Labshake search
Citations for Takara Bio :
2501 - 2550 of 5125 citations for Medium Kit without Serum and with CultureBoost since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2021Quote: ... DNA was prepared using the endotoxin-free NucleoBond Xtra Maxi EF kit (Clontech, Takara Bio, USA).
-
bioRxiv - Microbiology 2021Quote: ... cDNA was synthesized from total RNA using a PrimeScript Perfect real-time reagent kit (Takara Bio) according to the instructions provided by the manufacturer ...
-
bioRxiv - Developmental Biology 2022Quote: ... and the cDNA was synthesized using the PrimeScript™ RT Reagent Kit with gDNA Eraser (Takara) as recommended by the manufacturer with an oligo dT used instead of random primers ...
-
bioRxiv - Developmental Biology 2022Quote: ... cDNA synthesis was performed with SMART- Seq v4 Ultra Low Input RNA Kit (Clontech cat. 634888) according to the manufacturer’s instruction ...
-
bioRxiv - Cell Biology 2022Quote: ... qPCR was performed using the SYBR Premix Ex Taq kit (TaKaRa Bio USA, San Jose, CA) and the CFX Connect real-time PCR machine (Bio-Rad ...
-
bioRxiv - Genetics 2022Quote: ... and then reverse transcribed RNA to get cDNA using PrimeScript RT reagent kit (Takara, Osaka, Japan). Supplementary Table 1 lists the primers used to construct minigene vectors and to detect alternative splice sites.
-
bioRxiv - Cancer Biology 2022Quote: ... cDNA was generated from 1ng of input RNA using the SMART-Seq HT Kit (Takara 634455) at half reaction volume followed by Nextera XT (Illumina FC-131-1024 ...
-
bioRxiv - Plant Biology 2022Quote: ... at the SalI and EcoRV restriction sites using an In-Fusion HD Cloning Kit (Takara Bio), which we transferred into the destination vector pGWB50157 using Gateway LR Clonase II Enzyme mix (Thermo Fisher Scientific) ...
-
bioRxiv - Microbiology 2022Quote: ... Total RNA (2 μg) was used for reverse transcription with the PrimeScriptTM RT reagent Kit (TaKaRa). Quantitative expression assays were performed by using the 2x M5 HiPer SYBR Premix EsTag kit (Mei5bio ...
-
bioRxiv - Biochemistry 2022Quote: ALP activities were measured using a Great EscAPe™ SEAP kit (Takara Bio Inc., Shiga, Japan) in lysates of PIGT+ and PIGT− cells ...
-
bioRxiv - Molecular Biology 2022Quote: ... The protein concentration was found to be 0.9 µg/µl using BCA Protein Assay Kit (TaKaRa).
-
bioRxiv - Microbiology 2019Quote: ... and reverse transcription-PCR (RT-PCR) was carried out with a PrimeScript RT reagent kit (TaKaRa). The primers used for RT-PCR are listed in Table 3 ...
-
bioRxiv - Genomics 2020Quote: ... Libraries were constructed using the Smart cDNA Library Construction kit from Clontech (Palo Alto, CA, USA) and normalized using the Trimmer cDNA Normalization kit from Evrogen (Moscow ...
-
bioRxiv - Neuroscience 2019Quote: ... AAVs were collected from HEK293T cells and purified using AAVpro Purification Kit (Takara Bio, Shiga, Japan) according to the manufacturer’s protocol ...
-
bioRxiv - Immunology 2019Quote: ... Reverse transcription was performed to generate cDNA by using PrimeScriptTM RT reagent Kit (Takara, cat. # RR047A). Quantitative PCR was performed on LC480 (Roche ...
-
bioRxiv - Evolutionary Biology 2019Quote: ... and used to make transcriptome libraries using the PrepX RNA-Seq for Illumina Library Kit (Takara). The HS DNA Kit (Agilent ...
-
bioRxiv - Plant Biology 2019Quote: ... cDNA was synthesized from 500 ng of DNA-free RNA using PrimeScript RT reagent Kit (Takara), supplemented with RNase out (Life Technologies).
-
bioRxiv - Immunology 2019Quote: ... and cDNA was generated using SMART Seq® v4 Ultra™ Low Input RNA Kit (Clontech), both according to manufacturer’s instructions ...
-
bioRxiv - Microbiology 2019Quote: ... cDNA was synthesized with 100 ng RNA using Advantage RT-PCR Kit (Clontech, Mountain View, USA). Reaction condition for real time PCR was 95°C for 3min (1 cycle) ...
-
bioRxiv - Immunology 2019Quote: ... RACE-ready cDNA synthesis was performed using the SMARTer RACE 5’/3’ Kit (Takara Bio USA) using primers with specificity to IgM ...
-
Cholesterol deprivation induces TGFβ signaling to promote basal differentiation in pancreatic cancerbioRxiv - Cancer Biology 2019Quote: ... Total RNA libraries were prepared by using Pico Input SMARTer Stranded Total RNA-Seq Kit (Takara). In short ...
-
bioRxiv - Immunology 2020Quote: ... cDNA was prepared using SMART-Seq v4 Ultra Low Input RNA Kit (Takara Bio USA, Inc). RNA sequencing libraries were prepared using the Illumina TruSeq™ RNA Sample Preparation Kit (Illumina ...
-
bioRxiv - Microbiology 2020Quote: ... The qPCR was performed with the MIR-X miRNA qRT-PCR TB Green Kit from TAKARA. To calculate the relative fold of expression of mir-243-3p between generations and genotypes we used delta-delta Ct calculations according to the Reference Unit Mass method (65) ...
-
bioRxiv - Molecular Biology 2021Quote: ... cDNA was prepared with 1 µg RNA and synthesized using the Primescript RT kit (Takara Inc.). qPCR assays were performed on the StepOnePlusTM Real-time PCR System (Applied Biosystems ...
-
bioRxiv - Plant Biology 2020Quote: ... The extracted RNA was purified using the PrimeScript First Strand cDNA Synthesis Kit (TaKaRa, Dalian, China), and the oligo DT Kit was used to generate cDNA.
-
bioRxiv - Neuroscience 2020Quote: ... The titer of lentivirus was approximated by a lentiviral titration kit (Lenti-X GoStix Plus; ClonTech) with serial dilutions ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... total RNA was converted into first strand complementary DNA (cDNA) using a Reverse Transcription Kit (Takara) following the manufacturer’s instructions ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... m6A and input RNA libraries were prepared using the SMARTer stranded total RNA-seq kit (Clontech) and sequenced on the Illumina NextSeq500 platform (75bp ...
-
bioRxiv - Molecular Biology 2021Quote: ... Sequencing reads were trimmed for adapter (AAAAAAAAAA) as recommend by SMARTer smRNA-seq kit (Takara, #635030) protocol using Cutadapt38 (v1.18 ...
-
bioRxiv - Molecular Biology 2019Quote: ... Library preparation was done with the ThruPLEX DNA-Seq kit (Takara Bio, Mountain View, CA, USA) according to the recommended protocol and 18 amplification cycles ...
-
bioRxiv - Cell Biology 2019Quote: Total RNA was extracted from cultured cells using Takara MiniBest Universal RNA Extraction Kit (Takara, 9767). cDNA was synthesized through RevertAid First Strand cDNA Synthesis Kit (Thermo Scientific ...
-
bioRxiv - Plant Biology 2019Quote: ... The cDNA was synthesized using the PrimeScript RT reagent Kit with gDNA Eraser (Takara, Dalian, China). Quantitative real time polymerase chain reaction (qRT-PCR ...
-
bioRxiv - Microbiology 2021Quote: ... two to four overlapping DNA fragments were PCR amplified (Advantage HF 2 PCR Kit from Clontech) using primers described Table S4 ...
-
bioRxiv - Genomics 2019Quote: ... Isolation of total RNA from plant materials was performed using an RNA extraction kit (Takara, Japan) according to the manufacturer’s recommendations ...
-
bioRxiv - Synthetic Biology 2019Quote: ... Linear templates were cloned into the pY71 vector using the In-fusion seamless cloning kit (Clontech). Circular templates were amplified in E ...
-
bioRxiv - Plant Biology 2019Quote: ... fragments were amplified by PCR and ligated using the In-Fusion HD Cloning Kit (Takara Bio). Using RTS 100 Wheat Germ CECF Kit (Roche) ...
-
bioRxiv - Physiology 2019Quote: Total RNA extraction was performed using NucleoSpin RNA XS kit (Clontech Laboratories Inc. Cat. No. 740902). Briefly ...
-
bioRxiv - Pharmacology and Toxicology 2019Quote: ... were used for each reaction and amplified by KOD Xtreme Hot Start DNA polymerase kit (Takara). The PCR cycling conditions used are ...
-
bioRxiv - Biochemistry 2020Quote: Full-length E protein was synthesized by Invitrogen (GeneArt gene synthesis) and subcloned into KpnI linearized pCAGGS using In-Fusion HD cloning Kit (Takara) according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2021Quote: ... Isolated RNA was used as input for the SMART-seq v4 cDNA synthesis kit (Takara; 634894), and 400 pg of cDNA was used as input for the NexteraXT kit (Illumina) ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... PCR products were purified using a NucleoSpin Gel and PCR Clean Up Kit (Takara Bio Inc.). Cycle sequencing reactions were performed directly using the two PCR primers using the BigDye Terminator version 3.1 Cycle Sequencing Kit (Applied Biosystems ...
-
bioRxiv - Neuroscience 2020Quote: ... We used the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Takara Cat# 634894) to reverse transcribe poly(A ...
-
bioRxiv - Cell Biology 2020Quote: ... and reverse transcription of mRNA was conducted using the PrimeScript™ RT reagent Kit (Takara, RR047Q) with oligo(dT ...
-
bioRxiv - Genomics 2021Quote: ... Total RNA (150 ug) was treated using the OligotexTM -dT30
mRNA Purification Kit (Takara Bio). The purified poly(A)+ RNA was reverse-transcribed with oligo(dT ... -
bioRxiv - Pharmacology and Toxicology 2020Quote: ... The mRNA was reverse transcribed to cDNA using a PrimeScript RT reagent kit (Takara; Dalian, China). Real-time reverse transcription quantitative PCR (qPCR ...
-
bioRxiv - Neuroscience 2021Quote: ... ds-cDNA was prepared using the SMARTer Ultra Low RNA kit for Illumina Sequencing (Takara-Clontech) per manufacturer’s protocol using the lysis buffer as substrate for the reaction ...
-
bioRxiv - Immunology 2020Quote: ... The target band was excised and purified with a DNA gel recovery kit (TaKaRa, Dalian, China), according to the manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2021Quote: ... qRT-PCR analysis was performed using cDNA synthesized by the Prime Script RT Reagent Kit (TaKaRa). Each qRT-PCR was conducted in a 20-μL reaction mixture containing 2 μL of diluted template cDNA ...
-
bioRxiv - Biochemistry 2021Quote: ... The PCR product was purified using a NucleoSpin Gel and PCR Clean-up kit (Takara Bio), and Sanger sequencing was performed using primers 134 ...
-
bioRxiv - Microbiology 2022Quote: ... The titres of the generated baculovirus were determined using BacPAK Baculovirus rapid titre kit (Clontech, USA).