Labshake search
Citations for Takara Bio :
1201 - 1250 of 1348 citations for pVectOZ CAT Transfection control since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... Additional to the NF-κB binding motifs NF-κB response element and a TATA-like sequence from pNFκB-Luc vector (Clontech Cat. No. 631904) were added to the Firefly luciferase gene and introduced into pCDH-CMV-EF1-Puro.
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bioRxiv - Microbiology 2020Quote: ... Ad5 stocks were tittered by detection of the Ad hexon protein using Adeno-X™ Rapid Titer Kit (Cat. No. 632250, Takara, Clontech). The Ad5 CPE was dose-dependent ...
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bioRxiv - Neuroscience 2021Quote: The following antibodies were used for immunohistochemistry with dilution ratios as indicated: rabbit anti-DsRed (1:1,000, Clontech cat# 632496, RRID: AB_10013483), mouse anti-BRP (1:100 ...
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bioRxiv - Microbiology 2022Quote: ... HEK293 cells were cotransfected with the S expression plasmids (400 ng) and pDSP8-11 (400 ng) using TransIT-LT1 (Takara, Cat# MIR2300). To prepare target cells ...
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bioRxiv - Neuroscience 2022Quote: Between 1-3 ng of the nuclear RNA was used in the SMART-seq v4 Ultra Low Input RNA Kit for Sequencing (Takara, cat # 634888) according to the manufacturer’s protocols ...
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bioRxiv - Microbiology 2022Quote: ... An internal ribosomal entry site (IRES) was inserted behind the ACE2 sequence via restriction digestion of a pEF1a-IRES vector (Cat. n° 631970, Takara Bio Inc.) with NheI-HF and SalI-HF (NEB) ...
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bioRxiv - Developmental Biology 2021Quote: Doxycycline-inducible overexpression of transcription factors was achieved using the Lenti-X Tet-On 3G Inducible Expression System (Clontech; Cat. No. 631363) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... Pooled RNA from two previously identified positive NP swab samples was used for first strand cDNA synthesis (Takara primescript kit cat # 6110A). 20 μl of the cDNA reaction was diluted ten-fold and used for primary PCR ...
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bioRxiv - Molecular Biology 2021Quote: ... The chip was then loaded again on Fluidigm C1 and cDNA was synthesised and pre- amplified in the chip using Clontech SMARTer kit (Takara Clontech, Cat# 634833). In batch B ...
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bioRxiv - Microbiology 2020Quote: ... Cell supernatants were harvest 24 hours post-infection and RNA was extracted by a viral RNA extraction kit (Takara, Cat no. 9766). The number of viral copies was detected by real-time quantitative reverse transcription PCR (qRT-PCR ...
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bioRxiv - Microbiology 2021Quote: ... HEK293 cells were cotransfected with the expression plasmids for D614G S or D614G/P681R (400 ng) with pDSP1-7 (400 ng) using TransIT-LT1 (Takara, Cat# MIR2300). To prepare target cells ...
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bioRxiv - Cancer Biology 2020Quote: ... the OriGene vector SC303275 containing the cDNA of SOX11 was amplified by PCR and the obtained fragment was gel purified and ligated into the opened NdeI site of response vector pLVX-TRE2G-Zsgreen1 (Takara, cat#631353) producing pLVX-TRE3G-Zsgreen1-IRES-hSOX11 ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were later processed for single cell RNA-seq using SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Takara Cat# 634894) as previously described41,45.
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bioRxiv - Neuroscience 2022Quote: ... double-stranded cDNA whole transcriptome library was synthesized from 1µg of total RNA with Takara Bio’s SMARTer Stranded Total RNA Sample Prep Kit – HI Mammalian (Takara Bio, Cat. No. 634876) and SMARTer RNA Unique Dual Index Kit (Takara Bio ...
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bioRxiv - Evolutionary Biology 2019Quote: ... This vector was generated by splicing PCR products of the MpALSm gene with the 35S promoter together with the MpEF1α promoter into a XhoI/PstI-digeststed pCAMBIA backbone using the Clontech In-Fusion kit (Clontech Cat. #: 638909) and the primers indicated in Table 1 ...
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bioRxiv - Genetics 2019Quote: ... We amplified the fragment by PCR (see Table S3 for the primer sequences) with Tks Gflex™ DNA Polymerase (Takara, Cat#R060A) and purified it ...
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bioRxiv - Molecular Biology 2019Quote: ... The libraries for RNA sequencing were generated using the SMART-Seq v.4 Ultra Low Input RNA kit (Takara Bio, cat. #634890) and Nextera XT DNA Library Prep Kit (Illumina ...
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bioRxiv - Microbiology 2019Quote: ... Low cell RNA-seq libraries (unstranded) wer generated withSMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Takara/ Clonetech; Cat#634890). All sequencing libraries were sequenced on a HiSeq 2500 system (Illumina ...
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bioRxiv - Neuroscience 2019Quote: Sections were immunolabeled using an anti-DsRed antibody that detects the protein product generated by the recombined R26tdTomato reporter allele (anti-dsRed Ab from Clontech, Catalog (Cat) #632496 ...
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bioRxiv - Genomics 2019Quote: ... The Agilent 2100 Bioanalyzer was used to assess RNA quality and only high-quality RNA (RIN > 8) was further processed for cDNA synthesis with SMART-Seq v4 Ultra Low Input RNA Kit (Clontech cat. 634888) according to the manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2020Quote: Libraries from RPFs isolated from HEK293T cells were prepared using the SMARTer® smRNA-Seq Kit for Illumina (Takara, Cat. No. 635029) and sequenced with 50 cycles single-read on an Illumina NovaSeq 6000 sequencer ...
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bioRxiv - Microbiology 2019Quote: ... A pair of primers that match the sr8384 sequence were separately added into the total RNA to synthesize the first-strand cDNA by using the PrimeScript RT Reagent Kit (TaKaRa, cat. #RR047A). Next ...
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bioRxiv - Physiology 2019Quote: cDNA library was prepared using SMART-Seq v4 3’ DE kit according to the manufacturer’s instructions (Clontech Laboratories Inc. Cat. No. 635040). For each clone ...
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bioRxiv - Genomics 2021Quote: ... Purified RNA samples were diluted to 1 μg/μL and pooled to perform cDNA synthesis by utilizing PrimerScript first strand cDNA synthesis kit (Cat. no. 6110A, Takara Bio, Japan), following the manufacturer’s protocol ...
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bioRxiv - Genomics 2021Quote: ... the volume of 10 mM TAPS Buffer pH 8.5 was adjusted to 65 µL per 20,000 nuclei to yield a concentration of ~300 nuclei/µL and nuclei were stained with 1X DAPI and 1X secondary diluent reagent (Takara Cat# 640196). The 8 source wells of the ICELL8 were loaded with 65 µL of the suspension of tagmented nuclei and dispensed into a SMARTer ICELL8 350v chip (Takara Bio ...
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bioRxiv - Immunology 2020Quote: ... Cells were counted and seeded at 3 million cells in 1 mL of media with 2x hIL-2 into each well of a 6 well plate that was coated with 15 µg/mL of RetroNectin (Takara, Cat# T100A) for 3 hours at room temperature and subsequently washed with 1x PBS ...
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bioRxiv - Genomics 2021Quote: Paired-end RNA-seq libraries were constructed from >747 pg of RNA using the SMART-Seq v4 ULTRA Low Input RNA Kit for Sequencing (Takara, cat. 634891) and NexteraXT kits (Illumina ...
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bioRxiv - Developmental Biology 2022Quote: 1 µg of tissue specific (ovary) RNA was used for First strand cDNA synthesis using PrimeScriptTM 1st strand cDNA Synthesis Kit (Takara, Cat# 6110A) using the following program ...
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bioRxiv - Biochemistry 2022Quote: ... S domains and variants were purified similar to S protein using nickel resin (10 mL/L, His60 Ni2+ superflow resin, Takara cat# 635664) with wash buffer (25 mM MES ...
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bioRxiv - Biochemistry 2022Quote: ... the RNAs of 150µl of the supernatant were extracted and quantified using Retro-X™ qRT-PCR Titration Kit (Takara, cat# 631453), according to the manufacturer instruction.
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bioRxiv - Immunology 2022Quote: ... 10 ng RNA were used and 7 PCR cycles were carried out to synthesise cDNA with the SMART-Seq® v4 Ultra® Low Input RNA Kit (cat # 634891, Takara). RNAseq libraries were prepared with the Nextera XT DNA Library Preparation Kit (cat# FC-131–1096 ...
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bioRxiv - Microbiology 2022Quote: ... HEK293 cells were cotransfected with the S expression plasmids (400 ng) and pDSP8-11 (ref.65) (400 ng) using TransIT-LT1 (Takara, Cat# MIR2300). On day 3 (24 hours posttransfection) ...
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bioRxiv - Neuroscience 2023Quote: pLV-hsynapsin-SRGAP2C-HA-WPRE was generated by insertion of SRGAP2C-HA at a multiple cloning site (MCS) of a pLV-hsynapsin-MCS-WPRE plasmid using InFusion cloning (Clontech, Cat#638909). The MCS was prepared by annealing the following primers pair ...
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bioRxiv - Molecular Biology 2022Quote: ... 2 µg of high-quality total RNA (RNA integrity number > 8) was fragmented in the presence of Mg2+ in SMARTScribe Reverse Transcriptase buffer (Clontech, Cat. 639537), and mRNA fragments with a poly(A ...
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bioRxiv - Plant Biology 2023Quote: ... The resulting fragments were cloned into vector backbones derived from pPY22 (mNeonGreen-Hygromycin B) or pPY23 (mNeonGreen-Nourseothricin) using the In-Fusion Snap Assembly Kit (Takara, cat. 638948). For PpREN knockout ...
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bioRxiv - Neuroscience 2024Quote: ... and individual cells were captured in separate wells of a 96-well plate containing 4 μl lysis buffer (1 U/μl RNase inhibitor [Clontech, Cat#: 2313B]), 0.1% Triton (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: cDNA was prepared from 48-hour cultured cells using CellAmp Direct Lysis and RT set (Takara Bio, Shiga, Japan, Cat# 3737S/A) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... Lentivirus-containing conditioned media were centrifuged at 500 x g for 5 min at 4°C to remove cellular debris and concentrated using Takara LentiX (Takara Cat# 631231). Briefly ...
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bioRxiv - Plant Biology 2024Quote: ... 1000 ng of DNase-treated RNA was used in a 20 µL reaction employing oligo-dT primers and random hexamers according to the protocol provided by Takara (Cat# RR014B). The relative expression levels of the target genes were assessed via qPCR ...
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bioRxiv - Immunology 2023Quote: ... and used to generate Illumina-ready heavy and light chain sequencing libraries using the SMARTer Mouse BCR IgG H/K/L Profiling Kit (Takara, Cat# 634422). Briefly ...
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bioRxiv - Plant Biology 2024Quote: ... targeting hSpCas9 to NRPE1 (AT2G40030) exons were sequentially cloned in the SpeI linearized pYAO::hSpCas9 construct by In-Fusion (Takara, cat #639650). The final vector was electroporated into AGL0 agrobacteria and transformed in h1 mutant plants by agrobacterium-mediated floral dipping ...
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bioRxiv - Microbiology 2023Quote: ... HEK293 cells were cotransfected with the S expression plasmids (400 ng) and pDSP8-11 (ref.59) (400 ng) using TransIT-LT1 (Takara, Cat# MIR2300). On day 3 (24 h posttransfection) ...
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bioRxiv - Immunology 2023Quote: Full length cDNAs were generated and amplified directly from a single cell through the SMART-Seq HT Kit (Takara Cat. No. 634437). Up to 200pg cDNAs were used to generate the dual index Illumina libraries using Nextera XT DNA Library Prep Kit (Illumina Cat No ...
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bioRxiv - Microbiology 2022Quote: ... HEK293 cells were cotransfected with the S expression plasmids (400 ng) and pDSP8-11 (ref.58) (400 ng) using TransIT-LT1 (Takara, Cat# MIR2300). On day 3 (24 hours posttransfection) ...
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bioRxiv - Cancer Biology 2023Quote: The MDM4 coding sequence (NM_002393.5) was cloned into Lenti-X™ Tet-One™ Inducible Expression System (Takara Bio, cat. no. 631847). The resulting plasmid was then subject to whole-plasmid sequencing for verification ...
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bioRxiv - Microbiology 2023Quote: ... nine DNA fragments encoding the partial genome of SARS-CoV-2 (hCoV-19/Japan/TY-WK-521/2020) were amplified by PCR using PrimeSTAR GXL DNA polymerase (Takara, Cat# R050A). A linker fragment encoding the hepatitis delta virus ribozyme ...
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bioRxiv - Neuroscience 2023Quote: ... with primers 5’-TCTTCCACTAGTGCCACCATGGCCACAGCTTGTAAAAGATC-3’ and 5’-TCTTCCGGATCCTTACAGCATGCCAAATCCTTTGG-3’ and subcloning into the SpeI and BamHI sites of the pLVX-IRES-mCherry vector (Clontech Cat#631237). HEK293T cells were transfected in 96-well using Lipofectamine 3000 Reagent (ThermoFisher ...
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bioRxiv - Immunology 2023Quote: ... cDNA was then used for qPCR analysis using the TB Green® Premix Ex Taq™ II (Ti RNase H Plus) (Cat. No. RR820A, Takara) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: The pik1-11 temperature sensitive allele was constructed as described (Tang et al., 2019) with the exception that EX taq polymerase (Takara, cat# 4025) and accompanying dNTPs (Takara ...
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bioRxiv - Evolutionary Biology 2023Quote: The 3’UTR sequence of CcTRPM gene was amplified by the 3’-Full RACE Core Set with PrimeScriptTM RTase kit (Cat# 6106, Takara, Kyoto, Japan). miRNAs for CcTRPM were predicted by a service provider LC science with two software programs of miRanda (http://www.microrna.org ...