote: All DNA constructs used in this study were generated by using In-Fusion HD Cloning kit (Takara Bio Inc.). Plasmid mEmerald-N1 was a gift from Michael Davidson (Addgene plasmid #53976) ...
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bioRxiv - Cell Biology 2024Quote: Cell death was assessed by the release of LDH in conditioned medium using LDH Cytotoxicity Detection Kit (TaKaRa, CA). IL-1β levels in conditioned media were measured by an ELISA kit (eBiosciences ...
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bioRxiv - Plant Biology 2020Quote: ... 10 ng of total RNA were amplified and converted to cDNA using SMART-Seq v4 Ultra Low Input RNA kit (Clontech). 17 fmol of cDNA was used for library preparation using the PCR Barcoding kit (SQK-PBK004 kit ...
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bioRxiv - Plant Biology 2019Quote: ... The first-strand cDNA for qRT-PCR was synthesized from 100 ng total RNA using PrimeScript RT reagent Kit with gDNA Eraser (TaKaRa). For qRT-PCR ...
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An mRNA processing pathway suppresses metastasis by governing translational control from the nucleusbioRxiv - Cancer Biology 2021Quote: RNA-seq libraries (used for calculating translation efficiencies) were prepared using SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian (Takara), with 50 ng total RNA as input ...
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bioRxiv - Biochemistry 2021Quote: ... A total of 5 μg of RNA were reverse transcribed and amplified using One Step SYBR Prime-Script PLUS RT-PCR Kit (TaKaRa) and the Thermal Cycler Dice instrument (TaKaRa ...
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bioRxiv - Molecular Biology 2020Quote: ... One µg of total RNA was used as input for SMARTer Stranded Total RNA Sample Prep Kit-HI Mammalian (Clontech). Sequencing was performed on the NextSeq500 instrument (Illumina ...
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bioRxiv - Developmental Biology 2021Quote: Cells were lysed and RNA was reverse transcribed and amplified according to the SMARTer Ultra Low RNA Kit for Illumina Sequencing (version 1, Clontech). Libraries were prepared from cDNA using the NEBNext Ultra DNA library preparation for Illumina sequencing with indexed adaptors (New England Biolabs) ...
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bioRxiv - Cell Biology 2020Quote: ... An RNA library was prepared by JHU Deep Sequencing Core facility beginning with cDNA construction using the SMARTer pico cDNA Synthesis Kit (TaKaRa), followed by ribosomal depletion and library construction using the Illumina TruSeq Stranded Total RNA Library Prep Kit (Illumina) ...