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Citations for Takara Bio :
3051 - 3100 of 5125 citations for Medium Kit without Serum and with CultureBoost since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... Viral RNA was amplified using PrimeScript II High Fidelity One Step RT-PCR Kit (Takara Bio, Shiga, Japan) under the following reaction conditions ...
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bioRxiv - Microbiology 2020Quote: ... cDNA was generated following the SMARTer® 5’/3’ RACE kit protocol (Takara Bio, Kusatsu, Shiga Prefecture, Japan).
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bioRxiv - Developmental Biology 2021Quote: ... cDNA synthesis was completed by SMART-Seq™ v4 Ultra™ Low Input RNA kit for sequencing (Clontech). Samples were sequenced on Illumina machines ...
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bioRxiv - Developmental Biology 2020Quote: ... Total RNA was converted to cDNA using PrimeScript RT reagent Kit with gDNA Eraser (RR047A, Takara, Dalian, China). The mRNA expression levels of the Ccl9 ...
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bioRxiv - Developmental Biology 2020Quote: Total RNA was extracted from cells using the NucleoSpin RNA II kit (Clontech Laboratories, Mountain View, CA, USA). The quality and quantity of total RNA were measured by Nanodrop 1000 (Thermo Fisher Scientific) ...
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bioRxiv - Synthetic Biology 2022Quote: Genomic DNA was extracted from sorted T cells using the Macherey-Nagel NucleoSpin Tissue XS kit (Takara #740901.250). DNA encoding the CAR costimulatory domain was amplified from the extracted genomic DNA using the forward primer 5’-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGNNNNNACTGGTTATCACCCTTTA CTGC-3’ (Integrated DNA Technologies ...
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bioRxiv - Plant Biology 2022Quote: Total RNAs were extracted from various tissues using the MiniBEST Plant RNA Extraction Kit (Takara Bio, Beijing, China). First-strand cDNAs were synthesized using TransScript One-Step gDNA Removal and cDNA Synthesis SuperMix (TransGen Biotech ...
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bioRxiv - Molecular Biology 2022Quote: The RNA samples extracted from the fruit flesh were reverse transcribed using the PrimeScript RT-PCR Kit (TaKaRa) and the oligo(dt)20 primer ...
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bioRxiv - Microbiology 2022Quote: ... The first-strand cDNA was synthesized using reverse transcriptase from the PrimeScript RT reagent kit (Takara Biotechnology, Japan) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... were purchased from Biomatik and cloned into BstBI-digested PBRM1-TetO-FUW using the ligation-free In-Fusion HD cloning kit (Takara). The construct was packaged into lentivirus using HEK293T cells and delivered into target cells together with pLenti CMV rtTA3 Hygro (w785-1 ...
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bioRxiv - Neuroscience 2021Quote: RNA from FACS-sorted cells ranging from 3,000 – 10,000 were isolated with the NucleoSpin RNA XS kit (Clontech) with on column DNase digestion according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... The cDNAs used for RT-PCR were synthesized using PrimeScript First-Strand cDNA Synthesis Kits (Takara, Osaka, Japan). RT-PCR cycling conditions were as follows ...
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bioRxiv - Molecular Biology 2020Quote: ... and one μg RNA was converted to cDNA by using the PrimeScript™ RT-PCR Kit (Takara, Japan) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... then into pShuttle-CMV with EcoRV using the In-Fusion HD Cloning Plus System kit (Takara Bio USA). These were linearized with Pme1 and cotransformed with pAdEasy-1 into BJ5183 cells ...
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bioRxiv - Cell Biology 2022Quote: cDNA was generated from single cells in the 96-well plate using the SmartSeq v4 kits (Takara Bio) using 1/4th volume reactions dispensed using a Mantis dispenser (Formulatrix) ...
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bioRxiv - Immunology 2021Quote: ... cDNA was generated from polyadenylated transcripts employing the SMART-Seq v4 ultra low input RNA kit (Takara Bio). RNA and cDNA quality was analysed on a 2100 Bioanalyser (Agilent) ...
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bioRxiv - Biochemistry 2019Quote: ... Lentivirus was produced by transient transfection of HEK 293T cells using the CalPhos mammalian transfection kit (Clontech laboratories) to introduce packaging vectors (VSVg and GAG/POL/Δ8.2 ...
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bioRxiv - Molecular Biology 2019Quote: ... the cDNA was synthesized by reverse transcription using PrimeScript RT reagent kits with gDNA Eraser (Takara, Dalian, China) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2019Quote: ... we cloned Pikp-HMANK-KE (as above) into pGBKT7 using an In-Fusion cloning kit (Takara Bio USA), following the manufacture’s protocol ...
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bioRxiv - Immunology 2019Quote: ... cDNA was generated from 1 μg of RNA using the PrimeScript RT reagent kit with gDNA Eraser (Takara). The mRNA quantifications of target genes were performed by real-time PCR using the SYBR GREEN MIX (Takara) ...
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bioRxiv - Pathology 2020Quote: ... An RNA library was then constructed using the SMARTer Stranded Total RNA-Seq Kit v2 (TaKaRa, Dalian, China). Ribosomal RNA (rRNA ...
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bioRxiv - Cell Biology 2019Quote: ... The amplified products were ligated using ClonTech In-Fusion HD Cloning kit (Takara Bio USA, Mountain View, CA) and the resultant pBS-HsMyo1e construct contained MYO1E CDS flanked by myo1+ 5’ and 3’ UTRs ...
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bioRxiv - Plant Biology 2019Quote: ... Full-length cDNA of LcCHI2 sequence was obtained using a SMART™ RACE cDNA Amplification Kit (Clontech, USA) for 3’ RACE with the primers 5′-CCGACCAGTTCCAATGGGGCT-3′ and 5′-GGCCACGCGTCGACTAGTACTTTTTTTTTTTTTTTTT-3′.
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bioRxiv - Pathology 2019Quote: ... and treated with DNase I following the manufacturer’s instructions (PrimeScript RT reagent Kit with gDNA Erase, Takara, Japan). cDNA synthesized from reverse transcription of RNA samples was used to determine the mRNA expression levels of target genes as well as for quantifying TYLCV accumulation levels at the date indicated ...
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bioRxiv - Microbiology 2019Quote: Cloning via homologous recombination was carried out using the In-Fusion® HD cloning kit (Clontech Laboratories Inc). Primers used can be seen in Table 2.
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bioRxiv - Microbiology 2020Quote: ... Quantitative real-time PCR analysis was performed using the SYBR Premix Ex TaqTM II kit (Takara, Dalian, China). HMGB1 primers were designed using Primier4.1 software and GADPH was used as a reference gene ...
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bioRxiv - Microbiology 2019Quote: ... the total RNA (1 µg) was reverse transcribed by PrimeScript RT reagent Kit with gDNA Eraser (Takara, China). The IL-1β and components of RISC mRNA expression levels of the NA cells were quantified with the SYBR Green qPCR kit (Takara ...
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bioRxiv - Neuroscience 2019Quote: ... Three ng of RNA was used to prepare cDNA using SMART-Seq v4 Ultra Low Input Kit (Takara). Following cDNA preparation ...
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bioRxiv - Evolutionary Biology 2020Quote: ... coli as a DNA template) with the Neo-selection gene by using In-Fusion HD Cloning kit (Takara), and integrated the resulting leuS-Neo DNA into genomes of the tavaborole-resistant cells by using the scarred method for insertion of a nonselectable DNA fragment recombineering protocol with Neo gene as a selectable marker49.
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bioRxiv - Cell Biology 2019Quote: ... approximately 6 μg RNA was applied for 1st-strand cDNA synthesis using PrimeScriptTM RT Reagent Kit (TaKaRa, AK6003) with oligo (dT ...
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bioRxiv - Genomics 2020Quote: ... cDNA was generated via PrimerScriptTM RT Reagent Kit with genomic DNA Eraser (Perfect Real Time) (TaKaRa, Beijing, China). Quantitative real-time PCR analysis (q-PCR ...
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bioRxiv - Microbiology 2019Quote: Bacterial genomic DNA was extracted and purified using the NucleoBond AXG kit (Takara Bio, Inc., Otsu, Shiga, Japan). DNA library preparation was performed using a Rapid Barcoding sequencing kit (SQK-RBK004 ...
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bioRxiv - Plant Biology 2019Quote: ... and converted to RNA-seq libraries using the Low input library prep kit (Clontech, Mountain View, CA, USA). Each library was diluted to 2 nM ...
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bioRxiv - Physiology 2021Quote: Real-time qRT–PCR was performed on cDNA amplified by PrimeScript II 1st Strand cDNA Synthesis Kit (Takara) using GoTaq qPCR (Promega ...
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bioRxiv - Genomics 2021Quote: ... according to the technical manual for PrimeScriptTM First Strand cDNA Synthesis Kit (Takara Bio Inc., Mountain View, CA). Amplification of VH and VL antibody fragments was carried out according to the standard operating procedure (GenScript ...
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bioRxiv - Synthetic Biology 2021Quote: ... The cDNA was synthesized from 1 μg of total RNA using the PrimeScript™ RT-PCR Kit (Takara). Three technical replicates were measured for gene expression levels in each cDNA sample ...
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bioRxiv - Biophysics 2022Quote: ... Unique clones for each COP were sub-cloned in pRH2.2 using the In-Fusion Cloning kit (Takara Bio). Sequence-verified COPs were transformed into E ...
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bioRxiv - Biophysics 2022Quote: ... Resulting fragments were cloned into linearized FM5-mCh or FM5-GFP constructs using In-Fusion Cloning kit (Takara). The resulting plasmids were sequenced to confirm correct insertion.
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bioRxiv - Bioengineering 2021Quote: ... The virus titre was calculated using the ELISA-based Lenti-X™ p24 Rapid Titer Kit (Takara Bio) following the manufacturer’s instructions.
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bioRxiv - Neuroscience 2020Quote: ... #AM9937).The Brp-T2A-QF2w-Hsp70-3XP3-dsRed donor plasmid was constructed using the InFusion HD Kit (Clontech, #638910). Homology arms of ∼1 kb flanking the sgRNA cut site were cloned by PCR using template genomic DNA from the Orlando strain ...
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bioRxiv - Molecular Biology 2021Quote: ... Three μg of total RNA was subjected to reverse transcription using PrimeScript 1st strand cDNA Synthesis Kit (Takara).
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bioRxiv - Cancer Biology 2020Quote: ... Library construction was performed using the SMARTer® Stranded Total RNA-Seq Kit v2 – Pico Input Mammalian (Takara), and paired-end sequencing was performed on the NovaSeq 6000 instrument (Illumina ...
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bioRxiv - Neuroscience 2020Quote: ... Plasmid DNA to generate viral vectors was prepared using the NucleoBond Xtra Maxi endotoxin free kit (Takara Bio) prior to production ...
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bioRxiv - Neuroscience 2020Quote: cDNA libraries were made by the OHSU Massively Parallel Sequencing Shared Resource using the SMARTer RNA kit (Clontech). Library quality was assessed using a TapeStation 220 and libraries were quantified by qPCR using a KAPA Library Quantification kit ...
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bioRxiv - Plant Biology 2021Quote: ... Total RNA was extracted using the Mini BEST Plant RNA Extraction Kit (Takara Biotech Co., Ltd., Dalian, China) and cDNA was synthesized using the one-step PrimeScript RT-PCR Kit (Takara ...
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bioRxiv - Cell Biology 2021Quote: ... Protein concentrations were determined via the Bradford method using the TaKaRa Bradford Protein Assay Kit (Takara Bio, T9310A).
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bioRxiv - Plant Biology 2020Quote: ... using the HindIII and XbaI sites with the In-Fusion HD Cloning Kit (Takara Bio, Otsu, Shiga, Japan). The construct was verified by sequencing and used to transform Ler by the floral dip method (Clough and Bent ...
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bioRxiv - Plant Biology 2021Quote: ... and was reverse transcribed by the PrimeScriptTM RT reagent Kit with gDNA Eraser following the manufacturer’s instructions (TaKaRa). RT-qPCR was performed with SYBR Green mix (Bimake) ...
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bioRxiv - Microbiology 2020Quote: ... Pseudovirus-containing supernatants were collected 48 h later and concentrated using Lenti-X™ concentrator kit (Takara, CA). Pseudovirus neutralization assay was then performed by incubation of SARS-CoV-2 pseudovirus with 5 folds serially diluted antibody with starting concentration of 1 μg/ml for 1 h at 37 °C ...
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bioRxiv - Neuroscience 2020Quote: ... reverse transcription and amplification was achieved using the SMART-Seq v4 ultra low input RNA Kit (Takara, 634891). This kit improves synthesis of the full-length cDNA via a template switching mechanism for synthesis of the second strand cDNA ...