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Citations for Takara Bio :
2651 - 2700 of 4900 citations for mu p75 SAP Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... ds-cDNA and was prepared using the SMARTer Ultra Low RNA kit for Illumina Sequencing (Takara-Clontech) per manufacturer’s protocol ...
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bioRxiv - Biochemistry 2021Quote: ... and total RNA was reverse-transcribed into cDNA by a reverse transcription kit (Takara Bio Inc., Japan). Then ...
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bioRxiv - Cell Biology 2020Quote: ... SMART-Seq V4 Ultra Low RNA Input Kit for Sequencing (Takara Bio USA, Inc., Mountain View, CA) was used for reverse transcription and generation of double stranded cDNA for subsequent library preparation using the Nextera XT Library Preparation kit (Illumina ...
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bioRxiv - Epidemiology 2021Quote: ... and SYBR Premix Ex Taq Kit for real-time PCR assay were purchased from Takara (Dalian, China). Monoclonal CD3-FITC/APC ...
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bioRxiv - Physiology 2021Quote: ... Adenovirus was generated in HEK293A cells and titered using the Adeno-X Rapid Titer Kit (Takara Bio). For differentiation ...
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bioRxiv - Developmental Biology 2020Quote: ... DNase treated RNA was used for cDNA synthesis through PrimeScript II 1st Strand cDNA Synthesis Kit (Takara) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... from Mnemiopsis embryos collected at different developmental stages and used to generate cDNA libraries (SMARTer kit, Clontech). The coding sequences of MleKlf5a ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and the RNA-seq libraries were constructed using SMARTer Stranded Total Pico-Input RNA-seq kit (Clontech) and 10 ng RNA from each sample ...
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bioRxiv - Genomics 2020Quote: cDNA was synthesized from parasite RNA using the SMARTer PCR cDNA synthesis kit (Takara, Mountain View, CA), which incorporates a poly(A ...
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bioRxiv - Microbiology 2020Quote: ... Cellular RNA was isolated at 6 hpi using the CellAmp Direct RNA Prep Kit (3732; Takara, Japan). The RNA was then diluted in water and boiled ...
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bioRxiv - Microbiology 2020Quote: cDNAs were synthesized by reverse transcription using a PrimeScript II 1st Strand cDNA Synthesis Kit (Takara Bio). Total RNA (1 μg ...
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bioRxiv - Microbiology 2020Quote: ... and was cloned subsequently into pCAGGS vector using In-Fusion® HD Cloning Kit (Takara Bio USA) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... The baculovirus master and working stock titers were determined using rapid titer kit (Clontech, Mountain View, CA). Recombinant baculovirus stocks were prepared by infectingSf9 cells with a multiplicity of infection (MOI ...
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bioRxiv - Immunology 2020Quote: ... for RPE samples or the SMART-Seq HT Ultra Low Input RNA Kit (Takara Bio USA, Inc.) for MΦ/μglia samples ...
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bioRxiv - Molecular Biology 2020Quote: ... The cDNA was synthesized from the treated RNA using Primer Script RT kit (Takara Bio, Dalian, China), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... followed by cDNA synthesis using a PrimeScript RT Reagent Kit with gDNA Eraser (Takara-Bio, Kusatsu, JAPAN). RT-qPCR using Brilliant II SYBR® Green QPCR Master Mix (Agilent ...
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bioRxiv - Immunology 2020Quote: ... the DNA-amplicons for Illumina sequencing were generated by using Advantage 2 PCR Kit (TAKARA Bio Europe), employing a mTCRβ reverse primer and a Universal primer mix (UPM) ...
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bioRxiv - Microbiology 2021Quote: ... and the in vitro Transcription T7 kit (for siRNA synthesis; No. 6140, TaKaRa Bio Inc., Kusatsu, Japan) was used ...
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bioRxiv - Immunology 2021Quote: The RNA was converted to cDNA with the Clontech SMARTer kit (Takara Bio USA; Mountain View, CA) using the modified (Switching Mechanism At 5’ End of RNA Transcript ...
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bioRxiv - Molecular Biology 2020Quote: ... The first strand cDNA was synthesized for use with the PrimeScript Reverse Transcriptase kit (TaKaRa, Dalian, China) according to the instructions of the manufacturer ...
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bioRxiv - Microbiology 2021Quote: ... The loss of HIF1α was further confirmed by sequencing using the Guide-it Indel kit (Takara #631444) and primers targeting the corresponding genomic regions ...
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bioRxiv - Immunology 2022Quote: ... and cDNAs were synthesized using a SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Clontech) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... gDNA from all cells were isolated using the Machery Nagel L Midi NucleoSpin Blood Kit (Clontech, 740954.20). Modifications to the manufacturer’s instructions were added as follows ...
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bioRxiv - Evolutionary Biology 2022Quote: ... with 0.1 µL of Taq Polymerase 5 U.µL-1 (TaKaRa Ex Taq® Kit MgCl2 Free Buffer) (TaKaRa Ex Taq ...
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bioRxiv - Neuroscience 2022Quote: ... and then cloned into pGEMHE using In-Fusion HD Cloning Kit according to the manufacturer’s instructions (Clontech).
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bioRxiv - Cell Biology 2022Quote: ... and mRNA reverse transcription was performed with the PrimeScript RT Reagent Kit with gDNA Eraser (Takara, Japan). The qPCR was performed using SYBR Green qPCR Master Mix (Takara ...
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bioRxiv - Genomics 2022Quote: ... and first-strand cDNA was synthesized using a PrimeScript RT Reagent Kit (Catalog No. RR037A, TaKaRa, Tokyo), which was used for subsequent transcriptomic analyses ...
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bioRxiv - Immunology 2022Quote: ... qRT-PCR was performed using the SYBRGreen included in the BacPAK™ qPCR titration kit (Clontech, USA) and a StepOnePlus™ Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Genetics 2022Quote: ... Reverse transcription for cDNA cloning was performed with PrimeScript™ II 1st Strand cDNA Synthesis kit (Takara), Gibson assembly was performed with NEBuilder HiFi DNA Assembly Master Mix ...
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bioRxiv - Plant Biology 2022Quote: ... Total RNA was used for cDNA synthesis (PrimeScript RT Reagent Kit; Takara Bio, Takara Bio, Kusatsu, Japan). Transcript expression was quantified by real-time qPCR using iQ SYBR Green supermix (Bio-Rad Laboratories ...
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bioRxiv - Plant Biology 2022Quote: ... Total RNA was used for cDNA synthesis (PrimeScript RT Reagent Kit; Takara Bio, Takara Bio, Kusatsu, Japan). Transcript expression was quantified by real-time qPCR using iQ SYBR Green supermix (Bio-Rad Laboratories ...
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bioRxiv - Plant Biology 2022Quote: ... cDNA was synthesized from total RNA using PrimeScript™ reverse transcriptase reagent Kit (Perfect Real Time) (Takara) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... PCR products were purified using NucleoSpin Gel and PCR Clean-Up kit (Takara Bio, Kusatsu, Shiga, Japan), and 600 ng-1 ug of DNA templates were added to set up in vitro transcription reactions ...
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bioRxiv - Molecular Biology 2022Quote: ... The resulting cDNA was subjected to relative quantitative PCR using the SYBR Premix Ex Taq kit (TaKaRa) on a Roche LightCycler 480 real-time PCR machine ...
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bioRxiv - Microbiology 2022Quote: ... Viral titer was determined by the ELISA p24 antigen assay (Lenti-X p24 Rapid Titer Kit, TaKaRa). Infections of fully differentiated MDM were performed after adherent growth in the presence of M-CSF for seven days ...
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A modular CRISPR screen identifies individual and combination pathways contributing to HIV-1 latencybioRxiv - Microbiology 2022Quote: ... and used for plasmid DNA preps using the Endotoxin-Free Nucleobond Plasmid Midiprep kit (Takara Bio; 740422.10). The HuEpi library was sequenced and contains all 5,309 sgRNAs included in the synthesis (GSE215430).
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bioRxiv - Molecular Biology 2022Quote: ... The in vitro assembly of PCR products was performed by using In-Fusion HD cloning kit (Takara). The assembled plasmids were amplified by transformation of Stellar Competent Cells (Takara ...
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bioRxiv - Neuroscience 2023Quote: Neurons were transfected at day in vitro (DIV) 7 using CalPhos Mammalian Transfection Kit (Takara Bio, 631312). Transfection solution was prepared by adding 1.5 µg DNA per construct (3 µg in total ...
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bioRxiv - Plant Biology 2023Quote: ... Poly-A tail enriched cDNA libraries were prepared using the SMART-Seq HT Kit (Takara Bio, Japan), quality checked using the TapeStation DNA ScreenTape (Agilent Technologies ...
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bioRxiv - Plant Biology 2023Quote: ... Total RNA was used for cDNA synthesis (PrimeScript RT Reagent Kit; Takara Bio, Takara Bio, Kusatsu, Japan). Transcript expression was quantified by real-time qPCR using iQ SYBR Green super mix (Bio-Rad Laboratories ...
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bioRxiv - Plant Biology 2023Quote: ... Total RNA was used for cDNA synthesis (PrimeScript RT Reagent Kit; Takara Bio, Takara Bio, Kusatsu, Japan). Transcript expression was quantified by real-time qPCR using iQ SYBR Green super mix (Bio-Rad Laboratories ...
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bioRxiv - Microbiology 2022Quote: ... the corresponding cDNA was transcribed using a Fast Quant RT Kit with gDNA (TaKaRa Bio, Shiga, Japan). The cDNA was used as a template for RT-PCR assays ...
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bioRxiv - Molecular Biology 2022Quote: ... the total RNA was reverse transcribed to cDNA using the Prime ScriptTM RT reagent kit (TaKaRa Biotechnology). qPCR was performed using the SYBR Premix Ex Taq II Kit (TaKaRa Biotechnology ...
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bioRxiv - Molecular Biology 2022Quote: ... The DNA band was cut out and purified using the Nucleospin® Extract II Kit (Clontech Laboratories) protocol ...
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bioRxiv - Plant Biology 2022Quote: ... The downstream genomic region was then amplified using the primer set MpHEC1_CDS_IF_F and MpHEC1_3UTR_IF_R2 and integrated into the first binary plasmid with an In-Fusion HD Cloning Kit (Clontech) using the BamHI and AscI sites ...
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bioRxiv - Microbiology 2022Quote: ... DNA fragments were assembled into pEMC vector one-by-one using in-fusion HD cloning kit (Takara), generating pEMC-eGFP-MBaMV ...
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bioRxiv - Microbiology 2022Quote: ... and used for plasmid DNA preps using the Endotoxin-Free Nucleobond Plasmid Midiprep kit (Takara Bio; 740422.10). The HIVDEP library was sequenced and contains all 4,191 sgRNAs included in the synthesis (GEO Dataset ...
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bioRxiv - Molecular Biology 2022Quote: ... rAAVs were purified using AAVpro® Purification Kit for All Serotypes (Cat # 6666, Takara Bio USA, CA) and each of the purified rAAVs were tested in separate transwell BBB cultures with hCMEC/D3 cells and primary astrocytes at MOI of 1e4 ...
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bioRxiv - Molecular Biology 2022Quote: cDNA was synthesized with 1 μg of total RNA using the Prime ScriptTM RT Reagent Kit (Takara) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... and ligated into the digested lentiCRISPRv2 backbone using the DNA ligation kit (Mighty Mix) (Takara, Cat# 6023). The ligated DNA was then transformed into One Shot Stabl3 competent cells (Thermo Fisher Scientific ...