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Citations for Takara Bio :
1951 - 2000 of 2499 citations for PCR Strips since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2020Quote: ... and quantitative real time PCR was performed following manufacturer’s instructions given in SYBR1 Premix ExTaq (TliRNAse H Plus) (Clontech,USA). The PCR amplification was carried out in Rotor-Gene-Q Real-Time PCR System (Qiagen ...
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bioRxiv - Synthetic Biology 2020Quote: ... Homology arms were assembled into digested (SacI, XbaI) or PCR-amplified pLO3 backbone via Gibson Assembly (HiFi, NEB or In-fusion, Takara). C ...
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bioRxiv - Cell Biology 2019Quote: ... TRPV4(ΔPR)–myc and TRPV4(ΔAR1-3)–myc were constructed using PCR with template plasmids from Open Biosystems subcloned into pCMV-myc vector (Clontech) at the SalI/XhoI sites ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: 5’ and 3’ cDNA ends were subsequently amplified by RACE PCR using the SMARTer RACE cDNA Amplification Kit and Advantage HF2 DNA polymerase (Clontech) with gene-specific primers derived from the initial RT-PCR product (Supplemental Information ...
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bioRxiv - Cell Biology 2020Quote: ... Mouse E-Syt3 cDNA was amplified by PCR from a cDNA library constructed with poly(A) RNA from 3T3-L1 adipocytes (Mate & Plate TM Library System, Clontech) and cloned into the PT-Adv vector using 5’and 3’ E-Syt3 primers (Table 1) ...
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bioRxiv - Genetics 2021Quote: ... This mutated fragment was replaced by the original homologous sequence of the pJet-ebony_mut-mCherry vector by fusing the mutated fragment and the PCR product amplified from the pJet-ebony-mCherry vector using the In-Fusion HD Cloning Kit (TaKaRa) with the following primers ...
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bioRxiv - Genetics 2021Quote: ... Reverse transcription was then performed using the oligo (dT) primer and Avian myeloblastosis virus reverse transcriptase contained in the TaKaRa RNA PCR kit (TaKaRa). We conducted RT-PCR using KOD One (TOYOBO ...
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bioRxiv - Cell Biology 2020Quote: ... of human ACLY was PCR amplified from a HeLa cDNA pool and inserted into the EcoRI-digested pLVX-puro vector (Takara) using the In-Fusion cloning system (Takara) ...
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bioRxiv - Developmental Biology 2019Quote: ... The cDNA was diluted 1:1 with nuclease free water and subjected to qRT-PCR using SyBr green mix (Takara) in real-time PCR machine (BioRad CFX96) ...
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bioRxiv - Molecular Biology 2021Quote: ... cDNAs were PCR-re-amplified and finally subcloned into Hind III site of the pEYFP-C1 or pECFP-C1 vectors (Clontech).
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bioRxiv - Cancer Biology 2019Quote: ... 20-50ng of the first PCR amplicon in a total PCR reaction volume of 25ul was amplified in a 12 cycle PCR using primer set 2 and PrimeSTAR GXL DNA Polymerase (Takara) (Supplemental Table 1b) ...
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bioRxiv - Molecular Biology 2021Quote: ... qRT-PCR was performed using SYBR Premix Ex TaqII (Tli RNaseH Plus) and the Thermal Cycler Dice Real Time System (TaKaRa). The results were analyzed based on standard curves from a serial dilution of cDNA ...
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bioRxiv - Molecular Biology 2021Quote: A DNA fragment encoding the full-length LARP1 gene (NM_033551) was PCR- amplified from HEK293T genomic DNA and inserted into the pColdI vector (TaKaRa, 3361) at the NdeI and HindIII sites ...
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bioRxiv - Microbiology 2020Quote: ... according to the manufacturer’s instructions and detected by RT-qPCR assays with a One-Step PrimeScript RT-PCR kit (Takara, Japan) using SARS-CoV-2-specific primers on an Applied Biosystems 7500 Real-time PCR System.
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bioRxiv - Cell Biology 2020Quote: ... according to the manufacturer’s protocol.Forty ng cDNA was used as templates for RT-PCR using SYBR® Premix Ex Taq kit (TaKaRa,#RR820A) using LightCyclerW 480 (Roche ...
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bioRxiv - Immunology 2021Quote: ... Initial PCR amplification and the subsequent barcoding PCR reaction of the 3BNC117 VH fragments or the Off Target sites was performed using the proofreading PrimeStarMAX Polymerase (Takara) for 35 cycles and 8 cycles ...
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bioRxiv - Developmental Biology 2019Quote: ... 10 ng total RNA was reverse transcribed and full-length cDNA was specifically amplified by 8 PCR cycles using SMART-Seq v4 Ultra Low Input RNA Kit (Clontech) (31) ...
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bioRxiv - Biochemistry 2019Quote: ... A human Podocin-N fragment cDNA with 3xFLAG was amplified by PCR using a human kidney cDNA in Human MTC Panel I (TaKaRa) as a template ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Forward (control) and reverse (target) RNA probes were synthesized from plasmids amplified via PCR (Advantage HD Polymerase Kit, Takara Bio) using T7 or SP6 RNA Polymerases (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2019Quote: ... RNA with an OD value (A260/A280) of 1.8–2.0 was reversed transcribed to cDNA by using a PrimeScriptTM RT-PCR kit (Takara, Kusatsu, Japan). cDNA was amplified by Takara Taq™ (DR001AM ...
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bioRxiv - Cell Biology 2021Quote: ... Viral RNA was quantified using a One Step TB Green PrimeScript PLUS RT-PCR Kit (Perfect Real Time) (Takara Bio) on a StepOnePlus real-time PCR system (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2021Quote: ... an additional set of genes encoding pTF.CREG1 with N-terminal truncations (Δ26, Δ31, Δ39, Δ43) was generated by PCR using PrimeStar GXL DNA Polymerase (Takara Bio), primer sets JT33/JT32 ...
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bioRxiv - Cell Biology 2020Quote: ... was amplified by PCR and inserted into NheI and AgeI sites of the mCherry-C1 and mCerulean3-C1 vectors (Clontech). YFP-PH(Akt ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The relative expression of genes was quantified by quantitative real-time polymerase chain reaction (qRT-PCR) analysis using SYBR Green Supermix (TaKaRa); β-actin served as a housekeeping gene ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Real-time reverse transcription quantitative PCR (qPCR) was performed in duplicate using 1 μL of cDNA from each sample and SYBR Premix Ex Taq (Takara) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2021Quote: ... The fragment length of PCR products was detected by 1.2% agarose gel electrophoresis using 5 μl of 100 bp DNA Ladder (TaKaRa, Japan) and gel stain (TransGen Biotech ...
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bioRxiv - Genetics 2021Quote: ... The composition of the reaction mixture for the PCR was as follows: 10 μl Premix Taq (Ex Taq Version 2.0 plus dye, TaKaRa, Japan), 0.3 μl each of forward and reverse primer (10μM) ...
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bioRxiv - Biochemistry 2021Quote: ... hCLPTM1L-FLAG-6His was amplified by PCR and cloned into pPB-tetON-BSD-tightFF plasmid by In-fusion cloning (Clontech). To construct pPB-tetON-Tmem16k-FLAG-6His-BSD-tightFF plasmid ...
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bioRxiv - Molecular Biology 2021Quote: ... the region from the start of eBFP1.2 until the end of iGFP was PCR-amplified from the modified pDRGFP and ligated into XbaI/MluI digested pLVX-IRES-Hyg (Clontech; this procedure removes the IRES-Hygro sequence) ...
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bioRxiv - Developmental Biology 2021Quote: ... cDNA synthesis from the total RNA was carried out using the PrimeScriptTM High Fidelity RT-PCR Kit (TaKaRa, Shiga, Japan) using an oligo dT primer ...
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bioRxiv - Cell Biology 2021Quote: ... Borr open reading frame corresponding to amino acids 113-221 was first amplified with a stop codon from LD36125 by PCR using PrimeStar (Takara) and cloned between AscI and NotI sites of pENTR (ThermoFisher ...
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bioRxiv - Evolutionary Biology 2021Quote: ... respectively) and an mCherry coding sequence were amplified from the template plasmid by PCR using PrimeSTAR GLX DNA polymerase (TAKARA) along with the sense and antisense primer pair (5’-ATTACCTGGGGGTATCCCCTT-3’ and 5’-CACTCTTCTGGTTGTGGTTGC-3’) ...
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bioRxiv - Pathology 2021Quote: ... 5 µL of 1:10 diluted cDNA samples was used as the qRT-PCR template with 0.5 µM gene-specific primers and 10 µL SYBR Premix Ex Taq II (Takara, China) in a total volume of 20 µL ...
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bioRxiv - Biophysics 2020Quote: ... Mutations with multiple amino acids were introduced by ligating inverse PCR-amplified backbone with mutations bearing DNA oligonucleotides via the In-Fusion Cloning Kit (ClonTech). All mutants were confirmed by Sanger sequencing.
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bioRxiv - Cell Biology 2021Quote: ... One microgramme of total RNA was reverse-transcribed using a One Step PrimeScript™ RT-PCR Kit (Takara, Liaoning, China) with a thermocycler ...
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African Swine Fever Virus CD2v protein promotes β-Interferon expression and apoptosis in swine cellsbioRxiv - Microbiology 2020Quote: ... ORFV120 and ORFV113 coding sequences were PCR-amplified from orf virus strain OV-IA82 genome and cloned into p3xFlag-CMV-10 vector (pFlag) (Clontech).
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bioRxiv - Microbiology 2020Quote: ... The EGFP-nsP3 truncations and alanine substitution mutations were constructed by subcloning the corresponding nsP3-encoding PCR products into the vector pEGFP-C1 (Clontech) using XhoI-KpnI restriction sites.
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bioRxiv - Microbiology 2020Quote: ... Cells were originally obtained from ATCC (except MAGIC5 cells) and routinely tested negative for mycoplasma contamination (PCR Mycoplasma Detection kit, Takara).
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bioRxiv - Microbiology 2020Quote: ... The infectious titer of lentivirus was determined by a Lenti-X™ qRT-PCR Titration Kit (Clontech, Mountain View, CA).
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bioRxiv - Molecular Biology 2021Quote: ... Mutations with multiple amino acids were introduced by ligating inverse PCR-amplified backbone with mutations bearing DNA oligonucleotides via the In-Fusion Cloning Kit (ClonTech). All mutants were confirmed by Sanger sequencing.
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bioRxiv - Immunology 2021Quote: Variable heavy chain and light chain sequences were PCR amplified from yeast plasmid DNA and cloned into CMV/R IgG expression vectors using InFusion (Takara). Plasmid DNA for mammalian expression of the His-tagged SARS-CoV-2 RBD in a pCAGGS vector was kindly provided by Dr ...
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bioRxiv - Microbiology 2021Quote: ... three inserts were amplified by PCR and sequentially inserted in two steps using the In-Fusion HD Cloning Kit (Clontech). In the first step ...
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bioRxiv - Immunology 2020Quote: ... This construct was cloned out of the parent vector and into an in-house pADD2 vector using HiFi PCR (Takara) followed by In-Fusion (Takara ...
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bioRxiv - Microbiology 2021Quote: Expression plasmids encoding for human ZMPSTE24 with and without a C-terminal FLAG-tag or HA-tag were PCR amplified and subcloned into the pQXCIP (Clontech) backbone using flanking restriction sites AgeI and BamHI ...
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bioRxiv - Genetics 2019Quote: ... the genes of interest were PCR amplified adding SfiI restriction sites and ligated to modified version of the vectors pGBKT7 and pGADT7 (Clontech) containing SfiI restriction sites.
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bioRxiv - Cell Biology 2021Quote: ... mCherry-STIM1-10A and WT STIM1 were constructed by inserting PCR fragments into XhoI-HpaI sites of pMSCV-puro vector (Clontech) and were packaged using the Phoenix-ECO cell line (ATCC CRL-3214) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The concentrations of the host and the parasitic RNAs were measured by RT-qPCR (PrimeScript One Step RT-PCR Kit (TaKaRa)) with sequence-specific primers (Supplementary text).
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bioRxiv - Genetics 2020Quote: ... The products obtained were subsequently subjected to semi-nested PCR with a LA Taq Hot Start polymerase kit (TaKaRa Biotechnology). The first round of amplification was performed with primers ...
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bioRxiv - Neuroscience 2020Quote: ... qRT-PCR was performed using TOYOBO THUNDERBIRD SYBR qPCR Mix on a Thermal Cycler Dice Real Time System (Takara Bio). The average threshold cycle value (CT ...
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bioRxiv - Microbiology 2020Quote: ... which corresponds to the S23Q/L24M mutant of SARS-CoV-2 Wuhan-Hu-1 ORF3b *57) was generated by overlap extension PCR by using PrimeSTAR GXL DNA polymerase (Takara), the SARS-CoV-2 ORF3b 155* as the template ...