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Citations for Takara Bio :
2901 - 2950 of 4900 citations for mu p75 SAP Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... Base substitutions were induced in the pMXs vector with the PrimeSTAR® Mutagenesis Basal Kit (TaKaRa Bio Inc.) before transfection into Plat-E cells to create ALK-TKIs resistant mutants.
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bioRxiv - Genetics 2022Quote: cDNA was generated from 1 μg of total RNA by using the PrimeScript RT Reagent Kit (Takara, Japan) in accordance with the manufacturer’s protocol as previously described [21] ...
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bioRxiv - Biochemistry 2022Quote: ... and the DNA was extracted and then DNA libraries were prepared with Smarter Thruplex DNAseq Kit (Clontech, R400674) following the manufacturer’s protocols with amplification for 10-13 circles ...
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bioRxiv - Microbiology 2022Quote: ... Release of LDH into the culture supernatant was quantified after infection using an LDH Cytotoxicity Detection Kit (Clontech). LDH release was normalized to mock-infected cells and cells treated with 1% Triton to establish maximum LDH release ...
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bioRxiv - Developmental Biology 2022Quote: Purified total RNA was reverse transcribed into cDNA by PrimeScript™ 2 1st strand cDNA Synthesis Kit (TaKaRa). The target gene fragments (Brachyury ...
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bioRxiv - Neuroscience 2019Quote: ... Total RNA from the tissue samples was extracted using a TaKaRa MiniBEST Universal RNA Extraction Kit (TaKaRa, Japan) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2019Quote: ... 2.4 µg of clean RNA was reverse-transcribed to cDNA using Takara RNA PCR kit (Takara, Kyoto, Japan). Gene-specific primers (Table S3 ...
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bioRxiv - Neuroscience 2019Quote: ... Single stranded cDNA from the total RNA was synthesized with a RT-PCR kit (Clontech, Mountain View, CA) according to the kit’s instructions ...
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bioRxiv - Bioengineering 2019Quote: ... and reverse-transcribed by a PrimeScript™ RT reagent Kit with gDNA Eraser (Perfect Real Time) (TAKARA, Japan). qPCR was performed with SYBR Premix Ex Taq™ II (Tli RNaseH Plus ...
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bioRxiv - Molecular Biology 2019Quote: ... Libraries were prepared with 50 ng starting material as instructed by the ThruPLEX DNA-seq kit (R400427, Takara). Amplification was performed with 5 rounds of PCR ...
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bioRxiv - Neuroscience 2019Quote: ... and cloned into pEX-A (Eurofins Genomics, USA) using an In-Fusion HD Cloning Kit (Takara Bio, Japan). The cloned plasmids were transformed into Stellar competent cells (Takara Bio ...
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bioRxiv - Physiology 2019Quote: ... cDNA synthesis and PCR amplification were performed using PrimeScript First-Strand cDNA Synthesis kit (TaKaRa Bio, Tokyo, Japan) with 1 μg total RNA plus random hexamers ...
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bioRxiv - Immunology 2019Quote: ... using the manufacturers protocol and amplified using SMARTer Ultra Low Input RNA kit for sequencing (version 4, Clontech). Sequencing libraries were generated using TruSeq Nano DNA Sample Preparation kits (Illumina) ...
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bioRxiv - Evolutionary Biology 2019Quote: ... and 1000 ng of total RNA were reverse transcribed using the PrimeScript RT-qPCR Kit (TaKaRa, Cat#RR037B). At least four independent biological replicates were collected and analyzed ...
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bioRxiv - Cancer Biology 2019Quote: ... Dox-inducible vectors were generated according to a publicly available protocol60 using In-Fusion HD Cloning Kit (Clontech) (Supplementary Table 5) ...
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bioRxiv - Molecular Biology 2019Quote: ... Then the mCherry cassette was replaced by Puro or EGFP by In-Fusion® HD Cloning Kit (Clontech). To generate the enCRISPRi-LK sgRNA vector ...
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bioRxiv - Plant Biology 2019Quote: ... Real-time quantitative RT-PCR was performed with a PrimeScript II 1st strand cDNA Synthesis Kit (TaKaRa Bio) and SYBR Premix Ex Taq II (TaKaRa Bio) ...
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bioRxiv - Microbiology 2019Quote: ... Equal volume RNA was reverse transcribed into cDNA using the PrimeScript RT Reagent Kit with gDNA Eraser (Takara) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... full-length cDNA synthesis from poly-A containing transcripts was performed using the Clontech SMARTer PCR kit (Takara). The resulting cDNA was PCR amplified to generate 1-2ug cDNA ...
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bioRxiv - Genomics 2019Quote: ... The cDNA for reverse-transcriptase PCR is prepared using PrimeScript™ first strand cDNA Synthesis Kit from TaKaRa. The cDNA is appropriately diluted to proceed for analysis by quantitative PCR
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bioRxiv - Cancer Biology 2020Quote: ... cDNA was first amplified by reverse transcription using the PrimeSCript RT reagent kit (Takara Bio, Inc, Otsu, Japan). Then the relative expressions were measured with TaqMan MicroRNA Assay kit (Applied Biosystems ...
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bioRxiv - Genetics 2021Quote: ... and cDNA libraries were constructed for RNA sequencing by using a SMART-Seq HT Kit (Takara, Shiga, Japan) and a Nextera XT DNA Library Preparation Kit (Illumina ...
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bioRxiv - Synthetic Biology 2020Quote: ... Plasmid constructions were performed with standard restriction enzyme cloning or In-fusion HD Cloning kit (Takara Bio USA) according to the manufacturer’s protocol ...
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bioRxiv - Pathology 2021Quote: ... polyA enriched RNAs were subjected to RNA-sequencing library preparation using the SMART-Seq v4 Kit (SSv4; Takara) and sequenced using a paired-end 150bp configuration with 30M supporting reads per sample ...
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bioRxiv - Microbiology 2020Quote: ... The pSMART BAC-T7-scv2-replicon-Amp DNA was prepared using NucleoBond BAC 100 kit (Takara Cat# 740579). A fragment containing the RdRp D760N D761N mutation sequence was amplified by PCR using primers 5’-CGTAAACATTTCTCAATGATGATACTCTCTAACAATGCTGTTGTGTGTTTCAATAG-3’ and 5’-CAAAGAACACAAGCCCCAACAGCCTGTAAGACTGTATGCGGTGTGTACATAGC-3’ ...
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Airway Gene-Expression Classifiers for Respiratory Syncytial Virus (RSV) Disease Severity in InfantsbioRxiv - Immunology 2020Quote: ... 1 ng of total RNA was amplified using the SMARter Ultra Low amplification kit (Clontech, Mountain View, CA) and libraries were constructed using the NexteraXT library kit (Illumina ...
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bioRxiv - Bioengineering 2021Quote: ... One microgram of RNA was reverse transcribed into cDNA using a PrimeScript™ RT reagent Kit (TaKaRa, RR037A) in a 20 μl reaction ...
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bioRxiv - Neuroscience 2019Quote: Amplified cDNA from RNA was prepared using a SMART-Seq v4 Ultra Low Input RNA Kit (Takara 634898) protocol as per the manufacturer’s guidelines ...
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bioRxiv - Neuroscience 2019Quote: ... RNA was divided in two equal samples and used for amplification with SMART RACE cDNA Amplification Kit (Clontech) according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... URA3: GAL1UAS–Gal1TATA–LacZ MEL1) via the Yeastmaker™ Yeast Transformation System 2 kit (Clontech, Mountain View, USA). An agarose gel image of the cDNA library is shown in Fig ...
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bioRxiv - Immunology 2020Quote: ... 0.7-1 ng total RNA with an integrity index from 8.3 to 9.9 was amplified using the SMART-Seq v4 Ultra Low Input RNA Kit (Clontech), with 12 cycles of amplification ...
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bioRxiv - Molecular Biology 2019Quote: ... One microgram of total RNA was reverse transcribed to cDNA using RevertAid First Strand cDNA Synthesis Kit (TaKaRa) and stem-loop primers ...
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bioRxiv - Cell Biology 2019Quote: ... Point mutants were generated using the In-Fusion HD cloning kit (TakaraBio/Clontech, Saint-Germain-en-Laye, France). DNA sequencing was carried out by LGC Genomics (Berlin ...
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bioRxiv - Molecular Biology 2019Quote: ... Circulating miRs were isolated from 300 µL serum by using a NucleoSpin microRNA isolation kit (TaKaRa, Otsu, Japan).
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bioRxiv - Genomics 2021Quote: Sequencing libraries were generated using SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian (Takara Bio, #634413). The library preparation protocol started from 6 μL eluate for the biofluid samples and 100 ng (or 10 ng ...
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bioRxiv - Neuroscience 2021Quote: ... Full length cDNAs were generated using Clontech SMART-Seq v4 Ultra Low Input RNA kit for Sequencing (Takara Bio Europe ...
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bioRxiv - Developmental Biology 2021Quote: ... cDNA synthesis for Next-Generation Sequencing (NGS) was performed by SMARTer Stranded RNA-seq kit (Clontech Laboratories, USA) as recommended by the manufacturer ...
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bioRxiv - Microbiology 2021Quote: ... Reverse transcription was performed using a PrimeScript™ RT Reagent Kit (Takara Bio, Mountain View, CA 94043 USA). qPCR was performed with gene specific primers and SYBR Green (iTaq Universal SYBR Green Supermix ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Both missing ends could be recovered by RACE PCR (SMARTer RACE kit, Takara Bio, Mountain View, CA, USA).
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bioRxiv - Microbiology 2021Quote: ... RNA was then reverse transcribed into cDNA by PrimeScript RT reagent Kit with gDNA Eraser (Takara Bio Inc.). Gene mRNA levels were determined using SYBR green dye on Applied Biosystems Q6 Real-Time PCR cycler (Thermo ...
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bioRxiv - Biochemistry 2021Quote: The mutant cDNAs of bovine rhodopsin (accession no. AB062417) were constructed using an In-Fusion cloning kit (Clontech). The wild-type and mutant cDNAs of bovine rhodopsin were inserted into the mammalian expression vector pUSRα 24 or pCAGGS 25 ...
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bioRxiv - Immunology 2022Quote: ... cDNA generation and amplification were performed with SMART-Seq v4 ultra Low Input RNA kit (Takara Bio 634891). Amplified cDNA was purified using AMPure XP Beads (Beckman Coulter A63881) ...
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bioRxiv - Plant Biology 2021Quote: ... one microgram of DNase-treated RNA was used for reverse transcription using a PrimerScript RT reagent kit (Takara) and a mix of oligo poli-dT and random hexamers ...
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bioRxiv - Evolutionary Biology 2021Quote: ... cDNAs were synthesized using the SMARTer RACE cDNA Amplification Kit (Cat. No. 634858; Takara, Mountain View, CA, USA). In silico predicted pou-iv gene sequence was retrieved from the Joint Genome Institute genome database (Nematostella vectensis v1.0 ...
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bioRxiv - Cancer Biology 2021Quote: ... lncRNA NEAT1 and MMP2 mRNA were reversed transcribed into cDNA using PrimeScript RT Reagent Kit (Takara, Dalian, China), and miR-1299 was reversed transcribed into cDNA using TaqMan microRNA Reverse Transcription Kit (Invitrogen ...
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bioRxiv - Genomics 2020Quote: First strand cDNA synthesis was performed on ~1μg RNA using the SMARTer PCR cDNA Synthesis Kit (Clontech, UK) followed by PCR amplification with PrimeSTAR GXL DNA Polymerase (Clontech ...
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bioRxiv - Plant Biology 2021Quote: ... Extracted RNA was reverse transcribed using a PrimeScript RT-PCR kit with DNase I (Perfect Real Time) (Takara) in accordance with the accompanying protocol ...
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bioRxiv - Immunology 2021Quote: ... Full-length cDNA was generated using the Clontech SMARTer v4 kit (Takara Bio USA, Inc., Mountain View, CA) prior to library generation with the Nextera XT DNA Library Prep kit (Illumina ...
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bioRxiv - Immunology 2020Quote: ... and purification of cDNA was performed using either the SMART-Seq v4 Ultra Low Input RNA Kit (Takara Bio USA ...
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bioRxiv - Immunology 2020Quote: cDNA from sorted cells was generated using the SMART-Seq v4 Ultra low input RNA kit (Clontech # 634890) according to manufacturer’s instructions ...