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Citations for Takara Bio :
101 - 150 of 6347 citations for Rat Starch Binding Domain Containing Protein 1 STBD1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2023Quote: ... sSH2 domains were immediately purified by the N-terminal His6 tag with TALON® resin (Takara Bio) using a gravity column (detailed purification method in the Supporting Methods 1) ...
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bioRxiv - Neuroscience 2023Quote: ... Red Fluorescent Protein Antibody (DsRed, 1:500, Clontech). The brain sections were incubated with antibodies in blocking solution at 4°C temperature overnight ...
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bioRxiv - Biophysics 2020Quote: pDNA_HaloTag/SNAP-tag/GFP vectors encoding each target protein (see Note 1),which were inserted by In-Fusion HD Cloning Kit (Clontech) or Seamless Ligation Cloning Extract (SLiCE ...
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bioRxiv - Biophysics 2020Quote: ... The complex was then purified by binding on Talon resin (Clontech) and eluted in 150 mM imidazole ...
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bioRxiv - Biophysics 2024Quote: ... The supernatant was then diluted 1:5 to improve binding efficiency and incubated overnight on the rotisserie at 4°C with TALON Cobalt Resin (Takara Bio). The next day the resin was washed with 10 column volumes of Membrane Buffer 1 ...
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bioRxiv - Biophysics 2024Quote: ... The supernatant was then diluted 1:4 to improve binding efficiency and incubated overnight on the rotisserie at 4°C with TALON Cobalt Resin (Takara Bio). The next day the resin was washed with 10 column volumes of Membrane Buffer 2 and 10 column volumes of Membrane Buffer 2 with 20 mM imidazole before elution with Membrane Buffer 2 with 300 mM imidazole ...
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bioRxiv - Developmental Biology 2022Quote: ... rat anti-E-Cadherin (TAKARA; M110), rat anti-Endomucin (Santa Cruz ...
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bioRxiv - Pathology 2022Quote: ... Rat anti Mouse OCN (Takara, M188); Rabbit anti Mouse MGST1 (Abcam ...
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bioRxiv - Cell Biology 2021Quote: ... Protein concentrations were determined via the Bradford method using the TaKaRa Bradford Protein Assay Kit (Takara Bio, T9310A).
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bioRxiv - Molecular Biology 2021Quote: ... we performed ELISA against HIV p24 (TaKaRa Clontech #632200) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... we performed ELISA against HIV p24 (TaKaRa Clontech #632200) according to manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... and titer quantified using p24 ELISA antigen assay (Takara). MOI=5 was used to transduce 1x1097 EndoC-βH3 cells in culture media without pen/strep and puromycin.
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bioRxiv - Developmental Biology 2023Quote: ... The following antibodies were used: rabbit or rat anti-dsRed (1:200, Clontech 632496 or 5F8 1:400, Chromotek 5f8-100); chicken anti-GFP (1:800 ...
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bioRxiv - Microbiology 2021Quote: ... and 1 ng of a luciferase (luc2) containing plasmid (pGL4.26, Clontech) was added to buffer AL prior to lysis ...
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bioRxiv - Developmental Biology 2019Quote: ... 5μl of RT mix containing 1× SMART First Strand Buffer (Clontech), 2 mM dithiothreitol (Clontech) ...
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bioRxiv - Physiology 2022Quote: ... We used the Bradford Protein Assay Kit (TAKARA, T9310A, Shiga, Japan).
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bioRxiv - Cell Biology 2024Quote: ... The protein concentration was determined by using Bradford assay kit (Takara). For the vehicle control and inhibitor-treated or 5 ...
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A GID E3 ligase assembly ubiquitinates an Rsp5 E3 adaptor and regulates plasma membrane transportersbioRxiv - Cell Biology 2021Quote: ... pGADCg- and pGBKCg-based plasmids containing the indicated protein fusions were transformed into the yeast strain Y2HGOLD (Takara Bio), and double transformants were selected by growth on SD media lacking leucine and tryptophan ...
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bioRxiv - Molecular Biology 2021Quote: ... was co-transformed with GAL4 DNA-BD-fused IPMK and a human brain cDNA activation domain (AD) library (Clontech). Two different reporter genes (HIS3 and ADE2 ...
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bioRxiv - Molecular Biology 2021Quote: ... Coding regions of the heavy-chain variable domains were amplified using LA Taq polymerase (TAKARA Bio Inc., Shiga, Japan) with two PAGE-purified primers (CALL001 ...
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bioRxiv - Developmental Biology 2021Quote: ... and as a prey to the activation domain of the GAL4 transcription factor (GAL4-AD) in plasmid pACT2 (Clontech). To monitor the induction of a HIS3 reporter gene by complexes of bait and prey fusion proteins ...
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bioRxiv - Immunology 2021Quote: ... His-tagged NTD domain constructs were purified from clarified supernatants using 2 ml of cobalt resin (Takara Bio TALON), washing with 50 column volumes of 20 mM HEPES-HCl pH 8.0 and 150 mM NaCl and eluted with 600 mM imidazole ...
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bioRxiv - Microbiology 2020Quote: ... ramulus DSM 15684 were cloned without signal peptide or transmembrane domain from genomic DNA using In-Fusion cloning (Takara) and the primers in Table S8 into the EcoRI and Ncol restriction sites of the corresponding plasmids ...
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bioRxiv - Plant Biology 2023Quote: ... fused with GAL4 activation domain was amplified and subcloned into the prey vector pGADT7 (Clontech Laboratories, Inc., Kusatsu, Japan). Competent yeast cells were prepared according to the Clontech Yeast protocols handbook using the Y1H Gold yeast strain ...
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bioRxiv - Immunology 2021Quote: ... containing 2 μl lysis buffer per well (1:20 RNase inhibitor (Clontech) in 0.2% (v/v ...
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bioRxiv - Cell Biology 2023Quote: ... pH 7.4) and the protein contents of the cell lysate were quantified using a BCA Protein Assay Kit (TAKARA, T9300A) following the manufacturer’s protocol (Song et al. ...
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bioRxiv - Cell Biology 2023Quote: ... Supernatants were collected and mixed with Laemmli buffer after measuring the protein concentration using a TaKaRa BCA Protein Assay Kit (TaKaRa). Samples were subjected to SDS-polyacrylamide gel electrophoresis and electrotransferred onto polyvinylidene difluoride membranes ...
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bioRxiv - Molecular Biology 2024Quote: ... 4.9 μg DNA was transfected per dish containing a 1:1 molar ratio of pmCherry-N1 (Clontech) and of the pUC18-GFP or linear GFP substrate ...
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bioRxiv - Neuroscience 2023Quote: ... Green Fluorescent Protein (GFP) antibody (JL-8, 1:500, Clontech), Red Fluorescent Protein Antibody (DsRed ...
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bioRxiv - Molecular Biology 2019Quote: A DNA fragment containing CDS of each Hero protein and C-terminal FLAG and His tags was inserted into pCold I (Takara) by NEBuilder HiFi DNA Assembly Master Mix (NEB) ...
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bioRxiv - Biophysics 2020Quote: Orai1-CAD-CAD-GFP constructs were designed by cloning Orai1 and STIM1-CAD domains (aa344-449) into peYFP-N1 (Clontech). Linker regions between Orai1 and CAD (Orai1-linker-1-CAD-linker-2-CAD-YFP ...
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bioRxiv - Cell Biology 2022Quote: ... the PCDH15-CD2 isoform cytoplasmic domain was cloned using the HindIII and BamHI restriction sites of the pmCherry-C2 vector (Clontech), resulting in soluble protein tagged with cherry at its N-terminus.
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bioRxiv - Microbiology 2020Quote: ... cDNA library derived from soybean hypocotyl and roots RNA were constructed to pGADT7 plasmid fused with GAL4 activation domain (AD) by Clontech company ...
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bioRxiv - Plant Biology 2022Quote: ... GID1b and GID1c cDNAs inserted into pDONR221 were fused to the activation domain GAL4 (AD) after recombination into pGADT7 (Clontech).
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bioRxiv - Microbiology 2021Quote: ... Forward and reverse primers carrying the above restriction sites were designed to amplify a 3kb fragment from Diaph2 MGC human cDNA clone (Dharmacon) and the amplicon encompassing the entire cDNA sequence minus the DAD domain was cloned into pLVX-mCherry-N1 (Clontech) to generate lentiviral expression vector pLVXdeltaDADmcherry ...
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bioRxiv - Plant Biology 2020Quote: The transformation of each plasmid for the respective domain configurations was performed as described by Gietz and Schiestl (2007) using Saccharomyces cerevisiae strain HF7C (Clontech).
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bioRxiv - Plant Biology 2021Quote: ... fragments containing a stop codon were recombined into Gateway-compatible versions of the GAL4 DNA BD vector pGBT-9 and the activation domain vector pGAD424 (Clontech) using L/R-clonase (ThermoFisher) ...
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bioRxiv - Genetics 2019Quote: ... The entire open reading frame for the protein Reg1 and its derivative Reg1-P231L was expressed as a fusion to the Gal4 activation domain in the plasmid pACT2 (Clontech).
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bioRxiv - Microbiology 2023Quote: ... Fragments encoding the CD45 extracellular domain (ECD) and transmembrane region were amplified by PCR and cloned into pEGFP-N1 (Clontech) using the EcoRI and BamHI restriction sites ...
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bioRxiv - Immunology 2022Quote: ... Mate & Plate™ cDNA Library in a pGADT7 vector with the GAL4 DNA-activation domain (AD) and pre-transformed into yeast strain Y187 was acquired from Takara Bio ...
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bioRxiv - Plant Biology 2023Quote: ... The LexA fusion constructs (bait) and the activation domain fusion constructs (prey) were co-transformed into yeast strain EGY48[p8op-lacZ] (CLONTECH). Clones containing both constructs were selected on medium lacking His ...
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bioRxiv - Plant Biology 2023Quote: ... pGADT7 plasmids encoding the effector domain of different ZiF were co-transformed into chemically competent Y2HGold cells (Takara Bio, USA) with a pGBKT7 plasmid encoding OsExo70F3 in using a Frozen-EZ Yeast Transformation Kit (Zymo research).
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bioRxiv - Molecular Biology 2024Quote: The RBD-DsRed plasmid was created by PCR cloning the HB domain of RNAse H1 into the pDsRed-Express-C1 vector (Clontech), following the previously described method [32].
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bioRxiv - Plant Biology 2024Quote: ... the full-length ORF of PpGL2 was fused with the GAL4 transcriptional activation domain in the pGADT7 vector (Clontech, Japan), and then co-transformed with the pHIS2 cis reporter vector (containing triple tandem CAAATTG repeat sequence ...
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bioRxiv - Genetics 2019Quote: 3000 quiescent satellite cells were lysed after FACS by sorting directly into a 0.2ml tube containing 1 μl SMART-Seq Reaction Buffer (95% SMART-Seq 10x lysis buffer containing 5% SMART-Seq RNAse Inhibitor, Takara Bioscience, Cat. 634890) in 8μl ddH20 ...
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bioRxiv - Animal Behavior and Cognition 2019Quote: RNA was extracted from the granulation tissue of the rats using a NucleoSpin® RNA II kit (Takara Bio, Otsu, Japan). Each sample was harvested from the right quarter of a wound with approximately 5-mm margins without the cranial bone ...
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bioRxiv - Neuroscience 2023Quote: ... Rat Shank3 deletion constructs in pmRFP-N3 (Clontech) were described before (23) ...
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bioRxiv - Biochemistry 2021Quote: ... The recombinant proteins were purified with His60 Ni Gravity Column Purification Kit (Clontech) following a manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: The cDNA coding sequences of the first and second cytoplasmic loop domain of human TMEM39A were cloned into the pGBKT7 vector and screened against a normalized universal human cDNA library (Clontech, 630481), following instruction of the Matchmaker® Gold Yeast Two-Hybrid System (Clontech ...
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bioRxiv - Plant Biology 2020Quote: ... and pGBKT7 (DNA-binding domain vector) by either restriction enzyme-based cloning or In-Fusion HD Cloning method (638910In-Fusion® HD Cloning Plus Takara). Primer sequences used to generate Y2H and Y3H constructs are listed in Supplemental Table 4 ...