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Citations for Takara Bio :
151 - 200 of 1155 citations for Human PDGF alpha receptor PDGFRA qPCR Primer Pair since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... A 5S rRNA reverse transcript generated using random primers (TaKaRa) was used as a reference ...
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bioRxiv - Microbiology 2023Quote: ... We used Primer/Probe N2 (2019-nCoV) (TaKaRa Bio Inc.). The reaction conditions for RT-PCR were 42 °C for 5 min and 95 °C for 10 s ...
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Bipartite viral RNA genome heterodimerization influences genome packaging and virion thermostabilitybioRxiv - Molecular Biology 2022Quote: The primers used to generate the In-Fusion cloning (TaKaRa) fragments were listed in Table S1 ...
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bioRxiv - Immunology 2022Quote: ... oligo-dT primer (2.5 pmol) and RNAse inhibitor (1U – Clontech). cDNA generation and amplification was performed following the SmartSeq2 approach 26 ...
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bioRxiv - Genetics 2024Quote: ... and indexing primers (DNA HT Dual Index kit, Takara Bio) with all procedures performed as per the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... using primers listed in Table S1 (designed using the Takara in-Fusion Cloning Design Primer tool (https://takara.teselagen.com/#/DesignPage)) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... for S100a9 with a primer set (MA058882) obtained from TaKaRa Bio Inc ...
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bioRxiv - Cell Biology 2020Quote: Human CDC20 was amplified from human testis cDNA (Marathon cDNA; Takara Bio Inc.) using Pfu polymerase (Agilent Technologies) ...
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bioRxiv - Microbiology 2024Quote: High-throughput qPCR was performed by an outside firm (Resistomap, Helsinki, Finland) using the qPCR SmartChip Real-Time PCR cycler (Takara; Kusatsu, Japonia). The qPCR cycling conditions and initial data processing were carried out as previously described by Wang et al ...
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bioRxiv - Genomics 2021Quote: ... Takara Premix Ex Taq (Probe qPCR) (TaKaRa, Kyoto, Japan), Designs of PCR probes ...
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bioRxiv - Bioengineering 2023Quote: ... quantified via qPCR (AAVpro Titration Kit version 2; Clontech), and stored at 4°C until use.
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bioRxiv - Molecular Biology 2022Quote: ... The 2× SYBR Green qPCR Mix Kit (TaKaRa, Japan) and the cDNA concentration and primers described above were used ...
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bioRxiv - Microbiology 2024Quote: ... A Premix Ex Taq™ (Probe qPCR) (TaKaRa, Japan) was used to perform qPCR following manufacturer’s instructions and each reaction consisted of 1 μl DNA template ...
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bioRxiv - Microbiology 2023Quote: ... and the Premix Ex Taq (probe qPCR) kit (Takara). All PCRs were performed in a StepOne plus instrument (Applied Biosystems) ...
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bioRxiv - Developmental Biology 2023Quote: ... qPCR was performed using SYBR green PCR mix (TAKARA) in Roche Light Cycler.
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bioRxiv - Physiology 2024Quote: ... for qPCR using TB Green Premix Ex Taq (Takara) on a ViiA 7 Real-Time PCR system (Applied Biosystems) ...
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bioRxiv - Cell Biology 2022Quote: ... The extracted probes were radioactively labeled with 2.5 μl [alpha-32P] dCTP (Amersham) according to the instructions of the kit (LaddermanTM Labeling Kit, Takara). The probe was mixed with 100 μl TE buffer (10mM ...
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bioRxiv - Microbiology 2020Quote: ... Quantitative PCR (qPCR) analysis of intracellular HIV-1 DNA levels was conducted using Premix Ex Taq (Probe qPCR) Rox plus (Takara Bio, Kusatsu, Japan). The oligonucleotides HIV-1 LTR and β2-microglobulin were used for HIV-1 DNA quantification and cell number determination ...
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bioRxiv - Immunology 2020Quote: ... The entirety of each of the annotated intracellular domains was ordered as a separate gene fragment (Integrated DNA Technologies) and each complete receptor was cloned into pHR lentiviral expression vector (Clontech). Each region of the protein was amplified using PCR and fragments were combined using Gibson assembly.
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bioRxiv - Cell Biology 2023Quote: HeLa cells and hippocampal neurons were transfected with plasmids encoding FM4 recombinant receptors for 15 h and then treated with 2 μM DD-Solubilizer (TakaraBio/Clontech) to induce the release of the receptors from the ER into the secretory trafficking ...
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bioRxiv - Immunology 2023Quote: T cell receptors were generated by custom synthesis (Twist Bioscience) in a 3rd generation pLVX-EF1α-IRES-Puro lentiviral vector (Takara/Clontech). Following the TransIT-Virusgen Transfection (Mirus ...
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bioRxiv - Synthetic Biology 2024Quote: ... cells were transiently transfected with plasmids containing receptors of interest under eukaryotic expression promoters (ASGR1 was clone OHU03658D from GenScript, negative control pAMCyan1 from Takara), then split into 96-well plates (20,000 cells per well ...
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bioRxiv - Plant Biology 2020Quote: ... while specific AD and BD recombinant plasmid pairs were used to co-transform yeast strain AH109 cells according to the Yeast Protocols Handbook (Takara, Dalian, China).
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bioRxiv - Plant Biology 2022Quote: ... the entire ELF3 genomic sequence present in three HIF pairs was amplified using Ex Taq DNA Polymerase (Takara Bio, Kusatu, Shiga, Japan) and 1186 bp of promoter sequence upstream of the ELF3 start codon in HIF pair 10 was amplified using ALLin™ RPH Polymerase (highQu GmbH ...
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bioRxiv - Immunology 2021Quote: ... All custom oligos and the commercial universal primer short (Takara USA) were used at a final concentration of 0.2 μM ...
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bioRxiv - Cancer Biology 2020Quote: ... Day 17 embrionic RNA was used to validate primers (Clontech/Takara). cDNA was synthesised with Superscript III (Invitrogen) ...
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bioRxiv - Cancer Biology 2020Quote: ... Day 17 embrionic RNA was used to validate primers (Clontech/Takara). cDNA was synthesised with Superscript III (Invitrogen) ...
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bioRxiv - Microbiology 2020Quote: ... or Oligo dT-3 sites Adaptor Primer for 3’ RACE (Takara). To analyze the complete BToV genome ...
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bioRxiv - Cancer Biology 2023Quote: ... and oligo dT and random hexamer primers were used together (Takara). A Q6 qPCR System was used with TB Green Premix Ex Taq (Takara) ...
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bioRxiv - Cell Biology 2021Quote: The cDNA encoding human BORCS6 was cloned from human lung total RNA (Takara Bio, Japan) and inserted into pCR4-TOPO (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: Human MPS1 and NSL1 were amplified from Human testis cDNA (Marathon cDNA; Takara Bio Inc.) using Pfu polymerase (Promega) ...
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bioRxiv - Microbiology 2022Quote: ... RT-qPCR was performed using SYBR Premix Ex Taq (Takara). Roche LightCycler 480 thermal cycler was used to assess the melting curve and quantification of the PCR products ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... qPCR analysis was performed using SYBR Premix Ex Taq (Takara) on a 7900 Fast Real-Time PCR system (Applied Biosystems ...
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bioRxiv - Bioengineering 2024Quote: TB Green Advantage qPCR premix (Takara Bio, Cat. No. 639676) was used for the amplification of osteogenic and chondrogenic markers ...
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bioRxiv - Developmental Biology 2024Quote: ... qPCR was performed using TB Green Premix Ex Taq (Takara). The ΔCt method using Gapdh as a reference gene was used to analyse relative expression and the 2−ΔΔCt (Livak ...
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bioRxiv - Microbiology 2024Quote: ... The cDNA was used for performing qPCR with Taqman (Takara). During setup of the qPCR reaction ...
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bioRxiv - Neuroscience 2024Quote: ... RT-qPCR was conducted using TP-850 (Takara Bio, Japan) and TB green Taq (Takara Bio ...
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bioRxiv - Immunology 2023Quote: T cell receptors were generated by custom synthesis (Twist Bioscience) in a 3rd generation pLVX-EF1α-IRES-Puro lentiviral vector (Takara/Clontech). Following the TransIT-Virusgen Transfection (Mirus ...
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bioRxiv - Microbiology 2020Quote: ... The devH coding region amplified by PCR using the primer pair NHisdevH-F1 and NHisdevH-R1 was cloned between the NdeI and SalI sites of the expression vector pColdProS2 (Takara Bio, Shiga, Japan) to construct pCProS2DevH ...
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bioRxiv - Cell Biology 2021Quote: ... The nucleotide sequence encoding for human CyPD was obtained from a Human cDNA placenta library (Clontech) by PCR ...
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bioRxiv - Developmental Biology 2024Quote: ... Full-length cDNA fragment of human MAK was amplified by PCR using human retinal cDNA (Clontech) as a template and subcloned into the pCAGGSII-2xHA vector (62) ...
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bioRxiv - Cancer Biology 2020Quote: ... using the Nextera Primer P5 (ICELL8® 3’ DE Kit, Takara Bio), Nextera XT DNA Library Preparation Kit (Illumina ...
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bioRxiv - Microbiology 2022Quote: ... a set of primer/probe E484A (SARS-CoV-2) (Takara, Cat# RC322A) was used ...
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bioRxiv - Cancer Biology 2020Quote: ... molecular enzymes and primers used in this study were purchased from Takara Bio ...
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bioRxiv - Developmental Biology 2023Quote: ... using In-Fusion cloning (Takara Bio, primers are listed in Table S3) and then inserted into a pDestTol2ACryGFP backbone (Berger and Currie ...
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bioRxiv - Molecular Biology 2023Quote: ... primer 0.2 μl (200 pmol/μl) and ExTaq HS 0.1 μl (TAKARA), and PCR analysis was performed under the following conditions ...
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bioRxiv - Microbiology 2023Quote: ... All the primers were designed using an online tool provided by TaKaRa (https://www.takarabio.com/learning-centers/cloning/primer-design-and-other-tools ...
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bioRxiv - Physiology 2022Quote: ... first-strand cDNA was synthesized with 5’ RACE CDE Primer A (Clontech) (5’-RACE-Ready cDNA samples) ...
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bioRxiv - Genomics 2024Quote: ... Dual-indexed primers (5μM) 2.0μL in Unique dual index kit (Takara bio) was used instead of 0.5 μL Index Primer in the default Zymo kit ...
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bioRxiv - Biochemistry 2024Quote: ... Primers were designed using a PrimeSTAR Mutagenesis Basal Kit (Takara Bio, Inc.) according to the manufacturer’s protocol ...