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Citations for Takara Bio :
2301 - 2350 of 5125 citations for Medium Kit without Serum and with CultureBoost since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: Rapid amplification of cDNA ends was performed using the SMARTerR RACE 5’/3’kit (Takara), us 1 μg of DNA-free RNA from zeocin-treated samples as template for first-strand cDNA synthesis according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Extracted and purified RNA was then converted to cDNA using PrimeScript cDNA synthesis kit (TaKaRa) according to the manufacture’s instruction ...
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bioRxiv - Neuroscience 2023Quote: ... which was inserted into pAcGFP-His- MAP2C 11-308 by In-Fusion cloning kit (Takara). pAcGFP-His-MAP2C-Tau was chimera of MAP2C_M1-L311 and Tau0N4R_P193-L383 in the numbering Tau0N4R ...
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bioRxiv - Genetics 2023Quote: ... and libraries were prepared using the SMART-Seq v4 Ultra Low Input RNA kit (TaKaRa). Sequencing was performed on an Illumina NextSeq at the Harvard Bauer core facility ...
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bioRxiv - Neuroscience 2022Quote: The cDNA samples were prepared using a Prime-Script RT reagent Kit (TaKaRa, Dalian, China) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... followed by the preparation of cDNA using Prime Script™ RT Reagent kit (Takara Bio) as per the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... genomic phage DNA was extracted with MiniBEST Viral RNA/DNA Extraction Kit (Takara, Beijing, China) following manufacturer’s protocol ...
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bioRxiv - Bioengineering 2023Quote: ... The RNA samples were reverse transcribed using a PrimeScript RT Reagent kit (Takara Bio Inc.). Quantitative real-time PCR was performed using THUNDERBIRD™ Probe qPCR Mix or THUNDERBIRD™ SYBR qPCR Mix (TOYOBO ...
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bioRxiv - Plant Biology 2023Quote: ... and cDNA was synthesized from 500 ng of RNA using Primescript RT Reagent Kit (Takara) with random hexamer primers ...
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bioRxiv - Microbiology 2023Quote: ... First-strand cDNA was synthesized with the FastQuant RT Kit (Takara biomedical technology, Beijing, China) following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... First-strand cDNA synthesis was performed using the Super Smart cDNA Synthesis kit (Takara, Japan). Gene-specific primers (Table S1 ...
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bioRxiv - Immunology 2023Quote: ... total RNA underwent amplification using the SMART-Seq v4 Ultra Low Input RNA Kit (Clontech), and amplified cDNA was used to prepare libraries with the KAPA Hyper Prep Kit (Kapa Biosystems) ...
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bioRxiv - Genomics 2023Quote: ... purified RNA were constructed with the SMARTer Stranded RNA-seq Kit (Takara, cat. no. #634839) according to the manufacturer’s instructions for first- strand cDNA synthesis ...
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bioRxiv - Cell Biology 2023Quote: ... cDNA was obtained by reverse transcription using the PrimeScript RT Reagent Kit (Takara, Kusatsu, Japan). RT-qPCR was performed with the respective human-specific sense and antisense primers and RT-SYBR™ Green PCR Master Mix (Applied Biosystems) ...
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bioRxiv - Cell Biology 2023Quote: ... cDNA was synthesized using SMARTer Ultra Low RNA Kit for Illumina Sequencing (634936, Takara Bio). The DNA libraries were constructed using TruePrep DNA Library Prep Kit V2 for Illumina (TD503-02 ...
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bioRxiv - Developmental Biology 2023Quote: ... Total RNA from morulas was obtained using the NucleoSpin RNA Plus XS kit (Takara, 740990.10). Whereas total RNA from blastocyst was extracted using the Arcturus PicoPure RNA Isolation Kit (Thermo Fisher ...
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bioRxiv - Neuroscience 2023Quote: ... and the resulting titers estimated using the Lenti-X qRT-PCR Titration Kit (Takara Bio), all following manufacturers’ recommendations ...
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bioRxiv - Microbiology 2023Quote: ... coli-Streptococcus shuttle vector pDL278 by using an In-Fusion HD cloning kit (TaKaRa, Japan) as described previously [49] ...
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bioRxiv - Pathology 2023Quote: ... cDNA was prepared from 1µg RNA using PrimeScriptTM RT reagent Kit with gDNA Eraser (TaKaRa). RT- qPCR was performed using 2.5 μL of cDNA ...
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bioRxiv - Cell Biology 2023Quote: ... and were reversed transcribed into first-strand cDNA using the PrimeScript RT Reagent Kit (Takara) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: ... according to the steps in the instruction book of SYBR PrimeScript RT-PCR kit (Takara). We used 2-ΔΔc to indicate the relative gene expression level of each sample.
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bioRxiv - Plant Biology 2023Quote: ... Protein concentration was measured using a BCA assay kit (Takara Bio Inc., Kusatsu, Siga, Japan). Twenty micrograms (µg ...
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bioRxiv - Microbiology 2023Quote: ... 2×SYBR Green PCR Mastermix (RR820A) and reverse transcription kit (RR047A) were purchased from TAKARA company ...
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bioRxiv - Genomics 2023Quote: ... The RT-PCR products were treated with NucleoSpin Gel and PCR Cleanup kit (Takara Bio) and directly analyzed using Sanger sequencing (Eurofins Genomics) ...
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bioRxiv - Neuroscience 2023Quote: ... RNA-seq libraries were constructed using SMARTer Pico-Input stranded RNA-seq kit (Takara, 634836). For input RNA samples ...
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bioRxiv - Molecular Biology 2023Quote: ... The price of SMARTer Stranded Total RNA-Seq Kit v2-Pico Input Mammalian (TaKaRa, 634413) was searched on the official website of TaKaRa for the estimation of SMARTer-seq ...
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bioRxiv - Cancer Biology 2023Quote: ... cDNA was amplified by 15 cycles of PCR using the Advantage 2 PCR Kit (Clontech) with template switching custom oligo (TableS6) ...
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bioRxiv - Bioengineering 2022Quote: ... RNA to cDNA conversion was performed using the SMART cDNA Library Construction Kit by Clontech Laboratories ...
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bioRxiv - Cell Biology 2022Quote: ... by double-insert cloning using an In-Fusion HD Cloning Kit (#Z9648N; TaKaRa, Kusatsu, Japan). The pcDNA5 FRT/TO HA FLAG Hu AASS was then digested with BamHI/ApaI and the DNA fragment was ligated into the pcDNA5 FRT/TO vector using a DNA Ligation Kit (#6023 ...
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bioRxiv - Developmental Biology 2023Quote: ... the RNA was immediately used for library preparation using the SMARTer smRNA-Seq Kit (Takara), with the following modifications ...
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bioRxiv - Developmental Biology 2023Quote: ... Genotyping was done using the Terra™ PCR Direct kit (Takara Bio, Kusatsu, Shiga Japan). Embryonic sex and Lhx2 allele genotyping were performed as mentioned [26] ...
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bioRxiv - Cancer Biology 2022Quote: ... TUNEL assay was carried out using in-situ apoptosis detection kit (Takara Bio Inc, Japan), as per the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: In-fusion cloning was performed using the In-Fusion HD EcoDry Cloning Plus kit (Takara), and standard cut-and-paste cloning using T7 DNA ligase (New England Biolabs) ...
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bioRxiv - Neuroscience 2023Quote: ... cDNA was synthesized using the SMART-Seq v4 Ultra Low input RNA Kit (Takara 634892). Sequencing libraries were made with the NEBNext Ultra II DNA Library Prep kit (New England Biolabs E7645L ...
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bioRxiv - Cancer Biology 2023Quote: ... The virus titer was determined with Lenti-X™ p24 Rapid Titration ELISA Kit (Takara), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... mRNA libraries were prepared using the SMART-Seq v4 Ultra Low Input RNA Kit (Takara) per manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... cDNA was synthesized from RNA using the Prime script 1st-strand cDNA synthesis kit (Takara) as per the manufacturer’s instructions and using a mixture of random hexamer and oligo dT primers ...
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bioRxiv - Cell Biology 2023Quote: ... Each cDNA was synthesized using a PrimeScript RT reagent kit (Takara Bio, Kusatsu-Shiga, Japan). Quantitative PCR reactions were performed using SYBR Premix Ex Taq II with a thermal cycler (TP800 ...
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bioRxiv - Microbiology 2023Quote: ... cDNA was synthetized from total RNA using PrimeScript II 1st Strand cDNA Synthesis Kit (Takara) and amplified using ChamQ Universal SYBR qPCR Master Mix (Vazyme Biotech ...
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bioRxiv - Cell Biology 2023Quote: ... cDNA libraries were generated using the Small RNA-Seq Kit for Illumina (Takara, Shiga, Japan). cDNA fragments are sequenced by the read length using sequence by synthesis method on the Illumina platform ...
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bioRxiv - Cell Biology 2023Quote: ... genomic DNA was extracted and purified using a Nucleospin Blood XL kit (Takara Bio, #740950.10). The guides were amplified and barcoded by PCR using NEB Next Ultra ii Q5 MM (M0544L) ...
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bioRxiv - Microbiology 2023Quote: ... Purified RNA product was immediately used with the One-step PrimeScript RT-PCR Kit (Takara). Primers and probes were obtained from IDT ...
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bioRxiv - Molecular Biology 2023Quote: ... cDNA was prepared from equal amounts of RNA using PrimeScript RT Reagent Kit (Takara, RR037A) following manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... and introduced into PstI site of the pSXY1033 with In-Fusion HD cloning kit (Takara) to generate transformation vectors pSXY1034 to pSXY1039 (Seq ...
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bioRxiv - Neuroscience 2023Quote: ... The virus titer was measured using Lenti-X GoStix Plus assay kit (Takara Bio, 631280).
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bioRxiv - Genomics 2023Quote: ... RNA-seq libraries were prepared using SMARTer Stranded total RNA-seq Kit v3 (Clontech, 634486) per the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... the RNA was processed using the SMARTer Stranded Total RNA-Seq Kit v2 (Takara, #634411). The fragment length of the libraries was verified using the Fragment Analyzer 12 (Agilent) ...
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bioRxiv - Neuroscience 2023Quote: ... mRNA amplification and cDNA preparation were performed using the SMARTer mRNA amplification kit (Takara #634940). Labeled samples were sequenced using an Illumina NextSeq 500 sequencer using 75 base pair single read protocols.
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bioRxiv - Bioengineering 2023Quote: ... GFP BrightComp eBeads™ Compensation Bead Kit (ThermoScientific) and mCherry Flow Cytometer Calibration Beads (Takara). Flow cytometry of stained T lymphocytes was then performed using a BD LSRFortessa Cell Analyzer (BD Biosciences ...
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bioRxiv - Cell Biology 2023Quote: ... 500 ng total RNA was reversed transcribed into cDNA using the cDNA synthesis kit (Takara). The cDNA was diluted for suitable fold and mixed with qPCR SYBR Green Master Mix (Takara ...