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Citations for Takara Bio :
2501 - 2550 of 5037 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: ... and the cDNA was synthesized using the PrimeScript™ RT Reagent Kit with gDNA Eraser (Takara) as recommended by the manufacturer with an oligo dT used instead of random primers ...
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bioRxiv - Developmental Biology 2022Quote: ... cDNA synthesis was performed with SMART- Seq v4 Ultra Low Input RNA Kit (Clontech cat. 634888) according to the manufacturer’s instruction ...
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bioRxiv - Cell Biology 2022Quote: ... qPCR was performed using the SYBR Premix Ex Taq kit (TaKaRa Bio USA, San Jose, CA) and the CFX Connect real-time PCR machine (Bio-Rad ...
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bioRxiv - Genetics 2022Quote: ... and then reverse transcribed RNA to get cDNA using PrimeScript RT reagent kit (Takara, Osaka, Japan). Supplementary Table 1 lists the primers used to construct minigene vectors and to detect alternative splice sites.
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bioRxiv - Cancer Biology 2022Quote: ... cDNA was generated from 1ng of input RNA using the SMART-Seq HT Kit (Takara 634455) at half reaction volume followed by Nextera XT (Illumina FC-131-1024 ...
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bioRxiv - Plant Biology 2022Quote: ... at the SalI and EcoRV restriction sites using an In-Fusion HD Cloning Kit (Takara Bio), which we transferred into the destination vector pGWB50157 using Gateway LR Clonase II Enzyme mix (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2022Quote: ... Total RNA (2 μg) was used for reverse transcription with the PrimeScriptTM RT reagent Kit (TaKaRa). Quantitative expression assays were performed by using the 2x M5 HiPer SYBR Premix EsTag kit (Mei5bio ...
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bioRxiv - Biochemistry 2022Quote: ALP activities were measured using a Great EscAPe™ SEAP kit (Takara Bio Inc., Shiga, Japan) in lysates of PIGT+ and PIGT− cells ...
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bioRxiv - Genomics 2020Quote: ... Libraries were constructed using the Smart cDNA Library Construction kit from Clontech (Palo Alto, CA, USA) and normalized using the Trimmer cDNA Normalization kit from Evrogen (Moscow ...
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bioRxiv - Immunology 2020Quote: ... cDNA was prepared using SMART-Seq v4 Ultra Low Input RNA Kit (Takara Bio USA, Inc). RNA sequencing libraries were prepared using the Illumina TruSeq™ RNA Sample Preparation Kit (Illumina ...
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bioRxiv - Microbiology 2020Quote: ... The qPCR was performed with the MIR-X miRNA qRT-PCR TB Green Kit from TAKARA. To calculate the relative fold of expression of mir-243-3p between generations and genotypes we used delta-delta Ct calculations according to the Reference Unit Mass method (65) ...
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bioRxiv - Molecular Biology 2021Quote: ... cDNA was prepared with 1 µg RNA and synthesized using the Primescript RT kit (Takara Inc.). qPCR assays were performed on the StepOnePlusTM Real-time PCR System (Applied Biosystems ...
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bioRxiv - Plant Biology 2020Quote: ... The extracted RNA was purified using the PrimeScript First Strand cDNA Synthesis Kit (TaKaRa, Dalian, China), and the oligo DT Kit was used to generate cDNA.
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bioRxiv - Neuroscience 2020Quote: ... The titer of lentivirus was approximated by a lentiviral titration kit (Lenti-X GoStix Plus; ClonTech) with serial dilutions ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... total RNA was converted into first strand complementary DNA (cDNA) using a Reverse Transcription Kit (Takara) following the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... m6A and input RNA libraries were prepared using the SMARTer stranded total RNA-seq kit (Clontech) and sequenced on the Illumina NextSeq500 platform (75bp ...
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bioRxiv - Molecular Biology 2021Quote: ... Sequencing reads were trimmed for adapter (AAAAAAAAAA) as recommend by SMARTer smRNA-seq kit (Takara, #635030) protocol using Cutadapt38 (v1.18 ...
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bioRxiv - Microbiology 2021Quote: ... two to four overlapping DNA fragments were PCR amplified (Advantage HF 2 PCR Kit from Clontech) using primers described Table S4 ...
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bioRxiv - Biochemistry 2020Quote: Full-length E protein was synthesized by Invitrogen (GeneArt gene synthesis) and subcloned into KpnI linearized pCAGGS using In-Fusion HD cloning Kit (Takara) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Isolated RNA was used as input for the SMART-seq v4 cDNA synthesis kit (Takara; 634894), and 400 pg of cDNA was used as input for the NexteraXT kit (Illumina) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... PCR products were purified using a NucleoSpin Gel and PCR Clean Up Kit (Takara Bio Inc.). Cycle sequencing reactions were performed directly using the two PCR primers using the BigDye Terminator version 3.1 Cycle Sequencing Kit (Applied Biosystems ...
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bioRxiv - Neuroscience 2020Quote: ... We used the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Takara Cat# 634894) to reverse transcribe poly(A ...
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bioRxiv - Cell Biology 2020Quote: ... and reverse transcription of mRNA was conducted using the PrimeScript™ RT reagent Kit (Takara, RR047Q) with oligo(dT ...
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bioRxiv - Genomics 2021Quote: ... Total RNA (150 ug) was treated using the OligotexTM -dT30
mRNA Purification Kit (Takara Bio). The purified poly(A)+ RNA was reverse-transcribed with oligo(dT ... -
bioRxiv - Pharmacology and Toxicology 2020Quote: ... The mRNA was reverse transcribed to cDNA using a PrimeScript RT reagent kit (Takara; Dalian, China). Real-time reverse transcription quantitative PCR (qPCR ...
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bioRxiv - Neuroscience 2021Quote: ... ds-cDNA was prepared using the SMARTer Ultra Low RNA kit for Illumina Sequencing (Takara-Clontech) per manufacturer’s protocol using the lysis buffer as substrate for the reaction ...
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bioRxiv - Immunology 2020Quote: ... The target band was excised and purified with a DNA gel recovery kit (TaKaRa, Dalian, China), according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... qRT-PCR analysis was performed using cDNA synthesized by the Prime Script RT Reagent Kit (TaKaRa). Each qRT-PCR was conducted in a 20-μL reaction mixture containing 2 μL of diluted template cDNA ...
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bioRxiv - Biochemistry 2021Quote: ... The PCR product was purified using a NucleoSpin Gel and PCR Clean-up kit (Takara Bio), and Sanger sequencing was performed using primers 134 ...
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bioRxiv - Microbiology 2022Quote: ... The titres of the generated baculovirus were determined using BacPAK Baculovirus rapid titre kit (Clontech, USA).
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bioRxiv - Evolutionary Biology 2021Quote: ... and used as a template in reverse transcription with the SMART cDNA Library Construction Kit (Clontech). cDNA fragments amplified using the degenerate primers designed based on the ortholog sequences of close relatives were sequenced with a 3730xl DNA Analyzer (Thermo Fisher Scientific) ...
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bioRxiv - Developmental Biology 2020Quote: ... RNA-seq libraries were generated using the SMARTer Stranded Total RNA Sample Prep Kit (Takara Bio) and sequenced using NextSeq500/550 High Output v2.5 kit to a minimum depth of 25 million reads per sample ...
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bioRxiv - Biophysics 2021Quote: ... The 7-kb band was excised and recycled using Agarose Gel DNA Extraction Kit (TaKaRa Bio). Concurrently ...
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bioRxiv - Cell Biology 2020Quote: ... was performed using the In-Fusion Cloning Kit and online tools (BD Clontech, Takara Bio, USA). All plasmids were verified by sequencing (Genewiz ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... and cDNA was synthesized using a PrimeScript RT reagent kit with gDNA Eraser (Takara Bio Inc.). Gene-specific primers for the complete ORF of IDH were designed based on partial sequences of Unigene 32825 obtained from the transcriptome data of the heads of O ...
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bioRxiv - Cell Biology 2021Quote: ... Heisenberg lab) was inserted downstream of the srpHemo promoter fragment using the infusion kit from Clontech with the following primer pair ...
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bioRxiv - Biochemistry 2020Quote: ... Genomic DNA was extracted from the buffy coat using the NucleoSpin Tissue kit (Takara, Shiga, Japan). Bisulfite conversion was performed using the Epitect Bisulfite Kit (Qiagen ...
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bioRxiv - Neuroscience 2021Quote: ... RNA was transcribed to cDNA with the PrimeScript™ RT reagent Kit (Perfect Real Time, Takara) and reactions were performed with SYBR® Premix Ex Taq™ (Takara ...
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bioRxiv - Microbiology 2020Quote: ... Libraries were prepared with the SMARTer PrepX DNA Library Kit (Takara Bio, Mountain View, CA, USA), using the Apollo NGS library prep system (Takara Bio ...
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bioRxiv - Microbiology 2020Quote: ... and then cDNAs were produced using the PrimeScript RT reagent kit with gDNA Eraser (TaKaRa, RR047A). For viral DNA load quantification in whitefly whole bodies ...
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bioRxiv - Microbiology 2020Quote: The cDNA was purified using a NucleoSpin Gel and PCR Clean-up kit (Takara Bio, Inc.). PCR was performed with the cDNA ...
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bioRxiv - Immunology 2020Quote: ... PCR samples were indexed with Nextera Illumina Indices reads using the Advantage 2 PCR kit (Clontech) in a 8 PCR cycle reaction and purified with Agencourt AMPpure XP beads (Beckman Coulter ...
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bioRxiv - Immunology 2020Quote: ... RNA-seq libraries were generated using the SMART-Seq-v4-Ultra Low Input RNA Kit (Clontech) combined to the Nextera library preparation kit (Illumina ...
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bioRxiv - Immunology 2020Quote: TCR repertoire profiling was performed using the SMARTer TCR α/β Profiling Kit (Takara Bio, USA) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... cDNA was synthesized using the SMART-Seq v4 Ultra Low Input RNA Kit (Takara Bio USA), and barcoded libraries were prepared using the Nextera XT DNA Library Preparation Kit (Illumina) ...
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bioRxiv - Immunology 2021Quote: ... AAV6 vector genomes were titrated by ITR-specific quantitative PCR (Takara AAVpro Titration Kit Ver.2) per the manufacturer’s protocol.
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bioRxiv - Immunology 2021Quote: ... RNA was converted to cDNA with the SMART-Seq v4 Ultra Low Input RNA kit (Takara) and converted into sequencing libraries using the DNA Nano Kit (Illumina) ...
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bioRxiv - Immunology 2020Quote: ... The cDNA was generated by employing ingredients from the TAKARA cDNA Synthesis Kit (TAKARA Bio Europe), based on the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... The PCR was performed with the One Step TB Green PrimeScript RT-PCR Kit II (TaKaRa), and SYBR Green was used as a fluorescent dye for detection ...
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bioRxiv - Immunology 2021Quote: ... and complementary DNA was synthesized with a PrimeScript™ RT reagent Kit with gDNA Eraser (Takara). The target genes were normalized to the housekeeping gene (Gapdh ...