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Citations for Takara Bio :
851 - 900 of 1168 citations for Mouse ATPAF2 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... A repair plasmid to replace the coding sequence of SOS1 with an HXGPRT-mCherry construct was generated by amplifying the 5’ and 3’ UTRs of SOS1 (adjacent to the protospacers in the Cas9-sgRNA plasmid) using primers 81-84 and inserting the resulting fragments into the pTKO2 plasmid27 by In-Fusion cloning (Takara). Parasites were co-transfected with both the Cas9-sgRNA and pTKO2 plasmids ...
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bioRxiv - Immunology 2023Quote: ... Lentiviral particles were produced by co-transfecting the barcoding plasmid with the packaging and envelope plasmids pMD2G and pCMV-dR8.91 (provided by Andrew Ramos) into Lenti-X 293T cells (Takara, 632180) with Lipofectamine 2000 (Thermo Fisher ...
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bioRxiv - Plant Biology 2023Quote: ... pGADT7 plasmids encoding the effector domain of different ZiF were co-transformed into chemically competent Y2HGold cells (Takara Bio, USA) with a pGBKT7 plasmid encoding OsExo70F3 in using a Frozen-EZ Yeast Transformation Kit (Zymo research).
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bioRxiv - Molecular Biology 2023Quote: ... The 5’UTR synthetic intron fragment was synthesized (Twist Biosciences) and cloned into the AgeI restriction site in all reporter plasmids via InFusion (Takara). These plasmids are available through Addgene (Addgene ID#s available upon publication).
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bioRxiv - Cell Biology 2023Quote: ... 91 The plasmids pME-rtTA and p3E-TRE were made by inserting the Tet-On fragments from the Tet-On system (Clontech) in the appropriate Tol2 entry vectors ...
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bioRxiv - Cell Biology 2023Quote: ... Expression plasmids encoding mCherry-tagged PDZ-RhoGEF and its mutants were constructed by inserting the PCR-amplified cDNA fragments into an mCherry-C1 expression plasmid (Clontech). The cDNA fragments were amplified using PrimeSTAR HS DNA polymerase (TAKARA ...
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bioRxiv - Cell Biology 2023Quote: Sequence confirmed cDNAs encoding Snail and OVOL2 were obtained from the DNASU plasmid repository (Arizona State University) and subcloned into pLVX-TetOne-Puro (Takara) vector ...
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bioRxiv - Developmental Biology 2023Quote: The dCas9-KRAB-NLS-NLS sequence was amplified from the plasmid PB-TRE-dCas9-KRAB-MeCP2 (Tan, 2022) (addgene 122267) using PrimeSTAR Max 2 ×premix (Takara). A T7 promoter sequence was included in forward primer for in vitro transcription using the mMESSAGE mMACHINE T7 Ultra Transcription kit (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2023Quote: ... LAT1 mutants with amino acid substitutions or an N-terminal truncation (Δ1-50) were constructed by whole-plasmid PCR using PrimeSTAR MAX DNA polymerase (Takara). The corresponding codons were altered as follows for amino acid substitution ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were transfected with GRIK1-1a or GRIK1-2a cloned in pRK7 (in the presence or absence of rNeto1/rNeto2 cloned in IE-pRK8 as indicated) with EGFP expressing plasmid using Xfect (Clontech) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... A homology-directed repair template donor vector where frt-PGK-Hygro-pA-frt-T2A-mScarlet-Akaluc-T2A-CreER-P2A-DTR is flanked by the homology arms was cloned into the pUC19 plasmid backbone (#638949, Takara). Donor vector and RNP complex were transfected into mESC line harboring Krasfrt-stop-frt(LSL)-G12D/+ ...
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bioRxiv - Cell Biology 2024Quote: ... where the entire ORF of the corresponding gene was inserted in frame into the pGADT7 (GAL4 activation domain) or pGBKT7 (GAL4 binding domain) plasmid (Clontech).
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bioRxiv - Evolutionary Biology 2024Quote: ... Reporter plasmid libraries were made by cloning amplified ATAC fragments into AgeI-HF- and SalI-HF-linearized pSTARR-seq plasmid using InFusion HD cloning kit (Takara) and then propagated in MegaX DH10B T1R electrocompetent bacteria ...
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bioRxiv - Plant Biology 2023Quote: ... Direct protein-protein interaction was confirmed by co-transformation of the respective plasmids into the yeast strain AH109 using the Matchmaker GAL4 Two-hybrid System (Clontech), followed by a selection of transformants on 2% SD (-Leu/-Trp ...
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bioRxiv - Cancer Biology 2023Quote: ... and clonally isolated p53 KO cell lines were electroporated with 3 µg p53 firefly luciferase reporter plasmid pp53-TA-Luc (Clontech/Takara) and 0.3 ug renilla luciferase control plasmid pRL-CMV (Promega ...
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bioRxiv - Synthetic Biology 2024Quote: ... The Gal4-NGN2 plasmid was constructed using Gibson Assembly with In-Fusion® Snap Assembly Master Mix (Takara Bio #638947) and following recommended manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: Lentivirus was generated by co-transfecting the barcoding plasmid with the packaging and envelope plasmids pMD2G and pCMV-dR8.91 (provided by Andrew Ramos) into Lenti-X 293T cells (Takara, 632180) using Lipofectamine 2000 (Thermo Fisher ...
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bioRxiv - Cell Biology 2023Quote: HeLa cells and hippocampal neurons were transfected with plasmids encoding FM4 recombinant receptors for 15 h and then treated with 2 μM DD-Solubilizer (TakaraBio/Clontech) to induce the release of the receptors from the ER into the secretory trafficking ...
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bioRxiv - Molecular Biology 2024Quote: The RBD-DsRed plasmid was created by PCR cloning the HB domain of RNAse H1 into the pDsRed-Express-C1 vector (Clontech), following the previously described method [32].
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bioRxiv - Microbiology 2024Quote: Each individual SARS-CoV-2 fragment was amplified from their respective plasmids using an exclusive pair of primers (Supplementary Table 2) and high-fidelity PrimeSTAR GXL DNA polymerase (Takara), followed by gel isolation with NucleoSpin Gel and PCR Clean-up (Macherey-Nagel ...
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bioRxiv - Microbiology 2024Quote: ... The donor vectors were cloned either to the pGADT7-GW prey or the pGBKT7-GW bait plasmids (Takara, Kusatsu, Japan) and simultaneously transformed to the Saccharomyces cerevisiae AH109 strain (Clontech ...
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bioRxiv - Microbiology 2024Quote: ... was cloned from a synthetic gene block and inserted into XbaI and EcoRI linearised empty plasmid pEXT21 (pHD0788) via restriction less cloning (InFusion, Takara). A signal-peptide was included 5’ of the IdeS fragment ...
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bioRxiv - Molecular Biology 2024Quote: ... a double-stranded DNA fragment corresponding to the loxJT15+C290C+17:m7+FLAG+17:HT1+HA+17:HT2+MYC+17:N+V5 portion was synthesized (Integrated DNA Technologies) and inserted at the C-terminus of GFP within the pEGFP-C3 plasmid (Clontech) using a GenBuilder Cloning kit (GenScript) ...
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bioRxiv - Molecular Biology 2024Quote: ... The N-terminal His tag construct of human MPST was co-transformed with GroES-EL chaperon plasmid from Takara (#3340), overexpressed in E ...
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bioRxiv - Microbiology 2023Quote: ... the pLVX-G9R+A1L-puromycin or pLVX-G9R+A1L-blasticidin plasmids were transfected into 293T cells using the Lenti-X Packaging Single Shots kit (631275, TaKaRa). Lentiviral supernatants were harvested 48 hours post-transfection and filtered through a 0.45 μm membrane (SLHV033RB ...
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bioRxiv - Microbiology 2024Quote: ... individual FLAG-HA tagged Vpr genes were amplified from the pLRGatewayIRESeYFP-Vpr plasmids using the primers in Table 2 and subcloned into pLVX-TetOne-puro (Clontech) using EcoRI and BamHI restriction sites ...
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bioRxiv - Cell Biology 2024Quote: ... The PCR amplified metr-1(cDNA) sequence was then fused with the linearized plasmid carrying the Prgef-1 promoter through InFusion cloning (Takara) methodology ...
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bioRxiv - Genomics 2023Quote: ... Digests were run on a 0.8% agarose-TAE gel and full-length plasmids were extracted with the Nucleospin PCR and Gel Cleanup Kit (Takara, 740609). A LR reaction was performed with 150 ng entry library and 150 ng pDEST_HC_Rec_Bxb_v2 ...
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bioRxiv - Bioengineering 2024Quote: ... Appropriate colonies were grown and the recombinant bacmid DNA was extracted using the NucleoBond Xtra Midi plasmid purification kit (TAKARA). The fifth instar silkworm larvae ...
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bioRxiv - Cell Biology 2024Quote: ... Pairs of pGBKT7 and pGADT7 plasmid were co-transformed into the yeast strain AH109 following the MatchmakerTM GAL4 Two-Hybrid System instructions (Clontech). Primary transformants were selected on synthetic drop-out (SD ...
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bioRxiv - Physiology 2024Quote: ... the full-length HA-TTL was amplified from the HA-TTL-IRES-dsRed plasmid using PrimeStar GLX Polymerase (Takara Clontech) and ligated into the NheI and BamHI restriction sites of VC206 ...
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bioRxiv - Physiology 2024Quote: ... the full-length HA-TTL was amplified from the HA-TTL-IRES-dsRed plasmid using PrimeStar GLX Polymerase (Takara Clontech) and ligated into the NheI and BamHI restriction sites of VC206 ...
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bioRxiv - Cell Biology 2019Quote: ... Antibodies used for this study were: mouse anti-GFP JL-8 (Clontech, 1:3000), rabbit anti-PfEF1α (from D ...
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bioRxiv - Immunology 2020Quote: Mouse tail DNA was amplified using KAPA Taq PCR Kit (Takara Bio, cat. R040A) and primers Rosa26 WT F ...
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bioRxiv - Genetics 2019Quote: ... Enzyme immunoassays were used to measure plasma concentrations of mouse Glu-Osteocalcin (MK129;Takara), mouse Gla-Osteocalcin (MK127 ...
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bioRxiv - Neuroscience 2021Quote: Total RNA from mouse testis and the whole brain was either obtained from Takara or prepared from 100 mg of testis tissue from 3-month old WT and βV KO C57BL/6J animals with Trizol reagent (Chomczynski & Mackey ...
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bioRxiv - Cell Biology 2021Quote: ... We probed the blot with Mouse anti-GFP (JL8, Living Colors, Takara, 1:3000) or Mouse anti Ds-Red (Santa Cruz Biotechnology ...
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bioRxiv - Cell Biology 2022Quote: ... The following primary antibodies were used: 1:2000 mouse anti-GFP (RRID:AB_2313808, 632381, Clontech); 1:2000 mouse anti-3V5 (RRID:AB_2556564 ...
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Enterohepatic Transcription Factor CREB3L3 Protects Atherosclerosis via SREBP Competitive InhibitionbioRxiv - Physiology 2020Quote: ... GFP-tagged full-length mouse Creb3l3 cDNA was inserted into pEGFP (GFP-CREB3L3) (Clontech), mCherry-tagged human SREBP-1c was inserted into pmCherry (mCherry-SREBP-1c ...
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bioRxiv - Molecular Biology 2023Quote: ... GFP fusion proteins were revealed with mouse monoclonal antibody anti-GFP (JL-8, Clontech) used at 1/2000 dilution ...
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bioRxiv - Cell Biology 2023Quote: Total RNAs of mouse tissues were extracted by TRIzol reagent (Takara Bio, Tokyo, Japan), then were used for cDNA synthesis with HiScript® II Q Select RT SuperMix for qPCR (Vazyme Biotech Co. ...
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bioRxiv - Cell Biology 2023Quote: cDNAs of mouse Upf3a and Upf3b were amplified with PrimeSTAR® HS Premix (Takara) from an E14.1 ES cell cDNA library ...
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bioRxiv - Cell Biology 2023Quote: ... The following primary antibodies were used: mouse anti-GFP JL-8 (1:1000; TaKaRa), mouse anti-Tubulin AA4.3-c (1:5000 ...
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bioRxiv - Neuroscience 2023Quote: ... the mouse monoclonal anti-GFP (Jl-8, Clontech; 1:500 overnight at 4°C) primary antibody was used ...
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bioRxiv - Cell Biology 2023Quote: ... The following primary antibody was used in this study: mouse anti-GFP (JL8, Takara). Goat anti-mouse (A32730 ...
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bioRxiv - Biochemistry 2020Quote: ... pOBD plasmids were then transformed into PJ69-4α yeast and were selected on tryptophan-dropout medium (Clontech, Mountain View CA, #630413); pGAD plasmids were transformed into PJ49-4a yeast and were selected on leucine-dropout medium (Clontech ...
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bioRxiv - Cell Biology 2019Quote: ... LW2A and YKLW4A were cloned from plasmid pECerulean (37) to pHH21 with In-Fusion® HD Cloning Kit (Takara Bio, Japan). Recombinant Influenza viruses were produced with the twelve plasmids system (38 ...
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bioRxiv - Immunology 2019Quote: ... conjugated to vinculin or the vinculin A50I mutant were cloned into the doxycycline-inducible Tet-On 3G plasmid system (Takara Bio). HoxB8-conditional progenitors (expressing Cas9 and control sgRNA or Vcl (2 ...
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bioRxiv - Plant Biology 2020Quote: ... and the BrSCR-46 cassette was similarly cloned into the plasmid containing the BrSRK-46 chimera by InFusion cloning (TAKARA Bio). Similarly ...
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bioRxiv - Biochemistry 2020Quote: ... The PfNEDD8 PCR product was cloned into the StuI-HindIII digested pSMO plasmid using in-Fusion® HD Cloning Plus kit (Takara) and transformed into DH5α E ...