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Citations for Takara Bio :
101 - 150 of 912 citations for pIEXBac c EGFP 3 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... encoding EGFP under the control of CMV promoter was from Clontech. pReceiver-EGFP (M02Rx ...
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bioRxiv - Cell Biology 2020Quote: ... was subcloned by replacing the EGFP sequence of pEGFP-N1 (Clontech, Takara) [24] ...
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bioRxiv - Cell Biology 2020Quote: ... was subcloned by replacing the EGFP sequence of pEGFP-N1 (Clontech, Takara) [24] ...
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bioRxiv - Neuroscience 2020Quote: ... Flow cytometry calibration beads for AcGFP1/eGFP and mCherry (Takara, 632594, 632595) were used as fluorescence intensity controls ...
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bioRxiv - Biophysics 2021Quote: ... and subcloned into the AgeI- and SalI-digested EGFP-C2 vector (Clontech). The primer sequence of SmBiT was designed from pBiT2.1-N [TK/SmBiT] Vector (Promega).
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bioRxiv - Cell Biology 2022Quote: ... FcγRIIA-EGFP or Lifeact-mCherry was cloned into pAdenoX-Tet3G (Takara Bio), and adenovirus was prepared according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... Zebrafish PAC coding sequence (NP_001278691) was subcloned into pIRES2-EGFP vector (Clontech) using NheI and EcoRI restriction enzyme sites ...
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bioRxiv - Cell Biology 2022Quote: ... then imaged after overnight expression and ∼3 hour incubation with 500 nM A/C heterodimerizer linker drug (Takara, 635055) or 100% ethanol ...
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bioRxiv - Immunology 2022Quote: ... Sequences encoding DCFHP (residues 1-1146 of HexaPro)2 and SΔC-Fer (residues 1-1143 as previously described)3 were cloned into the pADD2 vector backbone using HiFi PCR (Takara) followed by In-Fusion (Takara ...
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bioRxiv - Neuroscience 2021Quote: ... the fluorescent proteins were amplified from pAM FLEX eGFP and pmCherry-C1 (Clontech) respectively ...
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bioRxiv - Molecular Biology 2022Quote: ... was based on a pIRES2-EGFP vector (Clontech, Mountain View, CA; #6029-1). cDNA inserts from coding domain sequences (CDS ...
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bioRxiv - Plant Biology 2022Quote: ... and EGFP was inserted using In-Fusion® cloning (Takara Bio Cat. #121416). For p35S::DAAO-GFP ...
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bioRxiv - Cell Biology 2020Quote: ... S5C-E was generated by fusing human Rac1 cDNA into EGFP-C1 (Clontech) and kindly provided by Dr ...
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bioRxiv - Cell Biology 2021Quote: ... we generate the plasmid pUAS-FMR1-ADARcd-V5-T2A-EGFP by Infusion (TaKaRa) cloning of the three PCR fragments ...
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bioRxiv - Genetics 2023Quote: ... Slices were then incubated with anti-EGFP antibody (Takara Clontech 6323380; 1: 5.000) and anti ISL1 (AbCam cat no ab86472 ...
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bioRxiv - Genetics 2023Quote: ... Slices were then incubated with anti-EGFP antibody (Takara Clontech 6323380; 1: 5.000) and anti ISL1 (AbCam cat no ab86472 ...
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bioRxiv - Cell Biology 2023Quote: For mammalian expression of eGFP labelled proteins we used the pEGFP-C1 (Clontech) vector ...
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bioRxiv - Physiology 2024Quote: ... (Cx43: 1:3000 custom-produced rabbit polyclonal; eGFP: 1:1000 mouse monoclonal (Clontech Laboratories Inc ...
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bioRxiv - Molecular Biology 2022Quote: EGFP and TDP-43EGFP cDNAS were amplified from pEGFP-C1 (Clontech, 6084-1) and pEGFP-C1-TDP-43 (kind gift from Dr ...
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bioRxiv - Neuroscience 2024Quote: ... the fluorescent proteins were amplified from pAM FLEX eGFP and pmCherry-C1 (Clontech) respectively ...
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bioRxiv - Microbiology 2024Quote: ... The pReplacer-eGFP-FAM111B was generated by inserting FAM111B into peGFP-C1 (Clontech) with EcoRI and BamHI ...
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bioRxiv - Genetics 2024Quote: ... plus MTP38 (5’ – CTG GAG TTC AGA CGT GTG CTC TTC CGA TCT CCA GAA GTC TTA ACT GAT GAT CGG C – 3’) with PrimeSTAR Max DNA Polymerase (Takara Bio) to amplify a portion of Rad51 with universal Illumina adaptor 5’ overhangs ...
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bioRxiv - Neuroscience 2020Quote: ... which was generating by replacing a V5 tag with an eGFP or DsRed (Clontech) tag from pcDNA3.1-nV5-DEST vector (Invitrogen) ...
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bioRxiv - Biochemistry 2022Quote: ... was added at the N-termini of EGFP and mCherry (Clontech, Mountain View, CA). For intramolecular FRET experiments ...
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bioRxiv - Plant Biology 2022Quote: ... EGFP was inserted using HindIII and In-Fusion® cloning (Takara Bio Cat. #121416). For pAGO9::EGFP-AGO9 ...
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bioRxiv - Physiology 2021Quote: Plasmid DNA encoding enhanced green fluorescent (EGFP)-actin (Takara Bio USA, Mountain View, CA) facilitated exogenous gene expression in rodent kidneys ...
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bioRxiv - Immunology 2023Quote: ... As a negative control for pull-downs assays the eGFP plasmid (Clontech Laboratories, USA) was transfected.
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bioRxiv - Biophysics 2022Quote: The coding sequence of human PAC (NP_060722) previously subcloned into pIRES2-EGFP vector (Clontech) using XhoI and EcoRI restriction enzyme sites9 was used for whole-cell patch clamp recording ...
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bioRxiv - Cell Biology 2024Quote: ... PGK-GFP was made by swapping the CMV promoter in CMV-EGFP-C1 (Clontech) vector with the hPGK promoter amplified from pLenti.PGK.blast-Renilla_Luciferase (Addgene 74444 ...
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bioRxiv - Microbiology 2021Quote: ... at 30°C for 3-5 days and assayed for growth on the SD/-Trp/-Leu/-His/-Ade/X-α-gal plates (TaKaRa Bio). Each experiment was repeated at least three times.
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bioRxiv - Cancer Biology 2021Quote: ... plasmid or the enhanced green fluorescent protein (EGFP) plasmid (pEGFP-C1, Clontech/Takara Bio Europe). Transfections were achieved using Lipofectamine 2000 (Invitrogen ...
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bioRxiv - Cancer Biology 2021Quote: ... plasmid or the enhanced green fluorescent protein (EGFP) plasmid (pEGFP-C1, Clontech/Takara Bio Europe). Transfections were achieved using Lipofectamine 2000 (Invitrogen ...
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bioRxiv - Biochemistry 2022Quote: ... DNA-lipid complexes were prepared by incubating 100 ng of eGFP-N1 plasmid (Takara Biosciences) with Lipofectamine 2000 for 20 min ...
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bioRxiv - Immunology 2021Quote: ... and cDNA encoding human full-length MuSK was cloned into pIRES2-EGFP plasmid vector (Clontech). AChR and MuSK vectors were kindly provided by Drs ...
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bioRxiv - Microbiology 2023Quote: ... USA) and subcloned into the NheI and XhoI restriction sites of plasmid pIRES2-EGFP (Clontech, Takara Bio USA ...
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bioRxiv - Molecular Biology 2023Quote: ... Signals for eGFP and FLAG were detected by using Mouse Anti-GFP JL-8 (Clontech), Mouse Anti-Flag (Sigma Aldrich ...
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bioRxiv - Plant Biology 2022Quote: ... eGFP was immuno detected with anti-GFP antibody (1:4000 dilution) (JL-8, #632380, Takara) and anti- mouse-HRP (1:15000 dilution ...
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bioRxiv - Neuroscience 2024Quote: ... and the corresponding DNA amplicons were cloned into the mammalian expression vector pIRES2-EGFP (Clontech) using restriction enzymes listed in Table S5 ...
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bioRxiv - Biophysics 2024Quote: ... human CALM1 cDNA within the pIRES2-EGFP vector was utilized for WT CaM (Clontech Laboratories). From this WT CaM-pIRES-EGFP construct ...
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bioRxiv - Microbiology 2023Quote: ... or the BamHI/MluI site of pWPI-ACE2-zeo (for ACE2 expression plasmids)43 with 3×FLAG-tag at the C-terminus using In-Fusion® HD Cloning Kit (Takara, Cat# Z9650N). Nucleotide sequences were determined by DNA sequencing services (Eurofins) ...
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bioRxiv - Neuroscience 2020Quote: ... 341F (5’-CCTACGGGNGGCWGCAG-3’) and 805R (5’-GACTACHVGGGTATCTAATCC-3’) or (2) 341F (5’-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCCTACGGGNGGCWGCAG-3’) and 806R (5’-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGGACTACHVGGGTWTCTAAT-3’) (TaKaRa Bio, Shiga, Japan). The second PCR was performed to add the index sequences for Illumina sequencing with barcode sequences ...
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bioRxiv - Cell Biology 2020Quote: ... EGFP was removed from pEGFP-C1 PLCdelta-PH and replaced with mCherry from pmCherry-C1 (TaKaRa). To create pmCherry-C1 RBDrhotekin ...
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bioRxiv - Cell Biology 2021Quote: ... The construct for cytosolic expression of RpHLuorin2 was generated by replacing EGFP in pEGFP-N1 (Clontech) with synthesized RpHLuorin2 using restriction sites AgeI and BsrGI ...
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bioRxiv - Cell Biology 2021Quote: ... and then EGFP-C20 fragment was inserted and ligated by In-Fusion cloning kit (638910; Clontech).
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bioRxiv - Neuroscience 2021Quote: ... intron 20 and exon 21 sequence was synthesised and cloned into a pIRES-EGFP vector (Clontech) by BioCat ...
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bioRxiv - Molecular Biology 2023Quote: ... EGFP-SEC61B was generated by cloning the corresponding cDNA into the pEGFP.C1 vector (Clontech, 6084-1). The plasmid pRK5-HA-UB was described previously [56] ...
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bioRxiv - Developmental Biology 2024Quote: ... pAAVS1-CRYS-ARL13B-bPAC-EGFP plasmid was constructed using In-Fusion HD Cloning Kit (Takara, 639650). The backbone sequence was cloned from pAAVS1-P-CAG-mCh plasmid (Addgene 80492100 ...
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bioRxiv - Cell Biology 2024Quote: ... The amplified PCR products were digested with restriction enzymes and inserted into EGFP-C1 vector (Clontech).
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and glyceraldehyde 3-phosphate dehydrogenase (5′-GCACCGTCAAGGCTGAGAAC-3′ and 5′-TGGTGAAGACGCCAGTGGA-3′; Takara Bio Inc., Shiga, Japan) were used for RT-PCR.
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bioRxiv - Evolutionary Biology 2020Quote: ... 3 (Takara, Shiga), which is capable of resistance/nonspecific amplification and smear suppression against such PCR-inhibiting substances ...