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Citations for Takara Bio :
1851 - 1900 of 2499 citations for PCR Strips since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: Nested translocation PCR assays was performed from genomic DNA as previously described (29) using either LA Taq DNA polymerase (Takara) or Flex HS polymerase (NEB) ...
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bioRxiv - Cell Biology 2022Quote: ... LC NLS deletion (Δ417-422) was generated by KOD One PCR amplification and the In-Fusion HD Cloning Kit (Clontech). The NLS of SUN2 (KDSPLRTLKRKSSNMKRL ...
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bioRxiv - Genomics 2020Quote: ... 10 μL of which was subject to PCR with primer pair 5′- AATGATACGGCGACCACCGAGATCT-ACACTCTTTCCCTACACGACGCTCTTCCGATCT-3′ and 5′-CAAGCAGAAGACGGCATACGAGAT-CTGATC-TGACTGGAGTTCAGACGTGTGCTCTTCCGATCT-GCTGCGCTCGATGCAAAATA-3′ using PrimeSTAR Max PCR master mix (R045A, Takara) to add sequencing adapter sequences to CASB barcode.
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bioRxiv - Molecular Biology 2021Quote: ... The first stranded cDNA was next amplified to produce double stranded cDNA in 20 amplification cycles by long distance PCR using the Advantage 2 polymerase mix (Takara, Clontech) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... followed by reverse transcription of the enriched mRNA into cDNA using Clontech SMARTer PCR cDNA Synthesis Kit (Takara, Shiga, Japan). PCR cycle optimization was used to determine the optimal amplification cycle number for the downstream large-scale PCR reactions ...
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bioRxiv - Cancer Biology 2019Quote: ... we performed 30 separate 100 μL PCR reactions with 10 μg genomic DNA in each reaction using ExTaq DNA Polymerase (Clontech) then combined the resultant amplicons ...
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bioRxiv - Cell Biology 2019Quote: The GFP-TAZ and GFP-YAP constructs were generated by PCR and sub-cloned into the pEGFP-C1 vector (Clontech) or pGFP-2xStrep vector ...
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bioRxiv - Plant Biology 2019Quote: ... The qRT-PCR was performed by the instruction of SYBR Premix Ex Taq (Tli RNaseH Plus; Takara Biotechnology, Dalian, China). The reaction condition was ...
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bioRxiv - Developmental Biology 2019Quote: ... The expression of each gene was determined by q-PCR using the SYBR Premix Ex Taq (RR402A; Takara Bio. Inc.), and β-actin was used as the reference gene ...
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bioRxiv - Biochemistry 2019Quote: ... 5’UTRs of MAGED2 TV2 and TV3 were amplified by RT-PCR from RNA isolated from human testes tissue purchased from Takara Bio (Mountain View ...
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bioRxiv - Microbiology 2019Quote: ... These PCR products were cloned into pCA24N with a C-terminal GFP tag using the In-Fusion HD enzyme kit (Takara). Clones were selected on TSA plates with 50µg/ml chloramphenicol ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The PCR products were cloned into pBAD28 vector (ATCC® 87400™) by using In-Fusion HD Cloning kit (Takara) by using primers 7 and 8 to amplify pBAD28 vector (Table S2 ...
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bioRxiv - Plant Biology 2019Quote: ... The qRT-PCR amplification was run in an ABI 7500 HT (Life technology) with SYBR Green I Master Mix (TaKaRa), and the reaction mixture and program was carried out as described in our previous work (Wu et al. ...
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bioRxiv - Developmental Biology 2019Quote: ... Antisense probes for ccna1/ ccna2/ ccne1/ top2a were made using the PCR products as templates for RNA synthesis with T7 RNA polymerases (Takara). The stained embryos were dehydrated in glycerol and photographed with a Nikon SMZ1500 stereomicroscope (Nikon ...
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bioRxiv - Cancer Biology 2019Quote: ... The resultant cDNA was subjected to real-time PCR by using SYBR® Premix Ex Taq™ II (Takara Bio) and specific primer sets (Takara Bio) ...
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bioRxiv - Synthetic Biology 2019Quote: ... the iLID-encoding sequence was PCR-amplified from Venus-iLID-CAAX and inserted into CRY2PHR-mCh-iTrkA7 at XhoI and SmaI using In-Fusion (Clontech). iLID-mRuby3-iTrkB was generated by PCR amplification of iLiD ...
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bioRxiv - Microbiology 2019Quote: ... pcDNA-TCRα was digested with EcoRI and incubated with purified PCR product amplified with the PrimeStar DNA polymerase (Takara – Ozyme) for 3 min at RT followed by 10 min on ice ...
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bioRxiv - Microbiology 2019Quote: ... A plasmid encoding a gene fusion between the 5‘-201 nucleotides of PR8 segment 5 and GFP was made by PCR-cloning the appropriate IAV sequence into pEGFP-1 (Clontech), followed by oligonucleotide-directed PCR mutagenesis (using standard protocols ...
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bioRxiv - Pathology 2021Quote: ... Viral titering was performed using 15 μL of unconcentrated virus or 1.5 μl of concentrated virus with the Lenti-X qRT-PCR Titration Kit (Clontech, 632165). Results were read on an ABI QuantStudio 6 RT-PCR machine.
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bioRxiv - Genetics 2020Quote: ... Human CAD was PCR amplified from cDNA (Open Biosystems clone ID 5551082) using specific primers (Table S1) and ligated with In-Fusion (Clontech) into NotI linearized pcDNA3.1-GFP ...
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bioRxiv - Genomics 2021Quote: ... Target regions were amplified by PCR using specific primer sets and high-fidelity PrimeSTAR GXL DNA polymerase (Takara, Shiga, Japan). Sanger sequencing was performed using BigDye Terminator reactions and loaded onto a 3730xl DNA analyzer (Applied Biosystems ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Genes of target prokaryotic proteins were amplified by PCR from corresponding genomic DNA and inserted into the pEGFP-C1 vector (Clontech). Mutated genes of prokaryotic proteins were obtained by PCR site-specific mutagenesis ...
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The Ets protein Pointed P1 represses Asense expression in type II neuroblasts by activating TaillessbioRxiv - Developmental Biology 2021Quote: ... Tll or PntP1 coding region was amplified using the CloneAmp HiFi PCR Premix (Catalog# 639298, Takara Bio., Mountain View, CA) from a cDNA library and cloned into pcDNA™3.1/His expression vectors (Catalog #V38520 ...
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bioRxiv - Developmental Biology 2021Quote: All novel plasmids constructed for this study were based on the pSP Sox1/2/3> plasmid previously described (9) with the open reading frames replaced by PCR amplifications using a proofreading DNA polymerase (Primestar, Takara) and plasmids were assembled from linear PCR products using NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs) ...
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bioRxiv - Plant Biology 2020Quote: ... Quantitative PCR was performed with the ABI PRISM Fast 7500 Real-Time PCR engine using the TB Green Premix Ex TaqII (TIi RNaseH Plus) (TaKaRa) and the miRcute Plus miRNA qPCR kit (SYBR Green ...
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bioRxiv - Neuroscience 2021Quote: ... cytosol and/or nucleus content in each pipette were expelled into individual PCR tubes containing 11.5 µl of lysis buffer (Takara, 634984) and stored in −80 °C.
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bioRxiv - Cell Biology 2021Quote: ... The vimentin-null mEFs expressing vimentin are created by PCR amplification of the vimentin coding sequence using CloneAmp polymerase (Clontech) from pcDNA4-vimentin (provided by J ...
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bioRxiv - Bioengineering 2020Quote: ... 500 ng of total RNA was reverse transcribed (complementary DNA (cDNA) was synthesized using PrimeScript™ RT-PCR Kit (Takara) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... Lentivirus was concentrated from supernatant using ultracentrifugation and the genome copy number was calculated using the Clontech Lenti-X qRT-PCR Titration kit (Takara).
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bioRxiv - Biochemistry 2020Quote: ... The PCR product was cloned into pET28a digested with restriction enzymes NotI and NdeI using InFusion HD Cloning (Takara Bio). Both cloning strategies introduce a hexahistidine tag followed by a thrombin cleavage sequence ...
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bioRxiv - Biophysics 2021Quote: ... denatured at 85°C for 5 minutes and annealed at 47.5°C for 4 hours in a PCR machine (Takara-Bio). The folded DNA origami was then agarose-gel-purified (Douglas et al ...
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bioRxiv - Cell Biology 2021Quote: ... dual sgRNA cassettes and plasmid DNA (pDNA) were PCR-amplified and barcoded with sequencing adaptors using ExTaq DNA Polymerase (Clontech) following the same procedure as Supplementary Note 1 in Najm et al ...
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bioRxiv - Cell Biology 2021Quote: ... BicD and GFP CDS were amplified by PCR and the two fragments were combined into AttB-pUASp-K10 vector by InFusion cloning (Clontech) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Real-time qRT-PCR was performed as previously described using TB Green Premix Ex Taq II (Takara Bio Inc, Japan) and a QuantStudio 7 Real-Time PCR system (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: Cultured rat cortical neurons were infected with recombinant lentiviruses at DIV3 and harvested at DIV10 for qRT-PCR using SYBR green qPCR master mix (Takara). Total RNA was extracted from rat cortical neurons using the TRIzol reagent (Invitrogen ...
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bioRxiv - Cell Biology 2020Quote: ... The plasmid pJYB240 was generated from pJYB212 by exchanging the mEos2 open reading frame by a PCR-amplified mTurquoise2 DNA (Goedhart et al. 2012) using the InFusion kit (Clontech). The parBF-mTurquoise2 gene from pJYB240 was introduced into pJYB243 ...
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bioRxiv - Microbiology 2020Quote: ... The resulting PCR products were cloned into a NsilI/SphI digested pSW-lacZ::sfGFP backbone by infusion cloning (Takara, Z9633N).
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bioRxiv - Neuroscience 2020Quote: Cultured rat cortical neurons were infected with recombinant lentiviruses at DIV4 and harvested at DIV11 for qRT-PCR using SYBR green qPCR master mix (TaKaRa). Total RNA was extracted from mouse cortical neurons using TRIzol reagent (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... The viral load was measured by RT-qPCR using One-Step SYBR® Primescript™ RT-PCR kit II (Takara). CT values of serum samples were used to calculate serum viral titre according to regression equation built by RNA extracted from 10 µL of 102-106 pfu/mL of ZIKV (PRVABC59 or MP1751) ...
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bioRxiv - Microbiology 2021Quote: ... and the resulting PCR product gel extracted and inserted into the XhoI/NheI-cut plasmid via In-Fusion Cloning (Takara) to make the donor vector pM2GT-1437000-mNeonGreen-3xHA ...
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bioRxiv - Neuroscience 2020Quote: ... The virus titer of purified AAV stocks was determined by AAVpro® Titration kit (for Real Time PCR) ver2 (TaKaRa).
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bioRxiv - Neuroscience 2020Quote: ... the 11kb genomic region encompassing kdm5 was amplified by PCR from w1118 and cloned into the pattB vector (Bischof et al., 2007) using the In-Fusion cloning system (Takara). A 3xHA tag was included in-frame at the 3’end of the kdm5 ORF ...
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bioRxiv - Immunology 2021Quote: ... [50]) coding for Drosophila SPARC was used as a template for PCR amplification using Takara Ex Taq polymerase (Takara Biomedicals) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... mIRE1-wt was PCR-amplified (without a myc tag) from pcDNA3-mIRE1-3xmyc (Stahl et al, 2013) and subcloned in pMSCVhyg (Clontech) using BglII and HpaI sites ...
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bioRxiv - Microbiology 2021Quote: ... Two ∼0.5□kb DNA fragments flanking the genes of interest were amplified by PCR using PrimeSTAR GXL DNA polymerase (Takara bio), M ...
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bioRxiv - Neuroscience 2020Quote: ... which was performed according to the manufacturer’s guidelines using the iCell8 Chip and Reagent Kit using in-chip RT-PCR amplification chemistry (Wafergen, Takara Bio) and Nextera XT DNA Library Preparation Kit and Nextera XT Index Kit (Illumina) ...
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bioRxiv - Cell Biology 2020Quote: ... 3C protease-cleavage site and a His12-tag at the N-terminus were amplified by PCR using KOD-Plus-Neo polymerase (Toyobo) and Human Universal QUICK-Clone cDNA II (Clontech) as a template cDNA and then cloned into a pET-41 Ek/LIC vector (Novagen) ...
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bioRxiv - Plant Biology 2021Quote: ... captured DNA fragments were PCR-amplified using Takara LA Taq DNA Polymerase over 15 cycles (Clontech/Takara Bio, Otsu, Japan) then purified using AMPure PB Beads (Beckman Coulter ...
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bioRxiv - Microbiology 2019Quote: ... The resulting product was purified from a 1% agarose gel using the NucleoSpin Gel and PCR Clean-up Kit (Takara) and Sanger sequenced.
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bioRxiv - Microbiology 2019Quote: ... PCR was conducted with tprK-specific primers (S1 Table) appended to 16bp PacBio barcodes using CloneAmp HiFi polymerase mix (Takara) and the following conditions ...