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Citations for Takara Bio :
3101 - 3150 of 3947 citations for hsa let 7f RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... gel purified using the Nucleospin Gel and PCR Clean-Up Kit (TaKaRa Bio Inc., Kusatsu, Japan), and then cloned into the XbaI site of pHIV7/PGK-neo using InFusion cloning (TaKaRa Bio Inc. ...
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bioRxiv - Immunology 2022Quote: ... and treated with Exonuclease I (New EnglandBiolabs) before amplification by the Advantage 2 PCR kit (Clontech) and the SINGV6 primer (95°C for 1 min ...
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bioRxiv - Neuroscience 2023Quote: ... 5 μl of the PCR product was treated with 2 μl cloning enhancer (Takara-bio Inc.). The cloning reaction was prepared by adding 2 μl of 5X In-Fusion HD Cloning enzyme mix ...
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bioRxiv - Cancer Biology 2023Quote: ... a region spanning the ENE domain was amplified by conventional PCR (PrimeStar HS mix, Takara Bio) with primer sequences listed in Table S9 ...
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bioRxiv - Molecular Biology 2023Quote: ... qRT-PCR was performed using the Thermal Cycler Dice Real Time System (Takara Bio, Kusatsu, Japan). Primers and templates were mixed with TB Green Premix Ex Taq II (Takara Bio) ...
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bioRxiv - Cell Biology 2023Quote: ... Quantitative real-time PCR was performed in Thermal Cycler Dice Real Time System Lite (Takara Bio) using KOD SYBR qPCR Mix (Toyobo ...
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bioRxiv - Plant Biology 2023Quote: ... The full-length CAMTA3 cDNA was PCR amplified using the HS Primestar DNA polymerase (Takara, USA) with forward and reverse primers bearing BamHI and XhoI ...
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bioRxiv - Genomics 2023Quote: ... Amplified oligonucleotides were purified using the NucleoSpin PCR clean-up and gel extraction kit (Takara Bio). CROP-seq-opti was linearized via digestion with BsmBI and alkaline phosphatase (NEB ...
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bioRxiv - Genomics 2023Quote: ... while the out/out PCRs were carried out with PrimeSTAR GXL DNA Polymerase (Takara Bio, R051A). Seamless HDR was confirmed by sequencing the junctions between the insert and the mouse genome ...
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bioRxiv - Immunology 2023Quote: ... qRT-PCR was performed using the SYBR® Premix Ex Taq™ II (Takara, Shiga, Japan) Kit and a StepOnePlus Real-Time PCR instrument ...
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bioRxiv - Immunology 2023Quote: ... The viral copies were quantitated by reverse transcription quantitative PCR following the handbook (Takara, Cat.#RR086A). The copy numbers of reverse-transcripts from cell cultures and mice organ samples were normalized with the expression level housekeeping gene of beta-actin by using the 2(-Delta Delta C(T) ...
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bioRxiv - Cancer Biology 2023Quote: ... cDNA was generated from the adapter ligated RNA using the SMARTer PCR cDNA Synthesis Kit (Clontech), replacing the CDS Primer IIA with a custom primer complementary to the 3′ end adapter for first strand synthesis (TableS6) ...
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bioRxiv - Cancer Biology 2023Quote: ... PCR for selection cassette production was performed with SeqAmp DNA Polymerase (Takara Bio, cat. no. 63850) using the ultramers as primers and AAT-PB-CD2APtk (Addgene #86004 ...
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bioRxiv - Developmental Biology 2023Quote: ... PCR condition modifications to amplify RACE products are as followed using SeqAmp DNA Polymerase (Takara Bio): Fat1 (TA ...
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bioRxiv - Cell Biology 2023Quote: ... Viral titer was quantified using the Lenti-X™ qRT-PCR Titration Kit (Takara Bio, #631235), according to manufacturer’s protocol.
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bioRxiv - Cell Biology 2023Quote: ... Second strand synthesis and PCR amplification was performed by adding the Advantage 2 Polymerase Mix (Clontech) and the SINGV6 primer (10 pmol ...
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bioRxiv - Evolutionary Biology 2023Quote: ... PCR was performed using TaKaRa PrimeSTAR GXL DNA Polymerase following the manufacturer’s protocol (Takara Biotechnology, Japan). Amplified DNA fragments were purified by illustra ExoProStar (GE Healthcare Life Sciences) ...
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bioRxiv - Plant Biology 2023Quote: The truncated version of the RPF2nad6 was generated by PCR amplification using Takara PrimeStar polymerase (TaKaRa) and a primer pair designed to remove the last 180 nucleotides of the RPF2nad6 coding sequence representing the RfCTD sequence (Supplementary table 6) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and deposited into a PCR tube with 5 μL lysis buffer (Takara Bio, San Francisco, CA). cDNAs were then prepared by reverse transcriptase (Takara Bio ...
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bioRxiv - Microbiology 2023Quote: ... Amplifying PCR of the first stand cDNA product was then performed using SeqAmp DNA Polymerase (Takara) with a nested 3’ primer to the constant genes and a 5’ universal primer based on universal primer sites added to the 5’ end during cDNA generation ...
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bioRxiv - Molecular Biology 2023Quote: ... The first step of cDNA synthesis was done using the SMARTer PCR cDNA Synthesis Kit (Takara). Then ...
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bioRxiv - Biochemistry 2024Quote: The SUGCT coding sequence was amplified by PCR by using PrimeStar GXL polymerase (Takara Bio Inc) using the following primers (start and stop codon in bold) ...
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bioRxiv - Plant Biology 2024Quote: ... and amplified by PCR using Takara Premix Ex TaqTM Hot Start Version (Takara Research, RR030A, Japan). The amplified products were recovered from an agarose gel using a Universal DNA Purification Kit (TIANGEN ...
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bioRxiv - Cell Biology 2024Quote: The PCR mixtures for the reactions described here were composed using PrimeStar (Takara Bio, Shiga, Japan) with a 2X GC buffer ...
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bioRxiv - Plant Biology 2024Quote: ... The obtained cDNA was subjected to 5’ RACE-PCR using SeqAmp DNA Polymerase (Takara Bio USA). Primers used for 5’ RACE-PCR and the number of PCR cycles are shown in Supplemental Table 3 ...
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bioRxiv - Synthetic Biology 2024Quote: ... DNA fragments were amplified using PCR using PrimeSTAR® Max DNA Polymerase (Takara Biomedical Technology (Beijing) Co. ...
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bioRxiv - Developmental Biology 2024Quote: ... Full-length cDNA fragment of human MAK was amplified by PCR using human retinal cDNA (Clontech) as a template and subcloned into the pCAGGSII-2xHA vector (62) ...
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bioRxiv - Cell Biology 2020Quote: ... FAM111A cDNAs were PCR amplified and inserted into destination vectors using In-Fusion seamless cloning (Takara Bio). The pTRIPz-MycBioID vector allows for doxycycline-inducible expression of RNF4 or FAM111A with N-terminal fusion of a Myc tag and a promiscuous biotin ligase (MycBioID ...
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bioRxiv - Developmental Biology 2021Quote: ... 2015) with MluI and inserting PCR-amplified EGFP from pEGFP-C1 (Clontech Laboratories, Mountain View, CA, USA). Tol2-mCherry was produced by digesting Tol2-EGFP-C1 with NheI/EcoRI to remove GFP and inserting mCherry amplified by PCR from 8xGliBS-IVS2-mCherry-NLS-polyA-Tol2 (a gift from James Chen (Addgene plasmid #84604) ...
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bioRxiv - Microbiology 2019Quote: ... Amplifications were typically performed in 20 μL reaction volumes containing 1× EmeraldAmp GT PCR Master Mix (Takara Bio Inc. ...
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bioRxiv - Evolutionary Biology 2021Quote: ... or with standard (DreamTaq - Thermo Fisher Scientific, or EmeraldAmp Max HS PCR Master Mix - TaKaRa Bio Inc) DNA polymerases ...
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bioRxiv - Plant Biology 2020Quote: ... The qRT-PCR reaction mixture included TB Green® Premix Ex Taq™ II (Takara, Shiga, Japan). The qRT-PCR conditions were as follows ...
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bioRxiv - Neuroscience 2021Quote: The sgRNA sequences were amplified using Guide-it™ CRISPR Genome-Wide Library PCR Kit (Takara, 632651) and subjected to the high-throughput amplicon sequencing on NextSeq500 ...
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bioRxiv - Genomics 2019Quote: ... 1 ng full length cDNA was used to perform 35-cycle PCR with Premix Taq™ (TaKaRa). PCR products were purified with QIAquick Gel Extraction Kit (Qiagen ...
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bioRxiv - Neuroscience 2020Quote: ... PCR amplification of the vectors and the FPs was performed using CloneAmp™ DNA polymerase (Clontech, USA). The PCR products were gel purified (Macherey-Nagel ...
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bioRxiv - Microbiology 2019Quote: ... Outside-in colony PCR of a subset of hpn genes was performed with PrimeSTAR DNA polymerase (TaKaRa) using the following primers:
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bioRxiv - Biochemistry 2020Quote: ... were replaced by the BG505-NxT or BG505-NxS PCR fragments using the In-Fusion enzyme (Clontech) as described by the manufacturer ...
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bioRxiv - Pathology 2019Quote: ... An aliquot of the cDNA was mixed with 25 µL SYBR® Green PCR Master Mix (TaKaRa) and 10 pmol of each specific forward and reverse primer ...
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bioRxiv - Microbiology 2019Quote: ... the cDNA was purified and eluted in 20ul of elution buffer (NucleoSpin PCR Clean-up Kit, Clontech). The immunoglobulin PCRs were set up with Platinum Taq High-Fidelity DNA Polymerase (Life Technologies ...
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bioRxiv - Evolutionary Biology 2019Quote: Templates for mRNA in situ hybridization probes were cloned by PCR or SMARTer 3’/5’-RACE (Clontech) from cDNA or genomic DNA (see Supplemental File 1 for details) ...
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bioRxiv - Developmental Biology 2019Quote: ... The PCR products were extracted from agarose gel using MiniBEST Agarose Gel DNA Extraction Kit (Takara, Japan) based on manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... DNA was amplified from WT genomic DNA by PCR using Advantage 2 Polymerase (#639202, Takara Bio, USA). Plasmid backbones were double-digested with the necessary restriction enzymes and purified using the Qiagen DNA purification kit (#28704X4 ...
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bioRxiv - Genomics 2020Quote: ... PCR reactions were carried out according to manufacturer’s instructions using either ExTaq polymerase (TaKaRa Bio, Tokyo, Japan) or PCR master mix (Promega ...
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bioRxiv - Microbiology 2021Quote: ... RNA-free cDNA (5μL) was used as a template for PCR with PrimeStar GXL Polymerase (Takara, Japan) and back-to-back PCR primers (LigSeq-F and LigSeq-R ...
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bioRxiv - Neuroscience 2020Quote: ... The lentiviral genome copy number was calculated using the Clontech Lenti-X qRT-PCR Titration kit (Takara).
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bioRxiv - Plant Biology 2021Quote: ... qRT-PCR was performed by TB Green® Premix Ex Taq™ □ (Tli RNaseH Plus) (TAKARA, Japan) on the CFX96 Touch™ Real-Time PCR Detection System (BIO-RAD ...
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bioRxiv - Cell Biology 2021Quote: ... Slc37a2 was amplified by PCR from BMMCs cDNA and inserted into a plEGFP-C1 plasmid (Takara Bio). Slc37a2 plEGFP-C1 plasmid or plasmid vector control were transfected into Amphopack-293 cells (BD Biosciences ...
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bioRxiv - Cell Biology 2021Quote: ... Q-PCR was carried out using the SYRB Premix Ex Taq™ Perfect Real-Time system (Takara). The expression levels were normalized to that of the housekeeping gene GADPH ...
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bioRxiv - Microbiology 2020Quote: ... The synthetic sequence was cloned into inversely amplified pSW002-Pc PCR product by infusion cloning (Takara, Z9633N). pSW002-Pc was a gift from Rosemarie Wilton (Addgene plasmid # 108234 ...
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bioRxiv - Microbiology 2020Quote: ... the genomic DNA served as a template for PCR with Tks Gflex DNA polymerase (Takara Bio Inc.) using the following primer sets ...