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Citations for Takara Bio :
3301 - 3350 of 4901 citations for Uridine Triphosphate UTP CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... Total RNA was isolated from cells or tumors using a Mini BEST Universal RNA Extraction Kit (Takara, Shiga, Japan). cDNA was prepared from total RNA by reverse transcription using oligo-dT primers (Takara) ...
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bioRxiv - Immunology 2024Quote: ... All ChIP-seq and input DNA libraries were made using ThruPLEX DNA-Seq kit (cat #R400674, Takara Bio, USA) following the manufacturer’s instructions ...
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bioRxiv - Genetics 2024Quote: ... Approximately 0.3 mg of total RNA was converted into cDNA with the PrimeScript RT Reagent Kit (Takara, Japan, RR037A) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... The isolated RNA was RNase III fragmented and adaptor-ligated using PrepX mRNA Library kit (Takara, Mountain View CA), then converted into cDNA using Superscript III reverse transcriptase (Lifetech ...
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bioRxiv - Microbiology 2024Quote: ... cDNA was synthesized from 1 µg of each RNA sample using PrimeScript RT Reagent Kit with gDNA Eraser (Takara), and the primers used for qRT-PCR were designed using Primer3web ...
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bioRxiv - Cancer Biology 2024Quote: ... Barcode sequence of each clone was PCR amplified directly from the cells using Terra PCR Direct Polymerase kit (Takara) for Sanger sequencing using previously described primers (5).
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bioRxiv - Bioengineering 2024Quote: ... [50] The ssDNA scaffold was initially prepared using Guide-it Long ssDNA Production System v2 kit (Takara Bio, 632666) following manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... and the remaining RNA isolation was done using the NucleoSpin RNA II Kit (Clontech Laboratories, Palo Alto, CA; 740955.50). 1 μg RNA was transferred into the cDNA Ecodry Premix Kit prior to the quantitative PCR program being run on ABI QuantStudio 3 with iTaq Universal SYBR Green Supermix (Bio-Rad ...
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bioRxiv - Bioengineering 2024Quote: ... and reverse-transcribed into cDNA using PrimeScript™ RT reagent Kit with gDNA Eraser (Perfect Real Time) (Takara Bio). The resulting cDNA was utilized for analysis using TB Green® Premix Ex Taq™ II (Tli RNaseH Plus ...
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bioRxiv - Biochemistry 2024Quote: ... This was followed by ligation of the guide RNA to the ligation oligo using the DNA Ligation Kit (Takara). Subsequently ...
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bioRxiv - Cancer Biology 2024Quote: ... The cDNA synthesis and gDNA erase protocol were performed using the PrimeScript RT reagent Kit with gDNA Eraser (Takara). Quantitative real-time RT-PCR (qRT-PCR ...
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bioRxiv - Cancer Biology 2024Quote: ... Reverse-transcription-quantitative PCR (RT-qPCR) was conducted using the Mir-X miRNA qRT-PCR TB Green Kit (TAKARA), the reverse-transcription samples ...
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bioRxiv - Biophysics 2024Quote: Lentivirus titration was determined by p24 ELISA using Lenti-X™ p24 Rapid Titer Kit (Takara Bio, no. 632200) and a plate reader (PerkinElmer Victor3 V) ...
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bioRxiv - Cell Biology 2024Quote: ... Reverse transcription of RNA into cDNA was accomplished with the PrimeScript RT reagent Kit with gDNA Eraser (RR047A, Takara), followed by quantitative RT-PCR (qRT–qPCR ...
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bioRxiv - Genetics 2024Quote: ... cells were harvested and vectors were purified using the AAVpro purification kit (cat.: 6666; Takara Bio, Kusatsu, Shiga, Japan) as per manufacturer’s instructions and stored at -80 °C until further use ...
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bioRxiv - Genetics 2023Quote: ... RNA amplification was performed using the SMART-Seq® v4 Ultra® Low Input RNA Kit for Sequencing (TaKaRa) and cDNA libraries were prepared using Nextera XT DNA Sample Preparation Kit (Illumina) ...
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bioRxiv - Cell Biology 2022Quote: ... Reverse transcription was performed with 10 ng RNA using the SMART-Seq v4 Ultra Low Input RNA Kit (Takara) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... and 1 ug of RNA was reverse-transcribed into cDNAs using the Prime Script RT Reagent Kit (Takara, RR037B) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: Samples were then processed using the SMART-seq Stranded kit (Cat. 634444, protocol version 051018; Takara Bio USA, Inc.) according to the manufacturer’s protocol with minor modifications ...
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bioRxiv - Plant Biology 2023Quote: ... The gRNA was then ligated into BsaI linearized pKSE401 using DNA ligation Kit v.2.0 (TaKaRa, Kusatsu, Shiga, Japan).
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bioRxiv - Biophysics 2022Quote: ... Unique clones were sub-cloned in pRH2.2 (a gift of S. Sidhu) using the In-Fusion Cloning kit (Takara) and sequence-verified ...
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bioRxiv - Cell Biology 2023Quote: ... 2xmCh-KIF5C in pcDNA3.1 was cloned by inserting in-frame KIF5C full length from EGFP-KIF5C (a gift from Anthony Brown) 2xmCh sequence from KIF5C(1-560)-2xmCh-EF(C) into pcDNA3.1 using InFusion Cloning kit (Takara). EGFP-Rab7A (Addgene #28047 ...
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bioRxiv - Molecular Biology 2023Quote: ... or into pHTN-HaloTag vector for GID4-HaloTag N-terminal fusion using the In-Fusion HD Cloning kit (Takara). Pro/N-degron coding sequence was cloned into pNLF1-C for MPGLWKS-NanoLuc C-terminal fusion ...
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bioRxiv - Molecular Biology 2023Quote: ... ChIP-seq libraries were prepared with sheared DNA (0.5 ng of ChIP DNA or 5.0 ng of input DNA) using ThruPLEX® DNA-seq Kit (Clontech). These obtained libraries were size-selected at 300-700 bp using ×1.0 AMPure XP beads (Beckman Coulter) ...
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bioRxiv - Microbiology 2022Quote: ... using the primers listed in S-Table 1B into pcDNA3.1-P2A-tagRFP by the In-Fusion HD Cloning Kit (Takara), according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... RT-qPCR detection was performed on each group of samples by using a reverse transcription kit (Takara Bio, Japan) and fluorescence quantitative kit (Takara Bio ...
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bioRxiv - Neuroscience 2023Quote: TUNEL assays were performed to detect apoptotic cell death using Clonetech ApoAlert DNA Fragmentation kit (Takara, Kusatsu, Shiga, Japan) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: cDNA was amplified from the collected cells using the SMART-Seq v4 Ultra Low Input RNA Kit (Clontech-TakaraBio). 1ng of amplified cDNA was used to generate barcoded libraries with the Nextera XT DNA library preparation kit (Illumina) ...
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bioRxiv - Cell Biology 2023Quote: ... Full length cDNAs were generated from 350 to 1,000 pg of total RNA using Clontech SMART-Seq v4 Ultra Low Input RNA kit for Sequencing (Takara Bio Europe ...
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bioRxiv - Neuroscience 2023Quote: ... mRNA was transcribed into full-length complementary DNA using a SMART-Seq v4 Ultra Low Input RNA kit (Clontech) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... ssRNA was converted into cDNA using SMARTer Universal Low Input RNA Kit according to the manufacturer’s protocol (Takara Bio). After PCR amplification ...
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bioRxiv - Microbiology 2023Quote: ... The p24 amount in the medium was quantified by Lenti-X p24 Rapid Titer Kit (632200, Takara Bio Inc.) with the p24 control samples ...
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bioRxiv - Genomics 2023Quote: ... The digested and purified reporter pool was then ligated into 80 ng of the linearised entry vector using Takara ligation kit v1.0 (#6021; Takara) at a 1:3 (vector:insert ...
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bioRxiv - Synthetic Biology 2023Quote: ... Cells were then lifted using TrypLE and total RNA was extracted using the Takara RNA plus kit following manufacturer’s instructions (Takara). 5 µL of total mRNA was converted to total cDNA using random hexamers following manufacturer’s instructions (Verso ...
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bioRxiv - Bioengineering 2023Quote: ... following which these sequences were inserted into the parental plasmid using In-Fusion® cloning kit (Takara Bio Inc.).
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bioRxiv - Cancer Biology 2023Quote: ... and used to produce barcoded cDNA sequencing libraries using the SMARTer® Universal Low Input RNA Kit (Takara, 634938). Libraries were sequenced on Illumina NovaSeq S4 (paired-end 2×150 ...
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bioRxiv - Cancer Biology 2023Quote: ... and used to produce barcoded cDNA sequencing libraries using the SMARTer® Universal Low Input RNA Kit (Takara, 634938). Validation of TT-TL procedure was performed by qPCR (TableS7 for primer sequences) ...
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bioRxiv - Bioengineering 2023Quote: ... The linearized pET28a vector and mfl fragment were ligated using the In-Fusion HD Cloning Kit (Clontech, Takara, Japan), and the ligated product was then transformed into E ...
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bioRxiv - Bioengineering 2023Quote: ... The linearized pET28a vector and mfl fragment were ligated using the In-Fusion HD Cloning Kit (Clontech, Takara, Japan), and the ligated product was then transformed into E ...
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bioRxiv - Microbiology 2023Quote: ... and an aliquot of 100 ng was reverse transcribed with the PrimeScript RT Reagent Kit with gDNA Eraser (Takara) and the included primer mix ...
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bioRxiv - Cell Biology 2022Quote: ... Total RNAs from the H1 and H2 hybridomas were purified using the NucleoSpin RNA purification kit (TAKARA, Tokyo, Japan) as described in the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... Full-length cDNAs were generated directly from ~10,000 cells per sample using oligo(dT) priming and the SMART-Seq v4 Ultra Low Input RNA Kit (Clontech), and 150 pg of cDNA was used to prepare strand-specific paired-end sequencing libraries (Nextera XT ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The product from this PCR was purified and cleaned with NucleoSpin Gel and PCR clean-up kit from TAKARA. A 2nd PCR was performed with high-fidelity PrimeSTAR GXL DNA Polymerase (Takara Cat ...
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bioRxiv - Biochemistry 2022Quote: ... total RNA (1.9 mg) was used to synthesize first-strand cDNA using the Mir-X miRNA First-Strand Synthesis Kit (TaKaRa) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... was generated from a Katnal1 conditional KO targeting construct using the Guide-it Long ssDNA Strandase Kit (Takara, 632645). The following oligonucleotides were used to generate a PCR product from which the ssDNA was generated ...
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bioRxiv - Microbiology 2022Quote: ... Isolated RNA or serial ten-fold dilutions of RNA standards for the ORF1ab amplicon (ranging from 2.25×10^6 to 250 copies/rxn) were reverse transcribed using the Takara Prime Script RT kit (Takara) using poly(A ...
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bioRxiv - Developmental Biology 2023Quote: ... Start codon mutant constructs were generated by mutation PCR using the PrimeSTAR Mutagenesis Basal Kit (Takara Bio, Kusatsu, Japan) by replacing AUG with AAG at Met1 ...
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bioRxiv - Cancer Biology 2023Quote: ... cDNA amplification was performed using the SMART-Seq® v4 Ultra® low input RNA kit (Takara, cat. # 634891) with sixteen cycles of PCR ...
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bioRxiv - Developmental Biology 2023Quote: ... and the viral packing vectors (pCMV-ΔR8.9 and pCMV-VSV-G) using the CalPhos mammalian transfection kit (631312, Clontech). pCW57.1-Luciferase and pCW57.1-mDux-CA were a gift from Stephen Tapscott (Addgene plasmids #99283 and #99284) ...
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bioRxiv - Molecular Biology 2023Quote: ... and then used as the template to synthesize double strand cDNA amplicons by PCR (optimal 17 – 21 cycles) using Advantage 2 PCR kit according to the manufacturer’s specifications (Clontech). cDNA was purified using NucleoSpin Gel and PCR Clean-up kit (Takara Bio) ...