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Citations for Takara Bio :
1 - 50 of 1523 citations for pLenti CMV TO Puro DEST 670 1 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: We constitutively expressed EWSR1-FLI1 constructs using a CMV driven pLVX-IRES-puro (Clontech) backbone ...
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bioRxiv - Developmental Biology 2020Quote: ... It was subsequently cloned into BamHI site in the pLenti CMV-IRES-GFP by In-Fusion cloning kit (Clontech, 639649) to generate lentiviral packaging plasmids(pLentiCMV-Y437H-IRES-GFP) ...
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bioRxiv - Immunology 2021Quote: The cDNA for different FcRs was cloned in fusion with EGFP in the pLVX-CMV-IRES-Puro plasmid (Clontech). The cDNA coding for FcγRI was amplified from the NM_000566 cDNA (Origene ...
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bioRxiv - Cancer Biology 2022Quote: ... The biosensor expression cassette was transduced into the stable tet-OFF MCF10A cell line under the regulation of a tet-inducible minimal CMV promoter (pRetro-X-Puro; Clontech) and selected using puromycin for a stably integrated population ...
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bioRxiv - Genomics 2024Quote: The protein coding sequence of CaGHV-1 ORF50 was PCR amplified and cloned into the pCDH-CMV-MCS-EF1-puro expression vector using In-Fusion cloning (TaKaRa). The cloning of the other gammaherpesvirus RTAs has been published in our previous study (54) ...
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bioRxiv - Molecular Biology 2023Quote: ... pLenti MYC:CTRL vector was modified from pLenti MYC:PKR by InFusion cloning (Takara Bio). PKR K296R and PKR T451A were obtained by InFusion mutagenesis (Takara Bio ...
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bioRxiv - Molecular Biology 2023Quote: ... in which the CMV promoter of pAAV-CMV vector (Takara Bio) was replaced by gfaABC1D ...
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bioRxiv - Microbiology 2020Quote: ... CSII-CMV-MCS-IRES2-Bsd (RIKEN BRC) and pLVSIN-CMV Pur (Takara Bio), respectively ...
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bioRxiv - Molecular Biology 2020Quote: ... pLVX-puro (Clontech), used for stable expression of Su9-(LbCas12a)-GFP ...
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bioRxiv - Developmental Biology 2020Quote: ... The CMV-MitoDsRed2 (pDsRed2-Mito) and CMV-MitoGFP (pAcGFP1-Mito) plasmids are from Clontech. Atoh7-MitoDsRed2 was designed using UGene software (Unipro) ...
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bioRxiv - Cell Biology 2024Quote: ... PGK-GFP was made by swapping the CMV promoter in CMV-EGFP-C1 (Clontech) vector with the hPGK promoter amplified from pLenti.PGK.blast-Renilla_Luciferase (Addgene 74444 ...
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bioRxiv - Immunology 2020Quote: ... 1.3 μg CMV 8.91 (Clontech), and 1.5 μg of target vector were transfected using TransIT-293T transfection reagent (Mirus Bio) ...
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bioRxiv - Cell Biology 2020Quote: ... pRetro-X-puro (Clontech) was modified by inserting a Gateway destination (-DEST ...
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bioRxiv - Cell Biology 2022Quote: ... pLVX-IRES-Puro plasmid (Clontech) was digested with AgeI and NotI (FastDigest ...
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bioRxiv - Cancer Biology 2020Quote: ... pLVX-Tetone-puro-empty (Clontech) construct was linearized with AgeI ...
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bioRxiv - Bioengineering 2024Quote: ... or pLVX-Puro (Clontech (TaKaRa)) ...
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bioRxiv - Bioengineering 2024Quote: ... or pLVX-Puro (Clontech (TaKaRa)) ...
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bioRxiv - Microbiology 2020Quote: ... The CMV-GFP construct was pEGFPC1 (Clontech). HIV-1 bearing a Ba-L envelope gene has been described (Rasaiyaah et al. ...
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bioRxiv - Bioengineering 2023Quote: ... CHAMP-1 or ErbB2TM cloned in the expression vector pRetroX-TIGHT-puro (Clontech) was introduced into cells expressing tTA by retroviral infection and selection with 1 µg/ml puromycin ...
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bioRxiv - Cell Biology 2022Quote: ... into a pRetroX-TetOne-Puro (Takara) backbone using the EcoRI and BamHI restriction sites ...
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bioRxiv - Molecular Biology 2022Quote: ... These coding gene sequences were all cloned into CMV-based FLAG-tagging and Clover-tagging vectors (CMV-FLAG-J1 and CMV-Clover-J1; derived from commercially available vectors pEGFP-C1 and pEGFP-N1; Clontech) for ectopic expression in human cells.
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bioRxiv - Neuroscience 2022Quote: ... Mover-IRES-palmGFP (promoter: CMV; backbone: Clontech N1); mGFP-Mover (promoter ...
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bioRxiv - Neuroscience 2020Quote: ... the CMV-EGFP plasmid (pEGFP; Clontech, CA, USA) was used as a positive control ...
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bioRxiv - Biophysics 2024Quote: ... with Lifeact-GFP plasmid (pLVX LifeAct-eGFP-P2A-puro, constructed from pLVX puro vector Clontech Catalog No. 632164), selected with 1 µg/ml puromycin and sorted by FACS ...
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bioRxiv - Immunology 2021Quote: ... and cloned into pLVX-IRES-Puro (Takara) via XhoI/NotI or EcoRI/NotI sites ...
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bioRxiv - Cancer Biology 2021Quote: ... bicistronic pLVX-EF1α-IRES-puro (Takara Bio) encoding the CDS of human PON1 single mRNA transcript was cloned as previously described ...
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bioRxiv - Molecular Biology 2022Quote: ... pLVX-Tet-One-Puro vector (Clontech, #631847), encoding for an engineered Tet activator and the corresponding inducible promoter ...
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bioRxiv - Cell Biology 2022Quote: ... followed by subcloning into pLVX-puro (Clontech). All constructs were sequenced before use ...
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bioRxiv - Immunology 2023Quote: ... and cloned into pLVX-IRES-Puro (Takara) via BamHI and BglII (V5-TurboID ...
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bioRxiv - Cell Biology 2022Quote: ... the CMV promoter from pEGFP-C1 (Takara Bio Inc) was removed and replaced with the SEPTIN2 promoter using AseI and AgeI ...
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bioRxiv - Cancer Biology 2024Quote: ... pHTN HaloTag® CMV-neo and pHTC HaloTag® CMV-neo were digested with restriction enzymes and subsequently ligated (Takara, cat n°6023). Primers and corresponding restriction digests can be found in Table S4 ...
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Ferroportin Depletes Iron Needed for Cell Cycle Progression in Head and Neck Squamous Cell CarcinomabioRxiv - Cancer Biology 2022Quote: The tet-inducible lentivirus vectors pLVX-TetOne-puro vector and pLVX-Tetone-puro-Lucif control vector were purchased from Takara Bio ...
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bioRxiv - Cancer Biology 2020Quote: ... PCR fragments were cloned into pLVX-puro (Clontech) lentivirus vector with Gibson Assembly Mixture (NEB ...
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bioRxiv - Bioengineering 2021Quote: ... We acquired pLVX-EF1α-IRES-puro from Takara Bio Inc ...
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bioRxiv - Cell Biology 2022Quote: ... the MSCV-puro vector (Takara Bio USA, #634401) were used ...
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Receptor-like role for PQLC2 amino acid transporter in the lysosomal sensing of cationic amino acidsbioRxiv - Cell Biology 2020Quote: ... stably expressing PQLC2-FLAG (pLVX-puro vector, Clontech). Proteins bound to glutathione resin were then separated by SDS-PAGE and immunoblotted with anti-FLAG antibodies while the GST-tagged proteins were visualized by Ponceau S staining (APACOR) ...
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bioRxiv - Cancer Biology 2024Quote: ... and pLVX-E1Fα-IRES-mCherry-Puro (Takara Bio) to perform the experiments associated with its ectopic expression ...
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bioRxiv - Cell Biology 2021Quote: ... pEGFP and mCherry vectors were from Clontech (with CMV promoter) with either C1 or N1 cloning sites to obtain the tag either at the N-terminus or C-terminus of Arc ...
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bioRxiv - Molecular Biology 2020Quote: ... 2.5 μg of PrimPol variant expressing pcDNA3.1/nV5-DEST vectors11 or control plasmid pEGFP-C2 (Clontech) were transfected for the last 24 h using TransIT-2020 (Mirus Bio) ...
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bioRxiv - Cell Biology 2022Quote: ... PRR14-GFP 1-212 PCR products were sub-cloned into pLVX-TetOne-Puro (Takara Bio, cat# 631847) vector using T7 ligase (New England Biolabs ...
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bioRxiv - Cancer Biology 2021Quote: ... with PCR using the primers listed in Table 1 and cloned into pmCherry-C1 (CMV promoter; Takara Bio Inc.). pEGFP-C3 K19 WT ...
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bioRxiv - Cell Biology 2021Quote: ... followed by cloning into a pLVX-puro vector (Clontech) using the XhoI and BamHI restriction sites (primers in Suppl ...
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bioRxiv - Developmental Biology 2022Quote: ... the IRES sequence from pLVX-IRES-Puro (Clontech; 632186), and sfGFP (superfolder GFP ...
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bioRxiv - Microbiology 2022Quote: ... and pLVX-EF1α-IRES-Puro was purchased from Clontech Laboratories (plasmid #631988).
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bioRxiv - Cell Biology 2022Quote: ... into the EcoRI site of pLVX-puro vector (Clontech) or into the BamHI/NotI site of pRetroX-Tight-puro vector (Clontech) ...
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bioRxiv - Developmental Biology 2020Quote: ... was cloned into pLVX Tet-One Puro plasmid (Clontech), packaged in 293T cells (from ATCC ...
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bioRxiv - Cancer Biology 2021Quote: ... Using the combination with pLVX-Tight-puro vector (Clontech), doxycycline-mediated inducible expression clone of cancer cells were generated by screening with 2 μg/ml puromycin and 600 μg/ml Zeocin (ThermoFisher Scientific).
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bioRxiv - Cancer Biology 2022Quote: ... and ligated into the pLVX-Puro vector (Takara #632164) between the PspOMI and BamHI restriction sites by using T4 DNA ligase (New England Biolabs #M0202) ...
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bioRxiv - Molecular Biology 2024Quote: ... Lentivirus transduction vectors were derived from pLVX-puro (Clontech). For β-galactosidase transduction ...
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bioRxiv - Biochemistry 2024Quote: ... were transfected with a pLVX-TetOne-Puro vector (Takara) containing a gene encoding human MDA5 (UniProt ...