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Citations for Takara Bio :
3051 - 3100 of 4901 citations for Uridine Triphosphate UTP CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... First strand cDNA synthesis was performed on 200ng RNA using the SMARTer PCR cDNA Synthesis Kit (Clontech, UK) according to manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... Libraries were prepared using the SMARTer Stranded Total RNA-Seq kit v2 - Pico Input Mammalian (Clontech, TaKaRa Bio). Unfragmented RNA was reverse transcribed with adapter-linked random hexamers and the first strand cDNA was then converted into double-stranded dual-indexed DNA libraries ...
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bioRxiv - Microbiology 2023Quote: ... 5’RACE was performed with SMARTer RACE 5’/3’ Kit (Takara Bio USA, Inc. San Jose, CA USA) according to the manufacturer’s directions.
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bioRxiv - Cell Biology 2023Quote: ... Sequencing libraries from treated RNA were created using the SMARTer Stranded Total RNA HI Mammalian kit (Takara 634873) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: All libraries were prepared using the SMARTer Stranded Total RNaseq Kit v2 – Pico Input Mammalian Components (634419, Takara) according to manufacturer’s protocols and barcoded (634452 ...
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bioRxiv - Genomics 2023Quote: ... Libraries were prepared using the SMARTer Stranded Total RNA-Seq kit v2 - Pico Input Mammalian (Clontech, TaKaRa Bio). Unfragmented RNA was reverse transcribed with adapter-linked random hexamers and the first strand cDNA was then converted into double-stranded dual-indexed DNA libraries ...
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bioRxiv - Immunology 2023Quote: ... Genomic DNA was isolated from tail biopsies using the NucleoSpin Tissue kit (TaKaRa Bio USA, San Jose, CA) following manufacture protocol ...
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bioRxiv - Microbiology 2023Quote: ... Reverse transcription was performed to make cDNA using PrimeScript RT reagent kit with gDNA Eraser (TaKaRa, Kyoto, Japan). Sequences encoding the C-terminal portion (amino acid 45 to 131 ...
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bioRxiv - Immunology 2023Quote: ... Total RNA was normalized prior to oligo-dT capture and cDNA synthesis with SMART-seq v4 kit (Takara). The resulting cDNA was quantitated using a Qubit 3.0 fluorometer ...
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bioRxiv - Molecular Biology 2023Quote: ... The 5’ and 3’ ends of the cDNA were amplified using the SMARTer RACE cDNA Amplification Kit (Clontech) according to the manufacturer’s guidelines ...
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bioRxiv - Bioengineering 2023Quote: ... Purified insert DNA was cloned into the linearized modRNAc1 plasmid using the In-Fusion Cloning Kit (Takara Bio). The DNA template for modRNA synthesis was PCR amplified from the successfully cloned modRNAc1 plasmid followed by PCR purification using DNA Clean & Concentrator-5 (Zymo Research) ...
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bioRxiv - Biochemistry 2023Quote: ... Genomic DNA from WT and Strt1−/− cells was extracted with a NucleoSpin Tissue kit (Takara Bio, Shiga, Japan) and subjected to PCR using specific primers (Oligonucleotide sets 3 ...
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bioRxiv - Bioengineering 2023Quote: ... The synthesized mRNA was subsequently purified using a NucleoSpin® RNA Clean-Up Kit (Takara Bio Inc., Japan). The concentration of the purified mRNA was quantified using a Nanodrop One (Thermo Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... Dox-inducible vectors were generated according to a publicly available protocol54 using In-Fusion HD Cloning Kit (Clontech). Vectors were amplified in Stellar Competent Cells (Clontech ...
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bioRxiv - Biophysics 2023Quote: ... The first-strand cDNA was reverse transcribed with PrimeScript™ RT Reagent Kit with gDNA Eraser (TaKaRa, Japan). Quantitative real-time PCR was run on a Roche LightCycler® 480 II real-time PCR detection system ...
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bioRxiv - Plant Biology 2023Quote: ... was performed to determine the expression level of transcripts utilizing the SYBR Green PCR master mix kit (Takara). The unique region of transcripts was selected for designing qRT-PCR primers and mentioned in Table S3 ...
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bioRxiv - Genetics 2024Quote: ... These three fragments were cloned into EcoRI-digested pBluescript-II vector using In-Fusion HD cloning kit (Takara). gRNA plasmids were constructed by cloning of annealed oligo DNA (Ti902/903 and Ti904/905 ...
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bioRxiv - Biochemistry 2024Quote: A pull-down assay was performed using a Capturem His-Tagged Purification Miniprep Kit (#635710, Takara, Shiga, Japan). 50 µg of BSA was added to the column for blocking ...
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bioRxiv - Neuroscience 2024Quote: ... Viral titer (p24 core antigen content) was determined initially using a LentiX GoStix Plus titration kit (Takara #631280) and subsequently by HIV p24 AlphaLISA detection kit (PerkinElmer #AL291C) ...
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bioRxiv - Plant Biology 2024Quote: ... This suspension was used to determine total protein concentrations using Takara BCA Protein Assay Kits (TaKaRa, Kusatsu, Japan).
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bioRxiv - Neuroscience 2024Quote: ... strand cDNA was synthetized using the TaKaRa PrimeScipt RT Reagent Kit (Cat# RR037A, Takara Bio Inc., Kusatsu, Japan) using 20 µM Random decamer first strand primers according to the manufactorer’s instructions.
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bioRxiv - Immunology 2024Quote: ... In-fusion assembly with the 2 fragments was performed using the In-fusion snap assembly kit by Takara Bio.
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bioRxiv - Genomics 2024Quote: Sequencing libraries were prepared from isolated RNA using two different kits: Takara SMART-Seq v4 3′ DE (Takara) and Lexogen QuantSeq 3′ (Lexogen ...
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bioRxiv - Cell Biology 2024Quote: ... One microgram of total RNA was subjected to first strand cDNA synthesis using PrimeScript RT Reagent kit (Takara). The target cDNA was amplified using TB Green Premix Ex Taq II (Takara ...
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bioRxiv - Cell Biology 2024Quote: ... One microgram of total RNA was subjected to first strand cDNA synthesis using PrimeScript RT Reagent kit (Takara). The target cDNA was amplified using TB Green Premix Ex Taq II (Takara ...
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bioRxiv - Genetics 2024Quote: ... 1 μg of total RNA was reversely transcribed into cDNA using the Prime Script RT kit (Takara, RR047A). The SYBR Green qPCR master mix (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2024Quote: ... Ligation of the various components was undertaken using an In-Fusion cloning kit (Takara Bio-Europe, Paris, France). The ligation reaction was transformed into Stellar competent cells as per manufacturer protocols ...
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bioRxiv - Genetics 2024Quote: ... Complementary DNA (cDNA) was synthesized from total RNA using a PrimeScript RT reagent Kit (Cat. no. RR047B, TaKaRa) and was used as a template to perform qPCR in TB green-based qPCR instrument (CFX-96 ...
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bioRxiv - Immunology 2024Quote: ... Viral titer (p24 core antigen content) was determined initially using a LentiX GoStix Plus titration kit (Takara #631280) and subsequently by HIV p24 AlphaLISA detection kit (PerkinElmer # AL291C) ...
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bioRxiv - Microbiology 2024Quote: ... Then dual-indexing library preparation was created in equimolar conditions following the SMARTSeq Library Prep kit (Takara Bio) and Illumina sequencing 2x 150 reads was done on a Novaseq SP by Fasteris-GeneSupport SA (Switzerland) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and upstream fragment) were connected using the In-Fusion reaction (In-Fusion HD Cloning Kit, Takara, Shiga, Japan) to construct the LBDG_21500-disrupting plasmid (Table S9).
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bioRxiv - Neuroscience 2024Quote: ... ds-cDNA was prepared using the SMARTer Ultra Low RNA kit for Illumina Sequencing (Takara-Clontech cat# 634936) per manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... ds-cDNA was prepared using the SMARTer Ultra Low RNA kit for Illumina Sequencing (Takara-Clontech cat# 634936) per manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... The viral RNA copy number was standardized using a SARS-CoV-2 direct detection RT-qPCR kit (Takara). Fluorescent signals from resulting PCR products were acquired using a Thermal Cycler Dice Real Time System III (Takara).
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bioRxiv - Neuroscience 2024Quote: ... and 3 μg of RNA was reverse transcribed using PrimeScript™ RT reagent kit (Takara, San Jose, CA), according to manufacturer’s instruction ...
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bioRxiv - Plant Biology 2020Quote: ... The first-strand cDNA was synthesized from genomic DNA-removed RNA by using PrimeScript™ RT reagent kit (TaKaRa). Next ...
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bioRxiv - Immunology 2021Quote: ... 1ng of total RNA was pre-amplified with the SMARTer Ultra Low Input kit v4 (Clontech, Mountain View, CA) per manufacturer’s recommendations ...
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bioRxiv - Immunology 2021Quote: ... The mutated NLRP3-D303N and NLRP3-L353P were generated using the PrimeSTAR Mutagenesis Basal kit (Takara Bio, Shiga, Japan) (Aizawa et al. ...
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bioRxiv - Immunology 2021Quote: ... The first-strand cDNA synthesis was performed with the Prime Script RT Master Mix kit (Takara Bio Europe, France). Quantitative real-time PCR was performed at 60°C using Takyon ROX SYBR MasterMix (Eurogentec ...
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bioRxiv - Immunology 2021Quote: ... was used to for two-step qPCR assay after cDNA synthesis using PrimeScript® reverse transcriptase kit (Takara, Japan). Gene expression was normalized to the expression level of β-actin ...
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bioRxiv - Microbiology 2021Quote: ... The entire fragment was then infused into a pCAGGS expression vector using an Infusion HD cloning kit (Takara Bio), according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... ssDNA was purified using the Nucleospin Gel and PCR Clean-up kit (Machery-Nagel, distributed by Takara Bio USA) with buffer NTC used as recommended by the manufacturer ...
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bioRxiv - Evolutionary Biology 2021Quote: The 5’ and 3’ ends of Cpun_dsx were amplified using the SMARTer RACE 5’/3’kit (TaKaRa, Shiga, Japan) and gene-specific primers designed for OD2 (“Cpun_dsx OD2 5’RACE” and “Cpun_dsx OD2 3’RACE” ...
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bioRxiv - Evolutionary Biology 2020Quote: ... while those with <15ng were prepared using the SMARTer® ThruPLEX® DNA-Seq Kit (Takara Bio Inc, Japan) in the sensi-lab at NHM Oslo (Table S1) ...
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bioRxiv - Developmental Biology 2020Quote: ... Complementary DNA libraries were prepared using the SMART-Seq v4 Ultra Low Input RNA kit for Sequencing (TAKARA Bio) and Nextera XT DNA Library Prep kit (Illumina) ...
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bioRxiv - Neuroscience 2022Quote: ... a sequence region of 5,000 bp between SnaBI and AgeI restriction sites was amplified using the HiFi amplification kit (Clontech, Takara Bio Europe ...
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bioRxiv - Molecular Biology 2022Quote: ... Equal volume RNAs were subjected to reverse transcription using the PrimeScript RT Reagent Kit with gDNA Eraser (Takara, Japan). Distribution of mat mRNAs or mat-exon1 mRNAs in transfected cells was assessed using SBYR-Green (Takara ...
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bioRxiv - Plant Biology 2022Quote: ... cDNA was synthesized from 500 ng total RNA using a Prime Script RT Reagent Kit (Takara Bio, Otsu, Japan). qRT-PCR was performed using a StepOne Real-time PCR Instrument (Applied Biosystems ...
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bioRxiv - Physiology 2022Quote: ... Messenger RNAs were reverse transcribed and amplified using the SMART-Seq V4 ultra low input RNA kit (Clontech, France) according to the manufacturer recommendations ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 g RNA was used to synthesize the cDNA using reverse transcription M-MLV (RNase-free) kits (TaKaRa, Japan). qRT-PCR was performed with SYBR green PCR master mix (ToYoBo ...