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Citations for Takara Bio :
2851 - 2900 of 4901 citations for Uridine Triphosphate UTP CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2021Quote: ... according to the technical manual for PrimeScriptTM First Strand cDNA Synthesis Kit (Takara Bio Inc., Mountain View, CA). Amplification of VH and VL antibody fragments was carried out according to the standard operating procedure (GenScript ...
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bioRxiv - Synthetic Biology 2021Quote: ... The cDNA was synthesized from 1 μg of total RNA using the PrimeScript™ RT-PCR Kit (Takara). Three technical replicates were measured for gene expression levels in each cDNA sample ...
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bioRxiv - Biophysics 2022Quote: ... Unique clones for each COP were sub-cloned in pRH2.2 using the In-Fusion Cloning kit (Takara Bio). Sequence-verified COPs were transformed into E ...
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bioRxiv - Biophysics 2022Quote: ... Resulting fragments were cloned into linearized FM5-mCh or FM5-GFP constructs using In-Fusion Cloning kit (Takara). The resulting plasmids were sequenced to confirm correct insertion.
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bioRxiv - Bioengineering 2021Quote: ... The virus titre was calculated using the ELISA-based Lenti-X™ p24 Rapid Titer Kit (Takara Bio) following the manufacturer’s instructions.
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bioRxiv - Neuroscience 2020Quote: ... #AM9937).The Brp-T2A-QF2w-Hsp70-3XP3-dsRed donor plasmid was constructed using the InFusion HD Kit (Clontech, #638910). Homology arms of ∼1 kb flanking the sgRNA cut site were cloned by PCR using template genomic DNA from the Orlando strain ...
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bioRxiv - Molecular Biology 2021Quote: ... Three μg of total RNA was subjected to reverse transcription using PrimeScript 1st strand cDNA Synthesis Kit (Takara).
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bioRxiv - Cancer Biology 2020Quote: ... Library construction was performed using the SMARTer® Stranded Total RNA-Seq Kit v2 – Pico Input Mammalian (Takara), and paired-end sequencing was performed on the NovaSeq 6000 instrument (Illumina ...
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bioRxiv - Neuroscience 2020Quote: ... Plasmid DNA to generate viral vectors was prepared using the NucleoBond Xtra Maxi endotoxin free kit (Takara Bio) prior to production ...
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bioRxiv - Neuroscience 2020Quote: cDNA libraries were made by the OHSU Massively Parallel Sequencing Shared Resource using the SMARTer RNA kit (Clontech). Library quality was assessed using a TapeStation 220 and libraries were quantified by qPCR using a KAPA Library Quantification kit ...
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bioRxiv - Plant Biology 2021Quote: ... Total RNA was extracted using the Mini BEST Plant RNA Extraction Kit (Takara Biotech Co., Ltd., Dalian, China) and cDNA was synthesized using the one-step PrimeScript RT-PCR Kit (Takara ...
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bioRxiv - Cell Biology 2021Quote: ... Protein concentrations were determined via the Bradford method using the TaKaRa Bradford Protein Assay Kit (Takara Bio, T9310A).
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bioRxiv - Plant Biology 2020Quote: ... using the HindIII and XbaI sites with the In-Fusion HD Cloning Kit (Takara Bio, Otsu, Shiga, Japan). The construct was verified by sequencing and used to transform Ler by the floral dip method (Clough and Bent ...
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bioRxiv - Plant Biology 2021Quote: ... and was reverse transcribed by the PrimeScriptTM RT reagent Kit with gDNA Eraser following the manufacturer’s instructions (TaKaRa). RT-qPCR was performed with SYBR Green mix (Bimake) ...
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bioRxiv - Microbiology 2020Quote: ... Pseudovirus-containing supernatants were collected 48 h later and concentrated using Lenti-X™ concentrator kit (Takara, CA). Pseudovirus neutralization assay was then performed by incubation of SARS-CoV-2 pseudovirus with 5 folds serially diluted antibody with starting concentration of 1 μg/ml for 1 h at 37 °C ...
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bioRxiv - Neuroscience 2020Quote: ... reverse transcription and amplification was achieved using the SMART-Seq v4 ultra low input RNA Kit (Takara, 634891). This kit improves synthesis of the full-length cDNA via a template switching mechanism for synthesis of the second strand cDNA ...
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bioRxiv - Neuroscience 2020Quote: ... cDNA library preparations were conducted using the SMART-Seq v4 Ultra Low Input kit from TaKaRa (Cat. # 634894) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... RNA was amplified using SMARTer® Universal Low Input RNA Kit for sequencing (Takara, Mountain View, CA, USA). Ribosomal RNA was depleted using RIboGone™-Mammalian rRNA removal kit (Takara ...
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bioRxiv - Molecular Biology 2021Quote: ... The respective PCR products were then cloned into pcDNA5_FRT_TO_3xFlag(N) using In-Fusion® HD Cloning Kit (Cat. No. 639650, Takara).
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bioRxiv - Microbiology 2021Quote: ... Reverse transcription was done using PrimeScript™ RT reagent Kit with gDNA Eraser (Perfect Real Time) (Takara, #RR047A). qPCR reaction was prepared by TB Green® Premix Ex Taq™ (Tli RNaseH Plus ...
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bioRxiv - Systems Biology 2021Quote: ... Ribosomal RNA was depleted using the RiboGone – Mammalian Kit protocol (Cat #634847, Takara Bio USA, Mountain View, CA). The SMARTer Universal Low Input RNA Kit protocol v2 (Cat #634938 ...
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bioRxiv - Microbiology 2020Quote: ... converted to single stranded cDNA and amplified using the SMARTer PCR cDNA Synthesis Kit (Takara Bio, Kusatsu, Japan), and IsoSeq libraries were constructed with equimolar cDNA fractions (0.5X and 1X ...
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bioRxiv - Cell Biology 2021Quote: Total RNA was isolated from wild-type or transiently transfected cells with MiniBEST Universal RNA Extraction Kit (Takara), an additional DNase I (NEB ...
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bioRxiv - Cell Biology 2020Quote: ... 100 ng of total RNA was reverse-transcribed using the PrimeScript RT Reagent Kit (Takara Bio, Shiga, Japan) and a T100 thermal cycler (Bio-Rad Laboratories ...
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bioRxiv - Cell Biology 2021Quote: ... total RNA was extracted using TRIzol reagent (Fermentas) and reverse transcribed using the mRNA Selective PCR Kit (TaKaRa) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: RNA was quantified (by Nanodrop) and retrotranscribed to cDNA (PrimeScript™ RT-PCR Kit - Takara Bio Cat. # RR014A). Real time PCR was performed with KAPA SYBR® FAST qPCR Master Mix (2X ...
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bioRxiv - Cell Biology 2021Quote: Total genomic DNA of HeLa cells was extracted after irradiation using Total genomic DNA extract kit (TAKARA, China). Human nuclear GAPDH gene (forward ...
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bioRxiv - Biochemistry 2022Quote: ... AAV-GFP and AAV-Mafa were purified with the AAV pro Extraction Solution Kit (6235, Takara, Shiga, Japan), and the titers were determined with a Quick Titer AAV Quantification Kit (VPK-145 ...
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bioRxiv - Physiology 2022Quote: ... cDNA was synthesized using 2 μg RNA using the PrimeScript™ RT reagent Kit with gDNA Eraser (TaKaRa). Gene specific primers for quantitative real-time PCR (qRT-PCR ...
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bioRxiv - Plant Biology 2022Quote: ... and 3’ UTR region was cloned into the PacI site in pZH_OsU6sgRNA_SpCas-wt (Endo et al., 2019) using an In-Fusion HD cloning kit (Takara). Two pairs of 20-nt annealed oligonucleotides targeting exon 4 of the OsSOG1 gene were cloned into the BbsI site of the pU6gRNA-oligo (pU6::sgOsSOG1-2 and pU6::sgOsSOG1-3 ...
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bioRxiv - Microbiology 2022Quote: ... a one-step reverse transcription-PCR (RT-PCR) assay was conducted with a PrimeScript kit (Takara, Tokyo, Japan), and Viral genomic terminal sequences were determined using commercial 5’ and 3’ RACE (rapid amplification of cDNA ends ...
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bioRxiv - Molecular Biology 2022Quote: ... The resulting cDNA was subjected to relative quantitative PCR using a SYBR Premix Ex Taq™ kit (TaKaRa) on a Roche LightCycler 480 real-time PCR machine ...
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bioRxiv - Biophysics 2022Quote: ... 3’-RACE-Ready cDNA template was synthesized using a SMARTer® RACE 5’/3’ Kit (Takara Bio, USA) and subsequently used to amplify 3’ end sequences of R ...
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bioRxiv - Cell Biology 2022Quote: RNA was extracted at each time point using the Nucleospin RNA XS kit from Clontech (cat. num. 740902.250). RNA quality was assessed using a NanoDrop and then Bioanalyzer was used to quantify RNA amount ...
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bioRxiv - Cell Biology 2022Quote: ... and inserted between BamHI and EcoRI restriction sites by using restriction enzymes and a DNA ligation kit (TaKaRa). Constructs of hCAP-H2 harboring charge-reversal mutations in the BC1 and BC2 regions (H2-BC1D ...
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bioRxiv - Plant Biology 2021Quote: ... First-strand cDNA was synthesized from 500 ng total RNA using the PrimeScript RT reagent kit (Takara, Japan). Quantitative RT-PCR (qRT-PCR ...
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bioRxiv - Neuroscience 2020Quote: ... For FANS RNA-seq library preparation (SMARTer Stranded Total RNA-Seq Kit Pico Input Mammalian, Clontech Laboratories, 635005), 2952pg of total nuclear RNA were amplified into cDNA with fragmentation times of 2.5 min for autopsy and 3.5 min for TLE cases (14 cycles total amplification for all) ...
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bioRxiv - Bioengineering 2021Quote: ... The PCR amplified CLIPtag were then ligated with the digested vectors using In-Fusion HD Cloning kit (Clontech). The plasmids pSNAPf-Clathrin-15 and pSNAPf-LMNA were directly purchased from UCSF Nikon Imaging Center ...
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bioRxiv - Immunology 2020Quote: ... q-RT-PCR was performed using the One Step PrimeScript™ RT-PCR Kit (Perfect Real Time; TaKaRa). The primers used for q-RT-PCR were selected to be specific for the E and ORF1ab sequences in the SARS-CoV-2 genome (Table 1) ...
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bioRxiv - Plant Biology 2021Quote: ... Isolated RNA was used as template for cDNA synthesis using PrimeScript RT Reagent Kit (Takara Bio, Kusatsu, Japan). Transcript expression was quantified by qRT-PCR using iQ SYBR Green supermix (Bio-Rad Laboratories ...
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bioRxiv - Molecular Biology 2020Quote: ... The purified mixture of PCR products was cloned into pMD20-T vector using Mighty TA-cloning Kit (TaKaRa). The 119 clones for AAVS-tid GTC_70-107 (+ ...
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bioRxiv - Neuroscience 2020Quote: ... Genomic titer was determined using the Lenti-Z qRT-PCR Titration Kit according to manufacturer’s instructions (Takara #631235). Smaller scale virus prep for sgRNAs and shRNAs was performed through a similar transfection in a 12-well culture plate ...
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bioRxiv - Immunology 2020Quote: ... mRNA library preparation was performed following manufacturer’s recommendations (SMART-Seq v4 Ultra Low Input RNA Kit, TAKARA Bio). Final samples pooled library prep was sequenced on Nextseq 500 ILLUMINA with HighOutPut cartridge (2×400Millions of 75 bases reads) ...
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bioRxiv - Microbiology 2021Quote: DNA was extracted from 250 mg of sponge sample using the Nucleospin Soil DNA extraction kit (Takara Bio) and was submitted to the Baylor College of Medicine’s Alkek Center for Metagenomics & Microbiome Research for high-throughput sequencing ...
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bioRxiv - Plant Biology 2021Quote: ... The PCR products were cloned into the modified pET28 vector using the In-Fusion HD Cloning Kit (Clontech) to generate pET28M-MpaKAI2A and pET28M-MpaKAI2B ...
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bioRxiv - Plant Biology 2021Quote: ... cDNA synthesis was performed with 1 μg of total RNA using the PrimeScript™ RT Reagent Kit (Takara) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... poly-A libraries were prepared using the SMARTer ThruPLEX DNA-seq Kit (R400675, Takara, Mountain View, CA, USA). Each library was normalized to 1.5 nM ...
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bioRxiv - Cell Biology 2022Quote: ... Titer of the lentiviruses was measured with a lentivirus titration kit (Takara Bio USA, San Jose, CA, USA). The same multiplicity of infection (MOI ...
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bioRxiv - Molecular Biology 2022Quote: ... cDNA libraries were prepared with the SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian (Takara, 634413). All samples were sequenced on Illumina NextSeq500 (Illumina).
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bioRxiv - Developmental Biology 2022Quote: ... 500 ng of total RNA was reverse-transcribed into cDNAs using the PrimeScript RT Reagent Kit (TaKaRa, RR037A) according to the manufacturer’s protocol ...