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Citations for Takara Bio :
3351 - 3400 of 5125 citations for Medium Kit without Serum and with CultureBoost since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2020Quote: ... This was followed by a nested PCR and cloning of the products using the Mighty TA-cloning kit (TaKaRa). Twenty independent clones were randomly picked and sequenced.
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bioRxiv - Plant Biology 2020Quote: Ptr1 candidate genes were cloned from LA4277-Ro (Ptr1/Ptr1) cDNA into pBTEX (Table S7) using the In-fusion cloning kit manufacturer’s instructions (Takara). StPtr1 was cloned from Dakota Crisp potato plants (Solanum tuberosum ...
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bioRxiv - Plant Biology 2020Quote: ... as a gBlocks fragment and cloned into pBTEX using the In-fusion cloning kit following the manufacturer’s instructions (Takara).
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bioRxiv - Plant Biology 2020Quote: ... StPtr1 was cloned from Dakota Crisp potato plants (Solanum tuberosum) into pBTEX via the In-fusion cloning kit (Takara). Nucleotide and amino acid sequences have been deposited in GenBank for Ptr1 from S ...
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bioRxiv - Microbiology 2021Quote: ... The PCR product of the assembled sequence was purified with a DNA Fragment Purification Kit (Takara, Dalian of China) and eluted with ddH2O ...
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bioRxiv - Microbiology 2021Quote: ... Sequencing libraries were then prepared using SMARTer Stranded Total RNA-Seq - Pico Input Mammalian Kit (Takara Bio, Kyoto, Japan) according to manufacturers protocols ...
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bioRxiv - Neuroscience 2020Quote: ... Libraries were constructed using the SMART-seq v4 Ultra Low-input RNA sequencing kit with Nextera XT (Takara Bio). Paired-end sequencing was conducted by the UCLA genomic core facility (https://www.semel.ucla.edu/ungc/services) ...
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bioRxiv - Developmental Biology 2021Quote: ... Messenger RNAs were reverse transcribed and amplified using the SMART-Seq V4 ultra low input RNA kit (Clontech, France) according to the manufacturer recommendations ...
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bioRxiv - Developmental Biology 2020Quote: ... 5’-RACE and 3’-RACE reactions were performed with the Smart RACE cDNA Amplification Kit (Clontech, Palo Alto, CA) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2020Quote: ... RNAiso™ reagent and PrimeScript ® RT reagent Kit With gDNA Eraser (Perfect Real Time) were purchased from Takara Bio Inc ...
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bioRxiv - Neuroscience 2021Quote: ... Sequencing libraries were prepared from the amplified cDNA using SMART-Seq PLUS kits (Takara Bio USA, Mountain View, CA). Unique dual indexes were used on the amplified libraries to identify samples ...
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bioRxiv - Neuroscience 2021Quote: ... High quality samples from matched input and IP fractions were prepared using the (Low-input Library Prep kit (Clontech). Sequenced reads were mapped with STAR aligner (Dobin et al. ...
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bioRxiv - Cell Biology 2020Quote: RNA libraries from the bulk tissues were prepared using SMART-Seq v4 Ultra Low Input RNA Kit (Clontech, 634888), with a 15 PCR cycles for the amplification step ...
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bioRxiv - Cell Biology 2020Quote: ... Each capture section was visually confirmed by focusing the PALM on the targeted adhesive cap after the collection session and immediately resuspend in 10.5µl of 1x reaction buffer from SMART-Seq v4 Ultra Low Input RNA Kit (Clontech, 634888). Tissue lysis was achieved by incubation for 5min at room temperature and stored at −20°C ...
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bioRxiv - Neuroscience 2020Quote: ... The resulting input and neuronal IP samples were used for isolation of total RNA using Nucleospin RNA XS kit according to manufacturer’s protocol (Takara).
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bioRxiv - Cancer Biology 2021Quote: ... The libraries were prepared using the SMART-Seq® v4 Ultra® Low Input RNA Kit for Sequencing (TaKaRa) followed by Nextera XT (Illumina) ...
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bioRxiv - Cell Biology 2021Quote: ... The point mutants of Kif7-CC were generated by site-directed mutagenesis using In- Fusion HD cloning kit (Takara). The Kif7 SCC construct (aa488-540 ...
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bioRxiv - Microbiology 2020Quote: ... or directly cloned into linearized CS-CA-MCS (VLT-ORF63a and VLT-ORF63b) using In-Fusion HD Cloning kit according to the manufacturer’s instruction (Clontech). The primer sets used for cDNA cloning into the CS-CA-MCS plasmid are listed in Supplementary Table 4 ...
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bioRxiv - Plant Biology 2020Quote: ... Approximately 2 μg of total RNA was reverse-transcribed using PrimeScript™ RT reagent Kit with gDNA Eraser (TaKaRa). Real-time quantitative RT-PCR was performed with TB Green Premix Ex Taq™ (TakaRa ...
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bioRxiv - Plant Biology 2020Quote: ... qRT-PCR reactions were performed with gene-specific forward and reverse primers using the PrimeScript RT-PCR kit (TaKaRa) on the StepOne Real-Time PCR system (Applied Biosystems) ...
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bioRxiv - Plant Biology 2021Quote: ... and cloned into the XbaI digestion site of pMpGWB301 using In-Fusion HD Cloning Kit (Takara Bio, Shiga, Japan). For construction of proMpYUC2:MpCLE1 ...
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bioRxiv - Neuroscience 2020Quote: ... and the sequencing library was prepared with SMARTer Stranded Total RNA-Seq Kit v2-Pico Input (Takara, Shiga, Japan), and sequenced with Illumina HiSeq 2500 ...
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bioRxiv - Plant Biology 2021Quote: ... and first-strand cDNA was synthesized from 500 ng total RNA using the PrimeScript RT reagent Kit (Takara, Japan). Semi-quantitative RT-PCR was performed using PrimeSTAR GXL (Takara ...
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bioRxiv - Cancer Biology 2020Quote: 1μg of RNA was used for conversion of cDNA from each sample using PrimerScript cDNA conversion kit (TAKARA, Japan), following manufacturers’ protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... Ribo-seq libraries were then prepared with the purified footprints and the SMARTer Small RNA-Seq Kit (Takara, 635029) following the manufacturer’s instructions.
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bioRxiv - Cell Biology 2021Quote: ... Libraries were prepared using the SMARTer Stranded Total RNA-seq Kit v2 – Pico Input mammalian (Takara Bio, CA, USA) according to manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... SYBR Green-based RT-qPCR were used according to the manuals by SYBR Premix Ex Taq kit (Takara Bio). Primers used in RT-qPCR reactions as follows ...
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bioRxiv - Cancer Biology 2021Quote: Libraries were prepared using the SMARTer Stranded Total RNA-Seq v2-Pico Input Mammalian Kit (Takara Bio USA, #634415) according to the instructions of the manufacturer ...
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bioRxiv - Cell Biology 2021Quote: All constructs were made using traditional cloning, Gibson assembly (Gibson et al., 2009) or In-Fusion kit (Takara Bio); for full details and Gene Accession numbers ...
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bioRxiv - Cell Biology 2021Quote: ... Total RNA (1 μg) was converted to cDNA using the PrimeScript™ RT reagent Kit with gDNA Eraser (TAKARA). Quantitative PCR (qPCR ...
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bioRxiv - Cell Biology 2020Quote: ... cDNA was synthesized using a PrimeScript II High Fidelity One Step RT-PCR Kit (R026A, Takara Bio, Shiga, Japan), purified using a QIAquick PCR Purification Kit (QIAGEN ...
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bioRxiv - Cancer Biology 2022Quote: ... The SMARTer Stranded Total RNA-Seq Kit - Pico Input Mammalian (Clontech Laboratories, Inc., A Takara Bio Company,California, USA) was used in the succeeding steps ...
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bioRxiv - Microbiology 2022Quote: ... The 5’ end regions of MIC14 and MIC15 were amplified using the SMART RACE cDNA Amplification Kit (Clontech BD) using total or poly(A)+tachyzoite RNA (strain RH ...
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bioRxiv - Neuroscience 2022Quote: ... CquiOR27 and CquiOR28 were subcloned separately from pGEM-T-CquiOR27 and pGEM-T-CquiOR28 into pGEMHE using In-Fusion HD Cloning Kit according to the manufacturer’s instructions (Clontech). PCR In-Fusion primers were designed according to the user manual ...
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bioRxiv - Bioengineering 2022Quote: HEK293T cells were transfected with given plasmids and then harvested for RNA extraction by NucleoSpin RNA Plus Kit (Takara) 48 hour after transfection ...
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bioRxiv - Molecular Biology 2022Quote: ... The BAC containing Idh was extracted from 200 ml bacterial cultures using the NucleoBond Xtra Midi kit (Takara Bio). The fluorescence in situ hybridization (FISH ...
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bioRxiv - Molecular Biology 2022Quote: ... qRT-PCR was carried out by using TB Green Premix Ex Taq II (Tli RNaseH Plus) kit (Takara, China) on ABI7500 Real-time system (Applied Biosystems ...
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bioRxiv - Neuroscience 2022Quote: ... a sequence region of 5,000 bp between SnaBI and AgeI restriction sites was amplified using the HiFi amplification kit (Clontech, Takara Bio Europe ...
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bioRxiv - Molecular Biology 2022Quote: ... Splice sites (SS) mutations were introduced in the corresponding parental plasmid using In-Fusion HD Cloning kit (Takara Bio) to generate pcDNA3.1-RLuc_FOXP3 Intron 7 5’-SS G- U ...
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bioRxiv - Molecular Biology 2022Quote: The quantified RNA was converted into copy DNA (cDNA) using Primescript™ 1st strand cDNA synthesis kit (TaKaRa, Japan). 1st Reaction Mixture was prepared by adding 8.0 µl of RNA (1.0μg/concentration adjusted to 125.0ng/µl) ...
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bioRxiv - Bioengineering 2022Quote: ... A lentiviral transfer plasmid co-encoding zsGreen with Oatp1b1 was cloned using the In-Fusion HD Cloning kit (Takara Bio USA Inc ...
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bioRxiv - Developmental Biology 2022Quote: ... The RNA-seq library was prepared with SMARTer® Stranded Total RNA-Seq Kit v2 – Pico Input Mammalian (Takara), and sequencing was performed in the Next Generation Sequencing platform using NextSeq500 (Illumina) ...
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bioRxiv - Immunology 2022Quote: The sequence of anti-IFNγ was cloned from XMG1.2 hybridoma (ATCC) using SMARTer RACE 5’/3’ kit (Takara Bio). The 6xHis tagged anti-Dsg ...
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bioRxiv - Microbiology 2022Quote: ... The cDNA was prepared from 1 μg of total RNA using PrimeScript 1st strand cDNA Synthesis Kit (TaKaRa, Japan) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2022Quote: ... RNA was reverse transcribed to cDNA using the PrimeScript™ RT reagent Kit with gDNA eraser (Takara, Kusatsu, Japan) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... The cDNA was amplified with gene-specific primers (Supplemental Table 1) and SYBR Premix Ex Taq II kit (TaKaRa). Data were analyzed using a 2−ΔΔCt method.
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bioRxiv - Cancer Biology 2022Quote: ... Forty ng cDNA was used as templates for RT-PCR using SYBR® Premix Ex Taq kit (TaKaRa, #RR820A) using LightCycler 96 (Roche ...
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bioRxiv - Cancer Biology 2022Quote: ... RNAseq libraries were made using the SMARTer ultra-low RNA kit V3 (Takara Bio USA, Mountain View, CA, USA). and analysed individually (i.e. ...
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bioRxiv - Cancer Biology 2022Quote: Four hundred nanogram total RNA was employed for cDNA synthesis using ProtoScriptII First Strand cDNA Synthesis kit (TaKaRa,#RR036A) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: To eliminate nucleotides that are part of the template-switching oligo as per the manufacturer’s instructions (SMARTer Stranded Total RNA-Seq Kit - Pico Input Mammalian; v1 [mPFC] or v2 [HIP]; Takara), the first three nucleotides of the first sequencing read (Read 1 ...