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Citations for Takara Bio :
51 - 100 of 1523 citations for pLenti CMV TO Puro DEST 670 1 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... encoding EGFP under the control of CMV promoter was from Clontech. pReceiver-EGFP (M02Rx ...
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bioRxiv - Molecular Biology 2024Quote: ... pLenti V HELLS WT or Mutant plasmids were constructed using In-Fusion cloning (Takara Bio) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... a lentiviral constitutive expression vector pLVX-EF1alpha-IRES-Puro (Takara) that carries the ORF insert (provided by Dr ...
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bioRxiv - Cell Biology 2020Quote: The retroviral plasmid pRetroX-TetOne puro was acquired from Clontech and amplified using NEB Stable competent E ...
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bioRxiv - Cell Biology 2021Quote: ... or into the EcoRI site of pLVX-puro vector (Clontech). pLVX-puro-DELE1-HA and pLVX-puro-Su9-mCherry was created by PCR amplification and sub-cloning into the EcoRI site of pLVX-puro vector ...
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bioRxiv - Cell Biology 2022Quote: ... and was cloned into the pLVX-IRES-puro vector (Clontech). Paxillin-pEGFP was a gift from Rick Horwitz (Addgene plasmid # 15233 ...
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bioRxiv - Cell Biology 2022Quote: ... and was cloned into the pLVX-IRES-puro vector (Clontech). The GFP-AktPH construct was obtained from the laboratory of Jason Haugh (North Carolina State University ...
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bioRxiv - Cell Biology 2022Quote: ... and was cloned into the pLVX-IRES-puro vector (Clontech). The BioID2 construct was obtained from Addgene (Addgene plasmid # 74223 ...
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bioRxiv - Cell Biology 2024Quote: ... pEGFP-C1 and pLVX-IRES-Puro were purchased from ClonTech. Guide RNA sequences were cloned into pSpCas9(BB)-2A-GFP (pX458) ...
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bioRxiv - Cancer Biology 2024Quote: 1,000,000 Rh41 cells were transfected with 2 μg of CMV-GFP (Clontech) and 200nM of siRNA using the Invitrogen Neon Transfection System (Voltage 1050V ...
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bioRxiv - Cancer Biology 2020Quote: ... Genes were transferred into the lentiviral vector pLVX-Tight-Puro (Clontech) modified to contain a C-terminal V5 tag (pLVX-V5 ...
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bioRxiv - Microbiology 2022Quote: ... the cDNA was transferred to pLVX-EF1alpha-IRES-Puro (Takara/Clontech) by classic restriction digest and ligation ...
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bioRxiv - Microbiology 2022Quote: ... the cDNA was transferred to pLVX-EF1alpha-IRES-Puro (Takara/Clontech) by classic restriction digest and ligation ...
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bioRxiv - Cancer Biology 2022Quote: ... amplified from the linear puro marker (Cat. No. 631626, Takara-Bio), was inserted using the In-fusion HD cloning kit (Cat ...
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bioRxiv - Cancer Biology 2023Quote: ... the fragments were subcloned into the pLVX-puro vector (Takara, #632164) with Ha- or Flag-tag ...
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bioRxiv - Molecular Biology 2023Quote: ... C-FLAG-D20 was subcloned into the pLVX-TetOne-Puro (Clontech) and in-house attb vectors ...
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bioRxiv - Molecular Biology 2023Quote: ... APEX2 and p110 were cloned into pLVX-IRES-puro (Takara, 632183) via a series of restriction enzyme digests and ligations ...
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bioRxiv - Cell Biology 2022Quote: ... and the construct was cloned into pLVX-IRES-puro vector (Clontech). SEPT6(ΔAH)-GFP was generated by removing aa 355-372 of SEPT6 from SEPT6-GFP-pLVX-IRES-puro via PCR ...
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bioRxiv - Cancer Biology 2024Quote: ... into the EcoRI-SmaI sites of the pLVX-Puro vector (Clontech).
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bioRxiv - Cancer Biology 2024Quote: ... gene blocks were assembled with a digested pLVX-Tight-Puro (ClonTech) or pLENTI-CMV-GFP-Puro (Addgene ...
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bioRxiv - Immunology 2024Quote: ... and HLA-B*07:02 were synthesized by GenScript and cloned into a pLVX-EF1α-IRES-Puro lentiviral vector (Clontech). Lentivirus was produced by transfecting HEK 293T cells (ATCC CRL-3216 ...
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bioRxiv - Neuroscience 2023Quote: ... Cousin lab was dropped into an pEGFP-N1 CMV mammalian expression vector (Clontech) by In-fusion cloning ...
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bioRxiv - Neuroscience 2024Quote: The mammalian expression vector pBApo-CMV-Pur (cat. #3421) was purchased from Takara. We used Genescript to insert the 144 amino acid sequence for WT synuclein into the backbone and create 6 mutated strains ...
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bioRxiv - Microbiology 2022Quote: ... and A549-MxB(1-715)-MxB(26-715) cells expressing untagged MxB(1-715) and MxB(26-715) using the pLKOD-Ires-Puro vector (Clontech Takara Bio ...
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KIF24 controls the clustering of supernumerary centrosomes in pancreatic ductal adenocarcinoma cellsbioRxiv - Cell Biology 2022Quote: ... human KIF24 fragments encoding residue 1-4383 was excised from pEGFP-C1-KIF24 (Kobayashi et al., 2011) and sub-cloned into pLVX-IRES-Puro (Clontech). KIF24/TS621-622AA (TS ...
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bioRxiv - Cell Biology 2022Quote: ... Wild-type iRhom2 and iRhom2-1-374 were also cloned by PCR into pM6P.Blasticidin (for retroviral infection) and into pLVX-TetOne-Puro vector (Clontech, #631849) (for lentiviral infection) ...
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bioRxiv - Microbiology 2024Quote: ... HEK293T cells were co-transfected with the resulting vector pLVX-Puro-GFP-1-10 and the packaging and envelope plasmids using Lenti-X Packaging Single Shots (Takara). Supernatants containing lentiviral particles were collected at 48 and 72 hours post-transfection ...
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bioRxiv - Microbiology 2023Quote: ... and cynomolgus monkey were cloned into a pLVSIN-CMV Hyg vector (TaKaRa, Cat# 6182) predigested with NotI-HF (NEB ...
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bioRxiv - Neuroscience 2024Quote: ... Amplicons were inserted into a pAAV-CMV vector using In-Fusion cloning (Takara Bio). Plasmids were verified with Sanger sequencing (Genewiz ...
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bioRxiv - Cell Biology 2020Quote: ... the 1.3 kb Puro cassette was amplified by PCR using pPur (Clontech) as template and inserted into the Asp718 and BamHI sites of pEGFP-C1 to yield pEGFP-C1 Puro ...
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bioRxiv - Cancer Biology 2022Quote: The retroviral plasmid pRetroX-TetOne puro (634307, Clontech, Mountain View, CA, USA) was amplified using NEB Stable competent Escherichia coli (high efficiency ...
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bioRxiv - Cell Biology 2022Quote: ... or into the BamHI/NotI site of pRetroX-Tight-puro vector (Clontech). The original vector of C-terminally HA-tagged Su9-TEV protease was gifted from Dr ...
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bioRxiv - Cancer Biology 2022Quote: ... then cloned into the doxycycline inducible pLVX-pTetOne-puro vector (Takara Bio) using In-Fusion HD (Takara Bio ...
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bioRxiv - Cell Biology 2020Quote: ... The sequence was ligated into the pLVX Puro lentiviral plasmid (Clontech, CA) using the same restriction sites and sequenced to confirm correct orientation ...
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bioRxiv - Cell Biology 2020Quote: ... We first exchanged the puromycin resistance gene in pLVX-TetOne-puro (Clontech) with neomycin to generate pLVX-TetOne-neo ...
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bioRxiv - Cancer Biology 2021Quote: ... Obtained cDNAs were sub-cloned into pLVX-Puro lentiviral expression vector (Clontech) and transfected to HEK293T cells together with packaging plasmids psPAX2 and pMD2G using Lipofectamine 2000 (Invitrogen) ...
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bioRxiv - Cell Biology 2024Quote: ... was subcloned into the lentiviral expression vector pLVX-M-puro (Takara 632164). For CRISPR-KO experiments ...
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bioRxiv - Biochemistry 2024Quote: ... pUB83 is a lentiviral expression vector based on the pLVX-PURO (Clontech) plasmid containing a CMV promoter ...
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bioRxiv - Microbiology 2020Quote: ... we PCR amplified HXB2 env from the pIIIenv3-1 plasmid (NIH AIDS Reagent Program cat. #289) and cloned it into the pLVX-EF1α-IRES-Puro lentiviral vector (Clontech cat. #631988). J-Lat clone 11.1 cells were transduced with lentiviral particles produced from this plasmid and stable cell lines were generated using puromycin selection.
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bioRxiv - Biophysics 2021Quote: ... Protein constructs were expressed under control of a CMV promoter using pEGFP-C1/N1 (Clontech) (enhanced GFP ...
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bioRxiv - Molecular Biology 2024Quote: ... rat AT1 and RlucII were subcloned into the pLVSIN-CMV Neo vector (Takara Bio, Japan). Next ...
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bioRxiv - Biochemistry 2021Quote: ... viral particles were first generated in HEK-293FT cells using PVLX-puro (Clontech) alongside psPAX2 and pMD2.G obtained from Addgene ...
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bioRxiv - Cell Biology 2022Quote: Mouse Itgb3 was cloned into the pLVX-EF1α-IRES-Puro vector (Takara Bio) per a previously described cloning strategy (Dave et al. ...
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bioRxiv - Cancer Biology 2022Quote: Genes in the Hippo pathway were subcloned into pLVX-puro vector (Takara, #632164) with HA- ...
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bioRxiv - Cell Biology 2023Quote: ... 3xFLAG-SMARCAL1 cDNAs were cloned into the lentiviral vector pLVX-TetOne-Puro (Clontech) by Gibson Assembly ...
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bioRxiv - Immunology 2023Quote: ... All constructs were cloned in the expression vector pLVX-Tight-Puro (Clontech Laboratories), between 5’ NotI and 3’ EcoRI restriction sites ...
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bioRxiv - Cancer Biology 2023Quote: ... All constructs were cloned into pLVX-IRES-Puro (modified from pLVX vector (Clontech) by removal of the ZsGreen and replacement with a multiple cloning site and IRES-Puro ...
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bioRxiv - Cancer Biology 2024Quote: ... This fragment was cloned into the pLVX-EF1α-IRES-Puro vector (Clontech, #631988) by first treating with the EcoR1-HF (NEB ...
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bioRxiv - Cell Biology 2024Quote: ... and Cytb5 were inserted into the lentiviral plasmid pLVX-TetOne-Puro (631849; Clontech) for doxycycline-dependent expression ...
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bioRxiv - Cell Biology 2021Quote: ... The DCP1 and RNaseL amplicons were inserted into Xho1/Xba1 cleaved pLenti-EF1-Blast vector using In-Fusion (Clontech). All insert sequences were confirmed by sequencing.