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Citations for Takara Bio :
51 - 100 of 912 citations for pIEXBac c EGFP 3 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... Full-length of itga2 was inserted into the Sfi IA (5′-GGCCATTACGGCC-3′) and Sfi IB (3′-GGCCGCCTCGGCC-5′) sites of the “prey” pPR3-C vector (Clontech). Series of combinations of bait and prey constructs were cotransformed into the yeast strain NMY51 (Clontech) ...
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bioRxiv - Biochemistry 2022Quote: ... previously subcloned into pIRES2-EGFP vector (Clontech) using XhoI and EcoRI restriction enzyme sites (Yang et ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and subcloned into pCMS-EGFP vector (Clontech). The amplified fragments of Crbn ...
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Reduction of Spermine Synthase Suppresses Tau Accumulation Through Autophagy Modulation in TauopathybioRxiv - Neuroscience 2023Quote: ... and plasmids expressing EGFP (pEGFP-C1, Clontech) or Tau into SH-SY5Y cells (CRL-2266 ...
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bioRxiv - Neuroscience 2023Quote: The pIRES2-EGFP vector (Cat. #V11106; Clontech) was modified by enzymatic restriction to insert the Dlx5/6 promoter (Gift from G ...
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bioRxiv - Cell Biology 2024Quote: ... and subcloning into the EGFP-N1 (Clontech) plasmid using NheI/SalI restriction sites ...
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bioRxiv - Cell Biology 2024Quote: ... A bicistronic construct expressing human EPAC1b with a C-terminal His10 tag and SUMO3(Q89K) was constructed using the pIRES2-EGFP vector (Clontech Catalog no. 632435). The EPAC1-His10-IRES-SUMO3(Q89K ...
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bioRxiv - Cell Biology 2021Quote: ... pLL5.0-EGFP-HRasC20 was generated by insertion of EGFP-HRasC20 fragment which was amplified from pEGFP-F (#6074-1; Clontech) by PCR into pLL5.0 vector ...
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bioRxiv - Cancer Biology 2020Quote: ... overhangs (see table for primer sequences) and fused in frame at its carboxyl end via GA to eGFP in the vector eGFP-N3 (CLONTECH), to generate flZEB1-GFP ...
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bioRxiv - Cell Biology 2024Quote: ... both FRB-Rab6 ires Rab5-FKBP-EGFP and FRB-Mini-Giantin ires-Rabex5-FKBP-EGFP derive from the pIRESneo3 (Clontech-Takara), FRB and FKBP fragments were obtained by digestion ...
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bioRxiv - Neuroscience 2020Quote: ... the CMV-EGFP plasmid (pEGFP; Clontech, CA, USA) was used as a positive control ...
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bioRxiv - Cell Biology 2020Quote: ... EGFP was PCR-amplified from pEGFP-C2 (Clontech) and similarly cloned into the pRSET His-SS-TEV vector ...
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bioRxiv - Cell Biology 2023Quote: The respective cDNAs encoding EGFP (Clontech, 6084-1), SNAP-tag (New England BioLabs ...
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bioRxiv - Neuroscience 2022Quote: ... TagBFP-P2A-Pre-Sphere-SF-iGluSnFR and mCherry-P2A-Post-Sphere-SF-iGluSnFR sequences were inserted into the pAAV-hSyn-EGFP Vector by replacing the EGFP using Infusion (Takara Bio). AAV serotype 2 was amplified in HEK293T cells by transfecting pAAV.hSynapsin.TagBFP-P2A-Pre-Sphere-SF-iGluSnFR or pAAV.hSynapsin.mCherry-P2A-Post-Sphere-SF-iGluSnFR via the pRC2-mi342 and pHelper vectors ...
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bioRxiv - Cell Biology 2024Quote: ... both FRB-Rab6 ires Rab5-FKBP-EGFP and FRB-Mini-Giantin ires-Rabex5-FKBP-EGFP derive from the pIRESneo3 (Clontech-Takara), FRB and FKBP fragments were obtained by digestion ...
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bioRxiv - Cell Biology 2020Quote: The eGFP insert was amplified using pEGFP-N2 (Clontech) as a template and the DNA oligonucleotides oAH211 and oAH212 (untagged and N-terminal cloning ...
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bioRxiv - Microbiology 2021Quote: ... mouse monoclonal antibody against EGFP (Clontech, Mountain View, CA) was used for western blotting at a dilution of 1:1,000 and rabbit polyclonal antiserum reactive against protein kinase A (PKA ...
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bioRxiv - Neuroscience 2020Quote: ... Plasmids for the expression of eGFP (Clontech, Kyoto, Japan) were co-transfected in order to identify cells expressing the plasmids ...
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bioRxiv - Neuroscience 2020Quote: ... pN1-EGFP or pC1-tdTomato (Clontech; Mountain View, CA) were mixed with AMPAR-expressing plasmids at a base pair ratio of 1:7 ...
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bioRxiv - Cell Biology 2020Quote: The eGFP-C1 plasmid (kanamycin resistant; Clontech; 4731 bp) was used to generate N-terminal GFP-tagged Rab46 mutants ...
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bioRxiv - Cell Biology 2021Quote: ... a plasmid expressing enhanced green fluorescent protein (EGFP) (Clontech), or in another series of experiments with pVSV-G ...
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bioRxiv - Cell Biology 2020Quote: ... eGFP was PCR-amplified from pEGFPN1 (Takara Bio USA) and inserted using Gibson assembly into a NotI/BamHI-digested pACT (Table 1) ...
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bioRxiv - Molecular Biology 2021Quote: ... The EGFP ORF was amplified from pEGFP-N1 (Clontech). The ORFs were cloned into pcDNA3.1(+ ...
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bioRxiv - Microbiology 2020Quote: ... mouse monoclonal antibody against EGFP (Clontech, Mountain View, CA) was used for Western blotting at a dilution of 1:1,000 ...
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bioRxiv - Cell Biology 2020Quote: ... mTomm70A-EGFP was then transferred into pQCXIN (Clontech #631514) to generate pLenti-mTomm70A-EGFP ...
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bioRxiv - Microbiology 2021Quote: ... EGFP tagged construct was generated in pEGFP-N1 (Clontech).
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bioRxiv - Cell Biology 2022Quote: ... PtK2 cells were transfected with eGFP-α-tubulin (Clontech) using Viafect (Promega ...
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bioRxiv - Neuroscience 2024Quote: ... were coated with 15 µg of EGFP-N1 (Clontech), or 10 µg of tDimer-dsRed together with 16 µg SEP-GluA224 ...
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bioRxiv - Physiology 2024Quote: ... and the pCMV– EGFP–N3 plasmid was from Clontech, Inc.
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bioRxiv - Biochemistry 2024Quote: ... The PCR product was cloned into EGFP-C3 (Clontech). Primers for mutagenesis were designed using NEBaseChanger (sequences shown in table S1 ...
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bioRxiv - Bioengineering 2024Quote: ... The IRES sequence was derived from pIRES2-EGFP (Clontech). Other sequences were derived from public databases ...
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bioRxiv - Cell Biology 2020Quote: The GST-Bbs5 was expressed for 3 h at 27 °C in Escherichia coliBL21(DE3) (Takara) transformed with pGEX-4T-2-Bbs5-WT in the presence of 0.1 mM isopropyl-β-D-thiogalactopyranoside (Takara) ...
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bioRxiv - Neuroscience 2021Quote: Enhanced GFP (eGFP) was expressed from the pEGFP-N1 (Clontech). Homer1c-DsRed and Homer1c-GFP plasmids encoding for Homer1c fused with DsRed and GFP at the N terminus ...
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bioRxiv - Neuroscience 2020Quote: ... or with EGFP (Control) using a CalPhos Transfection Kit (Takara) at DIV10 and immunostained at DIV14 ...
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bioRxiv - Cell Biology 2020Quote: ... EGFP-tagged human β-actin (Clontech, Mountain View, CA, USA) was used ...
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bioRxiv - Neuroscience 2020Quote: ... pIRES2-eGFP (referred to as “empty vector” or “control”, Clontech); EphA7-FL-HA (“EphA7-FL-HA”) ...
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bioRxiv - Cancer Biology 2021Quote: The eGFP coding sequence from the pEGFP-C2 vector (Clontech), with or without the coding sequence of wild-type human TRPV2 (12 ...
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bioRxiv - Cell Biology 2023Quote: ... the EGFP sequence was PCR amplified from pEGFP-N1 (Clontech), and cloned in the pAIB-CAG lentiviral vector (a gift from Claudio Ballabio ...
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bioRxiv - Neuroscience 2023Quote: ... a Living Colors® EGFP mouse monoclonal antibody (632569, Clontech), or a mouse monoclonal anti-actin antibody (clone AC-40 ...
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bioRxiv - Cell Biology 2020Quote: The DNA constructs expressing EGFP- or mCherry-tubulin were from Takara Bio ...
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bioRxiv - Cell Biology 2020Quote: ... the EGFP gene was excised from pEGFP-N1 (Takara Clontech, France) with flanking EcoRI/BsrGI sites and replaced with the mCherry gene ...
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bioRxiv - Cancer Biology 2021Quote: ... the GFP-expressing bicistronic reporter construct pIRES2-EGFP (BD Biosciences Clontech) was used ...
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bioRxiv - Cell Biology 2021Quote: ... The EGFP coding sequence was amplified from pEGFP-N1 vector (Clontech) with the following primers ...
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bioRxiv - Cell Biology 2020Quote: ... along with eGFP PCR-amplified from pEGFP-N1 (Takara Bio USA), into the BamHI site of pACT (Table 1).
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bioRxiv - Molecular Biology 2020Quote: ... the EGFP ORF was PCR amplified from the pEGFPN1 vector (Clontech) with appended 5′ AfeI and 3′ EcoRI restriction sites ...
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bioRxiv - Immunology 2021Quote: ... Full length of mLIGHT was cloned into pIRES2-EGFP vector (Clontech), which contains a subsequent IRES (Internal Ribosome Entry Site ...
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bioRxiv - Neuroscience 2021Quote: ... The enhanced green fluorescent protein (EGFP) vector was obtained from Clontech Europe ...
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bioRxiv - Cell Biology 2020Quote: ... The EGFP sequence was amplified by PCR using pEGFP-N1 (Clontech) as a template ...
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bioRxiv - Cell Biology 2020Quote: ... the EGFP gene was excised from pEGFP-N1 (Takara Clontech, France) with flanking EcoRI/BsrGI sites and replaced with the mCherry gene ...
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bioRxiv - Bioengineering 2022Quote: ... EGFP was detected using the JL-8 mouse monoclonal antibody (Clontech). For IHC ...