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Citations for Takara Bio :
2401 - 2450 of 6736 citations for Onion Yellow Dwarf Virus OYDV PCR Kits since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... 3C protease-cleavage site and a His12-tag at the N-terminus were amplified by PCR using KOD-Plus-Neo polymerase (Toyobo) and Human Universal QUICK-Clone cDNA II (Clontech) as a template cDNA and then cloned into a pET-41 Ek/LIC vector (Novagen) ...
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bioRxiv - Plant Biology 2021Quote: ... captured DNA fragments were PCR-amplified using Takara LA Taq DNA Polymerase over 15 cycles (Clontech/Takara Bio, Otsu, Japan) then purified using AMPure PB Beads (Beckman Coulter ...
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bioRxiv - Neuroscience 2021Quote: ... a DNA fragment of the TkR86C coding region was amplified from cDNA from the Canton-S wild type strain by PCR (PrimeSTAR GXL, TaKaRa Clontech) with primers that had NotI and XbaI sites at 5’ and 3’ ends ...
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bioRxiv - Cell Biology 2021Quote: ... The PCR product and the pMECS phagemid vector were digested sequentially with Pst I and Not I (TaKaRa, Dalian, China) and then ligated by T4 DNA ligase at 16°C overnight ...
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C53 interacting with UFM1-protein ligase 1 regulates microtubule nucleation in response to ER stressbioRxiv - Cell Biology 2020Quote: ... The complete sequence of CDK5RAP3 without the stop codon was excised from pCR-hCDK5RAP3 by NheI/SalI and ligated into pEGFP-N3 (Clontech), resulting in plasmid phCDK5RAP3-EGFP ...
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bioRxiv - Developmental Biology 2022Quote: ... 1µl diluted cDNA was used as the template for each reaction with Emerald Amp GT PCR Master Mix (Takara, RR310). After appropriate amplification cycles ...
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bioRxiv - Molecular Biology 2022Quote: ... gDNA extracted and PCR genotyped with primers (Supplemental table 1) flanking each homologous arms using PrimeStar GXL DNA polymerase (Takara). Correctly targeted clones were further expanded and banked ...
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bioRxiv - Biochemistry 2022Quote: ... and the linearized plasmid gel-extracted and assembled with PCR-amplified codon-optimized mNeongreen or mScarlet using In-fusion cloning (Takara). To generate pPD95.75-itr- 1p-mNG or pPD95.75-itr-1p-mSc plasmids pPD95.75-mNG plasmid was digested with HindIII and PstI ...
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bioRxiv - Microbiology 2022Quote: ... Quantitative real-time RCR (qRT-PCR) was carried-out using SYBR Premix Ex Taq™ (Tli RNaseH Plus) (TaKaRa, Japan) and CFX96TM real-time PCR system (Biorad ...
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bioRxiv - Microbiology 2022Quote: ... covering the full-length SARS-CoV- 2 genome were amplified by PCR using a PrimeSTAR GXL DNA polymerase (TaKaRa Bio), the synthesized cDNA and specific primer sets from CoV-2-G1-Fw to CoV-2-G10-Rv designed previously (21) ...
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bioRxiv - Genetics 2022Quote: ... The protein-coding genomic sequence including 3 kbp promoter sequence was PCR-amplified and cloned into the pGreen0029 binary vector (Hellens et al., 2000) using In-Fusion Cloning system (Takara Bio Europe ...
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bioRxiv - Microbiology 2022Quote: ... 16S rRNA gene sequences were amplified by polymerase chain reaction (PCR) using LA Taq polymerase (TaKaRa-Bio, Inc., Kusatsu, Japan) for Illumina MiSeq paired-end sequencing ...
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bioRxiv - Cancer Biology 2022Quote: ... cDNAs were diluted 1/10 and PCR reactions were conducted using SYBR qPCR Premix Ex Taq II Tli RNase H+ (TAKRR820W, Takara). Primers can be found in Supplementary Table 4 ...
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bioRxiv - Cell Biology 2022Quote: ... The cDNA was amplified via PCR and cloned into the appropriate vector backbones (empty pCS2+ or pCS2+ with C-terminal 3xeGFP) using InFusion Assembly (Takara). The GAP-dead mutant (RGA-3/4R80E ...
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bioRxiv - Cell Biology 2022Quote: pLVX-Puro GFP-TMEM11 was generated by PCR amplifying TMEM11 from human cDNA and cloning into the XhoI/BamHI sites of pAcGFP1-C1 (Takara), followed by subsequent cloning of the GFP-TMEM11 cassette into the Xho/BamHI sites of pLVX-Puro (Takara ...
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bioRxiv - Plant Biology 2021Quote: ... The CDS of the NON-RIPENING gene (NOR, Solyc10g006880) was amplified from the synthesized cDNA by PCR using PrimeSTAR GXL DNA Polymerase (TaKaRa) and the primer set SlNOR-pPLV26-F/R (Supplementary Table S11) ...
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bioRxiv - Molecular Biology 2021Quote: ... Quantitative real-time PCR reactions were performed using 1/60 of the immunoprecipitated DNA and the TB Green Premix Ex Taq II (Takara). Relative quantification using standard curve method was performed for each primer pair and 96-well Optical Reaction plates were read in a StepOnePlus PCR instrument (Applied Biosystem) ...
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A Phytochrome B-PIF4-MYC2 Module Tunes Secondary Cell Wall Thickening in Response to Shaded LightingbioRxiv - Plant Biology 2021Quote: ... N-terminal containing TAD and C-terminal containing bHLH of PIF4 were PCR-amplified and fused with GAL4 activation domain (AD) of the pray vector pGADT7 (Clontech). Bait and pray vectors were co-transformed in to the Y2H Gold yeast strain ...
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bioRxiv - Microbiology 2021Quote: ... Real-time PCR was performed using the THUNDERBIRD SYBR qPCR Mix (Toyobo) and the Thermal Cycler Dice Real Time System II (Takara). The transcript level in each strain was normalized to the internal control (rnpB) ...
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bioRxiv - Microbiology 2020Quote: ... The circularized DNA was subjected to PCR to generate a sequencing library using Illumina index primers and PrimeSTAR Max DNA polymerase (Takara). The PCR product was loaded and electrophoresed by 8% TBE PAGE ...
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bioRxiv - Plant Biology 2021Quote: ... captured DNA fragments were PCR-amplified using Takara LA Taq DNA Polymerase over 15 cycles (Clontech/Takara Bio, Otsu, Japan) then purified using AMPure PB Beads (Beckman Coulter ...
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bioRxiv - Immunology 2020Quote: ... Detection of the number of copies of extracted RNA was performed using the Real-Time One-Step RT-PCR reagent (Takara). The following was the reaction system ...
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bioRxiv - Biochemistry 2020Quote: ... complementary BsmBI sites to those on the OLS fragments of a respective subpool were added by PCR using Primerstar GXL DNA polymerase (Takara) and 100 pg of wildtype channel as template DNA (Supplemental Figure S3A) ...
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bioRxiv - Microbiology 2021Quote: ... P3 and NP from D/660 and D/CN286 were amplified from the respective bidirectional pHW2000 plasmid constructs using CloneAmp Hifi PCR mix (Takara) with the following cycle profile ...
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bioRxiv - Plant Biology 2021Quote: ... and ΔRST (missing the residues 462-589) deletions were introduced by PCR using primers listed in Supplementary table 3 and end-joining using In-Fusion (Clontech).
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bioRxiv - Plant Biology 2021Quote: ... and ΔRST (missing the residues 462-589) deletions were introduced by PCR using primers listed in Supplementary table 3 and end-joining using In-Fusion (Takara).
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bioRxiv - Microbiology 2022Quote: ... A second construct for total gene deletion was generated by cloning the 5’ (843 bp) and 3’ (292 bp) homology regions amplified by PCR using a Taq DNA polymerase with proofreading activity (Takara) and primers P9 + P12 and P13 + P14 into the pL0001 vector (BEI Resources ...
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bioRxiv - Immunology 2022Quote: ... was cloned from pHRSIN (kind gift from Prof. Alain Townsend) into the Addgene 17448 lentivirus vector by InFusion PCR (TaKaRa) and verified by Sanger sequencing ...
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bioRxiv - Microbiology 2022Quote: ... Seven Ns were added into the primers as the barcode for barcoded-subamplicon sequencing during the first round PCR (PrimeSTAR Max DNA Polymerase, Takara Bio ...
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bioRxiv - Microbiology 2022Quote: The following plasmids were generated by subcloning polymerase chain-reaction (PCR) generated amplicons from the indicated oligonucleotides from Table 1 into pEFtak or pLEX vector using In-fusion recombinase (Takara) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... Each PCR product was subcloned into the NheI and XhoI sites of a cytomegalovirus promoter-driven pIRES2-AcGFP1 vector (Clontech Laboratories Inc. ...
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bioRxiv - Genomics 2020Quote: ... and quantitative real time PCR was performed following manufacturer’s instructions given in SYBR1 Premix ExTaq (TliRNAse H Plus) (Clontech,USA). The PCR amplification was carried out in Rotor-Gene-Q Real-Time PCR System (Qiagen ...
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bioRxiv - Synthetic Biology 2020Quote: ... Homology arms were assembled into digested (SacI, XbaI) or PCR-amplified pLO3 backbone via Gibson Assembly (HiFi, NEB or In-fusion, Takara). C ...
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bioRxiv - Cell Biology 2020Quote: ... Mouse E-Syt3 cDNA was amplified by PCR from a cDNA library constructed with poly(A) RNA from 3T3-L1 adipocytes (Mate & Plate TM Library System, Clontech) and cloned into the PT-Adv vector using 5’and 3’ E-Syt3 primers (Table 1) ...
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bioRxiv - Cell Biology 2020Quote: ... of human ACLY was PCR amplified from a HeLa cDNA pool and inserted into the EcoRI-digested pLVX-puro vector (Takara) using the In-Fusion cloning system (Takara) ...
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bioRxiv - Molecular Biology 2021Quote: ... cDNAs were PCR-re-amplified and finally subcloned into Hind III site of the pEYFP-C1 or pECFP-C1 vectors (Clontech).
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bioRxiv - Molecular Biology 2021Quote: ... qRT-PCR was performed using SYBR Premix Ex TaqII (Tli RNaseH Plus) and the Thermal Cycler Dice Real Time System (TaKaRa). The results were analyzed based on standard curves from a serial dilution of cDNA ...
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bioRxiv - Molecular Biology 2021Quote: A DNA fragment encoding the full-length LARP1 gene (NM_033551) was PCR- amplified from HEK293T genomic DNA and inserted into the pColdI vector (TaKaRa, 3361) at the NdeI and HindIII sites ...
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bioRxiv - Immunology 2021Quote: ... Initial PCR amplification and the subsequent barcoding PCR reaction of the 3BNC117 VH fragments or the Off Target sites was performed using the proofreading PrimeStarMAX Polymerase (Takara) for 35 cycles and 8 cycles ...
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bioRxiv - Cell Biology 2021Quote: ... an additional set of genes encoding pTF.CREG1 with N-terminal truncations (Δ26, Δ31, Δ39, Δ43) was generated by PCR using PrimeStar GXL DNA Polymerase (Takara Bio), primer sets JT33/JT32 ...
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bioRxiv - Cell Biology 2020Quote: ... was amplified by PCR and inserted into NheI and AgeI sites of the mCherry-C1 and mCerulean3-C1 vectors (Clontech). YFP-PH(Akt ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The relative expression of genes was quantified by quantitative real-time polymerase chain reaction (qRT-PCR) analysis using SYBR Green Supermix (TaKaRa); β-actin served as a housekeeping gene ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Real-time reverse transcription quantitative PCR (qPCR) was performed in duplicate using 1 μL of cDNA from each sample and SYBR Premix Ex Taq (Takara) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2021Quote: ... The fragment length of PCR products was detected by 1.2% agarose gel electrophoresis using 5 μl of 100 bp DNA Ladder (TaKaRa, Japan) and gel stain (TransGen Biotech ...
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bioRxiv - Genetics 2021Quote: ... The composition of the reaction mixture for the PCR was as follows: 10 μl Premix Taq (Ex Taq Version 2.0 plus dye, TaKaRa, Japan), 0.3 μl each of forward and reverse primer (10μM) ...
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bioRxiv - Biochemistry 2021Quote: ... hCLPTM1L-FLAG-6His was amplified by PCR and cloned into pPB-tetON-BSD-tightFF plasmid by In-fusion cloning (Clontech). To construct pPB-tetON-Tmem16k-FLAG-6His-BSD-tightFF plasmid ...
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bioRxiv - Molecular Biology 2021Quote: ... the region from the start of eBFP1.2 until the end of iGFP was PCR-amplified from the modified pDRGFP and ligated into XbaI/MluI digested pLVX-IRES-Hyg (Clontech; this procedure removes the IRES-Hygro sequence) ...
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bioRxiv - Cell Biology 2021Quote: ... Borr open reading frame corresponding to amino acids 113-221 was first amplified with a stop codon from LD36125 by PCR using PrimeStar (Takara) and cloned between AscI and NotI sites of pENTR (ThermoFisher ...
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bioRxiv - Evolutionary Biology 2021Quote: ... respectively) and an mCherry coding sequence were amplified from the template plasmid by PCR using PrimeSTAR GLX DNA polymerase (TAKARA) along with the sense and antisense primer pair (5’-ATTACCTGGGGGTATCCCCTT-3’ and 5’-CACTCTTCTGGTTGTGGTTGC-3’) ...
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bioRxiv - Pathology 2021Quote: ... 5 µL of 1:10 diluted cDNA samples was used as the qRT-PCR template with 0.5 µM gene-specific primers and 10 µL SYBR Premix Ex Taq II (Takara, China) in a total volume of 20 µL ...