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Citations for Takara Bio :
1301 - 1348 of 1348 citations for pVectOZ CAT Transfection control since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... we linearized the pnCS plasmid with NdeI/NheI and employed seamless cloning (In-Fusion HD Cloning Plus Kit from Takara Bio, Cat. # 638910) with the following primers ...
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bioRxiv - Cell Biology 2020Quote: A lentiviral plasmid encoding a transcriptional reporter for CREB activity was generated by PCR amplification of a 2xCRE promoter-driven GFP N-terminally tagged with the ProteoTuner destabilization domain (CRE-DD-GFP, Takara Bio, Cat #631085) and Gibson cloning into the FUGW lenti-vector backbone (Addgene ...
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bioRxiv - Cancer Biology 2021Quote: ... CRISPR/Cas9 plasmid was virally transduced into cells using Lenti-X Packaging Single Shots (VSV-G) using manufacturer’s instructions (Clontech, California, USA, Cat #631275). See Table S3 for list of all gRNAs utilized.
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bioRxiv - Cancer Biology 2021Quote: Total RNA (1 μg) was reverse transcribed to cDNA using the PrimeScriptTM RT reagent kit with gDNA Eraser (Cat#: RR047A, TaKaRa Bio, Shiga, Japan). qRT–PCR was performed using a LightCycler 480 II instrument (Roche Molecular Systems ...
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bioRxiv - Molecular Biology 2021Quote: SARS-CoV-2 N and E genes were transcribed from the pBluescript-N and pUC57-E plasmids by adding a T7 promoter via PCR using Premix Taq (Cat. No. R004A, TAKARA, Shuzo, Shiga, Japan). The crRNA templates were amplified from a pUC57-T7-crRNA (Supplementary Table S10 ...
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bioRxiv - Microbiology 2022Quote: ... we amplified the gene (ORF2) encoding the VP1 sequence of the HuNoV Sydney strain by PrimeStar HS DNA polymerase (Takara Bio, cat. #r010a) and replaced it with the sequence encoding GFP in the pT7-RRV-NSP3-GFP constructed previously (12 ...
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bioRxiv - Plant Biology 2022Quote: ... were amplified using a set of primers with Gateway attB sites (supplementary Table S2) and cloned into the pABAi vector (Cat. No. 630491, Takara Bio USA, Inc.). The MaMYBPA1 and MaMYBPA2 coding sequences were cloned into the pGADT7-GW vector (Clontech ...
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bioRxiv - Molecular Biology 2022Quote: ... Each sample was then incubated at 37°C for 30 min with 20 U of mRNA-Interferase™ MazF enzyme (Takara, Cat# 2415A) in a 10 μL volume reaction containing 1X MazF reaction buffer ...
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bioRxiv - Plant Biology 2022Quote: ... and impact of SAID1/SAID2 on SE interaction with other proteins was examined on SD-His/-Leu/-Met/-Trp quadruple dropout medium (Clontech, Cat. No. 630429) supplemented with 5 mM 3-amino-1,2,4-triazole (3-AT ...
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bioRxiv - Physiology 2022Quote: ... The rat IGF1 coding sequence was then inserted into the linearized scAAV-CMV plasmid using In-Fusion cloning (Takara Bio; Cat. No. 639650). The resulting plasmids for scAAV-CMV-GFP and scAAV-CMV-IGF1 were packaged using AAV2/9 serotype by Vector Biolabs (Malvern ...
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bioRxiv - Biophysics 2022Quote: ... were mixed to a final volume of 150 µL and incubated at room temperature for 30 min before adding to the Lenti-X™ 293T Cell Line (Takara, Cat. #: 632180). Media (10% FBS ...
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bioRxiv - Biophysics 2022Quote: ... containing virus was collected after 48 hrs and concentrated by 50-fold following the protocol of Lenti-X™ Concentrator (Takara, Cat. #: 631232). The concentrated virus in 40uL volume was added to HEK293T cells plated several hours ahead started with 80,000 cells in one well of a 24-well plate ...
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bioRxiv - Biochemistry 2024Quote: ... Sub-library specific primers were then used to amplify 1 ul of cDNA template for 24 cycles using Advantage 2 PCR Mix (Takara Bio, Cat. 639206). Finally ...
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bioRxiv - Immunology 2024Quote: ... RNA was reverse transcribed and cDNA amplified by in vitro transcription with the TaKaRa SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (TaKaRa, Cat. No. 634889) or a low input method using NEB Next® Ultra RNA Library Prep Kit for Illumina® (NEB ...
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bioRxiv - Developmental Biology 2023Quote: ... high-quality cDNA was generated from the total RNA using the SMART-Seq v4 Ultra Low Input RNA Kit (Takara Bio, Cat. #634889), and RNA-seq libraries were generated using the SMARTer ThruPLEX DNA-Seq Kit (Takara Bio ...
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bioRxiv - Cancer Biology 2024Quote: ... pHTN HaloTag® CMV-neo and pHTC HaloTag® CMV-neo were digested with restriction enzymes and subsequently ligated (Takara, cat n°6023). Primers and corresponding restriction digests can be found in Table S4 ...
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bioRxiv - Immunology 2023Quote: ... RNA isolation and cDNA amplification was performed using either Smart-Seq® v4 Ultra® Low Input RNA Kit (Takara Clontech, cat# 635015) or NORGEN BIOTEK (cat# 51800) ...
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bioRxiv - Immunology 2023Quote: ... RNA isolation and cDNA amplification was performed using either Smart-Seq® v4 Ultra® Low Input RNA Kit (Takara Clontech, cat# 635015) or NORGEN BIOTEK (cat# 51800) ...
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bioRxiv - Microbiology 2023Quote: ... or the BamHI/MluI site of pWPI-ACE2-zeo (for ACE2 expression plasmids)43 with 3×FLAG-tag at the C-terminus using In-Fusion® HD Cloning Kit (Takara, Cat# Z9650N). Nucleotide sequences were determined by DNA sequencing services (Eurofins) ...
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bioRxiv - Neuroscience 2023Quote: ... then cDNA was synthesized using the SMART-Seq® v4 Ultra® Low Input RNA kit (Takara Bio USA Inc. Cat No. 634888). Libraries were prepared using the NexteraTM DNA Flex Library Prep (Illumina Cat No ...
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bioRxiv - Developmental Biology 2023Quote: DNA template for RNA synthesis was created using the forward and reverse primers listed below with GXL prime star PrimeSTAR® GXL DNA Polymerase (Takara Cat# R050A). mMESSAGE mMACHINE™ T7 ULTRA Transcription Kit (ThermoFisher Cat# AM1345 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The first-strand cDNAs of mRNA and mature miRNA were synthesized from 500 ng of total RNA using PrimeScript™ RT reagent kit with gDNA Eraser (Cat# RR047A, Takara, Kyoto, Japan) and miRcute Plus miRNA First-Strand cDNA Synthesis Kit (Cat# KR211 ...
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bioRxiv - Cell Biology 2023Quote: ... Sequences encoding EGFP and murine RPL10a was obtained and fused according to Heiman et al(23) using In-Fusion HD (Takara Bio cat# 011614). The EGFP-L10a fusion construct was incorporated downstream of CreERT2(67 ...
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bioRxiv - Cell Biology 2023Quote: ... The hiPSC lines were differentiated into human pluripotent stem cell derived hepatocytes (hiPSC-HEP) by using the Cellartis iPS Cell to Hepatocyte Differentiation System (Cat. No. Y30055, Takara Bio Europe AB) according to the manufacturer’s recommendations.
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bioRxiv - Plant Biology 2023Quote: The 5’ and 3’ experiments were conducted with the use of SMARTer® RACE cDNA Amplification Kit (Takara Bio, United States, Cat# 634860) and Advantage Polymerase as described by Kruszka et al ...
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bioRxiv - Plant Biology 2023Quote: ... One microgram of total RNA was used for complementary DNA (cDNA) synthesis by PrimeScript first strand cDNA synthesis kit (TAKARA-Bio, Cat# 6110) using an oligo(dT ...
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bioRxiv - Molecular Biology 2023Quote: ... with its native promotor and was subcloned into a modified pACYC vector using In-Fusion cloning strategy (In-Fusion® Snap Assembly Master Mix; TaKaRa Cat. # 638947). The DNA of P ...
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bioRxiv - Microbiology 2023Quote: ... The DNA free RNA was used for cDNA preparation using Takara first strand cDNA synthesis kit (cat no.6110A, Takara Bio Inc., Japan) according to manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: Promoters of genes of interest were cloned from the genomic DNA of MDA-MB-231 cells and cloned into an EcoRI-linearized luciferase reporter vector using In-Fusion cloning (Takara Bio cat. 638948). Lentivirus was packaged using Lipofectamine 3000 following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... 10 ng of RNA was used to prepare cDNA sequencing libraries with the Clontech SMARTer Stranded Total RNA-Seq kit (Pico Input) with ribosomal cDNA depletion (Takara Bio USA, Cat #634413). Prepared libraries were submitted to paired-end 100 bp sequencing on a NovaSeq 6000 system at the YCGA ...
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bioRxiv - Genomics 2019Quote: ... PCR products were collected by centrifugation at ~2250 xg for 20 min using the supplied SMARTer ICELL8 Collection Kit (Takara Bio USA, Cat.#640048).
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bioRxiv - Genomics 2021Quote: ... The 8 source wells of the ICELL8 were loaded with 65 µL of the suspension of tagmented nuclei and dispensed into a SMARTer ICELL8 350v chip (Takara Bio, cat. no. 640019) at 35 nL per well ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The ATP1A1 genes were inserted at the PPH promoter of vectors already containing the corresponding ATP1B1 genes using In-Fusion® HD Cloning Kit (Takara Bio; Cat#638910) and confirmed by sequencing ...
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bioRxiv - Neuroscience 2022Quote: ... Total RNA was reverse transcribed in the presence of PrimeScript™ RT reagent Kit with gDNA Eraser (Perfect Real Time) (Takara, Cat. No. RR047B). For each RT-qPCR reaction ...
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bioRxiv - Neuroscience 2022Quote: ... Total mRNA was reverse transcribed in the presence of PrimeScript™ RT reagent Kit with gDNA Eraser (Perfect Real Time) (Takara, Cat. No. RR047B). Droplet digital PCR was used to measure the levels of transcription factors in isolated MG following a previous method (Fisher et al. ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The relative expression of mRNA and miRNA was respectively quantified using TB Green® PremixExTaq™ II (Tli RNaseH Plus) (Cat# RR820A, Takara, Kyoto, Japan) and miRcute Plus miRNA qPCR Detection Kit (Cat# FP411 ...
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bioRxiv - Cell Biology 2023Quote: ... downstream of the Myh6 (α- myosin heavy chain) promoter and upstream of a human growth hormone polyadenylation signal using In-Fusion HD (Takara Bio cat# 011614). The final transgenic targeting construct was verified by DNA sequencing ...
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bioRxiv - Neuroscience 2023Quote: ... 1.25 x105 cells/cm2 were immediately added to each well with HEK293 maintenance media modified with 10% tetracycline-free FBS (Takara Bio, Cat. No. 631367). After 24 h ...
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bioRxiv - Cell Biology 2024Quote: ... Ten nanograms of RNA were used for preparing indexed libraries using SMARTer Stranded Total RNA-Seq Pico-Input Mammalian kit v.3 (Takara Bio. Cat# SKU: 634487) as per manufacturer’s instructions (except that fragmentation was performed for 3 minutes of fragmentation at 94 °C and 13 cycles was used for PCR2) ...
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bioRxiv - Molecular Biology 2020Quote: ... was amplified by RT-PCR and cloned into pMSCV-IRES-eGFP using In-Fusion® HD Cloning Kit User Manual (Takara, Cat#638920, Shiga, Japan) following the manufacturer’s instructions (see Table 3 for primer sequences).
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bioRxiv - Physiology 2024Quote: Total RNA (1 µg) was used as input for SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian (Cat. # 634875; Takara, Saint-Germain-en-Laye, France). Sequencing was performed on the NextSeq 2000 instrument (Illumina ...
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bioRxiv - Genetics 2024Quote: ... corresponding to predicted CNL and NL protein sequences were synthesized by General Biosystems (Durham, NC, United States) and subsequently used for PCR amplification with PrimeSTAR® Max DNA Polymerase assay (Takara Bio Inc., cat. # R045A) for cloning into pGBKT7 DNA-BD and pGADT7 AD vectors (Table S6) ...
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bioRxiv - Immunology 2022Quote: ... an amount of 1 ng of total RNA was used as input for the SMART-Seq® Stranded Kit (Cat. No. 634444; protocol version “022829”; low input option, Takara Bio USA, San Jose, USA). This kit can deal with degraded as well as high-integrity input RNA ...
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bioRxiv - Cancer Biology 2022Quote: ... The converted cDNA was amplified using gene-specific primers and TB Green® Premix Ex Taq™ II (Cat# RR820A; DSS Takara Bio India Private Ltd) to quantify the expression of the genes using a real-time PCR system (Applied Biosystem Quant Studio 7 Flex ...
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bioRxiv - Immunology 2024Quote: ... The resulting plasmid containing the 3XFlag-ZBP1 was further subcloned in the Bst1 and BamHI sites of the vector pLVX-EF1α-AcGFP1-C1 (Takara, cat# 631984, simplified as pLvx-GFP) by PCR to generate the lentivirus overexpressing vector pLVX-GFP-3XFlag-ZBP1 (simplified as pLvx-GFP-ZBP1OE) ...
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bioRxiv - Neuroscience 2022Quote: ... slices were incubated in a blocking buffer containing the primary antibodies rabbit anti-mCherry (1:1500; cat. No. 632496, Takara Bio USA, Inc., Mountain View, CA, USA) and chicken anti-GFP (1:1500 ...
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bioRxiv - Genomics 2022Quote: ... 0.5 μg of total RNA from each sample was used to synthesize cDNAs from polyA+ RNA using Clontech SMART-Seq® v4 Ultra® Low Input RNA Kit for the Fluidigm® C1™ System (Clontech, Cat. No. 635026) following the manufacturer’s guidelines ...
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bioRxiv - Genomics 2022Quote: ... cDNAs from low yield polyA+ RNA was synthesized using SMART-Seq® v4 Ultra® Low Input RNA Kit for the Fluidigm® C1™ System (Clontech, Cat. No. 635026), SMARTer-seq® chemistry (Switching Mechanism at 5’ End of RNA Template and Locked Nucleic acid technology) ...