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Citations for Takara Bio :
2601 - 2650 of 4900 citations for mu p75 SAP Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2019Quote: ... Reverse-transcription and cDNA amplification were performed with SMARTer® PCR cDNA Synthesis Kit (Clontech Laboratories, Inc.) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: ... cDNA synthesis and amplification was performed using the SMARTer Ultra Low RNA Kit for Illumina sequencing (Clontech) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2019Quote: ... Sequencing libraries were generated using SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian (Takara, 634413). Compared to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2019Quote: ... Y2H assays were performed as recommended in the BD Matchmaker Library Construction and Screening kit (Clontech, USA). The complete coding sequence of PRH1 was fused to the GAL4 DNA binding domain (BD ...
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bioRxiv - Molecular Biology 2019Quote: ... qRT-PCR reactions were performed using a SYBR® Premix Ex Taq™ II Kit (TaKaRa, China) and a CFX96 real-time PCR detection system (BIO-RAD ...
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bioRxiv - Genetics 2019Quote: ... and specific bands were cleaned using NucleoSpin gel clean-up kit (Takara Bio, Mountain View, CA, U.S.A.) for subsequent Sanger sequencing at UMGC ...
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bioRxiv - Cell Biology 2019Quote: Virus titer was determined by quantitative Polymerase Chain Reaction (qPCR) using Adeno-X qPCR Titration Kit (Clontech) on an Applied Biosystems 7900HT.
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bioRxiv - Cell Biology 2019Quote: ... Extracted total RNA were quantified and reverse transcribed into cDNA using the PrimeScript RT Reagent Kit (TaKaRa). All qPCR reactions were performed as 10 μl reactions using TB Green™ Premix Ex Taq™ II (TaKaRa) ...
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bioRxiv - Pathology 2020Quote: ... RBD-scFv was purified from the supernatant using Capturem™ His-Tagged Purification Miniprep Kit (Takara Bio). One prep of 800 μL supernatant through one column of the kit yielded 102 μg/mL of RBD-scFv measured by NanoDrop™ 2000/2000c Spectrophotometers (ThermoFisher) ...
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bioRxiv - Physiology 2019Quote: cDNA from purified RNA were generated using SMART-Seq v4 Ultra Low Input RNA Kit (Takara Clontech). Libraries were made using the Nexterra XT DNA Library Preparation Kit ...
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bioRxiv - Physiology 2019Quote: cDNA from purified RNA were generated using SMART-Seq v4 Ultra Low Input RNA Kit (Takara Clontech). Libraries were made using the Nexterra XT DNA Library Preparation Kit ...
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bioRxiv - Cell Biology 2020Quote: ... This step was performed using commercially available In-Fusion HD Cloning Kit (Clontech, Mountain View, CA, USA). The produced plasmid expresses PRC1 tagged with tgRFPt and SspB at the C-terminus ...
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bioRxiv - Evolutionary Biology 2019Quote: ... qRT-PCR assays were carried out using the SYBR PrimeScript™ RT reagent Kit (TaKaRa, Kusatsu, Japan) following manufacturer‟s instructions and ABI 7500 Software v2.0.6 (Applied Biosystems ...
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bioRxiv - Cell Biology 2019Quote: ... Total 5.0 μg mRNA was used to synthesis cDNA using PrimScript 1st strand cDNA synthesis kit (Takara), and reverse transcription was performed at 45°C for 60 min after 95°C for 5 min ...
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bioRxiv - Plant Biology 2020Quote: ... cDNA was synthesized from 500 ng total RNA using PrimeScript RT reagent Kit (Takara Bio., Shiga, JAPAN). The absence of genomic DNA contamination was confirmed by PCR using a control without reverse transcriptase ...
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bioRxiv - Developmental Biology 2020Quote: Complementary DNAs were prepared by SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Takara 634888) using 150~800pg of total RNA ...
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bioRxiv - Bioengineering 2020Quote: ... Plasmid assembling steps were performed with the In-Fusion HD Cloning Kit (Clontech, Mountain View, CA, USA). The gene cassettes used for the cell-surface expression of hemicellulases were previously optimized [43 ...
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bioRxiv - Neuroscience 2019Quote: ... Lysing of cells and particle purification were carried out using AAVpro® Purification Kit (All Serotypes, TAKARA) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2019Quote: ... The amplified fragments were ligated using an In-Fusion HD cloning kit (Clontech, Mountain View, CA, USA). pcDNA3.1(-)P-gp(MM ...
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bioRxiv - Immunology 2020Quote: ... RNA-Seq libraries were generated using the SMART-Seq v4 Ultra Low Input RNA Kit (Clontech Laboratories) as per the manufacturer’s recommendations ...
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bioRxiv - Genetics 2020Quote: ... according to the manufacturer’s instructions and cDNAs were prepared using PrimeScriptTM RT Master Mix kit (Takara, RR036A). Analysis of mRNA expression was performed with SYBR Green Master Mix (Life Technologies ...
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bioRxiv - Molecular Biology 2019Quote: ... Quantitative PCR was performed using a One Step TB Green PrimeScript RT–PCR Kit II (Takara, #RR086A) on a LightCycler 2.0 instrument (Roche) ...
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bioRxiv - Genomics 2019Quote: ... The extracted RNA was converted to cDNA using SMART-seq v4 Ultra Low input RNA kit (Takara). Then ...
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bioRxiv - Physiology 2021Quote: 200 ng of mRNA were reverse-transcribed to cDNA using the PrimeScriptTM RT reagent kit (RR037A; Takara). Fluorescence-based quantitative real-time PCR (qRT-PCR ...
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bioRxiv - Genetics 2020Quote: ... Purified RNAs were used to construct the library using SMARTer smRNA-Seq Kit for Illumina (Takara, 635029) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... RNA-Seq libraries were generated using the SMART-Seq v4 Ultra Low Input RNA Kit (Clontech Laboratories) according to the manufacturer’s recommendations ...
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Transcriptome Analysis of xa5-mediated Resistance to Bacterial Leaf Streak in Rice (Oryza sativa L.)bioRxiv - Plant Biology 2019Quote: ... First-strand cDNA synthesis was performed using PrimeScript™ RT reagent kit with gDNA eraser (Takara, Japan) following the manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2021Quote: ... Total RNA was extracted from the powder using the NucleoSpin RNA Plant Kit (Takara; http://www.takara-bio.co.jp/), according to the manufacturer’s protocol.
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bioRxiv - Microbiology 2020Quote: ... first-strand cDNA was synthesized by using a PrimeScript RT reagent kit (Perfect Real Time) (TaKaRa Bio) with random hexamer primers and extracted RNA ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... SARS-COV-2 virus detection was performed using the One Step PrimeScript RT-PCR kit (TaKaRa, Japan) on the LightCycler 480 Real-Time PCR system (Roche ...
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bioRxiv - Evolutionary Biology 2020Quote: ... the promoter was inserted into pRS41H-lacZ plasmid using an In-Fusion kit (Takara Bio USA Inc.). The pRS41H-lacZ plasmid was constructed by inserting the lacZ fragment amplified from the pSH18-34 plasmid into the pRS41H plasmid (Estojak et al ...
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bioRxiv - Neuroscience 2020Quote: RNA-seq libraries were prepared using SMARTer Stranded Total RNA-Seq Kit v2 – Pico Input Mammalian (Takara Bio USA ...
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bioRxiv - Developmental Biology 2020Quote: ... The two DNA fragments were then ligated into one plasmid with the InFusion HD cloning kit (Takara).
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bioRxiv - Microbiology 2021Quote: ... total cellular RNA was isolated using the NucleoSpin RNA extraction kit (Macherey-Nagel/Takara, San Jose, California). cDNA was generated from bulk RNA with the high-capacity cDNA reverse transcription kit (Applied Biosystems ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Poly-A selection was conducted on the total RNA using the PrepX PolyA mRNA Isolation Kit (Takara). The mRNA was assessed using the RNA Pico kit (Agilent ...
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bioRxiv - Developmental Biology 2020Quote: ... Both chicken DLX1 and DLX1 Q50E proteins were purified using Capturem His-Tagged Purification Maxiprep Kit (Clontech), and verified by Western blot with primary antibodies against 6x His tag (Invitrogen ...
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bioRxiv - Genomics 2021Quote: ... RNAs were reverse transcribed by using the PrimeScript RT reagent Kit with gDNA Eraser (TAKARA, Kyoto, Japan). Quantification of candidate genes was conducted in triplicate by using Bio-rad iQ SYBR Green Supermix (Bio-rad ...
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Maternal transmission of a paramutant phenotype requires intact DNMT2 functions in the male germlinebioRxiv - Genetics 2020Quote: ... The single cell cDNA libraries were made using SMART-Seq v4 Ultra Low Input RNA Kit (Takara). The cDNAs were fragmentized and Illumina Sequencing adaptors were added using Nextera DNA Library Preparation Kit ...
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bioRxiv - Developmental Biology 2021Quote: ... AAVs particles were then purify from AAV-producing cells using AAVpro Purification Kit Maxi (Takara, Cat. #6666).
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bioRxiv - Genetics 2021Quote: ... First strand cDNA was synthesized using PrimeScript™ RT reagent kit with gDNA Eraser (Takara, Dalian, China). After cDNA synthesis ...
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bioRxiv - Immunology 2021Quote: ... The baculovirus master and working stock titers were determined using rapid titer kit (Clontech, Mountain View, CA). Recombinant baculovirus stocks were prepared by infecting Sf9 cells with a multiplicity of infection (MOI ...
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bioRxiv - Cell Biology 2020Quote: ... Strand specific RNA-seq libraries were then constructed using the SMARTer Stranded RNA-Seq Kit (Clontech # 634839), according to the manufacturer’s protocol ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and was reverse-transcribed into cDNA using a high-capacity cDNA reverse transcription kit (TAKARA, Tokyo, Japan) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... and MSN4 were measured by RT-PCR using One Step SYBR PrimeScript RT–PCR Kit II (TaKaRa). Reactions were run on Mx3005P qPCR System (Agilent Technologies ...
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bioRxiv - Evolutionary Biology 2021Quote: ... we ligated an RNA oligo of the SMART RACE cDNA Amplification Kit (Takara Bio Inc., Shiga, Japan) at 3ʹ-end of the total RNA during reverse transcription ...
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bioRxiv - Neuroscience 2020Quote: ... RNA was extracted and cDNA was synthesized by PCR (PrimeScript RT reagent kit with gDNA Eraser, TaKaRa). SaCas9 was amplified by PCR with Q5 High-Fidelity DNA Polymerase (New England Biolabs ...
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bioRxiv - Developmental Biology 2020Quote: ... Complementary DNAs were prepared by SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Clontech 634888). Post-amplification quality control was performed by Agilent TapeStation DNA High Sensitivity D1000 Screen Tape system ...
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bioRxiv - Cancer Biology 2021Quote: ... Synthesis of Complementary DNA was performed by PrimeScript™ RT reagent kit (Takara Bio Inc., Shiga, Japan), and flowingly used as template for Real-time PCR reaction which was conducted by IQ5 (Biorad ...
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bioRxiv - Developmental Biology 2021Quote: ... RNA were extracted from each well and converted into cDNA using SAMRT-Seq Single cell kit (Takara). DNA library was made and sequenced at Single Cell Genomic Core at the Baylor College of Medicine ...
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bioRxiv - Immunology 2021Quote: ... ds-cDNA and was prepared using the SMARTer Ultra Low RNA kit for Illumina Sequencing (Takara-Clontech) per manufacturer’s protocol ...