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Citations for Takara Bio :
351 - 400 of 6347 citations for Rat Starch Binding Domain Containing Protein 1 STBD1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2022Quote: ... containing both commercially available human genomic DNA (100ng/µl; ClonTech, #636401) and mutant DNA from either a cell line (c.1620C>A ...
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bioRxiv - Developmental Biology 2024Quote: ... containing 10% tetracycline-free fetal calf serum (FCS) (TaKaRa Bio, 631106), 2 mM L-Glutamine (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: Expression plasmids for fusion proteins of GFP and tardigrade proteins were constructed by Gibson assembly into pEGFP-N1 (Clontech) of the tardigrade cDNA (obtained by gene synthesis from Integrated DNA ...
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bioRxiv - Molecular Biology 2020Quote: A codon-optimized synthetic gene corresponding to the full length nucleocapsid protein (Thermo GeneArt, Regensburg, Germany) was cloned into a modified pET28a vector using the In-Fusion cloning kit (Takara Bio Saint-Germain-en-Laye, France). The resulting plasmid encoded for an N-terminal His6 tag followed by thrombin and tobacco etch virus cleavage sequences upstream of the full-length nucleocapsid protein sequence ...
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bioRxiv - Evolutionary Biology 2022Quote: ... with 0.1 µL of Taq Polymerase 5 U.µL-1 (TaKaRa Ex Taq® Kit MgCl2 Free Buffer) (TaKaRa Ex Taq, TaKaRa Bio, Shiga, Japan), 2.5 µL PCR Buffer 10X ...
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bioRxiv - Neuroscience 2022Quote: ... slices were incubated in a blocking buffer containing the primary antibodies rabbit anti-mCherry (1:1500; cat. No. 632496, Takara Bio USA, Inc., Mountain View, CA, USA) and chicken anti-GFP (1:1500 ...
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bioRxiv - Cell Biology 2019Quote: ... and both proteins were separated from each other and from a protein encoded by blasticidin resistance gene (cloned from pQCXIB #631516, Clontech) by self-cleavage peptide P2A ...
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Osh6 requires Ist2 for localization to the ER-PM contacts and efficient phosphatidylserine transportbioRxiv - Cell Biology 2020Quote: For testing protein-protein interactions Matchmaker™ GAL4 Two-Hybrid System 3 was used according to the manufacturer’s instructions (Clontech). Briefly ...
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bioRxiv - Bioengineering 2021Quote: ... 1 μg RNA was reverse transcribed using the PrimeScript RT reagent Kit with gDNA Eraser (Takara). The cDNA was then amplified for further analyses using deep sequencing on Illumina NextSeq platform ...
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bioRxiv - Bioengineering 2021Quote: ... RNA (1 μg) was reverse transcribed using the Perfect real-time cDNA reverse transcription kit (TaKaRa). Quantitative PCR was performed with 1 μg of cDNA per sample per target gene using AceQ qPCR SYBR Green master mix (Vazyme ...
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bioRxiv - Immunology 2021Quote: ... cDNA was synthesized from 1 μg of total RNA using an RNA reverse transcriptase kit (Takara). Real-time qPCR was performed using the ABI PRISM 7000 Sequence Detection System (Applied Biosystems) ...
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bioRxiv - Cell Biology 2022Quote: ... Total RNA (1 μg) was reversed to cDNA with PrimeScript™ RT reagent Kit (Takara, RR047Q), which was performed according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... cDNA was prepared with 1 µg RNA and synthesized using the Primescript RT kit (Takara Inc.). qPCR assays were performed on the StepOnePlusTM Real-time PCR System (Applied Biosystems ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 ng of total RNA was reverse transcribed with the cDNA synthesis kit (Takara Bio Inc.) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... 1 μg total RNA was reverse transcribed into cDNA with the SMARTer RACE 5’ Kit (Clontech). PCR was performed using primer GSP-HO1 and the Universal Primer Mix (UPM ...
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bioRxiv - Genetics 2019Quote: ... liver and kidney), mouse tissues (brain, liver, heart, testis and kidney) and rat tissues (brain, liver and kidney) were purchased from Takara Bio Inc.
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bioRxiv - Neuroscience 2023Quote: ... Full-length and truncated Cntn1 was PCR amplified from rat contactin-myc 34 and ligated together using an In-Fusion Snap Assembly Master Mix (Takara). All DNA constructs were verified by sequencing (Genewiz and plasmidsaurus).
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bioRxiv - Genomics 2020Quote: ... CAS9 protein was purchased from Clontech (Cat # 632641). For injection ...
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bioRxiv - Biochemistry 2021Quote: ... Protein was purified using amylose-agarose resin (Clontech) and eluted in 10 mM maltose ...
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bioRxiv - Biochemistry 2020Quote: ... Protein concentration was determined by Bradford assay (TAKARA). Nickel affinity purification of 10His-SUMO1T95R conjugates was carried out with Ni-NTA agarose beads (Qiagen) ...
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bioRxiv - Biochemistry 2022Quote: ... Proteins were bound to TALON IMAC resin (Clontech) overnight at 4 °C ...
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bioRxiv - Microbiology 2021Quote: ... and protein purified using TALON resin (Takara Bio) using standard protocols in 50 mM phosphate buffer pH7.4 containing 300mM NaCl.
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bioRxiv - Molecular Biology 2022Quote: ... The protein was purified by TALON affinity (Clontech), HiTrap-Q anion-exchange (Cytiva ...
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bioRxiv - Microbiology 2022Quote: ... proteins were purified using Talon resin (Takara Bio) by resuspending the cell pellet in lysis buffer (50 mM phosphate buffer and 300 mM NaCl ...
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bioRxiv - Evolutionary Biology 2023Quote: ... recombinant protein (vector pColdTM TF-DNA, Takara Clontech) to use as the antigen ...
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bioRxiv - Evolutionary Biology 2023Quote: ... recombinant protein (vector pColdTM TF-DNA, Takara Clontech) to use as the antigen ...
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bioRxiv - Neuroscience 2023Quote: ... sagittal sections (50 µm) of the cerebellum were cut and incubated with antibodies against the reporter protein mCherry (Clontech 632543, RRID: AB_2307319, 1:500) and against the P-cell marker calbindin (Swant CB38 ...
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bioRxiv - Molecular Biology 2020Quote: ... A polymerase chain reaction (PCR) master mix containing ExTaq DNA polymerase (Clontech), ExTaq buffer (Clontech) ...
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bioRxiv - Cell Biology 2019Quote: ... Nck1 was cloned into pEGFP vector containing EGFP with A206K mutation (Clontech). Engineered Nck proteins were generated as repeats of the second SH3 domain (SH3b ...
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bioRxiv - Microbiology 2021Quote: ... into 48-well plates containing 2.6 µl of 1x lysis buffer (Takara) supplemented with 0.01 µl of RNase inhibitor (40 U/µl ...
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bioRxiv - Cell Biology 2022Quote: ... the medium was exchanged with primate ES Cell Medium containing doxycycline (TaKaRa) (1.0 μg/mL) ...
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bioRxiv - Immunology 2020Quote: ... The probe-containing vector was then transformed into Stellar Competent Cells (Takara) via manufacturer protocol ...
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bioRxiv - Evolutionary Biology 2022Quote: ... both containing appropriated overlapping regions for INFusion® cloning (TaKaRa Bio Inc) into the target vector pET28a(+) ...
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bioRxiv - Biochemistry 2022Quote: ... A reaction volume of 25 μl containing 1XPrimeStar Max DNA Polymerase (Takara), 300 nM of forward and reverse primers and 100 ng of DNA ...
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bioRxiv - Cell Biology 2021Quote: ... A polymerase chain reaction (PCR) master mix containing ExTaq DNA polymerase (Clontech), ExTaq buffer (Clontech) ...
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bioRxiv - Molecular Biology 2022Quote: ... 50 mM maleic acid buffer pH 5.5) containing 100 mg Yatalase (Takara) and 100 mg Lysing Enzymes from Trichoderma harzianum (Sigma ...
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bioRxiv - Cell Biology 2024Quote: ... rat cerebral cortexes or fly brains using TRIzol and the reverse transcription was performed with PrimeScript™ RT Master Mix (Takara) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... cDNA synthesized from 1 μg total RNA using PrimeScript™ RT reagent Kit with gDNA Eraser (Takara). Real-time PCR was performed using TB Green® Premix Ex Taq™ II (Tli RNaseH Plus ...
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bioRxiv - Genomics 2019Quote: ... 1 ng of total RNA was amplified using the SMARter Ultra Low amplification kit (Clontech, Mountain, CA) with PCR amplification to generate sufficient cDNA for library construction ...
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bioRxiv - Physiology 2021Quote: ... 1 μg RNA was used to synthesize first strand of cDNA using PrimeScriptTM RT-PCR Kit (TaKaRa). qPCR was conducted using PrimeScriptTMRT Master Mix (TaKaRa ...
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bioRxiv - Immunology 2022Quote: ... 1 μg of total RNA was used to synthesize cDNA with PrimeScript RT reagent kit (RR047A, Takara) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... RNA (1 ng) was amplified using SMARTer library Ultra Low cDNA v4 kit (Takara Bio, Mountainview, CA). Sequencing cDNA libraries were preparing using a NexteraXT DNA library prep kit (Illumina ...
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bioRxiv - Cancer Biology 2022Quote: ... cDNA was generated from 1 ng of input RNA using the SMART-Seq HT Kit (Takara 634455) at half reaction volume followed by Nextera XT (Illumina FC-131-1024 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... with 0.1 µL of Taq Polymerase 5 U.µL-1 (TaKaRa Ex Taq® Kit MgCl2 Free Buffer) (TaKaRa Ex Taq ...
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bioRxiv - Molecular Biology 2022Quote: cDNA was synthesized with 1 μg of total RNA using the Prime ScriptTM RT Reagent Kit (Takara) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... 1 μg of total RNA was reversely transcribed into cDNA using the PimeScript RT-PCR kit (TAKARA) and analyzed by qPCR on LightCycler96 system (Roche ...
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bioRxiv - Molecular Biology 2023Quote: ... Total RNA (1 μg) was reverse transcribed with the PrimeScript™RT Reagent Kit (Takara Bio Inc.) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... a plasmid expressing enhanced green fluorescent protein (EGFP) (Clontech), or in another series of experiments with pVSV-G ...
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bioRxiv - Biophysics 2020Quote: ... The protein was initially purified using Talon resin (Clontech) with a linear gradient of 50 to 200 mM imidazole ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were purified using TALON Metal Affinity Resin (Clontech) and dialyzed overnight against PBS buffer ...