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Citations for Takara Bio :
1 - 50 of 912 citations for pIEXBac c EGFP 3 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: eGFP: EGFP (pAcGFP1-N1, Clontech)
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bioRxiv - Cell Biology 2020Quote: ... After 24 h cells were transfected with an eGFP vector (3 μg; Clontech) using Fugene-6 HD reagent according to the manufacturer's protocol ...
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bioRxiv - Bioengineering 2024Quote: ... mScarlet3 from pDx_mScarlet3 (a gift from Dorus Gadella [Addgene plasmid #189754]),135 IRES-EGFP from pIRES2-EGFP (Clontech), and IRES-E3 and IRES-PuroR (puromycin resistance ...
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bioRxiv - Cell Biology 2020Quote: ... in which EGFP cDNA of pIRES2-EGFP (Clontech) was replaced by Sirius fluoresent protein cDNA (Tomosugi et al. ...
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bioRxiv - Neuroscience 2022Quote: ... eGFP (Clontech), pCAG_GPHN.FingR-eGFP-CCR5TC [23] (gift from Don Arnold ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... with the PCR product of the W56-EGFP-Linker-Synapsin1a region from pAAV-hSyn1-W56-EGFP-Linker-Synapsin1a (FWD and REV primers: 5’-GGCGCGCCCTAGAATTTCAGTCGGAGAAGAGGCTGGC-3’ and 5’-ATGCTAGGCCACCATGATGGTGGACGGCAAGCCC-3’, respectively) using In-Fusion cloning (TaKaRa Bio). pAAV-hSyn1-DIO-Scr-EGFP-Linker-Synapsin1a was generated by replacing the TurboID region of pAAV-hSyn1-DIO-TurboID (EcoRI/NcoI sites ...
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bioRxiv - Developmental Biology 2022Quote: c-Kit-EGFP fusion protein: chicken c-Kit cDNA was inserted into multiple cloning site of pEGFP-N1 Vector (Clontech, #6085-1) including EGFP sequences using a primer set ...
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bioRxiv - Microbiology 2021Quote: ... EGFP (from Clontech) or Fusion Red ((43 ...
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bioRxiv - Neuroscience 2020Quote: ... pN1-EGFP (Clontech) was used for visualisation of transfected cells ...
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bioRxiv - Cell Biology 2022Quote: ... EGFP-C1 (Clontech), mCherry-C1 (Clontech) ...
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bioRxiv - Developmental Biology 2024Quote: ... with eGFP (Clontech) and mKate2 (Evrogen ...
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bioRxiv - Neuroscience 2023Quote: ... eGFP plasmid (Clontech) at 5 ng/μL and pOCTmCherry at 1 μg/mL (Tradewell et al ...
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bioRxiv - Molecular Biology 2020Quote: ... and EGFP (632380, Clontech). Secondary antibodies were anti-mouse (ABIN6699027 ...
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bioRxiv - Developmental Biology 2022Quote: ... anti-EGFP (Clontech, 632569) and anti-β-actin (Santa Cruz ...
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bioRxiv - Biochemistry 2021Quote: ... anti-EGFP (#632381, Takara), anti-mCherry (#PA534974 ...
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bioRxiv - Developmental Biology 2023Quote: ... followed by insertion of a flexible linker72 at the C-terminus of EGFP using the InFusion system (Takara). α-tubulin CDS was amplified from a cDNA clone ...
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bioRxiv - Neuroscience 2020Quote: ... we replaced the EGFP fluorophore from pMH4-hsyn-ER-EGFP with DsRed2 (Clontech). For testing expression of MyoVa-mEmerald ...
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bioRxiv - Developmental Biology 2021Quote: Expression vectors for eGFP fusions were generated in eGFP N1 (Clontech, #6085-1) by subcloning of PCR fragments obtained using primers and template vectors shown in Supplemental Table S1 ...
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bioRxiv - Microbiology 2022Quote: ... US10 was cloned in frame with eGFP in the pCMS-eGFP vector (Clontech) using primers shown in Table 3 ...
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bioRxiv - Neuroscience 2023Quote: ... and then the coding sequence of eGFP (sequences extracted from pIRES2-EGFP, Clontech). Hβ4StrepTagII-nAChR-IRES-mTurquoise ...
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bioRxiv - Neuroscience 2020Quote: ... pN1-EGFP (Clontech, 6085-1) was used for cell visualisation.
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bioRxiv - Neuroscience 2020Quote: ... pN1-EGFP (Clontech, 6085-1) was used for cell visualisation.
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bioRxiv - Biochemistry 2021Quote: ... cloned in pIRES2-EGFP (Clontech), were previously described (42,43).
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bioRxiv - Cell Biology 2023Quote: ... elegans and EGFP-C1 (Clontech) for mammalian cells ...
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bioRxiv - Neuroscience 2023Quote: ... and eGFP (Clontech. CA, USA) in tsA201 cells using Lipofectamine 2000 (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... and pLVX-N3-EGFP (Clontech) that had been Gateway-adapted using the Gateway Vector Conversion kit (Invitrogen) ...
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bioRxiv - Cell Biology 2024Quote: ... EGFP from pEGFP-C1 (Clontech) was inserted into pLVX-IRES-Puro using the SnaBI and BamHI restriction sites.
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bioRxiv - Microbiology 2021Quote: ... complementary to the DENV 3’UTR at 65°C for 3 hrs using ExpressHyb hybridization buffer (Clontech). Autoradiographs were quantified using ImageQuant (GE Healthcare).
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bioRxiv - Microbiology 2020Quote: DNA encoding the eGFP sequence was PCR amplified out of the eGFP-N2 plasmid (Clontech) and subcloned via single-cut restriction digest into the corresponding UL25 plasmid harboring the cleavable His6-SUMO tag [(either UL25Δ50 (pED13 ...
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bioRxiv - Cell Biology 2020Quote: EGFP-actin and EGFP-CAAX (pEGFP-F, farnesylated) was purchased from Clontech (Mountain View, USA), and remaining constructs published previously as follows ...
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bioRxiv - Cell Biology 2021Quote: ... was constructed similarly by placing the HRAS-CAAX box motif on the C-terminus of EGFP in the pEGP-C1 vector (Clontech). The RFP-Myo10 construct was prepared by cloning mApple (from Addgene No ...
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bioRxiv - Microbiology 2022Quote: ... PreS2-LHBS sequence (deletion nucleotides 2-55) was cloned into the protein stability construct after the c-terminal of EGFP by In-Fusion cloning (Takara). The protein stability construct was a kind gift from Dr ...
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bioRxiv - Neuroscience 2023Quote: ... inserting an in-frame 15bp flexible linker sequence (encoding the amino acids GGGGA) followed by EGFP at the C-terminus using Infusion cloning (Clontech). For the Tg(NBT:ap2s1-gfp ...
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bioRxiv - Immunology 2021Quote: ... Fgf9 cDNA was cloned upstream of the IRES-eGfp-SV40 polyA cassette (pIRES2-eGfp vector, Clontech) in the pTRE2 vector (Clontech)49 ...
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bioRxiv - Cell Biology 2022Quote: ... TurboGFP was exchanged for eGFP by amplifying the eGFP sequence by PCR from peGFP-N1 (Clontech) using the following primers to introduce AgeI and NotI restriction sites ...
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bioRxiv - Molecular Biology 2020Quote: ... EGFP in pEGFP-C2 (Takara Bio) was replaced by the wild-type CARD14 gene obtained from pFN21AE3344 with N-terminal 3xFLAG tag peptides (DYKDHDGDYKDHDIDYKDDDDK ...
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bioRxiv - Neuroscience 2020Quote: Enhanced green fluorescent protein (EGFP, Clontech), Tag-blue fluorescent protein (Tag-BFP ...
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bioRxiv - Genetics 2020Quote: ... and ligated to N1-EGFP (Clontech) digested with KpnI and BamHI ...
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bioRxiv - Neuroscience 2024Quote: EGFP derived from pEGFP-C1 (Clontech) was cloned in-frame to the N-terminal domain of the mouse NFL gene (derived from pmNFL ...
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bioRxiv - Neuroscience 2023Quote: ... 5) cDNA coding eGFP protein (Clontech). 6 ...
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bioRxiv - Cell Biology 2024Quote: EGFP-Actin (Clontech cat #6116-1). Apple ...
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bioRxiv - Microbiology 2024Quote: ... EGFP- N1 was purchased from Clontech. pFB-Luc was purchased from Agilent.
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bioRxiv - Cell Biology 2020Quote: ... EGFP-Zdk1-NuMA-C was cloned into the CMV promoter plasmid mCherry-Zdk1-EB1-C (van Haren et al., 2018) as follows: mCherry was replaced by EGFP amplified from pEGFP-C1 (Clonetech, Takara Bio) and EB1-C was replaced by NuMA-C1701-2115 ...
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bioRxiv - Genomics 2020Quote: ... with a flag tag at the N terminal and an EGFP tag at the C terminal by the In-Fusion HD Cloning system (TaKaRa, 638909). After transfection of the U2OS cells with the pCMV-Tet3G Vector ...
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bioRxiv - Microbiology 2023Quote: ... pcDNA-EGFP-P2A-stop was constructed by amplifying the EGFP and P2A sequences from pEGFP-C1 (Clontech) using the primers shown in S-Table 3 and cloning it into the BamHI and EcoRI sites of pcDNA3.1(+ ...
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bioRxiv - Developmental Biology 2021Quote: ... was incubated with antibodies at 4°C for one hour: 3 µl (0.5 µg/µl) c-Myc monoclonal antibody (Cat. No. 631206, TaKaRa), or 1 µl (1.1 µg/µl ...
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bioRxiv - Cancer Biology 2021Quote: ... and the oligonucleotides were inserted into C terminal of ARL4C-EGFP or ARL4C-FLAG-HA using In-Fusion HD Cloning Kit (Takara Bio Inc.).
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bioRxiv - Neuroscience 2021Quote: ... The plasmid pIRESpuro3-EGFP was constructed by excising the EGFP open reading frame from pEGFP-C1 vector (Clontech) with NheI and BamHI digestion and insertion into the same restriction sites of the pIRESpuro3 vector (Clontech ...
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bioRxiv - Bioengineering 2023Quote: ... Plasmids coding EGFP-mutants were generated from EGFP-IRES-mCherry plasmid using TaKaRa MutanBEST Kit (TaKaRa, Kusatsu, Japan). pG5egfp reporter vector was modified from the pG5luc vector (GeneBank Accession Number AF264724 ...
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bioRxiv - Microbiology 2022Quote: ... The coding sequence of enhanced green fluorescent protein (eGFP) was amplified from plasmid eGFP-C1 (6084-1, Clontech) using primers eGFPN-F/eGFPN-R ...