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Citations for Takara Bio :
151 - 200 of 1523 citations for pLenti CMV TO Puro DEST 670 1 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... The amplified DNAs were subcloned into the pCK-FLAG vector (CMV promoter-driven vector) at the BamHI and XhoI sites using In-Fusion HD Cloning Kit (Clontech). For dsRBD-deleted DICER ...
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bioRxiv - Cell Biology 2021Quote: ... Expression from the CMV-TetO2 promoter in Flp-In T-REx cells was induced by treatment with 10 ng/mL doxycycline (Clontech) for 24 hours ...
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African Swine Fever Virus CD2v protein promotes β-Interferon expression and apoptosis in swine cellsbioRxiv - Microbiology 2020Quote: ... ORFV120 and ORFV113 coding sequences were PCR-amplified from orf virus strain OV-IA82 genome and cloned into p3xFlag-CMV-10 vector (pFlag) (Clontech).
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bioRxiv - Immunology 2021Quote: Variable heavy chain and light chain sequences were PCR amplified from yeast plasmid DNA and cloned into CMV/R IgG expression vectors using InFusion (Takara). Plasmid DNA for mammalian expression of the His-tagged SARS-CoV-2 RBD in a pCAGGS vector was kindly provided by Dr ...
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bioRxiv - Neuroscience 2021Quote: ... The PGK promoter-puromycin resistance gene cassette in the vector was further replaced by the CMV-eGFP cassette from the pEGFP-C1 vector (Clontech). The sequence-verified plasmid was transfected into Lenti-X 293T cells (Takara Bio ...
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bioRxiv - Synthetic Biology 2023Quote: ... each for a 50-µL reaction containing ∼30 fmol of EcoRI-XbaI digested pLVSIN-CMV-Pur backbone plasmid (Takara #6183), ∼300 fmol of the insert fragment ...
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bioRxiv - Neuroscience 2022Quote: ... These guides were individually cloned into pAAV-U6-sasgRNA-CMV-mCherry-WPREpA at the BstXI and XhoI restriction enzyme sites using the In-Fusion HD cloning kit (Clontech). rAAV vectors were generated using similar plasmids and cloning methods as was referenced in (Matharu et al. ...
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bioRxiv - Molecular Biology 2022Quote: ... Mammalian expression vector for Flag-EBNA1 contained B95-8 EBNA1 lacking the GA repeats (aa 101-324) under the control of CMV-3XFLAG promoter in a plasmid derived from pREP10 (Clontech) containing ...
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bioRxiv - Neuroscience 2023Quote: Mammalian expression constructs were mainly generated by Gibson assembly of PCR-amplified coding sequences (primers, Supp. Data File 2) into restriction-digested pC1 (CMV promoter, modified from pEGFP-C1, Clontech) or pCAG (CMV enhancer fused to chicken beta-actin promoter ...
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bioRxiv - Immunology 2024Quote: An adenovirus plasmid encoding the first 30 amino acids of KRAS with the G12V mutation (KRAS G12V-30) was created following the manufacturer’s instructions (Adeno-X CMV, Takara Bio). Adenovirus particles were produced by transfecting the adenoviral plasmid into HEK293 cells ...
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bioRxiv - Biochemistry 2024Quote: ... an attB recombination site was inserted in place of the CMV promoter by InFusion HD Cloning (Takara Bio, Shiga, Japan). To facilitate the creation of these libraries using nicking mutagenesis ...
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bioRxiv - Cell Biology 2022Quote: ... All above sequences along with MBNL1 and MBNL2 isoforms imaged in primary neurons were cloned downstream of GFP in a pEGFP-C1 plasmid backbone under control of a CMV promoter (Takara). Full-length kinesin sequences were amplified from mouse cDNA and inserted upstream of an mScarlet-containing plasmid in a pcDNA3.1 backbone under the control of CMV promoter.
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bioRxiv - Immunology 2022Quote: ... PGDM1400 heavy and light chain sequences were synthesized (Integrated DNA Technologies) and cloned into a mammalian expression vector under a CMV promoter using InFusion (Takara) and sequence verified.
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bioRxiv - Genetics 2024Quote: ... Esrp1 cDNA was cloned into the pcDNA3.1 backbone containing a CMV promoter and SV40 polyA tailing sequence for expression in mammalian cells using the In-Fusion HD Cloning Kit (Clontech) to generate the pcDNA3.1-esrp1-mCherry plasmid ...
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bioRxiv - Cell Biology 2024Quote: ... CMV-GFP-PLDPMF and PGK-GFP-p85iSH2 were constructed by inserting the coding sequence for each gene into the CMV-EGFP-C1 expression vector (Clontech) and PGK-EGFP-C1 plasmid respectively via HindIII and KpnI.
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bioRxiv - Genetics 2021Quote: ... pPB_U6::gRNA_EF1a::BFP-Puro containing a gRNA against Esg1 and pPY_CAG_Pbase using Xfect mESC transfection reagent (Takara #631320) and selection (Neomycin (300 μg/ml ...
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bioRxiv - Cell Biology 2022Quote: ... followed by subsequent cloning of the GFP-TMEM11 cassette into the Xho/BamHI sites of pLVX-Puro (Takara) by isothermal assembly ...
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bioRxiv - Cancer Biology 2021Quote: ... the cDNA sequence of ERK5 isoforms were cloned into pLVX-Puro plasmid (Clontech Laboratories, Mountain View, CA, USA). Plasmids were sequenced to confirm the presence of correct cDNA sequences at the 3’-end of the CMV promoter (data not shown) ...
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bioRxiv - Molecular Biology 2023Quote: The lentiviral backbone construct was designed starting from the commercial pLVX-EF1α-IRES-Puro vector (Takara Bio, 631253), to which a T2A-mTagBFP2 cassette (Twist Bioscience ...
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bioRxiv - Neuroscience 2023Quote: EGFP-tagged rat PKCδ cDNA were cloned into EcoRI and BamHI digested pRetroX-TetOne-Puro (#634307, Takara Bio) using In-Fusion cloning system (#639648 ...
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bioRxiv - Immunology 2024Quote: ... STAT1 cDNA was PCR-amplified from Addgene #8691 (a gift from Jim Darnell (Horvath et al, 1995)) and sub-cloned into pLVX-IRES-Puro (Takara) via the BamHI and NotI sites ...
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bioRxiv - Cell Biology 2024Quote: 2xRFP-NLS was generated by inserting mCherry-NLS-tagRFP (G-Block, IDT) into pLVXEblast (pLVXE-IRES-puro (Clontech) with puroR replaced by blastR ...
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bioRxiv - Neuroscience 2021Quote: ... These were then packaged into lentivirus via cotransfection with CMV-Gag-Pol (Harvard #dR8.91) and pVSV-G (Clontech, part of #631530) plasmids into production HEK293T cells adapted to grow in serum-free conditions (OHN media ...
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bioRxiv - Synthetic Biology 2022Quote: ... VH and VL segments were ordered as gene blocks from Integrated DNA Technologies and were cloned into linearized CMV/R backbones with 5X In-Fusion HD enzyme premix (Takara Bio).
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bioRxiv - Immunology 2022Quote: ... (both kind gifts from Pilar Gonzalo and Joaquin Teixidó (Bartolomé et al. 2009)) were cloned into the lentiviral backbone pLVX-CMV-IRES-zsGreen1 (Takara/Clontech #632187) by Gibson assembly (Gibson et al ...
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bioRxiv - Synthetic Biology 2023Quote: ... VH and VL segments were ordered as gene blocks from Integrated DNA Technologies and were cloned into linearized CMV/R backbones with 5× In-Fusion HD Enzyme Premix (Takara Bio).
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bioRxiv - Microbiology 2023Quote: ... This clone was digested with NsiI and BglII to remove the CMV promoter sequence and was combined with the pSV010(-) SV40 promoter fragment using the Infusion® HD Cloning kit (Clontech) such that the pSV010(- ...
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bioRxiv - Immunology 2024Quote: ... These fragments were then cloned into the CMV/R plasmid containing the VRC01 HC and LC constant domains by In-Fusion (Takara Bio). DNA encoding the wild-type SARS-CoV-2 Wuhan Hu-1 receptor-binding domain (residues 319-533 ...
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bioRxiv - Microbiology 2020Quote: The VZV thymidine kinase gene (ORF36) was inserted into the Lenti-X pLVX-Puro vector (Clontech, Mountain View, CA) with XhoI and EcoR I sites ...
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bioRxiv - Biochemistry 2022Quote: Expression inserts for cell lines were generated by PCR amplification and ligated into the pLVX-tight-Puro vector (Clontech) using the NEB HiFi DNA Assembly Kit (New England Biolabs ...
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bioRxiv - Microbiology 2022Quote: The plasmids of the SARS-CoV-2 cDNA library as cloned in the pLVX-EF1alpha-IRES-Puro (Takara/Clontech) vector were a kind gift from Prof ...
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bioRxiv - Microbiology 2022Quote: The plasmids of the SARS-CoV-2 cDNA library as cloned in the pLVX-EF1alpha-IRES-Puro (Takara/Clontech) vector were a kind gift from Prof ...
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bioRxiv - Cancer Biology 2022Quote: ... The HAND2 mutant (HAND2mut) open reading frame was cloned into the doxycycline inducible pLVX-pTetOne-puro vector (Takara Bio) using In-Fusion HD (Takara Bio ...
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bioRxiv - Neuroscience 2021Quote: Auto-TDP-43-HA was then packaged into lentivirus (LV) via co-transfection with CMV-Gag-Pol (Harvard #dR8.91) and pVSV-G (Clontech, part of #631530) plasmids into production HEK293T cells adapted to grow in serum-free conditions (OHN media ...
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bioRxiv - Immunology 2022Quote: ... (both kind gifts from Pilar Gonzalo and Joaquin Teixidó (Bartolomé et al. 2009)) were cloned into the lentiviral backbone pLVX-CMV-IRES-zsGreen1 (Takara/Clontech #632187) by Gibson assembly (Gibson et al ...
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bioRxiv - Genomics 2022Quote: ... These guides were individually cloned into pAAV-U6-sasgRNA-CMV-mCherry-WPREpA (92) at the BstXI and XhoI restriction enzyme sites using the In-Fusion (Takara Bio, 638910) cloning methods as described in (92) ...
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bioRxiv - Cell Biology 2024Quote: ... The cDNA was subcloned into the pBApo-CMV pur expression vector at the XbaI and PstI sites (Takara Bio, San Jose, CA). V5 and 6-histidine tags were included on the carboxyl-terminal for protein detection and purification purposes25 ...
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bioRxiv - Molecular Biology 2021Quote: The Tet-on vector was obtained from the Lenti-X Tet-One Inducible Expression System (Puro) (Clontech, Cat. No. 634847). We transfected 1 × 106 HDR-immortalized cells using the Neon nucleofection system and 10 μg of plasmid DNA (plasmid encoding Cre recombinase ...
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bioRxiv - Microbiology 2022Quote: ... non-structural genes were PCR amplified in three fragments from the pRepDVRluc plasmid [37] and all fragments were inserted into the PUb-MCS-2A-Puro plasmid [64] using In-Fusion cloning kit (Takara) to generate the PUb-NS(Ø ...
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bioRxiv - Biochemistry 2020Quote: ... pSUPER-hygro was derived from the pSUPER-puro (Oligoengine) by replacing a puromycin resistance gene sequence with a hygromycin resistance gene sequence from pMSCV-hygro vector (Clontech), and it was used for expressing shRNA in NIH3T3 fibroblasts by retroviral infection ...
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bioRxiv - Cancer Biology 2020Quote: ... doxycycline-inducible ICAM-1 overexpressing vector was created through the cloning of full-length ICAM1 cDNA into a Tet-On lentiviral plasmid pLVX-Tight-Puro (632162, Clontech). The gene expressing vector and the regulator vector (pLVX-Tet-On Advanced ...
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bioRxiv - Cell Biology 2021Quote: Site-directed mutagenesis of pLenti-CMV-Neo-PINK1 (C125G)-EYFP or pLVX-puro-OMA1 (E328Q)-EYFP was performed by PCR amplification (CloneAmp HiFi PCR Premix, Takara or Q5 High-Fidelity DNA Polymerase system ...
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bioRxiv - Cancer Biology 2022Quote: ... in which pRetroX-TetOne puro empty vector or pRetroX-TetOne puro-ALKBH5 were each cotransfected with the envelope vector VSV-G using Xfect transfection reagent (Clontech). BEAS2B ...
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bioRxiv - Cancer Biology 2022Quote: ... Strbp and Upf1 (Table S1) were inserted between the BamHI and EcoRI sites of the knockdown vector pSirenRetroQ-puro (Clontech). The knockdown vectors for mouse Cebpb (Cebpb1 shRNA ...
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bioRxiv - Cell Biology 2020Quote: ... of human ACLY was PCR amplified from a HeLa cDNA pool and inserted into the EcoRI-digested pLVX-puro vector (Takara) using the In-Fusion cloning system (Takara) ...
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bioRxiv - Cell Biology 2020Quote: ... the sequence of NAGTI-GFP (N-acetylglucosaminyltransferase I fused to GFP; (Shima et al., 1997)) was cloned into pLVX-TetOne-Puro (Clontech). The constructed plasmid was then co-transfected with psPAX and pVSVG into HEK293T cells to produce lenti-viruses ...
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bioRxiv - Cell Biology 2020Quote: ... we first inserted the mAID sequence after 3xFlag in pCRISPaint-3xFlag-puro (Schmid-Burgk et al., 2016) using the In-Fusion HD Cloning kit (Takara) to generate pCRISPaint-3xFlag-mAID-puro ...
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bioRxiv - Genetics 2021Quote: ... full-length cDNA encoding MELK was inserted between BamHI and NotI sites of three types of retroviral plasmids including the pRetroX-Tight-puro (Clontech), pRetroX-Tight-GFP-puro (containing N-terminal GFP ...
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bioRxiv - Cell Biology 2021Quote: ... mCherry-STIM1-10A and WT STIM1 were constructed by inserting PCR fragments into XhoI-HpaI sites of pMSCV-puro vector (Clontech) and were packaged using the Phoenix-ECO cell line (ATCC CRL-3214) ...
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bioRxiv - Biochemistry 2020Quote: ... siRNA resistant WRN transgenes containing a C-terminal 3xFLAG tag (designated WRNr) were synthesized and inserted into the lentiviral pLVX-IRES-puro plasmid vector (ClonTech) at GenScript ...