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Citations for Takara Bio :
3201 - 3250 of 5125 citations for Medium Kit without Serum and with CultureBoost since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... Cloning was carried out using the ligation-independent In-Fusion® HD cloning kit (Takara Bio USA, CA). Lentivirus production and transduction were done as described 33 ...
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bioRxiv - Genetics 2022Quote: ... the target fragment of GmXTH1 was amplified according to the SYBR Premix Ex TaqTM Kit (Takara, Otsu, Japan) operation instructions ...
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bioRxiv - Microbiology 2022Quote: cDNA was synthesized from total RNA or immunoprecipitated RNA using PrimeScript RT reagent Kit with gDNA Eraser (Takara). RT-qPCR analysis was performed using TB Green Fast qPCR Mix (Takara ...
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bioRxiv - Microbiology 2022Quote: ... The genes were then cloned into linearized pCMVR backbone using In-Fusion HD Cloning Kit Master Mix (Clontech). Antibodies were produced in Expi293F cells (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... qRT-PCR was performed using the One Step TB Green PrimeScript PLUS RT-PCR Kit (RR096A, TaKaRa, Japan) and a Thermal Cycler Dice Real Time System TP800 (TaKaRa ...
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bioRxiv - Plant Biology 2022Quote: ... 3XHA or 3XFLAG fragments were inserted into the vector using HindIII sites and the In-Fusion kit (Clontech) to generate basic and control effector vectors p35S::HA or p35S::FLAG ...
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bioRxiv - Neuroscience 2022Quote: ... libraries were prepared using the SMARTer® Stranded Total RNA-seq Kit v2 – Pico Input Mammalian (Takara, #634413). Samples were fragmented for 4 min @ 94°C and final amplification was performed using 16 cycles ...
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bioRxiv - Molecular Biology 2023Quote: ... and RNA-sequencing libraries were made using the SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian (Takara). Libraries were sequenced at the Bauer Core Facility (Harvard University ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were sorted directly into buffer for SMART-Seq v4 Ultra Low Input RNA Kit (Clontech Laboratories, Inc.). Subsequent processing was carried out by the UCL Genomics sequencing facility ...
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bioRxiv - Physiology 2022Quote: ... rRNA depleted RNA passing quality control was used in the SMARTer Stranded RNA-Seq Kit (Takara Bio USA). Following RNA incubation with stranded N6 primer ...
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bioRxiv - Neuroscience 2022Quote: ... RNA-seq libraries were generated using the SMARTer Stranded Total RNA-seq kit v2 (Takara Cat no. 634419) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2022Quote: ... Media lactate dehydrogenase (LDH) was assayed by enzymatic colorimetric assay (LDH Cytotoxicity Detection Kit, Takara Bio, cat# MK401).
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bioRxiv - Molecular Biology 2022Quote: ... RNA was reverse-transcribed using a PrimeScript RT reagent Kit with gDNA Eraser (Cat: RR047A, TAKARA, Shiga, Japan), and qPCR was performed on a Bio-Rad CFX96 machine (Hercules ...
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bioRxiv - Cancer Biology 2022Quote: ... USA).and converted to cDNA using a Primescript RT reagent kit (Cat# RR037A, DSS Takara Bio India Private Ltd) as per the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... The PCR product thus obtained was cloned using the In-Fusion HD Cloning Plus kit (Takara Bio – 638909) into the pMA_F3NeoLoxPPolyFRT vector provided by Dr ...
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bioRxiv - Cancer Biology 2022Quote: ... The PCR products were purified from agarose gels using NucleoSpin Gel and PCR Clean-Up Kit (740609, Clontech) and subcloned into the pENTR/D-TOPO vector (K240020 ...
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bioRxiv - Plant Biology 2024Quote: ... Reverse transcription reactions were performed using the PrimeScript II 1st strand cDNA Synthesis Kit (TAKARA, Cat. No. 9750), solution was then removed ...
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bioRxiv - Neuroscience 2024Quote: ... Libraries were prepared using the low-input Clontech SMART-Seq HT with Nxt HT kit (Takara Bio USA) and sequenced using an Illumina NovaSeq 6000 with an S1 100 Cycle Flow Cell (v1.5).
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MALAT1 mediates miR-206/CDC42/PAK1/Paxillin signaling axis to alleviate erectile dysfunction in ratsbioRxiv - Molecular Biology 2024Quote: ... And the obtained RNA was used for reverse transcription using the PrimeScript RT Enzyme Mix I kit (TaKaRa). After the reaction was completed ...
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bioRxiv - Plant Biology 2024Quote: Single or multiple DNA fragments were cloned into binary vector using In-Fusion HD Cloning Kit (Clontech, USA). Briefly ...
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bioRxiv - Plant Biology 2024Quote: ... and inserted into BamHI-digested pGEX-4T-3 by using In-Fusion Smart Assembly Cloning Kit (Takarabio-Clontech). Full-length OcKSL4 cDNA was amplified by RT-PCR using primers 5’-GGATCCATGGCGAATTATCCCATGGAG-3’ (forward ...
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bioRxiv - Synthetic Biology 2024Quote: ... One µg of total RNA was processed using PrimeScript RT reagent Kit with gDNA Eraser (Takara, Beijing, China). Amplified products were sequenced and verified after cloning into a pJET1.2-T vector through CloneJET PCR Cloning Kit (Thermo Scientific ...
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bioRxiv - Zoology 2024Quote: ... One µg of total RNA was processed using PrimeScript RT reagent Kit with gDNA Eraser (Takara, Beijing, China). The PCR procedure was set as follows ...
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bioRxiv - Plant Biology 2024Quote: ... We then performed first-strand synthesis using the SMARTer Stranded Total RNA Kit V2- Pico Input Mammalian (Takara). Following purification with RNAclean XP (Beckman Coulter) ...
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bioRxiv - Plant Biology 2024Quote: ... cDNA was prepared from 1.5 μg of total RNA using the PrimeScript 1st strand cDNA Synthesis Kit (Takara). PCR reactions were performed with 1 μl of a 1:4 dilution of cDNA in ddH2O ...
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bioRxiv - Plant Biology 2024Quote: ... The resulting samples were reverse transcribed into first-strand cDNA using a PrimeScript RT Reagent Kit (Takara Bio). Quantitative PCR (qPCR ...
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bioRxiv - Developmental Biology 2024Quote: Single-cell cDNA synthesis was performed using SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Takara) according to the manufacturer’s protocol with some modifications as previously described17 ...
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bioRxiv - Plant Biology 2024Quote: ... amplified promoter region fragments are inserted with KpnI and XhoI site in pAT006-Fluc-iG5_Rluc-iFAD2_F vector using In-Fusion HD Cloning Kit (Takara). Amplified BOT6 fragment was inserted with pGWB402 using pENTR™ Directional TOPO® Cloning Kits (Invitrogen).
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bioRxiv - Microbiology 2024Quote: ... The resulting RNA was used as input for the SMARTer smRNA-Seq Kit for Illumina (Takara Cat# 635030). cDNA libraries were prepared according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... YCplac22 plasmids that carry the cac1-20 mutant allele were constructed using the PrimeSTAR Mutagenesis Basal Kit (TaKaRa) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... The pmEOS2-C1-ERGIC53 plasmid was constructed using using the In-Fusion® Snap Assembly Cloning Kit (Takara) and transformed into OmniMAX competent cells ...
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bioRxiv - Genetics 2023Quote: ... The purifications for PALS-C steps were done with NucleoSpin Gel and PCR Clean-Up kit (Takara, 740609). Final assembled products were delivered to Endura Electrocompetent Cells (Lucigen ...
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bioRxiv - Cancer Biology 2023Quote: Total RNA was extracted from drug/vehicle-treated cell lines using NucleoSpin RNA kit (Takara, Catalog no. 740955.50). 2 ug of total RNA was used to synthesize cDNA with qScript Ultra Supermix (Quantabio ...
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bioRxiv - Molecular Biology 2024Quote: ... Size selected mRNA was then dephosphorylated and library was prepared using SMARTer smRNA-Seq Kit (TAKARA, cat # 635029) according to vendor’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... Libraries were prepared from Total RNA using the SMART-Seq v4 Ultra low Input RNA kit (Takara Bio) and quantified using Bioanalyzer (Agilent ...
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bioRxiv - Cell Biology 2023Quote: ... The virus titer was quantified using the AAVpro Titration Kit (for Real Time PCR) Ver.2 (Takara Bio) according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2023Quote: cDNA was synthesized from the total RNA of HapT1 cells using the PrimeScript RT Reagent Kit (Takara Bio). The amounts of Pts and Actb were quantified on a LightCycler 480 (Roche ...
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bioRxiv - Cell Biology 2023Quote: The full-length cDNA expression library was constructed using the SMARTer RACE 5’/3’ Kit (Takara Bio, 634858) according to the manufacturer’s instructions for the In-Fusion SMARTer Directional cDNA Library Construction Kit (Takara Bio ...
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bioRxiv - Cell Biology 2023Quote: ... according to the manufacturer’s instructions for the In-Fusion SMARTer Directional cDNA Library Construction Kit (Takara Bio, 634933). 1µg of total RNA extracted from HapT1 with RNeasy Mini Kit (Qiagen ...
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bioRxiv - Microbiology 2024Quote: ... linearized GPC sequence was gel purified using NucleoSpin Gel and PCR Clean-up kit (Takara, Cat. No. 740609.5) and then purified using Ampure XP beads (Beckman Coulter ...
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bioRxiv - Genomics 2023Quote: ... We prepared the adult brain total RNA using the Clontech SmartSeq v4 Ultra-Low Input RNA Kit (Takara). We prepared libraries with the Nextera XT DNA Library Preparation Kit and 96-Sample Index Kit (Illumina ...
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bioRxiv - Bioengineering 2024Quote: ... The cDNA was generated using the SMART-Seq® v4 Ultra® Low Input RNA Kit (Takara Bio). The final sequencing library was made using the Nextera XT kit (Illumina) ...
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bioRxiv - Bioengineering 2024Quote: ... The two fragments were assembled in a single In-Fusion reaction (In-Fusion HD Cloning Kit, Takara, 639650), and the PCR-derived regions of the resulting plasmids were confirmed by sequencing.
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bioRxiv - Cell Biology 2024Quote: Total RNA was isolated with Trizol (Thermo) and cDNA was synthesized with Prime Script RT Reagent Kit (Takara). Quantitative PCR was performed with a SYBR Green PCR kit (Toyobo ...
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bioRxiv - Pathology 2023Quote: ... Reverse RNA transcription was performed using the PrimeScript RT Reagent Kit with gDNA Eraser (RR047A; Takara Bio, Japan). A 10 μL reaction mix was formulated as follows ...
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bioRxiv - Cell Biology 2022Quote: ... and in-chip reverse transcription PCR were performed using a 3’ DE Chip and Reagent kit (Takara Bio) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... Plasmids coding EGFP-mutants were generated from EGFP-IRES-mCherry plasmid using TaKaRa MutanBEST Kit (TaKaRa, Kusatsu, Japan). pG5egfp reporter vector was modified from the pG5luc vector (GeneBank Accession Number AF264724 ...
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bioRxiv - Cell Biology 2023Quote: ... and used for library preparation (5-10 ng) using SMART-Seq v4 Ultra Low Input RNA Kit (Clontech). Libraries were sequenced on Novaseq platform (Illumina).
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bioRxiv - Cell Biology 2023Quote: ... RNA-seq libraries were prepared with the SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian (Takara #634875). 75 bp single-end sequencing was performed using an Illumina NextSeq500 system at the CRI at UT Southwestern Sequencing Facility ...
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bioRxiv - Immunology 2023Quote: ... and 1 µg was used for cDNA synthesis with the PrimeScript High Fidelity RT-PCR Kit (Takara, R022B). Of the cDNA ...