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Citations for Takara Bio :
901 - 950 of 1168 citations for Mouse ATPAF2 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2021Quote: ... To verify deletions or test for the presence of the CPOT plasmids colony PCR was performed using SapphireAmp fast PCR mix (TaKaRa Bio). For DNA construction ...
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bioRxiv - Cell Biology 2021Quote: The fragments containing the promoter regions of YPK1 and LCB1 were amplified by means of PCR and cloned into the XhoI and BamHI sites of the 4×CDRE-LacZ fusion plasmid, pAMS366 (38) (gifted by Dr. Martha Cyert, Stanford University) using the In-Fusion® Cloning Kit (Clontech), resulting in PYPK1-LacZ and PLCB1-LacZ reporter plasmids ...
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bioRxiv - Biochemistry 2020Quote: Induced expression of the bidirectional plasmid required the use of HEK 293 Tet-On® 3G cells (Takara Clontech Cat# 631182). Cells in 6 well plates were transfected with 1 μg of the bidirectional plasmid using lipofectamine 3000 (Thermo Fisher Scientific Cat# L3000-008 ...
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bioRxiv - Biochemistry 2020Quote: Induced expression of the bidirectional plasmid required the use of HEK 293 Tet-On® 3G cells (Takara Clontech Cat# 631182). Cells in 6 well plates were transfected with 1 μg of the bidirectional plasmid using lipofectamine 3000 (Thermo Fisher Scientific Cat# L3000-008 ...
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bioRxiv - Plant Biology 2020Quote: ... We generated a plasmid encoding Pikh-HMA in pGBKT7 and co-transformed it into chemically competent Y2HGold cells (Takara Bio, USA) with the individual AVR-Pik variants in pGADT7 as described previously [20 ...
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bioRxiv - Plant Biology 2020Quote: ... For Yeast-Two-Hybrid assays HvRIPb and HvRIPb truncations were subcloned from the pGEM-T easy vector into pGADT7 and pGBKT7 plasmids (Clontech Laboratories) using the EcoRI and BamHI restriction sites ...
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bioRxiv - Cell Biology 2021Quote: ... The coding sequences of Arfs were amplified by PCR (primers listed in Suppl. Table S1) and inserted into the pQXCIP plasmid (Takara Bio) using the AgeI and BamHI restriction sites ...
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bioRxiv - Cell Biology 2021Quote: ... and cloned into a lentiviral targeting plasmid pLVX-EF1a-mCherry-N1 without the mCherry portion (631986, Takara Bio, Mountain View, CA) by Gibson assembly ...
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bioRxiv - Immunology 2022Quote: ... The g block and linearized plasmid backbone were ligated together using the In-fusion HD cloning kit (Takara Bio, catalog #638920).
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bioRxiv - Microbiology 2022Quote: ... The PCR product was purified and ligated into the pET15b vector with a C-terminal His tag to create plasmid pET-AdpA by using the ClonExpress™ II One Step Cloning Kit (TaKaRa). The plasmid was transformed into E ...
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bioRxiv - Plant Biology 2022Quote: The lethal gene ccdB flanked by BsmBI sites was inserted into the positions 245 and 246 of Eggplant latent viroid (ELVd) accesion AJ536613.1 and cloned into a pBluescript SK plasmid using high-fidelity PrimeSTAR HS DNA Polymerase (Takara, Kusatsu, Japan). This plasmid linearized with BsmBI was used to ligate self-hybridized oligonucleotides into the positions 245 and 246 of ELVd ...
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bioRxiv - Biochemistry 2022Quote: ... HEK-293T cells were transfected with 1 µg of TRPC6 channel plasmids (WT, T221A and 112Q) using Xfect transfection reagent according to manufacturer’s protocol (Takara Bio 631318). Cells expressing wild type and mutant channels were identified by YFP fluorescence ...
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bioRxiv - Bioengineering 2022Quote: ... Purified insert DNA was cloned into the linearized modRNA plasmid (5MCS3)(K et al., 2018) using the In-Fusion Cloning Kit (Takara Bio). The DNA template for modRNA synthesis was PCR amplified from the successfully cloned 5MCS3 plasmid followed by PCR purification using DNA Clean & Concentrator-5 (Zymo Research) ...
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bioRxiv - Microbiology 2021Quote: ... K13 donor sequences were amplified from the pGEM vector using the primer pair p21+p22 and sub-cloned into the pDC2-cam-coSpCas9-U6-gRNA-hdhfr plasmid at the EcoRI and AatII restriction sites by In-Fusion® Cloning (Takara). The final plasmids were then sequenced using primers p23 to p25 (Table S9) ...
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bioRxiv - Molecular Biology 2020Quote: ... we first produced lentivirus with Pup (E) plasmid Bio-Pup (E)-IRES-BFP within the Tet-On 3G inducible expression system (Clontech 631168), and infected OVCAR-5 cells for 48 hours ...
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bioRxiv - Cell Biology 2020Quote: ... putative miR-31-5p binding sequences were deleted in the plasmid DNA using PrimeSTAR Mutagenesis Basal Kit (TaKaRa Bio, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... The amplified cDNAs were cloned into the indicated plasmids using an In-Fusion HD cloning kit (Clontech, Mountain View, CA, USA). The sequences of all plasmids were confirmed by Eurofins Genomics (Tokyo ...
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bioRxiv - Microbiology 2022Quote: ... the coding sequences of DivIVA and its T19A and T19E mutant alleles were PCR amplified using sequence-specific primers (Table 1) and cloned at BamHI-KpnI sites in pDsRed plasmid (Clontech Inc.) yielding pDsD4A and pDsD4AT19A ...
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bioRxiv - Microbiology 2022Quote: ... expressing Moloney murine leukemia virus gag and pol genes was co-transfected with pLNCX2 vector with the FLAG-APEX2-GARG1060 insert and a plasmid coding for the vesicular stomatitis virus envelope glycoprotein (Takara Bio) using Mirus 2020 DNA transfection reagent (Mirus) ...
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bioRxiv - Systems Biology 2022Quote: ... Plasmids containing mKO2-hCdt1 (30-120) and mAG-hGeminin (1-110) were co-transfected with envelope and packaging plasmids into LentiX-293T cells (Takara bio) to generate lentiviral particles ...
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bioRxiv - Genomics 2022Quote: ... 1.51 million bacterial colonies were collected and the plasmids were extracted with NucleoBond Xtra Maxi EF kit (Takara Bio USA #740424). To insert a TmU DNA fragment containing the tracRNA (E/F ...
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bioRxiv - Immunology 2022Quote: ... the variant HXP-S were inserted into the pNDV_LS/L289A rescue plasmid (between P and M genes) by in-Fusion cloning (Clontech, CA, USA). The recombinant product was transformed into MAX Efficiency™ Stbl2™ Competent Cells (Thermo Fisher Scientific ...
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bioRxiv - Synthetic Biology 2022Quote: ... Cas9GCN4 and the catalytic core of chromatinmodifying enzymes were cloned into PiggyBac recipient plasmids by homology arm recombination using In-fusion HD cloning (Takara #639650). Specifically ...
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Biosynthesis of Circular RNA ciRS-7/CDR1as Is Mediated by Mammalian-Wide Interspersed Repeats (MIRs)bioRxiv - Molecular Biology 2019Quote: ... The expression plasmids for circSPNS1 was constructed by subcloning the PCR-amplified fragments into FLAG-pcDNA3 plasmid using an In-Fusion HD cloning kit (Takara Bio) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2019Quote: ... murine Ptpn2 cDNA from HER-2-E0771 cells was reverse transcribed and amplified by PCR and cloned into the SmaI and EcoR1 restriction sites of Tre-3G plasmid (Clontech Laboratories) to generate the Tre-3G-Ptpn2 construct ...
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bioRxiv - Molecular Biology 2019Quote: ... and cloned into the BtgZ1 restriction site of the pL6-egfp plasmid as described elsewhere61 using the In-Fusion HD cloning kit (Clontech # 639649). For the gControl gRNA ...
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bioRxiv - Neuroscience 2019Quote: ... HeLa cells were transfected with 3 μg of the designated construct or empty plasmid (see below) using the CalPhos transfection kit (Clontech, 631312). HeLa cells were used 48 h after transfection.
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bioRxiv - Plant Biology 2021Quote: ... the open reading frame of the OBE3 gene was cloned into XmaI/BamHI sites of the pGADT7 plasmid (Clontech, Palo Alto) resulting into pEW11.
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bioRxiv - Molecular Biology 2021Quote: ... a 659-bp DNA fragment of DsRed2 was amplified from 1 ng of pDsRed2-N1 plasmid (Clontech, Mountain View, CA, USA) with primers (5′-TAATACGACTCACTATAGGGCGTGCACTCGTACACTGAGG-3′ and 5′-TAATACGACTCACTATAGGGTCATCACCGAGTTCATGCG-3′) ...
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bioRxiv - Immunology 2021Quote: ... The retroviral transfer vector was co-transfected with pVSV-G retroviral envelope-expressing plasmid into the GP2-293 cell line (Clontech-TaKaRa) using Lipofectamine 2000 reagent (Invitrogen ...
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bioRxiv - Physiology 2021Quote: ... K13 donor sequences were amplified from the pGEM vector using the primer pair p21+p22 and sub-cloned into the pDC2-cam-coSpCas9-U6-gRNA-hdhfr plasmid at the EcoRI and AatII restriction sites by In-Fusion® Cloning (Takara). The final plasmids were then sequenced using primers p23 to p25 ...
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bioRxiv - Microbiology 2020Quote: ... The PCR products were purified and fused into a ClaI-digested plasmid with an In-Fusion HD cloning kit (TAKARA, Japan). Mutants for overexpressing HFB2 (TgOPB38530 ...
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bioRxiv - Cell Biology 2021Quote: ... we first produced lentivirus with Pup(E) plasmid Biotin-Pup(E)-IRES-BFP within the Tet-On 3G inducible expression system (Clontech 631168), and infected Jurkat cells for 48 hours ...
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bioRxiv - Biochemistry 2021Quote: ... falciparum cDNA followed by Ligation Independent Cloning into HindIII/KpnI-cleaved plasmid pOPIN F (82) using the In-Fusion HD EcoDry Cloning Kit (Takara Clontech) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... His-Raf1, His-ATG8) and empty plasmids (GST tag, 6×His tag) were transformed into component cells Escherichia coli BL21 (Takara, 9126) or BL21 (DE3 ...
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bioRxiv - Microbiology 2020Quote: ... The cohesive ends of the linearized plasmid were blunt-ended and self-ligated using a DNA Blunting Kit (Code No. 6025, Takara Bio). The DNA Blunting Kit can smooth DNA with 3ʹ- or 5ʹ- protruding ends ...
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bioRxiv - Microbiology 2020Quote: ... The obtained linearized plasmid was excised from the agarose gel and purified using a MiniBEST Agarose Gel DNA Extraction Kit (Takara Bio). The cohesive ends of the linearized plasmid were blunt-ended and self-ligated using a DNA Blunting Kit (Code No ...
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bioRxiv - Molecular Biology 2020Quote: Yeast-2-hybrid assays were performed with the plasmids and strains from the Matchmaker Gold Yeast Two-Hybrid System (Takara Bio) according to manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were cotransfected with hSyn-DsRed and the indicated construct on DIV 7 or DIV 19 (for older neurons) with 1 μg total purified plasmid DNA via CalPhos Mammalian Transfection Kit (Takara Bio) according to the manufacturer’s protocol.
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bioRxiv - Molecular Biology 2022Quote: ... serving as homology arms for directional cloning of the library into RD-seq plasmid (zfh1-DSCP-Gal4-DBD, Suppl. Table 2) vector using In-Fusion HD (Clontech 639650).
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bioRxiv - Cell Biology 2022Quote: ... packaging (pCAG-HIVgp) and envelope (pCMV-VSV-G-RSV-Rev) plasmids were co-transfected into Lenti-X 293T cells (Takara Bio) using Fugene 6 reagent (Promega) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: The pSLI-HA-FKBP-T2A-yDHOH-Ribozyme plasmid was generated in our laboratory from the pSLI plasmid32 by In-fusion® (Takara) insertion of five PCR fragments coding for ...
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bioRxiv - Plant Biology 2022Quote: ... Plasmid vectors were linearized to generate templates for viral RNA in vitro transcription (T7 RNA Polymerase System, Takara Bio, Shiga, Japan). RNA1 to RNA3 were equally mixed to infect 3-week-old N ...
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bioRxiv - Neuroscience 2022Quote: ... Barnase and barstar from Bacillus amyloliquifasciencs cDNAs were codon-optimized and synthesized with homologous ends to existing Nlgn1 or Nrxn3β plasmids and ligated with In-fusion enzyme (Takara Bio). A graphical summary of the design of major fusion constructs used can be found in Fig S2 ...
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bioRxiv - Plant Biology 2022Quote: ... in pGADT7 were combined with each other and with empty control plasmids using the Matchmaker™ Gold Yeast Two-Hybrid System (Clontech). The vectors were co-transformed into the Y2Hgold MATa Yeast strain ...
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bioRxiv - Plant Biology 2023Quote: Plasmids for targeted gene deletion by homologous recombination were assembled using the In-Fusion® HD Cloning Kit (Takara Bio, Japan). Briefly ...
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bioRxiv - Microbiology 2023Quote: 0.35×105 293TT cells per plate were plated in 24-well plates 16-20 h prior to transfection of plasmids encoding HA-tagged CA Rab9a or the empty vector as control (Takara, 632105). 48 h after transfection ...
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bioRxiv - Microbiology 2023Quote: ... the cells were transfected with the eukaryotic expression plasmid harbouring USP8 (pCMV-HA-USP) or Empty vector (pCMV-HA) using X-fect (TAKARA Bio) transfection reagent according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2023Quote: ... The generated PCR products for the full-length proteins were transferred into the pMET7-GAG-sp1-RAS plasmid by classic cloning with restriction enzymes (EcoRI and XbaI) or In-Fusion seamless cloning (Takara Bio) when the genes contained internal restriction sites for EcoRI and XbaI (which was the case for CSDE1 ...
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bioRxiv - Biochemistry 2022Quote: ... KM881710.2) were PCR amplified from viral cDNA templates (31–33) and cloned into pSUMO expression plasmid by IN-FUSION (Takara Bio). The final constructs were confirmed by sanger sequencing performed by Genewiz.